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Identification
NameAsparagine synthetase [glutamine-hydrolyzing] 2
Synonyms
  • Glutamine-dependent asparagine synthetase 2
Gene NameASN2
Enzyme Class
Biological Properties
General FunctionInvolved in asparagine synthase (glutamine-hydrolyzing) activity
Specific FunctionATP + L-aspartate + L-glutamine + H(2)O = AMP + diphosphate + L-asparagine + L-glutamate
Cellular LocationNot Available
SMPDB Pathways
Asparagine metabolismPW002274 ThumbThumb?image type=greyscaleThumb?image type=simple
Nitrogen metabolismPW002504 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Alanine, aspartate and glutamate metabolismec00250 Map00250
Nitrogen metabolismec00910 Map00910
SMPDB Reactions
L-Aspartic acid + Adenosine triphosphate + AmmoniumL-Asparagine + Adenosine monophosphate + Pyrophosphate + hydron
KEGG Reactions
L-Aspartic acid + Adenosine triphosphate + water + L-GlutamineAdenosine monophosphate + Pyrophosphate + L-Asparagine + L-Glutamic acid + hydron
Metabolites
YMDB IDNameView
YMDB00002L-GlutamineShow
YMDB00097Adenosine monophosphateShow
YMDB00109Adenosine triphosphateShow
YMDB00219PyrophosphateShow
YMDB00226L-AsparagineShow
YMDB00271L-Glutamic acidShow
YMDB00423AmmoniumShow
YMDB00862hydronShow
YMDB00890waterShow
YMDB00896L-Aspartic acidShow
GO Classification
Component
Not Available
Function
catalytic activity
ligase activity
ligase activity, forming carbon-nitrogen bonds
carbon-nitrogen ligase activity, with glutamine as amido-N-donor
asparagine synthase (glutamine-hydrolyzing) activity
Process
aspartate family amino acid metabolic process
asparagine metabolic process
metabolic process
asparagine biosynthetic process
cellular metabolic process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
Gene Properties
Chromosome Locationchromosome 7
LocusYGR124W
Gene Sequence>1719 bp ATGTGTGGTATCTTTGCAGCCTTCAAGCATGAAGATATTCACAACTTCAAACCAAAAGCT CTACAACTATCTAAAAAAATCAGACACCGTGGCCCAGATTGGTCAGGAAATGCCGTTATG AATTCCACCATTTTCGTTCACGAGAGGTTGGCTATTGTTGGTTTAGACTCCGGTGCCCAG CCAATCACTTCAGCTGATGGCGAATATATGCTTGGCGTTAATGGTGAGATCTACAACCAC ATCCAACTAAGGGAGATGTGCTCTGATTACAAGTTTCAAACTTTCAGTGACTGTGAACCC ATCATACCGTTATATTTGGAACATGATATCGATGCTCCAAAATATCTGGACGGTATGTTC GCATTTTGTCTGTATGATTCCAAGAAAGACCGTATTGTCGCTGCAAGAGACCCTATCGGT GTTGTCACTTTATACATGGGGCGTTCTTCTCAGTCTCCAGAGACCGTTTATTTTGCCTCC GAATTAAAATGTCTAACTGACGTTTGTGACAGTATCATTTCGTTCCCTCCTGGTCATGTC TACGATTCTGAAACGGACAAGATTACTCGTTACTTTACCCCAGACTGGTTGGATGAAAAG CGTATCCCATCCACCCCAGTTGACTACCATGCTATCAGACACAGTTTAGAAAAGGCCGTT AGAAAGAGGCTAATGGCTGAAGTTCCATACGGTGTTCTTCTATCCGGTGGGCTGGACTCT TCTTTGATTGCTGCGATTGCTGCTCGTGAAACGGAAAAAGCTAATGCTGATGCTAACGAA GACAACAATGTTGACGAGAAGCAACTTGCAGGTATCGATGACCAAGGCCATCTACACACA TCCGGTTGGTCTCGTTTGCATTCGTTTGCGATTGGTCTACCAAATGCACCTGATTTACAA GCGGCTAGAAAAGTCGCCAAATTCATTGGTTCTATCCACCATGAACACACTTTTACATTA CAAGAAGGTTTGGATGCTTTGGACGACGTGATCTACCATTTGGAAACTTACGACGTTACC ACTATCAGAGCTTCTACACCAATGTTCTTACTATCTAGAAAGATTAAGGCCCAAGGTGTC AAAATGGTTCTTTCTGGTGAAGGCTCGGACGAAATATTCGGTGGCTATCTATATTTCGCA CAAGCACCTTCTGCTGCAGAATTTCACACCGAATCTGTGCAACGTGTCAAGAACTTGCAT TTGGCAGATTGTTTGAGAGCTAATAAGTCCACGATGGCTTGGGGTCTAGAAGCTCGTGTT CCCTTCTTAGACAAAGACTTTTTGCAGCTATGTATGAACATTGATCCAAATGAAAAGATG ATCAAGCCAAAGGAAGGACGTATCGAAAAATACATTTTAAGAAAGGCATTCGACACTACA GATGAACCAGATGTTAAGCCATACCTACCTGAAGAAATCTTATGGAGACAAAAGGAACAA TTTTCCGATGGTGTTGGCTACTCATGGATTGACGGCCTAAGAGACACTGCTGAAAGGGCC ATTTCTGACGCCATGTTTGCCAATCCAAAGGCTGATTGGGGCGACGATATTCCAACCACC AAAGAAGCTTACTGGTACAGGCTGAAGTTTGATGCTTGGTTTCCTCAAAAGACTGCGGCA GACACTGTCATGAGATGGATTCCAAAGGCCGATTGGGGTTGTGCCGAAGATCCTTCAGGT AGATACGCCAAAATACACGAAAAGCACGTCAGTGCTTAA
Protein Properties
Pfam Domain Function
Protein Residues572
Protein Molecular Weight64592.5
Protein Theoretical pI5.69
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Asparagine synthetase [glutamine-hydrolyzing] 2 MCGIFAAFKHEDIHNFKPKALQLSKKIRHRGPDWSGNAVMNSTIFVHERLAIVGLDSGAQ PITSADGEYMLGVNGEIYNHIQLREMCSDYKFQTFSDCEPIIPLYLEHDIDAPKYLDGMF AFCLYDSKKDRIVAARDPIGVVTLYMGRSSQSPETVYFASELKCLTDVCDSIISFPPGHV YDSETDKITRYFTPDWLDEKRIPSTPVDYHAIRHSLEKAVRKRLMAEVPYGVLLSGGLDS SLIAAIAARETEKANADANEDNNVDEKQLAGIDDQGHLHTSGWSRLHSFAIGLPNAPDLQ AARKVAKFIGSIHHEHTFTLQEGLDALDDVIYHLETYDVTTIRASTPMFLLSRKIKAQGV KMVLSGEGSDEIFGGYLYFAQAPSAAEFHTESVQRVKNLHLADCLRANKSTMAWGLEARV PFLDKDFLQLCMNIDPNEKMIKPKEGRIEKYILRKAFDTTDEPDVKPYLPEEILWRQKEQ FSDGVGYSWIDGLRDTAERAISDAMFANPKADWGDDIPTTKEAYWYRLKFDAWFPQKTAA DTVMRWIPKADWGCAEDPSGRYAKIHEKHVSA
References
External Links
ResourceLink
Saccharomyces Genome Database ASN2
Uniprot IDP49090
Uniprot NameASNS2_YEAST
GenBank Gene IDX83099
Genebank Protein ID642347
General Reference
  • Van Dyck, L., Tettelin, H., Purnelle, B., Goffeau, A. (1997). "An 18.3 kb DNA fragment from yeast chromosome VII carries four unknown open reading frames, the gene for an Asn synthase, remnants of Ty and three tRNA genes." Yeast 13:171-176.9046098
  • Tettelin, H., Agostoni Carbone, M. L., Albermann, K., Albers, M., Arroyo, J., Backes, U., Barreiros, T., Bertani, I., Bjourson, A. J., Bruckner, M., Bruschi, C. V., Carignani, G., Castagnoli, L., Cerdan, E., Clemente, M. L., Coblenz, A., Coglievina, M., Coissac, E., Defoor, E., Del Bino, S., Delius, H., Delneri, D., de Wergifosse, P., Dujon, B., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Nature 387:81-84.9169869
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Chi, A., Huttenhower, C., Geer, L. Y., Coon, J. J., Syka, J. E., Bai, D. L., Shabanowitz, J., Burke, D. J., Troyanskaya, O. G., Hunt, D. F. (2007). "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Proc Natl Acad Sci U S A 104:2193-2198.17287358
  • Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956