Pyruvate dehydrogenase complex protein X component, mitochondrial (P16451)

Identification
NamePyruvate dehydrogenase complex protein X component, mitochondrial
Synonyms
  • Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex
  • E3-binding protein
  • Pyruvate dehydrogenase complex component E3BP
Gene NamePDX1
Enzyme ClassNot Available
Biological Properties
General FunctionEnergy production and conversion
Specific FunctionRequired for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex
Cellular LocationMitochondrion matrix
SMPDB Pathways
Citric Acid Cycle 1434561204PW000970 ThumbThumb?image type=greyscaleThumb?image type=simple
Pyruvate metabolismPW002447 ThumbThumb?image type=greyscaleThumb?image type=simple
TCA CyclePW002377 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Pyruvate metabolismec00620 Map00620
SMPDB Reactions
Pyruvic acid + Coenzyme A + NAD → Acetyl-CoA + Carbon dioxide + NADH
Pyruvic acid + Thiamine pyrophosphate → Carbon dioxide + 2-(a-Hydroxyethyl)thiamine diphosphate
2-(a-Hydroxyethyl)thiamine diphosphate + Lipoamide-E ↔ Thiamine pyrophosphate + S-Acetyldihydrolipoamide-E
Coenzyme A + S-Acetyldihydrolipoamide-E ↔ Acetyl-CoA + Dihydrolipoamide-E
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00045Coenzyme AShow
YMDB00110NADShow
YMDB00143NADHShow
YMDB00175Pyruvic acidShow
YMDB00312Acetyl-CoAShow
YMDB00381Thiamine pyrophosphateShow
YMDB00912Carbon dioxideShow
YMDB161662-(a-Hydroxyethyl)thiamine diphosphateShow
GO Classification
Function
protein binding
binding
catalytic activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
acyltransferase activity
Process
metabolic process
Gene Properties
Chromosome LocationNot Available
Locus
Gene Sequence>1233 bp ATGCTAAGTGCAATTTCCAAAGTCTCCACTTTAAAATCATGTACAAGATATTTAACCAAA TGCAACTATCATGCATCAGCTAAATTACTTGCTGTAAAGACATTTTCAATGCCTGCAATG TCTCCTACTATGGAGAAAGGGGGGATTGTGTCTTGGAAATATAAAGTTGGCGAACCATTC AGCGCGGGCGATGTGATATTAGAAGTGGAAACAGATAAATCTCAAATTGATGTGGAAGCA CTGGACGATGGTAAACTAGCTAAGATCCTGAAAGATGAAGGCTCTAAAGATGTTGATGTT GGTGAACCTATTGCTTATATTGCTGATGTTGATGATGATTTAGCTACTATAAAGTTACCC CAAGAGGCCAACACCGCAAATGCGAAATCTATTGAAATTAAGAAGCCATCCGCAGATAGT ACTGAAGCAACACAACAACATTTAAAAAAAGCCACAGTTACACCAATAAAAACCGTTGAC GGCAGCCAAGCCAATCTTGAACAGACGCTATTACCATCCGTGTCATTACTACTGGCTGAG AACAATATATCCAAACAAAAGGCTTTGAAGGAAATTGCGCCATCTGGTTCCAACGGTAGA CTATTAAAGGGTGATGTGCTAGCATACCTAGGGAAAATACCACAAGATTCGGTTAACAAG GTAACAGAATTTATCAAGAAGAACGAACGTCTCGATTTATCGAACATTAAACCTATACAG CTCAAACCAAAAATAGCCGAGCAAGCTCAAACAAAAGCTGCCGACAAGCCAAAGATTACT CCTGTAGAATTTGAAGAGCAATTAGTGTTCCATGCTCCCGCCTCTATTCCGTTTGACAAA CTGAGTGAATCATTGAACTCTTTCATGAAAGAAGCTTACCAGTTCTCACACGGAACACCA CTAATGGACACAAATTCGAAATACTTTGACCCTATTTTCGAGGACCTTGTCACCTTGAGC CCAAGAGAGCCAAGATTTAAATTTTCCTATGACTTGATGCAAATTCCCAAAGCTAATAAC ATGCAAGACACGTACGGTCAAGAAGACATATTTGACCTCTTAACAGGTTCAGACGCGACT GCCTCATCAGTAAGACCCGTTGAAAAGAACTTACCTGAAAAAAACGAATATATACTAGCG TTGAATGTTAGCGTCAACAACAAGAAGTTTAATGACGCGGAGGCCAAGGCAAAAAGATTC CTTGATTACGTAAGGGAGTTAGAATCATTTTGA
Protein Properties
Pfam Domain Function
Protein Residues410
Protein Molecular Weight45361.30078
Protein Theoretical pI5.41
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Pyruvate dehydrogenase complex protein X component, mitochondrial MLSAISKVSTLKSCTRYLTKCNYHASAKLLAVKTFSMPAMSPTMEKGGIVSWKYKVGEPF SAGDVILEVETDKSQIDVEALDDGKLAKILKDEGSKDVDVGEPIAYIADVDDDLATIKLP QEANTANAKSIEIKKPSADSTEATQQHLKKATVTPIKTVDGSQANLEQTLLPSVSLLLAE NNISKQKALKEIAPSGSNGRLLKGDVLAYLGKIPQDSVNKVTEFIKKNERLDLSNIKPIQ LKPKIAEQAQTKAADKPKITPVEFEEQLVFHAPASIPFDKLSESLNSFMKEAYQFSHGTP LMDTNSKYFDPIFEDLVTLSPREPRFKFSYDLMQIPKANNMQDTYGQEDIFDLLTGSDAT ASSVRPVEKNLPEKNEYILALNVSVNNKKFNDAEAKAKRFLDYVRELESF
References
External Links
ResourceLink
Saccharomyces Genome Database PDX1
Uniprot IDP16451
Uniprot NameODPX_YEAST
GenBank Gene IDM28222
Genebank Protein ID172268
General Reference
  • Behal, R. H., Browning, K. S., Hall, T. B., Reed, L. J. (1989). "Cloning and nucleotide sequence of the gene for protein X from Saccharomyces cerevisiae." Proc Natl Acad Sci U S A 86:8732-8736.2682658
  • Arroyo, J., Garcia-Gonzalez, M., Garcia-Saez, M. I., Sanchez, M., Nombela, C. (1995). "The complete sequence of a 9037 bp DNA fragment of the right arm of Saccharomyces cerevisiae chromosome VII." Yeast 11:587-591.7645350
  • Tettelin, H., Agostoni Carbone, M. L., Albermann, K., Albers, M., Arroyo, J., Backes, U., Barreiros, T., Bertani, I., Bjourson, A. J., Bruckner, M., Bruschi, C. V., Carignani, G., Castagnoli, L., Cerdan, E., Clemente, M. L., Coblenz, A., Coglievina, M., Coissac, E., Defoor, E., Del Bino, S., Delius, H., Delneri, D., de Wergifosse, P., Dujon, B., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Nature 387:81-84.9169869
  • Lawson, J. E., Behal, R. H., Reed, L. J. (1991). "Disruption and mutagenesis of the Saccharomyces cerevisiae PDX1 gene encoding the protein X component of the pyruvate dehydrogenase complex." Biochemistry 30:2834-2839.2007123
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106