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Histidyl-tRNA synthetase, mitochondrial (P07263)

Identification
NameHistidyl-tRNA synthetase, mitochondrial
Synonyms
  • Histidine--tRNA ligase
  • HisRS
Gene NameHTS1
Enzyme Class
Biological Properties
General FunctionInvolved in nucleotide binding
Specific FunctionATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His)
Cellular LocationMitochondrion. Cytoplasm
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG Reactions
Adenosine triphosphate + L-Histidine + tRNA(His) → Adenosine monophosphate + Pyrophosphate + His-tRNA(His)
Metabolites
YMDB IDNameView
YMDB00097Adenosine monophosphateShow
YMDB00109Adenosine triphosphateShow
YMDB00219PyrophosphateShow
YMDB00369L-HistidineShow
GO Classification
Component
intracellular part
cytoplasm
cell part
Function
binding
nucleoside binding
purine nucleoside binding
histidine-tRNA ligase activity
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
ligase activity, forming carbon-oxygen bonds
ligase activity, forming aminoacyl-tRNA and related compounds
catalytic activity
aminoacyl-tRNA ligase activity
nucleotide binding
ligase activity
Process
tRNA aminoacylation
tRNA aminoacylation for protein translation
macromolecule biosynthetic process
cellular macromolecule biosynthetic process
translation
histidyl-tRNA aminoacylation
metabolic process
macromolecule metabolic process
cellular macromolecule metabolic process
RNA metabolic process
ncRNA metabolic process
tRNA metabolic process
biosynthetic process
Gene Properties
Chromosome Locationchromosome 16
LocusYPR033C
Gene Sequence>1641 bp ATGCTTAGTAGATCACTAAATAAAGTAGTCACATCTATAAAGTCGTCTTCGATAATCAGG ATGTCATCCGCTACCGCTGCTGCTACTTCTGCTCCTACTGCTAATGCTGCTAATGCTCTA AAAGCTTCAAAGGCTCCAAAGAAAGGTAAACTACAAGTGTCTTTGAAAACACCTAAGGGT ACGAAGGATTGGGCTGATAGTGATATGGTTATCAGAGAAGCCATTTTTAGCACTTTATCT GGCCTATTCAAGAAGCATGGTGGTGTCACTATCGACACACCTGTTTTCGAGTTAAGAGAA ATTTTAGCAGGCAAGTATGGTGAGGACTCCAAACTGATCTACAACTTGGAGGACCAGGGT GGTGAATTATGTTCTTTACGTTACGATTTGACCGTTCCTTTTGCCAGGTACGTTGCCATG AACAATATTCAGAGTATCAAGAGGTATCACATTGCAAAAGTCTACAGAAGAGACCAACCA GCCATGACTAAGGGTCGTATGAGAGAATTTTACCAGTGTGATTTTGACGTTGCAGGCACA TTCGAATCTATGGTTCCTGATTCCGAATGTTTGTCCATTTTAGTGGAAGGTTTGACCAGT TTGGGAATCAAAGATTTCAAAATCAAATTGAATCACAGGAAGATTCTTGATGGGATCTTC CAAATCGCTGGTGTTAAAGATGAGGATGTGAGGAAAATCTCTTCTGCTGTCGATAAGTTG GACAAATCTCCATGGGAAGCAGTCAAGAAGGAAATGACCGAAGAAAAAGGTCAATCCGAA GAAACTGCTGATAAAATCGGTGAGTATGTTAAATTAAATGGTTCTTTGAAGGAAATTCAC GCTGTTCTATCCGCAGATGCCAACATTACTTCAAACGAAAAGGCTAAACAAGGTTTGGAT GATATTGCTACTTTGATGAAGTATACCGAAGCATTTGACATTGACTCATTCATCTCTTTT GATCTTTCTCTTGCAAGAGGTTTGGATTACTATACAGGTTTGATCTATGAAGTTGTTACT TCTGCATCTGCTCCACCAGAGAATGCTTCAGAATTGAAGAAGAAGGCCAAATCTGCAGAA GACGCGTCAGAATTCGTTGGTGTCGGCTCTATTGCAGCCGGTGGTCGATATGATAATTTG GTCAACATGTTCTCTGAAGCTTCCGGCAAAAAATCTACTCAAATTCCATGTGTTGGTATT TCATTTGGTGTTGAAAGAATATTTTCTTTGATCAAACAAAGAATTAATTCCTCTACAACT ATAAAGCCAACGGCTACACAGGTGTTCGTTATGGCTTTTGGTGGTGGTAAGGACTGGACT GGTTATTTACCCGAAAGAATGAAGGTTACTAAGCAATTATGGGATGCTGGCATTGAAGCT GAATATGTCTATAAGGCCAAGGCTAATCCTAGGAAGCAATTCGATGCTGCTGAAAAAGCG GGTTGTCACATTGCCGTAATCCTCGGTAAGGAGGAGTACTTAGAAGGTAAATTACGTGTC AAAAGATTGGGCCAAGAATTTGCCGATGATGATGGTGAATTGGTTAGTGCGGCTGACATT GTTCCTATTGTTCAAGAAAAGTTATCACAAATTCATGAAGATGGTCTAAATGAGGTCACT CGTTTAATTAAAGGATTATAA
Protein Properties
Pfam Domain Function
Protein Residues546
Protein Molecular Weight59952.0
Protein Theoretical pI7.87
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Histidyl-tRNA synthetase, mitochondrial MLSRSLNKVVTSIKSSSIIRMSSATAAATSAPTANAANALKASKAPKKGKLQVSLKTPKG TKDWADSDMVIREAIFSTLSGLFKKHGGVTIDTPVFELREILAGKYGEDSKLIYNLEDQG GELCSLRYDLTVPFARYVAMNNIQSIKRYHIAKVYRRDQPAMTKGRMREFYQCDFDVAGT FESMVPDSECLSILVEGLTSLGIKDFKIKLNHRKILDGIFQIAGVKDEDVRKISSAVDKL DKSPWEAVKKEMTEEKGQSEETADKIGEYVKLNGSLKEIHAVLSADANITSNEKAKQGLD DIATLMKYTEAFDIDSFISFDLSLARGLDYYTGLIYEVVTSASAPPENASELKKKAKSAE DASEFVGVGSIAAGGRYDNLVNMFSEASGKKSTQIPCVGISFGVERIFSLIKQRINSSTT IKPTATQVFVMAFGGGKDWTGYLPERMKVTKQLWDAGIEAEYVYKAKANPRKQFDAAEKA GCHIAVILGKEEYLEGKLRVKRLGQEFADDDGELVSAADIVPIVQEKLSQIHEDGLNEVT RLIKGL
References
External Links
ResourceLink
Saccharomyces Genome Database HTS1
Uniprot IDP07263
Uniprot NameSYH_YEAST
GenBank Gene IDAY692751
Genebank Protein ID51012953
General Reference
  • Natsoulis, G., Hilger, F., Fink, G. R. (1986). "The HTS1 gene encodes both the cytoplasmic and mitochondrial histidine tRNA synthetases of S. cerevisiae." Cell 46:235-243.3521891
  • Bussey, H., Storms, R. K., Ahmed, A., Albermann, K., Allen, E., Ansorge, W., Araujo, R., Aparicio, A., Barrell, B., Badcock, K., Benes, V., Botstein, D., Bowman, S., Bruckner, M., Carpenter, J., Cherry, J. M., Chung, E., Churcher, C., Coster, F., Davis, K., Davis, R. W., Dietrich, F. S., Delius, H., DiPaolo, T., Hani, J., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Nature 387:103-105.9169875
  • Hu, Y., Rolfs, A., Bhullar, B., Murthy, T. V., Zhu, C., Berger, M. F., Camargo, A. A., Kelley, F., McCarron, S., Jepson, D., Richardson, A., Raphael, J., Moreira, D., Taycher, E., Zuo, D., Mohr, S., Kane, M. F., Williamson, J., Simpson, A., Bulyk, M. L., Harlow, E., Marsischky, G., Kolodner, R. D., LaBaer, J. (2007). "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Genome Res 17:536-543.17322287
  • Chiu, M. I., Mason, T. L., Fink, G. R. (1992). "HTS1 encodes both the cytoplasmic and mitochondrial histidyl-tRNA synthetase of Saccharomyces cerevisiae: mutations alter the specificity of compartmentation." Genetics 132:987-1001.1459448
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106