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Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (P38179)

Identification
NameDolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase
Synonyms
  • Asparagine-linked glycosylation protein 3
  • Dol-P-Man-dependent alpha(1-3)-mannosyltransferase
  • Dolichyl-phosphate-mannose--glycolipid alpha-mannosyltransferase
  • HM-1 killer toxin resistance protein
Gene NameALG3
Enzyme Class
Biological Properties
General FunctionInvolved in transferase activity, transferring hexosyl groups
Specific FunctionAdds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man(5)GlcNAc(2)-PP-Dol. Sensitive to H.mrakii HM-1 killer toxin
Cellular LocationEndoplasmic reticulum membrane; Multi-pass membrane protein (Probable)
SMPDB Pathways
N-Glycan biosynthesisPW002501 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
N-Glycan biosynthesisec00510 Map00510
SMPDB Reactions
(Mannosyl)5-(N-acetylglucosaminyl)2-diphosphodolichol + dolichyl β-D-mannosyl phosphate → Dolichol phosphate + hydron + (Mannosyl)6-(N-acetylglucosaminyl)2-diphosphodolichol
KEGG Reactions
glycan G00006 + dolichyl D-mannosyl phosphate ↔ glycan G10595
Metabolites
YMDB IDNameView
YMDB00862hydronShow
YMDB00864Dolichol phosphateShow
YMDB00937dolichyl D-mannosyl phosphateShow
YMDB00956glycan G00006Show
YMDB00957glycan G10595Show
YMDB16227(Mannosyl)5-(N-acetylglucosaminyl)2-diphosphodolicholShow
YMDB16228(Mannosyl)6-(N-acetylglucosaminyl)2-diphosphodolicholShow
YMDB16337dolichyl β-D-mannosyl phosphateShow
GO Classification
Component
cell part
membrane part
intrinsic to membrane
integral to membrane
organelle
membrane-bounded organelle
intracellular membrane-bounded organelle
endoplasmic reticulum
Function
transferase activity, transferring hexosyl groups
catalytic activity
transferase activity
transferase activity, transferring glycosyl groups
Process
Not Available
Gene Properties
Chromosome Locationchromosome 2
LocusYBL082C
Gene Sequence>1377 bp ATGGAAGGTGAACAGTCTCCGCAAGGTGAAAAGTCTCTGCAAAGGAAGCAATTTGTCAGA CCTCCGCTGGATCTGTGGCAGGATCTCAAGGACGGTGTGCGCTACGTGATCTTCGATTGT AGGGCCAATCTTATCGTTATGCCCCTTTTGATTTTGTTCGAAAGCATGCTGTGCAAGATT ATCATTAAGAAGGTAGCTTACACAGAGATCGATTACAAGGCGTACATGGAGCAGATCGAG ATGATTCAGCTCGATGGCATGCTGGACTACTCTCAGGTGAGTGGTGGAACGGGCCCGCTG GTGTATCCAGCAGGCCACGTCTTGATCTACAAGATGATGTACTGGCTAACAGAGGGAATG GACCACGTTGAGCGCGGGCAAGTGTTTTTCAGATACTTGTATCTCCTTACACTGGCGTTA CAAATGGCGTGTTACTACCTTTTACATCTACCACCGTGGTGTGTGGTCTTGGCGTGCCTC TCTAAAAGATTGCACTCTATTTACGTGCTACGGTTATTCAATGATTGCTTCACTACTTTG TTTATGGTCGTCACGGTTTTGGGGGCTATCGTGGCCAGCAGGTGCCATCAGCGCCCCAAA TTAAAGAAGTCCCTTGCGCTGGTGATCTCCGCAACATACAGTATGGCTGTGAGCATTAAG ATGAATGCGCTGTTGTATTTCCCTGCAATGATGATTTCTCTATTCATCCTTAATGACGCG AACGTAATCCTTACTTTGTTGGATCTCGTTGCGATGATTGCATGGCAAGTCGCAGTTGCA GTGCCCTTCCTGCGCAGCTTTCCGCAACAGTACCTGCATTGCGCTTTTAATTTCGGCAGG AAGTTTATGTACCAATGGAGTATCAATTGGCAAATGATGGATGAAGAGGCTTTCAATGAT AAGAGGTTCCACTTGGCCCTTTTAATCAGCCACCTGATAGCGCTCACCACACTGTTCGTC ACAAGATACCCTCGCATCCTGCCCGATTTATGGTCTTCCCTGTGCCATCCGCTGAGGAAA AATGCAGTGCTCAATGCCAATCCCGCCAAGACTATTCCATTCGTTCTAATCGCATCCAAC TTCATCGGCGTCCTATTTTCAAGGTCCCTCCACTACCAGTTTCTATCCTGGTATCACTGG ACTTTGCCTATACTGATCTTTTGGTCGGGAATGCCCTTCTTCGTTGGTCCCATTTGGTAC GTCTTGCACGAGTGGTGCTGGAATTCCTATCCACCAAACTCACAAGCAAGCACGCTATTG TTGGCATTGAATACTGTTCTGTTGCTTCTATTGGCCTTGACGCAGCTATCTGGTTCGGTC GCCCTCGCCAAAAGCCATCTTCGTACCACCAGCTCTATGGAAAAAAAGCTCAACTGA
Protein Properties
Pfam Domain Function
Protein Residues458
Protein Molecular Weight52860.39844
Protein Theoretical pI9.09
Signalling Regions
  • None
Transmembrane Regions
  • 42-62
  • 96-116
  • 138-158
  • 172-192
  • 217-237
  • 243-263
  • 303-323
  • 346-366
  • 382-402
  • 416-436
Protein Sequence>Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase MEGEQSPQGEKSLQRKQFVRPPLDLWQDLKDGVRYVIFDCRANLIVMPLLILFESMLCKI IIKKVAYTEIDYKAYMEQIEMIQLDGMLDYSQVSGGTGPLVYPAGHVLIYKMMYWLTEGM DHVERGQVFFRYLYLLTLALQMACYYLLHLPPWCVVLACLSKRLHSIYVLRLFNDCFTTL FMVVTVLGAIVASRCHQRPKLKKSLALVISATYSMAVSIKMNALLYFPAMMISLFILNDA NVILTLLDLVAMIAWQVAVAVPFLRSFPQQYLHCAFNFGRKFMYQWSINWQMMDEEAFND KRFHLALLISHLIALTTLFVTRYPRILPDLWSSLCHPLRKNAVLNANPAKTIPFVLIASN FIGVLFSRSLHYQFLSWYHWTLPILIFWSGMPFFVGPIWYVLHEWCWNSYPPNSQASTLL LALNTVLLLLLALTQLSGSVALAKSHLRTTSSMEKKLN
References
External Links
ResourceLink
Saccharomyces Genome Database ALG3
Uniprot IDP38179
Uniprot NameALG3_YEAST
GenBank Gene IDX79489
Genebank Protein ID496697
General Reference
  • Obermaier, B., Gassenhuber, J., Piravandi, E., Domdey, H. (1995). "Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II." Yeast 11:1103-1112.7502586
  • Feldmann, H., Aigle, M., Aljinovic, G., Andre, B., Baclet, M. C., Barthe, C., Baur, A., Becam, A. M., Biteau, N., Boles, E., Brandt, T., Brendel, M., Bruckner, M., Bussereau, F., Christiansen, C., Contreras, R., Crouzet, M., Cziepluch, C., Demolis, N., Delaveau, T., Doignon, F., Domdey, H., Dusterhus, S., Dubois, E., Dujon, B., El Bakkoury, M., Entian, K. D., Feurmann, M., Fiers, W., Fobo, G. M., Fritz, C., Gassenhuber, H., Glandsdorff, N., Goffeau, A., Grivell, L. A., de Haan, M., Hein, C., Herbert, C. J., Hollenberg, C. P., Holmstrom, K., Jacq, C., Jacquet, M., Jauniaux, J. C., Jonniaux, J. L., Kallesoe, T., Kiesau, P., Kirchrath, L., Kotter, P., Korol, S., Liebl, S., Logghe, M., Lohan, A. J., Louis, E. J., Li, Z. Y., Maat, M. J., Mallet, L., Mannhaupt, G., Messenguy, F., Miosga, T., Molemans, F., Muller, S., Nasr, F., Obermaier, B., Perea, J., Pierard, A., Piravandi, E., Pohl, F. M., Pohl, T. M., Potier, S., Proft, M., Purnelle, B., Ramezani Rad, M., Rieger, M., Rose, M., Schaaff-Gerstenschlager, I., Scherens, B., Schwarzlose, C., Skala, J., Slonimski, P. P., Smits, P. H., Souciet, J. L., Steensma, H. Y., Stucka, R., Urrestarazu, A., van der Aart, Q. J., van Dyck, L., Vassarotti, A., Vetter, I., Vierendeels, F., Vissers, S., Wagner, G., de Wergifosse, P., Wolfe, K. H., Zagulski, M., Zimmermann, F. K., Mewes, H. W., Kleine, K. (1994). "Complete DNA sequence of yeast chromosome II." EMBO J 13:5795-5809.7813418
  • Cusick, M. E. (1994). "Sequence of a segment of yeast chromosome II shows two novel genes, one almost entirely hydrophobic and the other extremely asparagine-serine rich." Yeast 10:1251-1256.7754714
  • Kimura, T., Kitamoto, N., Kito, Y., Iimura, Y., Shirai, T., Komiyama, T., Furuichi, Y., Sakka, K., Ohmiya, K. (1997). "A novel yeast gene, RHK1, is involved in the synthesis of the cell wall receptor for the HM-1 killer toxin that inhibits beta-1,3-glucan synthesis." Mol Gen Genet 254:139-147.9108275
  • Aebi, M., Gassenhuber, J., Domdey, H., te Heesen, S. (1996). "Cloning and characterization of the ALG3 gene of Saccharomyces cerevisiae." Glycobiology 6:439-444.8842708
  • Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258