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Identification |
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Name | 3-isopropylmalate dehydrogenase |
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Synonyms | - 3-IPM-DH
- IMDH
- Beta-IPM dehydrogenase
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Gene Name | LEU2 |
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Enzyme Class | |
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Biological Properties |
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General Function | Involved in magnesium ion binding |
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Specific Function | Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate |
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Cellular Location | Cytoplasm |
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SMPDB Pathways | Not Available |
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KEGG Pathways | Valine, leucine and isoleucine biosynthesis | ec00290 | |
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SMPDB Reactions | Not Available |
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KEGG Reactions | |
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Metabolites | YMDB ID | Name | View |
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YMDB00110 | NAD | Show | YMDB00143 | NADH | Show | YMDB00368 | 3-Isopropylmalate | Show | YMDB00388 | Ketoleucine | Show | YMDB00412 | NAD(+) | Show | YMDB00449 | (2S)-2-Isopropyl-3-oxosuccinate | Show | YMDB00862 | hydron | Show | YMDB00912 | Carbon dioxide | Show |
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GO Classification | Component |
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intracellular part | cytoplasm | cell part | Function |
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3-isopropylmalate dehydrogenase activity | binding | nucleotide binding | ion binding | cation binding | metal ion binding | oxidoreductase activity | magnesium ion binding | catalytic activity | oxidoreductase activity, acting on CH-OH group of donors | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor | NAD or NADH binding | Process |
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cellular metabolic process | branched chain family amino acid metabolic process | leucine metabolic process | cellular amino acid and derivative metabolic process | leucine biosynthetic process | cellular amino acid metabolic process | oxidation reduction | metabolic process |
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Gene Properties |
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Chromosome Location | chromosome 3 |
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Locus | YCL018W |
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Gene Sequence | >1095 bp
ATGTCTGCCCCTAAGAAGATCGTCGTTTTGCCAGGTGACCACGTTGGTCAAGAAATCACA
GCCGAAGCCATTAAGGTTCTTAAAGCTATTTCTGATGTTCGTTCCAATGTCAAGTTCGAT
TTCGAAAATCATTTAATTGGTGGTGCTGCTATCGATGCTACAGGTGTCCCACTTCCAGAT
GAGGCGCTGGAAGCCTCCAAGAAGGTTGATGCCGTTTTGTTAGGTGCTGTGGGTGGTCCT
AAATGGGGTACCGGTAGTGTTAGACCTGAACAAGGTTTACTAAAAATCCGTAAAGAACTT
CAATTGTACGCCAACTTAAGACCATGTAACTTTGCATCCGACTCTCTTTTAGACTTATCT
CCAATCAAGCCACAATTTGCTAAAGGTACTGACTTCGTTGTTGTCAGAGAATTAGTGGGA
GGTATTTACTTTGGTAAGAGAAAGGAAGACGATGGTGATGGTGTCGCTTGGGATAGTGAA
CAATACACCGTTCCAGAAGTGCAAAGAATCACAAGAATGGCCGCTTTCATGGCCCTACAA
CATGAGCCACCATTGCCTATTTGGTCCTTGGATAAAGCTAATGTTTTGGCCTCTTCAAGA
TTATGGAGAAAAACTGTGGAGGAAACCATCAAGAACGAATTCCCTACATTGAAGGTTCAA
CATCAATTGATTGATTCTGCCGCCATGATCCTAGTTAAGAACCCAACCCACCTAAATGGT
ATTATAATCACCAGCAACATGTTTGGTGATATCATCTCCGATGAAGCCTCCGTTATCCCA
GGTTCCTTGGGTTTGTTGCCATCTGCGTCCTTGGCCTCTTTGCCAGACAAGAACACCGCA
TTTGGTTTGTACGAACCATGCCACGGTTCTGCTCCAGATTTGCCAAAGAATAAGGTCAAC
CCTATCGCCACTATCTTGTCTGCTGCAATGATGTTGAAATTGTCATTGAACTTGCCTGAA
GAAGGTAAGGCCATTGAAGATGCAGTTAAAAAGGTTTTGGATGCAGGTATCAGAACTGGT
GATTTAGGTGGTTCCAACAGTACCACGGAAGTCGGTGATGCTGTCGCCGAAGAAGTTAAG
AAAATCCTTGCTTAA |
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Protein Properties |
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Pfam Domain Function | |
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Protein Residues | 364 |
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Protein Molecular Weight | 38952.5 |
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Protein Theoretical pI | 5.48 |
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Signalling Regions | |
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Transmembrane Regions | |
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Protein Sequence | >3-isopropylmalate dehydrogenase
MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHLIGGAAIDATGVPLPD
EALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYANLRPCNFASDSLLDLS
PIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYTVPEVQRITRMAAFMALQ
HEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQLIDSAAMILVKNPTHLNG
IIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAFGLYEPCHGSAPDLPKNKVN
PIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTGDLGGSNSTTEVGDAVAEEVK
KILA |
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References |
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External Links | |
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General Reference | - Warmington, J. R., Anwar, R., Newlon, C. S., Waring, R. B., Davies, R. W., Indge, K. J., Oliver, S. G. (1986). "A 'hot-spot' for Ty transposition on the left arm of yeast chromosome III." Nucleic Acids Res 14:3475-3485.3010239
- Andreadis, A., Hsu, Y. P., Hermodson, M., Kohlhaw, G., Schimmel, P. (1984). "Yeast LEU2. Repression of mRNA levels by leucine and primary structure of the gene product." J Biol Chem 259:8059-8062.6330094
- Oliver, S. G., van der Aart, Q. J., Agostoni-Carbone, M. L., Aigle, M., Alberghina, L., Alexandraki, D., Antoine, G., Anwar, R., Ballesta, J. P., Benit, P., et, a. l. .. (1992). "The complete DNA sequence of yeast chromosome III." Nature 357:38-46.1574125
- Froman, B. E., Tait, R. C., Rodriguez, R. L. (1984). "Nucleotide sequence of the 3' terminal region of the LEU2 gene from Saccharomyces cerevisiae." Gene 31:257-261.6396161
- Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956
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