1-phosphatidylinositol-3-phosphate 5-kinase FAB1 (P34756)

Identification

Name

1-phosphatidylinositol-3-phosphate 5-kinase FAB1

Synonyms

Phosphatidylinositol-3-phosphate 5-kinase
Type III PIP kinase
PIPkin-III

Gene Name

FAB1

Enzyme Class

2.7.1.150

Biological Properties

General Function

Involved in ATP binding

Specific Function

The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol-3-phosphate on the fifth hydroxyl of the myo- inositol ring, to form phosphatidylinositol-3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. The product of the reaction it catalyzes functions as an important regulator of vacuole homeostasis perhaps by controlling membrane flux to and/or from the vacuole. Hyperosmotic shock-induced increase in the levels of PtdIns(3,5)P2 requires the presence of VAC7, VAC14, and/or FIG4

Cellular Location

Vacuole membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein.

SMPDB Pathways

Inositol Metabolism	PW002498
Inositol phosphate metabolism	PW002495

KEGG Pathways

Inositol phosphate metabolism

ec00562

SMPDB Reactions

1-phosphatidyl-1D-myo-inositol 4-phosphate + Adenosine triphosphate → 1-phosphatidyl-1D-myo-inositol 4,5-biphosphate + ADP

KEGG Reactions

Adenosine triphosphate + 1-phosphatidyl-1D-myo-inositol 3-phosphate + hydron ↔ ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate

Adenosine triphosphate + 1-phosphatidyl-1D-myo-inositol 3-phosphate → ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate

Metabolites

YMDB ID	Name	View
YMDB00109	Adenosine triphosphate	Show
YMDB00862	hydron	Show
YMDB00914	ADP	Show
YMDB16249	1-phosphatidyl-1D-myo-inositol 4-phosphate	Show
YMDB16251	1-phosphatidyl-1D-myo-inositol 4,5-biphosphate	Show

GO Classification

Component
Not Available
Function
lipid kinase activity
purine nucleoside binding
phosphatidylinositol phosphate kinase activity
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
ion binding
cation binding
metal ion binding
transition metal ion binding
kinase activity
catalytic activity
zinc ion binding
transferase activity
protein binding
transferase activity, transferring phosphorus-containing groups
binding
nucleoside binding
Process
phosphatidylinositol metabolic process
cellular protein metabolic process
metabolic process
organophosphate metabolic process
macromolecule metabolic process
phospholipid metabolic process
protein metabolic process
glycerophospholipid metabolic process
phosphoinositide metabolic process

Gene Properties

Chromosome Location

chromosome 6

Locus

YFR019W

Gene Sequence

>6837 bp ATGTCATCGGAAGAACCCCATGCATCTATATCCTTTCCAGATGGCTCACATGTCCGGTCG TCCTCCACTGGTACTTCATCTGTGAACACAATCGATGCTACCTTATCCAGACCGAACTAT ATTAAGAAACCGTCCCTGCACATTATGTCGACATCTACGACGTCGACTACGACGGATCTA GTGACAAACCCAATTCTGAGCAATATCTCCGTGCCAAAAATAAGCCCCCCCACATCATCC TCGATAGCAACAGCGACATCAACAAGTCACGTGACCGGCACTGCGTCGCATTCGAACATT AAAGCTAATGCTAACACTAGTACCAGCGTGAATAAGAAAAATTTGCCGCCAACAACTAGT GGGAGGATACCCTCTAGCACCATCAAGAGATATCCGAGTCGCTATAAGCCTTCTCACTCG CTGCAGCTGCCCATTAAGAACGACTCCAACTTTAAACGATCTAGCATATATGCCTCCAAA TCCACTGTTACTGCTATCCCCATTAGGAACAATCGGCCAATTTCCATGCAGAATTCCTAT GCGCGCACTCCTGACAGCGATCATGACGACGTTGGCGATGAAGTGTCTTCGATAAAGAGT GCCTCTTCTTCGTTGACCGCATCCCTGTCCAAGAGTTTCCTGTTCGCATTTTATAACAAC CGCAAGAAGGACAAGACCAGTAACAATGGTGTCCTCTCAAAGGAATATTGGATGAAGGAC GAGAGTTCTAAAGAATGTTTCAGTTGTGGTAAAACGTTTAACACTTTCAGAAGGAAACAC CACTGCAGGATATGCGGCCAAATTTTTTGCAGCAGTTGCACTCTCTTGATAGATGGTGAT CGATTCGGCTGTCATGCCAAGATGCGAGTATGTTACAATTGTTATGAGCATGCAGACACA TACGAGGACTCCAGTGATGAAGAGAATGATTCTACAATGCAATTGAATGAGCCAAGGTCT CGCTCTCGCTCTCGTTCTTCCAATACCAATCCATATTCTCATTCACATTCGCATTTGCAT CTCATTTCACAGGACAACCATAACGGTACCGATCTCCATGATCCAGTAGCAGCAACGGAT AACCCTCAACAGCAGAATGAGGTCTACCTATTAAATGACGATGACGTACAAAGTATAATG ACCTCAGGTGAAGATTCTAAATTGTTCATTTCTACACCCCCACCACCACCGAAGATGGCC ATCCCAGCAACAAAGCAAGGCGGATCGCTGGAAATCTCATTCGATTCGGAAAACGATAGG GCTTTGCATTATCAAGATGACAACCCCGGTCGCCATCATCACCTCGATTCCGTGCCCACA AGGTACACTATCAGAGATATGGACAATATATCACATTACGATACCAACTCTAATTCCACT TTGAGGCCACATTATAACACCAATAACAGCACTATTACCATCAACAACCTCAACAACACT ACTTCCAACAACAGCAACTATAACAATACCAATAGCAACAGCAACATCAATAATCCTGCG CATTCCTTAAGAAGGTCTATTTTCCATTATGTAAGCAGCAATAGTGTTAACAAGGACAGT AACAATAGCTCTGCAACACCCGCTTCGTCTGCACAGTCTTCTTCCATACTGGATCCTGCC AATCGTATTATCGGGAATTATGCTCATAGAAATTACAAGTTTAAATTCAACTACAATTCC AAAGGTCCTTCGCAACAAAATGACACGGCAAATGGAAATAATGATAATAATAATAATAAT AATAATAATAATAATAATAATAATAATAACAGTGCCAGCGGTATTGCTGACAACAATAAC ATTCCCTCAAACGACAATGGCACGACGTTCACTCTAGATAAGAAGAAAAGAAACCCACTG ACAAAATCTAAATCTACGTCCGCTTACCTAGAATATCCTCTAAACGAGGAAGACTCTTCT GAGGACGAAGGTTCCATGTCCATATATTCTGTATTGAATGATGACCATAAAACTGACAAT CCAATTAGATCAATGAGGAACTCCACAAAATCTTTTCAACGAGCGCAAGCTTCACTACAG CGGATGAGGTTTAGAAGGAAAAGTAAAAGCAAGCATTTTCCCAACAATTCGAAAAGTTCC ATTTATAGAGACTTAAATTTCTTGACCAATAGTACTCCCAATTTACTTTCTGTAGTAAGC GACGATAATCTATACGACGATTCTTCTCCTTTGCAAGACAAGGCCTCTTCATCCGCTGCA AGCAGACTCACTGACAGAAAATTCAGCAATAGCAGTGGCAGCAATAATAATAGCAATAGT AATAGCAATATCAACACCGATCCTTGGAAGAGAATCGCTAGTATATCGGGATTCAAACTG AAAAAGGAAAAAAAGAGAGAATTAAATGAAGTCTCTCTTTTACATATGCATGCCCTATTA AAACAATTATTGAATGATCAAGAAATTTCCAATCTTCAAGAATGGATAACCCTTCTAGAT GGCGCTTTGCGTAAAGTTTTAAGGACCATCCTGAACGCGAGGGATTTGAACACATTAGAT TTTAGACAAACTTACGTTAAGATAAAAAGAATTAGTGGTGGATCTCCTCAAAATTCAGAA TATATCGACGGGGTTGTCTTTTCGAAAGCATTGCCGTCGAAAACAATGCCACGGCATTTG AAAAACCCAAGGATACTATTAATCATGTTTCCACTGGAGTACCAAAAGAACAATAACCAC TTTCTAAGTATAGAGTCCGTCTTTAGGCAAGAGCGCGAATATTTGGATAAACTTGTTTCG AGATTAAAGTCATTACATCCTGATATTATATACGTCGGCGCCAATGTTAGTGGGTACGCG TTAGAACTATTAAATGACTCAGGCATTGTCGTACAGTTTAATATGAAACCACAAGTCATT GAAAGAATTGCCAAGCTAACAGAAGCAGATATTGCCATTTCTGTGGATAAGTTGGCTACG AATATTAAGATGGGTGAATGTGAAACTTTTGAAGTTAAAAGCTACATTTATGGAAACATC AGTAAAACATATACATTTCTTCGGGGCTGTAACCCAGAACTTGGTGGAACAATTTTGTTA AGAGGTGATAGTCTTGAAAACTTGAGAAAAATCAAACAAGTCTCTGAATTTATGGTCTAC GCAATTTTTTCATTGAAGTTGGAAAGTTCATTTTTTAACGATAATTTCATACAACTTTCT ACAGATGTTTATTTGAAGAGAGCTGAATCCAAAAAACTGCAAGTTTTCGAAGGTTATTTT GCAGATTTCTTGATCAAATTCAATAACAGGATTTTGACTGTATCACCAACGGTAGATTTC CCCATTCCCTTTTTATTGGAAAAAGCCAGAGGCTTGGAAAAGAAGCTCATAGAAAGGATC AATCAATATGAAAGCGAATCTGATTTAGATCGGCAAACGCAACTAAATATGCTTCAAGGC TTAGAATCGACTATCACTAAGAAACACTTAGGAAATTTAATAAAATTCCTGCATGAAATG GAAATTGAAAATTTAGAGCTTGAGTTTCAGAAGAGAAGTCGTCAATGGGAGGTTTCATAT TCATCATCACAGAACTTACTTGGTACGGGCTCACACCAGTCTATAACAGTTCTCTACTCA ATGGTTTCAACAAAGACTGCCACACCTTGCGTTGGCCCTCAAATTGTTACTATTGACTAT TTTTGGGACAGTGATATTTCCATTGGTCAGTTTATCGAGAACGTCGTGGGAACTGCCAGG TATCCCTGCCAACAAGGCTGTAATGGTCTGTATTTGGATCATTATAGAAGCTATGTTCAT GGGTCAGGAAAAGTAGACGTTTTAATCGAAAAATTCCAAACTAGATTACCAAAATTAAAA GATATTATCCTCACGTGGAGTTATTGTAAAAAATGTGGGACATCTACTCCGATTTTACAA ATTAGCGAAAAAACCTGGAATCATTCTTTCGGTAAATATTTAGAAGTCATGTTTTGGAGT TACAAAGATAGCGTGACAGGAATTGGGAAATGTCCTCATGATTTCACTAAAGATCATGTC AAATACTTTGGTTACAATGATTTAGTGGTTCGACTAGAATATTCTGATTTAGAAGTTCAT GAATTGATAACCCCACCACGCAAAATAAAATGGAAACCTCATATCGACATCAAACTGAAG GTGGAACTATACTATAAGATTCTGGAAAAGATCAATAATTTCTATGGGAGTGTCCTAAGT CGGTTGGAACGTATTAAATTGGATAGTATGACTAAAGATAAAGTTCTTAGTGGACAAGCT AAAATTATCGAATTGAAGAGCAATGCTACAGAGGAGCAAAAATTAATGTTGCAAGACCTG GATACTTTTTACGCAGATAGTCCTTGCGATCAACATTTACCATTGAACCTGGTTATCAAA TCTCTATACGATAAGGCTGTAAATTGGAACTCTACATTTGCGATATTTGCAAAAAGCTAT TTGCCTTCTGAAACTGATATTAGTCGTATCACGGCCAAGCAGCTGAAAAAACTGTTTTAC GATTCTTCAAGAAAAGACAGCGAAGACAAAAAATCACTTCATGATGAAAAGGCGAAGACG AGGAAACCAGAAAAGAACGAATTACCGTTAGAGGGATTGAAAGATGTCGAAAAGCCGAAA ATTGATAGCAAGAATACGACAGAGAATCGTGACAGAACGAACGAACCACAGAATGCCGTG ACGATAACCACTTTTAAAGACGACACTCCAATTATTCCAACAAGTGGTACTAGTCACTTG ACAGTTACACCTTCTGCATCGTCGGTTTCTTCTTCTTTGACCCCTCAAACCGAAGAACGT CCGCCAATAAGCAGAAGTGGAACAGGTATATCTATGACTCATGATAAAAGCACTAGACCT AACATAAGAAAAATGAGTAGTGATAGTAGTTTATGTGGACTAGCATCACTTGCTAATGAA TATAGTAAAAATAATAAGGTGAGTAAGCTAGCTACATTTTTCGATCAAATGCATTTTGAT GCCCTGTCGAAAGAATTTGAACTGGAACGTGAAAGGGAAAGATTACAACTTAATAAGGAT AAATACCAAGCCATACGATTACAAACATCCACACCAATTGTGGAAATTTATAAAAATGTC AAAGACGCAGTTGATGAACCGCTTCACTCTAGAAGTTCAGGCAATAATTTGTCATCTGCG AATGTTAAGACTTTGGAGGCACCTGTGGGAGAACATTCCCGGGCCAATAATTGTAATCCT CCTAATTTAGATCAAAACTTGGAAACTGAATTAGAAAATTCCATTAGCCAATGGGGAGAA AACATTTTGAACCCCTCTGGAAAGACCACCGCAAGTACTCATTTAAATTCTAAGCCAGTA GTAAAGGAAACTTCAGAAAATCCAAAGTCAATAGTTAGAGAATCAGATAATTCAAAGTCG GAGCCGCTTCCACCTGTAATAACAACAACAACTGTCAATAAGGTAGAATCCACTCCACAA CCTGAAAAATCATTATTGATGAAAACCTTGAGCAACTTTTGGGCAGATCGATCTGCGTAT TTGTGGAAACCCCTTGTTTATCCAACCTGTCCTTCGGAACACATTTTTACTGATAGTGAC GTTATAATCCGCGAAGATGAACCAAGTTCCTTGATCGCATTTTGCTTAAGTACTTCTGAC TATAGAAATAAAATGATGAACTTGAATGTCCAGCAACAACAGCAGCAGCAAACAGCAGAA GCTGCTCCTGCCAAAACAGGAGGAAACAGTGGAGGAACTACGCAAACCGGGGATCCTTCT GTTAACATTTCTCCCTCAGTTTCTACAACTTCTCATAATAAGGGGAGGGACTCAGAAATT TCATCCTTAGTAACCACCAAAGAGGGCTTGTTGAACACCCCTCCCATTGAAGGCGCCCGA GATAGGACCCCACAAGAATCACAAACGCATTCCCAAGCAAATTTAGATACTCTTCAAGAA TTAGAGAAAATTATGACAAAAAAAACAGCCACACACTTGAGATATCAATTTGAGGAGGGT TTAACGGTAATGTCATGTAAAATTTTCTTCACAGAACATTTTGACGTGTTTAGAAAGATT TGTGATTGTCAAGAAAACTTCATTCAAAGTTTGTCAAGATGTGTTAAATGGGATTCCAAT GGGGGGAAAAGTGGGAGCGGGTTTTTAAAGACTTTGGATGATAGATTTATTATAAAGGAG CTGTCACACGCAGAACTGGAAGCCTTCATAAAATTTGCACCAAGTTATTTTGAATACATG GCACAAGCAATGTTCCATGATTTGCCCACGACTTTGGCTAAAGTATTTGGGTTTTATCAA ATTCAAGTAAAAAGCTCTATTTCAAGTTCCAAAAGTTATAAAATGGACGTTATCATTATG GAGAACTTATTTTATGAGAAGAAAACAACAAGAATATTTGATTTGAAGGGTTCCATGAGA AATAGACATGTCGAACAAACAGGTAAAGCTAATGAAGTGTTGTTGGATGAAAATATGGTA GAGTATATTTACGAATCACCTATTCATGTTCGCGAATATGACAAGAAACTTTTAAGAGCT TCTGTTTGGAACGACACATTATTTTTGGCCAAAATGAATGTGATGGATTATTCCTTAGTC ATTGGTATTGACAATGAAGGGTATACTTTGACAGTAGGAATCATTGATTTCATTCGAACG TTTACATGGGACAAAAAACTAGAAAGTTGGGTCAAAGAGAAAGGCCTAGTAGGGGGGGCC AGTGTGATCAAGCAGCCTACCGTTGTTACACCAAGACAATATAAAAAACGTTTTAGGGAA GCAATGGAGAGGTATATTTTGATGGTTCCTGATCCGTGGTATTGGGAAGGAAATTAA

Protein Properties

Pfam Domain Function

Cpn60_TCP1 (PF00118 )
FYVE (PF01363 )
PIP5K (PF01504 )

Protein Residues

2278

Protein Molecular Weight

257447.0

Protein Theoretical pI

8.51

Signalling Regions

None

Transmembrane Regions

None

Protein Sequence

>1-phosphatidylinositol-3-phosphate 5-kinase FAB1 MSSEEPHASISFPDGSHVRSSSTGTSSVNTIDATLSRPNYIKKPSLHIMSTSTTSTTTDL VTNPILSNISVPKISPPTSSSIATATSTSHVTGTASHSNIKANANTSTSVNKKNLPPTTS GRIPSSTIKRYPSRYKPSHSLQLPIKNDSNFKRSSIYASKSTVTAIPIRNNRPISMQNSY ARTPDSDHDDVGDEVSSIKSASSSLTASLSKSFLFAFYNNRKKDKTSNNGVLSKEYWMKD ESSKECFSCGKTFNTFRRKHHCRICGQIFCSSCTLLIDGDRFGCHAKMRVCYNCYEHADT YEDSSDEENDSTMQLNEPRSRSRSRSSNTNPYSHSHSHLHLISQDNHNGTDLHDPVAATD NPQQQNEVYLLNDDDVQSIMTSGEDSKLFISTPPPPPKMAIPATKQGGSLEISFDSENDR ALHYQDDNPGRHHHLDSVPTRYTIRDMDNISHYDTNSNSTLRPHYNTNNSTITINNLNNT TSNNSNYNNTNSNSNINNPAHSLRRSIFHYVSSNSVNKDSNNSSATPASSAQSSSILDPA NRIIGNYAHRNYKFKFNYNSKGPSQQNDTANGNNDNNNNNNNNNNNNNNNSASGIADNNN IPSNDNGTTFTLDKKKRNPLTKSKSTSAYLEYPLNEEDSSEDEGSMSIYSVLNDDHKTDN PIRSMRNSTKSFQRAQASLQRMRFRRKSKSKHFPNNSKSSIYRDLNFLTNSTPNLLSVVS DDNLYDDSSPLQDKASSSAASRLTDRKFSNSSGSNNNSNSNSNINTDPWKRIASISGFKL KKEKKRELNEVSLLHMHALLKQLLNDQEISNLQEWITLLDGALRKVLRTILNARDLNTLD FRQTYVKIKRISGGSPQNSEYIDGVVFSKALPSKTMPRHLKNPRILLIMFPLEYQKNNNH FLSIESVFRQEREYLDKLVSRLKSLHPDIIYVGANVSGYALELLNDSGIVVQFNMKPQVI ERIAKLTEADIAISVDKLATNIKMGECETFEVKSYIYGNISKTYTFLRGCNPELGGTILL RGDSLENLRKIKQVSEFMVYAIFSLKLESSFFNDNFIQLSTDVYLKRAESKKLQVFEGYF ADFLIKFNNRILTVSPTVDFPIPFLLEKARGLEKKLIERINQYESESDLDRQTQLNMLQG LESTITKKHLGNLIKFLHEMEIENLELEFQKRSRQWEVSYSSSQNLLGTGSHQSITVLYS MVSTKTATPCVGPQIVTIDYFWDSDISIGQFIENVVGTARYPCQQGCNGLYLDHYRSYVH GSGKVDVLIEKFQTRLPKLKDIILTWSYCKKCGTSTPILQISEKTWNHSFGKYLEVMFWS YKDSVTGIGKCPHDFTKDHVKYFGYNDLVVRLEYSDLEVHELITPPRKIKWKPHIDIKLK VELYYKILEKINNFYGSVLSRLERIKLDSMTKDKVLSGQAKIIELKSNATEEQKLMLQDL DTFYADSPCDQHLPLNLVIKSLYDKAVNWNSTFAIFAKSYLPSETDISRITAKQLKKLFY DSSRKDSEDKKSLHDEKAKTRKPEKNELPLEGLKDVEKPKIDSKNTTENRDRTNEPQNAV TITTFKDDTPIIPTSGTSHLTVTPSASSVSSSLTPQTEERPPISRSGTGISMTHDKSTRP NIRKMSSDSSLCGLASLANEYSKNNKVSKLATFFDQMHFDALSKEFELERERERLQLNKD KYQAIRLQTSTPIVEIYKNVKDAVDEPLHSRSSGNNLSSANVKTLEAPVGEHSRANNCNP PNLDQNLETELENSISQWGENILNPSGKTTASTHLNSKPVVKETSENPKSIVRESDNSKS EPLPPVITTTTVNKVESTPQPEKSLLMKTLSNFWADRSAYLWKPLVYPTCPSEHIFTDSD VIIREDEPSSLIAFCLSTSDYRNKMMNLNVQQQQQQQTAEAAPAKTGGNSGGTTQTGDPS VNISPSVSTTSHNKGRDSEISSLVTTKEGLLNTPPIEGARDRTPQESQTHSQANLDTLQE LEKIMTKKTATHLRYQFEEGLTVMSCKIFFTEHFDVFRKICDCQENFIQSLSRCVKWDSN GGKSGSGFLKTLDDRFIIKELSHAELEAFIKFAPSYFEYMAQAMFHDLPTTLAKVFGFYQ IQVKSSISSSKSYKMDVIIMENLFYEKKTTRIFDLKGSMRNRHVEQTGKANEVLLDENMV EYIYESPIHVREYDKKLLRASVWNDTLFLAKMNVMDYSLVIGIDNEGYTLTVGIIDFIRT FTWDKKLESWVKEKGLVGGASVIKQPTVVTPRQYKKRFREAMERYILMVPDPWYWEGN

References

External Links

Resource	Link
Saccharomyces Genome Database	FAB1
Uniprot ID	P34756
Uniprot Name	FAB1_YEAST
GenBank Gene ID	D50617
Genebank Protein ID	836774

General Reference

Yamamoto, A., DeWald, D. B., Boronenkov, I. V., Anderson, R. A., Emr, S. D., Koshland, D. (1995). "Novel PI(4)P 5-kinase homologue, Fab1p, essential for normal vacuole function and morphology in yeast." Mol Biol Cell 6:525-539.7663021
Murakami, Y., Naitou, M., Hagiwara, H., Shibata, T., Ozawa, M., Sasanuma, S., Sasanuma, M., Tsuchiya, Y., Soeda, E., Yokoyama, K., et, a. l. .. (1995). "Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Nat Genet 10:261-268.7670463
Cooke, F. T., Dove, S. K., McEwen, R. K., Painter, G., Holmes, A. B., Hall, M. N., Michell, R. H., Parker, P. J. (1998). "The stress-activated phosphatidylinositol 3-phosphate 5-kinase Fab1p is essential for vacuole function in S. cerevisiae." Curr Biol 8:1219-1222.9811604
Bonangelino, C. J., Nau, J. J., Duex, J. E., Brinkman, M., Wurmser, A. E., Gary, J. D., Emr, S. D., Weisman, L. S. (2002). "Osmotic stress-induced increase of phosphatidylinositol 3,5-bisphosphate requires Vac14p, an activator of the lipid kinase Fab1p." J Cell Biol 156:1015-1028.11889142
Gary, J. D., Sato, T. K., Stefan, C. J., Bonangelino, C. J., Weisman, L. S., Emr, S. D. (2002). "Regulation of Fab1 phosphatidylinositol 3-phosphate 5-kinase pathway by Vac7 protein and Fig4, a polyphosphoinositide phosphatase family member." Mol Biol Cell 13:1238-1251.11950935
Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
Gruhler, A., Olsen, J. V., Mohammed, S., Mortensen, P., Faergeman, N. J., Mann, M., Jensen, O. N. (2005). "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Mol Cell Proteomics 4:310-327.15665377
Duex, J. E., Tang, F., Weisman, L. S. (2006). "The Vac14p-Fig4p complex acts independently of Vac7p and couples PI3,5P2 synthesis and turnover." J Cell Biol 172:693-704.16492811
Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
Jin, N., Chow, C. Y., Liu, L., Zolov, S. N., Bronson, R., Davisson, M., Petersen, J. L., Zhang, Y., Park, S., Duex, J. E., Goldowitz, D., Meisler, M. H., Weisman, L. S. (2008). "VAC14 nucleates a protein complex essential for the acute interconversion of PI3P and PI(3,5)P(2) in yeast and mouse." EMBO J 27:3221-3234.19037259
Botelho, R. J., Efe, J. A., Teis, D., Emr, S. D. (2008). "Assembly of a Fab1 phosphoinositide kinase signaling complex requires the Fig4 phosphoinositide phosphatase." Mol Biol Cell 19:4273-4286.18653468
Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956