Putative dehydrodolichyl diphosphate synthase (Q03175)

Identification
NamePutative dehydrodolichyl diphosphate synthase
Synonyms
  • DEDOL-PP synthase
Gene NameSRT1
Enzyme Class
Biological Properties
General FunctionInvolved in transferase activity, transferring alkyl or aryl (other than methyl) groups
Specific FunctionCould be a cis-prenyl transferase that adds multiple copies of isopentenyl pyrophosphate (ipp) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (DeDol- PP)
Cellular LocationNot Available
SMPDB Pathways
Terpenoid backbone biosynthesisPW002485 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Terpenoid backbone biosynthesisec00900 Map00900
SMPDB Reactions
Farnesyl pyrophosphate + Isopentenyl pyrophosphate → di-trans, poly-cis-polyprenyl diphosphate (C80) + Pyrophosphate
KEGG Reactions
Farnesyl pyrophosphate + Isopentenyl pyrophosphate ↔ Pyrophosphate + geranylgeranyl diphosphate
Isopentenyl pyrophosphate + geranylgeranyl diphosphate ↔ Pyrophosphate + pentaprenyl diphosphate + hydron
pentaprenyl diphosphate + Isopentenyl pyrophosphate ↔ Pyrophosphate + hexaprenyl diphosphate
hexaprenyl diphosphate + Isopentenyl pyrophosphate + hydron ↔ Pyrophosphate + heptaprenyl diphosphate
heptaprenyl diphosphate + Isopentenyl pyrophosphate ↔ Pyrophosphate + octaprenyl diphosphate
Metabolites
YMDB IDNameView
YMDB00200Isopentenyl pyrophosphateShow
YMDB00219PyrophosphateShow
YMDB00229Farnesyl pyrophosphateShow
YMDB00614nonaprenyl diphosphateShow
YMDB00631geranylgeranyl diphosphateShow
YMDB00632pentaprenyl diphosphateShow
YMDB00633hexaprenyl diphosphateShow
YMDB00634heptaprenyl diphosphateShow
YMDB00635octaprenyl diphosphateShow
YMDB00636decaprenyl diphosphateShow
YMDB00637undecaprenyl diphosphateShow
YMDB00638dodecaprenyl diphosphateShow
YMDB00639tridecaprenyl diphosphateShow
YMDB00640tetradecaprenyl diphosphateShow
YMDB00641pentadecaprenyl diphosphateShow
YMDB00642hexadecaprenyl diphosphateShow
YMDB00643heptadecaprenyl diphosphateShow
YMDB00644octadecaprenyl diphosphateShow
YMDB00645nonadecaprenyl diphosphateShow
YMDB00646icosaprenyl diphosphateShow
YMDB00647henicosaprenyl diphosphateShow
YMDB00648docosaprenyl diphosphateShow
YMDB00862hydronShow
YMDB16322di-trans, poly-cis-polyprenyl diphosphate (C80)Show
GO Classification
Component
Not Available
Function
catalytic activity
transferase activity
transferase activity, transferring alkyl or aryl (other than methyl) groups
Process
Not Available
Gene Properties
Chromosome Locationchromosome 13
LocusYMR101C
Gene Sequence>1032 bp ATGAAAATGCCCAGTATTATTCAGATTCAGTTTGTAGCCCTAAAAAGGCTTTTGGTAGAA ACCAAAGAACAGATGTGCTTCGCAGTGAAAAGTATATTTCAGAGAGTATTTGCGTGGGTT ATGTCATTAAGCTTGTTTTCATGGTTTTATGTAAATCTTCAGAATATTTTGATAAAAGCA TTAAGGGTAGGGCCAGTGCCTGAACATGTCTCCTTTATCATGGATGGTAACCGGAGATAT GCCAAGTCAAGAAGGCTACCAGTAAAAAAAGGCCATGAAGCTGGTGGGTTAACGTTACTA ACACTACTGTATATCTGCAAAAGATTGGGTGTAAAATGTGTTTCCGCCTATGCATTTTCT ATTGAAAATTTTAATAGACCAAAAGAAGAAGTAGATACGCTAATGAATTTGTTTACGGTA AAGCTTGATGAATTCGCAAAAAGAGCCAAGGACTATAAGGATCCCTTATACGGATCTAAA ATAAGAATAGTAGGTGATCAATCTTTACTATCTCCAGAAATGAGAAAAAAAATTAAAAAA GTGGAAGAAATCACACAGGATGGAGACGATTTCACTTTATTTATATGTTTTCCTTACACT TCAAGAAATGATATGTTACATACTATTCGTGATTCAGTTGAAGACCATTTGGAAAATAAA TCACCAAGGATTAATATAAGAAAATTTACTAATAAAATGTACATGGGTTTCCATTCCAAT AAATGTGAATTATTAATCAGAACAAGTGGGCATAGGAGGCTCTCAGACTATATGCTATGG CAAGTACATGAAAATGCCACCATTGAATTTAGTGATACGTTGTGGCCAAATTTTAGCTTC TTTGCTATGTACCTGATGATTCTCAAATGGTCCTTCTTTTCCACCATTCAAAAATATAAT GAGAAGAATCACTCATTGTTTGAAAAAATACATGAAAGCGTTCCTTCAATATTTAAAAAA AAGAAAACAGCTATGTCTTTGTACAACTTTCCAAACCCCCCCATTTCAGTTTCGGTTACA GGAGATGAATAA
Protein Properties
Pfam Domain Function
Protein Residues343
Protein Molecular Weight40199.69922
Protein Theoretical pI10.03
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Putative dehydrodolichyl diphosphate synthase MKMPSIIQIQFVALKRLLVETKEQMCFAVKSIFQRVFAWVMSLSLFSWFYVNLQNILIKA LRVGPVPEHVSFIMDGNRRYAKSRRLPVKKGHEAGGLTLLTLLYICKRLGVKCVSAYAFS IENFNRPKEEVDTLMNLFTVKLDEFAKRAKDYKDPLYGSKIRIVGDQSLLSPEMRKKIKK VEEITQDGDDFTLFICFPYTSRNDMLHTIRDSVEDHLENKSPRINIRKFTNKMYMGFHSN KCELLIRTSGHRRLSDYMLWQVHENATIEFSDTLWPNFSFFAMYLMILKWSFFSTIQKYN EKNHSLFEKIHESVPSIFKKKKTAMSLYNFPNPPISVSVTGDE
References
External Links
ResourceLink
Saccharomyces Genome Database SRT1
Uniprot IDQ03175
Uniprot NameSRT1_YEAST
GenBank Gene IDAB013498
Genebank Protein ID4126459
General Reference
  • Sato, M., Sato, K., Nishikawa, S., Hirata, A., Kato, J., Nakano, A. (1999). "The yeast RER2 gene, identified by endoplasmic reticulum protein localization mutations, encodes cis-prenyltransferase, a key enzyme in dolichol synthesis." Mol Cell Biol 19:471-483.9858571
  • Bowman, S., Churcher, C., Badcock, K., Brown, D., Chillingworth, T., Connor, R., Dedman, K., Devlin, K., Gentles, S., Hamlin, N., Hunt, S., Jagels, K., Lye, G., Moule, S., Odell, C., Pearson, D., Rajandream, M., Rice, P., Skelton, J., Walsh, S., Whitehead, S., Barrell, B. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Nature 387:90-93.9169872