Long-chain-fatty-acid--CoA ligase 3 (P39002)

Identification

Name

Long-chain-fatty-acid--CoA ligase 3

Synonyms

Fatty acid activator 3
Long-chain acyl-CoA synthetase 3

Gene Name

FAA3

Enzyme Class

6.2.1.3

Biological Properties

General Function

Involved in catalytic activity

Specific Function

Esterification, concomitant with transport, of endogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. This enzyme acts preferentially on C16 and C18 fatty acids with a cis-double bond at C-9-C-10

Cellular Location

Not Available

SMPDB Pathways

Biosynthesis of unsaturated fatty acids	PW002403
Biosynthesis of unsaturated fatty acids (docosanoyl)	PW002408
Biosynthesis of unsaturated fatty acids (tetracosanoyl-CoA)	PW002404
Fatty acid metabolism	PW002460

KEGG Pathways

Biosynthesis of unsaturated fatty acids	ec01040
Fatty acid metabolism	ec00071

SMPDB Reactions

Palmitic acid + Adenosine triphosphate + Coenzyme A → Adenosine monophosphate + Pyrophosphate + Palmityl-CoA

KEGG Reactions

Adenosine triphosphate + myristic acid + Coenzyme A ↔ Adenosine monophosphate + Pyrophosphate + Tetradecanoyl-CoA

Adenosine triphosphate + Myristoleic acid + Coenzyme A ↔ Adenosine monophosphate + Pyrophosphate

Metabolites

YMDB ID	Name	View
YMDB00045	Coenzyme A	Show
YMDB00069	Palmitic acid	Show
YMDB00097	Adenosine monophosphate	Show
YMDB00109	Adenosine triphosphate	Show
YMDB00113	4-Phospho-L-aspartic acid	Show
YMDB00219	Pyrophosphate	Show
YMDB00301	Palmityl-CoA	Show
YMDB00469	hexadec-2-enoyl-CoA	Show
YMDB00527	palmitoyl-CoA	Show
YMDB00528	myristoyl-CoA	Show
YMDB00538	stearoyl-CoA	Show
YMDB00539	oleoyl-CoA	Show
YMDB00679	myristic acid	Show
YMDB00680	Myristoleic acid	Show
YMDB00681	palmitoleic acid	Show
YMDB00682	stearic acid	Show
YMDB00884	Linoleic acid	Show

GO Classification

Component
Not Available
Function
catalytic activity
Process
metabolic process

Gene Properties

Chromosome Location

chromosome 9

Locus

YIL009W

Gene Sequence

>2085 bp ATGTCCGAACAACACTCTGTCGCAGTCGGTAAAGCTGCTAATGAGCACGAGACTGCCCCT AGGAGAAATGTTAGAGTCAAGAAGCGGCCCTTAATTAGACCATTGAACTCGTCAGCATCT ACGCTGTATGAATTTGCCCTAGAGTGTTTCAACAAGGGTGGAAAACGAGATGGTATGGCT TGGAGAGATGTCATCGAGATTCATGAGACAAAGAAAACCATTGTGAGAAAGGTAGACGGC AAGGATAAATCTATAGAAAAGACATGGCTGTATTATGAAATGTCACCATATAAAATGATG ACCTACCAGGAACTGATCTGGGTGATGCACGATATGGGCCGTGGGCTGGCAAAAATAGGC ATCAAGCCCAATGGAGAACACAAATTCCACATCTTCGCATCTACTTCCCATAAATGGATG AAGATTTTCCTTGGTTGCATATCCCAGGGTATCCCCGTAGTAACCGCGTATGATACTTTG GGTGAGAGCGGTTTGATTCACTCCATGGTTGAAACCGAGTCTGCTGCTATTTTCACTGAT AATCAATTATTGGCTAAAATGATAGTGCCTTTGCAATCTGCTAAAGATATCAAATTTCTT ATCCATAACGAACCTATCGACCCCAATGACAGAAGACAAAACGGCAAACTTTACAAGGCT GCTAAGGATGCCATTAATAAGATCAGAGAAGTTAGGCCAGACATAAAAATTTATAGTTTT GAAGAAGTTGTCAAGATAGGTAAAAAAAGTAAAGATGAGGTCAAACTTCATCCACCTGAG CCAAAAGATTTGGCTTGTATCATGTACACCTCGGGCTCGATCAGTGCACCAAAAGGTGTA GTATTGACTCATTATAATATTGTTTCGGGTATCGCTGGTGTAGGTCACAACGTCTTTGGA TGGATCGGCTCTACAGACCGTGTTTTGTCGTTCTTGCCATTGGCTCATATTTTTGAACTG GTCTTTGAATTCGAAGCCTTTTACTGGAACGGTATTCTTGGGTACGGTAGTGTTAAGACT TTGACTAATACTTCGACTCGTAATTGTAAGGGTGACCTGGTTGAGTTTAAGCCTACTATT ATGATCGGTGTGGCTGCCGTTTGGGAAACTGTGAGAAAAGCTATTTTGGAAAAGATCAGC GATTTAACTCCCGTACTCCAAAAGATTTTTTGGTCTGCCTATAGTATGAAAGAAAAGAGT GTACCATGCACCGGGTTTTTAAGTCGTATGGTCTTCAAGAAAGTCAGACAAGCCACCGGT GGTCATCTTAAGTATATTATGAACGGTGGGTCTGCGATCAGTATTGATGCTCAGAAATTC TTTTCTATCGTCCTGTGTCCTATGATTATCGGTTACGGCCTTACTGAAACAGTTGCGAAT GCTTGTGTTTTGGAGCCTGATCATTTCGAATATGGTATAGTTGGTGATCTTGTTGGATCG GTCACTGCCAAATTGGTGGATGTTAAGGACCTAGGTTATTATGCAAAAAACAATCAAGGT GAATTGCTTCTAAAGGGTGCGCCGGTCTGTTCTGAATATTATAAGAATCCAATAGAAACG GCGGTCTCTTTCACTTACGATGGATGGTTTCGTACTGGTGATATTGTTGAATGGACTCCC AAGGGACAACTTAAAATTATTGATAGAAGAAAGAATTTGGTTAAAACCCTAAATGGTGAA TATATTGCATTAGAAAAGTTAGAATCTGTTTACAGGTCAAACTCCTATGTGAAAAATATC TGTGTTTATGCCGATGAAAGTAGGGTTAAACCGGTGGGTATTGTGGTACCCAACCCAGGA CCCCTATCTAAATTTGCTGTCAAATTGCGTATTATGAAAAAGGGTGAAGACATCGAAAAC TATATCCATGACAAAGCATTACGAAATGCTGTTTTCAAAGAGATGATCGCAACAGCCAAA TCTCAAGGTTTGGTTGGTATTGAACTATTATGTGGTATTGTTTTCTTTGATGAAGAATGG ACACCTGAAAATGGCTTTGTCACATCTGCTCAAAAATTAAAGAGAAGAGAAATCTTAGCC GCTGTTAAATCAGAAGTCGAAAGGGTTTACAAAGAAAATTCTTAG

Protein Properties

Pfam Domain Function

AMP-binding (PF00501 )

Protein Residues

694

Protein Molecular Weight

77946.0

Protein Theoretical pI

9.41

Signalling Regions

None

Transmembrane Regions

None

Protein Sequence

>Long-chain-fatty-acid--CoA ligase 3 MSEQHSVAVGKAANEHETAPRRNVRVKKRPLIRPLNSSASTLYEFALECFNKGGKRDGMA WRDVIEIHETKKTIVRKVDGKDKSIEKTWLYYEMSPYKMMTYQELIWVMHDMGRGLAKIG IKPNGEHKFHIFASTSHKWMKIFLGCISQGIPVVTAYDTLGESGLIHSMVETESAAIFTD NQLLAKMIVPLQSAKDIKFLIHNEPIDPNDRRQNGKLYKAAKDAINKIREVRPDIKIYSF EEVVKIGKKSKDEVKLHPPEPKDLACIMYTSGSISAPKGVVLTHYNIVSGIAGVGHNVFG WIGSTDRVLSFLPLAHIFELVFEFEAFYWNGILGYGSVKTLTNTSTRNCKGDLVEFKPTI MIGVAAVWETVRKAILEKISDLTPVLQKIFWSAYSMKEKSVPCTGFLSRMVFKKVRQATG GHLKYIMNGGSAISIDAQKFFSIVLCPMIIGYGLTETVANACVLEPDHFEYGIVGDLVGS VTAKLVDVKDLGYYAKNNQGELLLKGAPVCSEYYKNPIETAVSFTYDGWFRTGDIVEWTP KGQLKIIDRRKNLVKTLNGEYIALEKLESVYRSNSYVKNICVYADESRVKPVGIVVPNPG PLSKFAVKLRIMKKGEDIENYIHDKALRNAVFKEMIATAKSQGLVGIELLCGIVFFDEEW TPENGFVTSAQKLKRREILAAVKSEVERVYKENS

References

External Links

Resource	Link
Saccharomyces Genome Database	FAA3
Uniprot ID	P39002
Uniprot Name	LCF3_YEAST
GenBank Gene ID	AY558110
Genebank Protein ID	45270110

General Reference

Johnson, D. R., Knoll, L. J., Rowley, N., Gordon, J. I. (1994). "Genetic analysis of the role of Saccharomyces cerevisiae acyl-CoA synthetase genes in regulating protein N-myristoylation." J Biol Chem 269:18037-18046.8027063
Churcher, C., Bowman, S., Badcock, K., Bankier, A., Brown, D., Chillingworth, T., Connor, R., Devlin, K., Gentles, S., Hamlin, N., Harris, D., Horsnell, T., Hunt, S., Jagels, K., Jones, M., Lye, G., Moule, S., Odell, C., Pearson, D., Rajandream, M., Rice, P., Rowley, N., Skelton, J., Smith, V., Barrell, B., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Nature 387:84-87.9169870
Hu, Y., Rolfs, A., Bhullar, B., Murthy, T. V., Zhu, C., Berger, M. F., Camargo, A. A., Kelley, F., McCarron, S., Jepson, D., Richardson, A., Raphael, J., Moreira, D., Taycher, E., Zuo, D., Mohr, S., Kane, M. F., Williamson, J., Simpson, A., Bulyk, M. L., Harlow, E., Marsischky, G., Kolodner, R. D., LaBaer, J. (2007). "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Genome Res 17:536-543.17322287
Knoll, L. J., Johnson, D. R., Gordon, J. I. (1994). "Biochemical studies of three Saccharomyces cerevisiae acyl-CoA synthetases, Faa1p, Faa2p, and Faa3p." J Biol Chem 269:16348-16356.8206942
Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106