Glutaminyl-tRNA synthetase (P13188)

Identification
NameGlutaminyl-tRNA synthetase
Synonyms
  • Glutamine--tRNA ligase
  • GlnRS
Gene NameGLN4
Enzyme Class
Biological Properties
General FunctionInvolved in nucleotide binding
Specific FunctionATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln)
Cellular LocationNot Available
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG Reactions
Adenosine triphosphate + L-Glutamine + tRNA(Gln) → Adenosine monophosphate + Pyrophosphate + Gln-tRNA(Gln)
Metabolites
YMDB IDNameView
YMDB00002L-GlutamineShow
YMDB00097Adenosine monophosphateShow
YMDB00109Adenosine triphosphateShow
YMDB00219PyrophosphateShow
GO Classification
Component
cell part
intracellular part
cytoplasm
Function
glutamine-tRNA ligase activity
nucleoside binding
purine nucleoside binding
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
ligase activity, forming carbon-oxygen bonds
ligase activity, forming aminoacyl-tRNA and related compounds
catalytic activity
aminoacyl-tRNA ligase activity
nucleotide binding
ligase activity
binding
Process
tRNA aminoacylation for protein translation
macromolecule biosynthetic process
cellular macromolecule biosynthetic process
translation
metabolic process
macromolecule metabolic process
glutaminyl-tRNA aminoacylation
cellular macromolecule metabolic process
RNA metabolic process
biosynthetic process
ncRNA metabolic process
tRNA metabolic process
tRNA aminoacylation
Gene Properties
Chromosome Locationchromosome 15
LocusYOR168W
Gene Sequence>2430 bp ATGTCTTCTGTAGAAGAATTGACTCAGCTGTTTTCACAGGTTGGGTTTGAAGATAAAAAG GTGAAGGAGATTGTCAAGAATAAAAAGGTTTCTGATTCACTTTACAAATTGATTAAGGAG ACTCCTTCGGACTATCAATGGAACAAATCTACTAGAGCGCTAGTACACAACCTGGCATCT TTTGTCAAAGGTACAGATCTACCCAAGTCTGAATTAATTGTTAACGGCATCATTAATGGT GATTTGAAGACATCTCTGCAAGTCGATGCCGCATTTAAATATGTAAAGGCTAATGGCGAG GCATCCACCAAAATGGGTATGAATGAAAACTCTGGTGTTGGTATTGAGATTACAGAGGAT CAGGTTAGAAATTACGTCATGCAGTACATTCAAGAAAACAAGGAAAGAATTTTAACTGAA CGCTATAAACTGGTGCCAGGTATTTTTGCCGATGTTAAAAACTTAAAGGAGCTGAAGTGG GCCGATCCACGTAGTTTCAAGCCAATTATCGACCAAGAAGTCTTAAAACTTTTGGGTCCA AAGGATGAGAGAGACTTAATCAAGAAGAAGACCAAGAATAATGAAAAGAAGAAAACCAAT TCTGCCAAGAAGAGTTCTGATAATTCTGCATCATCCGGTCCAAAGAGGACTATGTTCAAT GAAGGTTTCCTAGGTGATTTGCATAAAGTGGGTGAAAACCCACAAGCTTACCCAGAATTA ATGAAGGAACACCTTGAGGTTACTGGTGGCAAAGTTCGTACAAGGTTCCCTCCGGAGCCC AATGGATATTTGCATATTGGTCATTCTAAAGCTATTATGGTTAATTTTGGCTATGCTAAA TATCACAATGGTACCTGTTATTTAAGATTTGACGATACCAACCCCGAAAAGGAAGCTCCT GAATATTTTGAATCCATTAAGAGAATGGTTTCTTGGTTAGGTTTTAAACCATGGAAAATT ACTTACTCAAGTGATTATTTTGACGAGCTTTATCGCCTCGCTGAAGTGTTGATCAAAAAC GGCAAAGCTTACGTTTGTCATTGTACCGCCGAAGAAATTAAAAGAGGTCGTGGTATTAAA GAAGACGGTACTCCAGGTGGGGAGAGATATGCTTGTAAGCATCGTGATCAGTCGATAGAA CAAAACCTGCAAGAATTCAGAGATATGAGAGATGGCAAGTACAAACCTGGTGAAGCTATC TTAAGAATGAAACAGGATCTAAATTCACCAAGCCCACAAATGTGGGACTTGATTGCGTAT AGAGTTTTAAATGCTCCACATCCAAGAACGGGAACAAAATGGAGAATTTATCCTACTTAC GATTTTACACATTGTTTGGTCGATTCAATGGAAAATATTACACATTCTTTATGTACTACT GAATTCTACCTATCTAGAGAAAGCTATGAATGGTTATGTGATCAGGTTCATGTGTTCAGA CCCGCTCAAAGAGAATATGGTCGTTTGAATATCACCGGTACAGTTTTATCCAAAAGAAAA ATTGCGCAATTGGTGGATGAAAAATTCGTTAGAGGCTGGGACGATCCTAGACTATTCACG CTTGAAGCTATACGCAGACGTGGTGTTCCTCCTGGTGCCATTTTATCCTTTATCAACACA TTAGGTGTTACCACAAGTACCACAAACATTCAAGTTGTGAGGTTTGAGAGCGCTGTTAGA AAGTACCTAGAAGATACCACACCGAGATTAATGTTTGTGTTGGATCCTGTTGAAGTTGTT GTCGATAATTTATCTGACGATTATGAAGAATTGGCAACTATTCCTTACAGACCTGGTACT CCAGAATTTGGTGAAAGAACTGTACCATTTACAAACAAATTCTACATTGAAAGGTCAGAT TTCTCAGAAAACGTTGATGATAAGGAATTTTTCAGATTAACACCAAACCAACCAGTTGGT TTAATTAAAGTCTCACACACAGTTTCTTTCAAAAGTTTGGAAAAAGATGAAGCTGGTAAA ATTATAAGAATTCATGTCAACTATGATAACAAGGTTGAAGAAGGTTCGAAACCAAAGAAA CCAAAAACTTATATTCAATGGGTTCCTATCTCTTCAAAATATAATTCTCCATTGAGAGTT ACTGAAACTAGGGTTTACAACCAATTGTTCAAATCTGAAAATCCATCTTCTCATCCTGAA GGCTTCTTGAAGGACATCAACCCAGAAAGTGAAGTCGTTTATAAAGAATCTGTCATGGAA CATAACTTCGGGGACGTTGTCAAGAACAGTCCATGGGTCGTTGATTCTGTTAAAAACTCG GAATTTTATGTTGAAGAAGATAAGGATTCCAAAGAAGTGTGTAGATTCCAAGCTATGAGA GTAGGTTATTTCACTCTGGATAAAGAGAGTACGACTTCTAAGGTTATTTTGAATAGAATA GTTAGCTTGAAGGACGCAACTTCCAAGTGA
Protein Properties
Pfam Domain Function
Protein Residues809
Protein Molecular Weight93132.20313
Protein Theoretical pI8.67
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Glutaminyl-tRNA synthetase MSSVEELTQLFSQVGFEDKKVKEIVKNKKVSDSLYKLIKETPSDYQWNKSTRALVHNLAS FVKGTDLPKSELIVNGIINGDLKTSLQVDAAFKYVKANGEASTKMGMNENSGVGIEITED QVRNYVMQYIQENKERILTERYKLVPGIFADVKNLKELKWADPRSFKPIIDQEVLKLLGP KDERDLIKKKTKNNEKKKTNSAKKSSDNSASSGPKRTMFNEGFLGDLHKVGENPQAYPEL MKEHLEVTGGKVRTRFPPEPNGYLHIGHSKAIMVNFGYAKYHNGTCYLRFDDTNPEKEAP EYFESIKRMVSWLGFKPWKITYSSDYFDELYRLAEVLIKNGKAYVCHCTAEEIKRGRGIK EDGTPGGERYACKHRDQSIEQNLQEFRDMRDGKYKPGEAILRMKQDLNSPSPQMWDLIAY RVLNAPHPRTGTKWRIYPTYDFTHCLVDSMENITHSLCTTEFYLSRESYEWLCDQVHVFR PAQREYGRLNITGTVLSKRKIAQLVDEKFVRGWDDPRLFTLEAIRRRGVPPGAILSFINT LGVTTSTTNIQVVRFESAVRKYLEDTTPRLMFVLDPVEVVVDNLSDDYEELATIPYRPGT PEFGERTVPFTNKFYIERSDFSENVDDKEFFRLTPNQPVGLIKVSHTVSFKSLEKDEAGK IIRIHVNYDNKVEEGSKPKKPKTYIQWVPISSKYNSPLRVTETRVYNQLFKSENPSSHPE GFLKDINPESEVVYKESVMEHNFGDVVKNSPWVVDSVKNSEFYVEEDKDSKEVCRFQAMR VGYFTLDKESTTSKVILNRIVSLKDATSK
References
External Links
ResourceLink
Saccharomyces Genome Database GLN4
Uniprot IDP13188
Uniprot NameSYQ_YEAST
GenBank Gene IDU55021
Genebank Protein ID1293729
General Reference
  • Ludmerer, S. W., Schimmel, P. (1987). "Gene for yeast glutamine tRNA synthetase encodes a large amino-terminal extension and provides a strong confirmation of the signature sequence for a group of the aminoacyl-tRNA synthetases." J Biol Chem 262:10801-10806.3301841
  • Madania, A., Poch, O., Tarassov, I., Winsor, B., Martin, R. (1996). "Analysis of a 22,956 bp region on the right arm of Saccharomyces cerevisiae chromosome XV." Yeast 12:1563-1573.8972579
  • Dujon, B., Albermann, K., Aldea, M., Alexandraki, D., Ansorge, W., Arino, J., Benes, V., Bohn, C., Bolotin-Fukuhara, M., Bordonne, R., Boyer, J., Camasses, A., Casamayor, A., Casas, C., Cheret, G., Cziepluch, C., Daignan-Fornier, B., Dang, D. V., de Haan, M., Delius, H., Durand, P., Fairhead, C., Feldmann, H., Gaillon, L., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Nature 387:98-102.9169874
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356