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Identification
NameCarbamoyl-phosphate synthase arginine-specific large chain
Synonyms
  • Arginine-specific carbamoyl-phosphate synthetase, ammonia chain
Gene NameCPA2
Enzyme Class
Biological Properties
General FunctionInvolved in ATP binding
Specific Function2 ATP + L-glutamine + HCO(3)(-) + H(2)O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate
Cellular LocationCytoplasm
SMPDB Pathways
Pyrimidine metabolismPW002469 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Alanine, aspartate and glutamate metabolismec00250 Map00250
Pyrimidine metabolismec00240 Map00240
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00002L-GlutamineShow
YMDB00082CarbamoylphosphateShow
YMDB00109Adenosine triphosphateShow
YMDB00271L-Glutamic acidShow
YMDB00382Carbonic acidShow
YMDB00862hydronShow
YMDB00890waterShow
YMDB00907phosphateShow
YMDB00914ADPShow
GO Classification
Component
Not Available
Function
catalytic activity
ligase activity
ligase activity, forming carbon-nitrogen bonds
binding
nucleoside binding
purine nucleoside binding
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
carbamoyl-phosphate synthase activity
Process
metabolic process
nitrogen compound metabolic process
Gene Properties
Chromosome Locationchromosome 10
LocusYJR109C
Gene Sequence>3357 bp ATGACATCGATTTATACATCAACAGAGCCTACGAATTCTGCTTTTACTACCGAGGACTAC AAACCTCAATTAGTTGAAGGAGTAAATTCTGTACTTGTCATTGGATCAGGAGGGCTCTCT ATTGGTCAAGCTGGTGAATTCGATTACAGTGGTTCTCAAGCTATCAAAGCTCTGAAGGAA GATAACAAGTTTACTATATTGGTTAACCCAAATATCGCTACTAACCAGACTTCTCATTCC CTGGCGGACAAGATTTATTACTTGCCCGTTACACCAGAATACATCACATATATCATTGAA CTTGAAAGGCCGGATGCTATACTTTTAACCTTCGGTGGTCAAACAGGTCTAAATTGTGGG GTGGCTCTGGATGAATCTGGTGTTTTGGCTAAATACAACGTCAAAGTTTTAGGTACTCCT ATCAAAACTTTGATCACTTCTGAAGATAGGGATCTTTTCGCATCTGCGTTAAAGGATATC AACATTCCCATCGCAGAATCATTTGCTTGTGAAACCGTGGATGAAGCTTTGGAGGCTGCT GAAAGGGTCAAATACCCAGTTATTGTCAGATCTGCATACGCTTTGGGTGGGTTAGGCTCA GGTTTCGCTAACAATGCAAGTGAAATGAAGGAACTTGCCGCACAGTCCTTGTCGTTGGCC CCACAAATTCTTGTTGAAAAATCTTTGAAAGGTTGGAAAGAAGTTGAATATGAAGTGGTC AGAGATAGGGTTGGTAACTGTATTACAGTATGTAATATGGAAAATTTCGACCCACTTGGT GTTCATACTGGTGATTCTATGGTTTTTGCTCCTTCGCAGACCCTATCAGATGAAGAGTTT CATATGTTAAGATCCGCCGCAATTAAAATCATTAGACACCTTGGTGTTATTGGTGAATGT AATGTCCAATACGCTTTGCAACCTGATGGGCTAGACTATAGAGTTATTGAAGTGAACGCA CGTTTATCTCGTTCCTCTGCATTGGCGTCTAAGGCCACTGGTTATCCCTTAGCATACACT GCCGCCAAAATTGGGCTAGGCTATACTTTGCCAGAATTGCCAAACCCAATCACAAAAACT ACAGTGGCTAACTTTGAGCCATCTTTGGATTATATTGTGGCAAAAATACCTAAGTGGGAT CTTTCTAAGTTCCAATACGTGGACAGATCCATTGGTTCCTCTATGAAATCAGTTGGAGAA GTTATGGCTATTGGTAGAAACTATGAAGAAGCCTTTCAAAAAGCATTAAGACAGGTGGAT CCATCATTATTGGGATTCCAAGGTTCTACTGAATTCGGCGATCAACTTGATGAAGCCTTG AGAACTCCAACTGATAGAAGAGTCCTTGCCATTGGTCAGGCCTTAATCCATGAAAACTAT ACTGTTGAGAGAGTTAATGAATTGAGTAAAATTGATAAATGGTTTCTTTACAAGTGCATG AACATTGTTAATATCTATAAAGAGCTTGAATCAGTTAAATCTTTAAGTGACTTGAGTAAA GATCTCTTGCAGAGAGCCAAGAAATTAGGGTTTTCAGATAAGCAGATTGCGGTTACTATA AATAAACACGCCTCCACAAACATTAACGAACTGGAAATCAGAAGTTTAAGAAAAACGTTA GGTATAATCCCTTTTGTCAAGAGAATCGATACTTTGGCCGCAGAATTTCCAGCACAAACC AATTATTTGTATACCACTTACAATGCTACAAAGAACGATGTGGAGTTCAACGAAAATGGT ATGCTGGTTTTAGGCTCTGGTGTCTATCGTATTGGTTCATCTGTAGAATTTGATTGGTGT GCCGTGAACACCGCGAAGACATTAAGAGATCAAGGCAAGAAAACTATCATGATAAATTAT AACCCAGAAACAGTTTCCACAGATTTCGATGAAGTTGATAGATTATACTTTGAAGAATTA TCGTATGAAAGAGTGATGGACATTTATGAGTTGGAGCAATCTGAGGGTTGCATTATTTCT GTCGGTGGTCAATTACCTCAAAACATTGCCTTGAAACTTTACGATAACGGCTGTAATATA ATGGGTACCAATCCAAACGATATTGATAGAGCTGAGAACAGACACAAATTCTCATCTATT TTGGATTCTATTGATGTTGACCAACCTGAATGGAGTGAATTAACATCAGTAGAAGAAGCA AAATTATTTGCTTCTAAAGTTAACTACCCTGTGTTGATTCGTCCCTCATATGTTCTTTCC GGTGCGGCAATGAGTGTTGTTAATAATGAGGAGGAACTGAAGGCTAAATTAACTTTGGCA TCTGACGTTTCTCCAGACCATCCAGTCGTCATGTCTAAATTTATTGAAGGTGCTCAAGAA ATTGATGTGGACGCCGTTGCTTATAATGGTAATGTCTTGGTACATGCCATTTCCGAGCAT GTTGAAAATGCGGGTGTGCACTCCGGTGATGCTTCTTTAGTCTTACCGCCACAACATCTT TCTGACGATGTGAAGATTGCCCTAAAAGACATTGCTGATAAGGTCGCAAAAGCTTGGAAG ATCACTGGCCCCTTCAATATGCAAATCATCAAGGATGGGGAGCATACATTGAAAGTGATT GAATGTAACATTAGAGCTTCTAGATCATTTCCATTCGTTTCAAAAGTTTTAGGCGTTAAT TTTATTGAAATTGCTGTCAAGGCATTTTTGGGCGGTGACATTGTACCAAAACCTGTTGAT TTGATGCTCAACAAAAAGTACGACTATGTTGCTACTAAAGTTCCTCAATTTTCCTTTACA AGGTTGGCTGGTGCAGATCCTTTCTTAGGGGTTGAAATGGCATCAACTGGTGAAGTTGCT TCATTTGGTAGAGATTTAATTGAAAGCTATTGGACTGCTATTCAAAGTACCATGAACTTC CATGTACCACTACCTCCAAGTGGTATATTATTTGGAGGTGATACATCTCGAGAATACTTG GGCCAAGTGGCTTCCATAGTGGCCACTATTGGTTACAGAATATACACAACTAATGAGACC ACTAAAACGTATCTACAGGAACACATCAAAGAAAAGAACGCAAAGGTTTCTTTGATTAAA TTTCCAAAGAATGATAAGAGAAAATTGCGTGAACTATTTCAAGAATATGACATAAAAGCT GTTTTCAATTTAGCCTCCAAGAGAGCTGAGAGCACTGACGATGTTGACTATATTATGAGA AGGAATGCTATTGATTTTGCTATCCCATTGTTCAATGAACCTCAAACGGCTTTGTTATTT GCAAAGTGTTTGAAGGCAAAAATTGCAGAAAAGATCAAAATTTTGGAATCTCATGACGTT ATAGTTCCACCAGAAGTCCGTTCCTGGGATGAATTTATTGGTTTCAAAGCATATTGA
Protein Properties
Pfam Domain Function
Protein Residues1118
Protein Molecular Weight123914.0
Protein Theoretical pI4.95
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Carbamoyl-phosphate synthase arginine-specific large chain MTSIYTSTEPTNSAFTTEDYKPQLVEGVNSVLVIGSGGLSIGQAGEFDYSGSQAIKALKE DNKFTILVNPNIATNQTSHSLADKIYYLPVTPEYITYIIELERPDAILLTFGGQTGLNCG VALDESGVLAKYNVKVLGTPIKTLITSEDRDLFASALKDINIPIAESFACETVDEALEAA ERVKYPVIVRSAYALGGLGSGFANNASEMKELAAQSLSLAPQILVEKSLKGWKEVEYEVV RDRVGNCITVCNMENFDPLGVHTGDSMVFAPSQTLSDEEFHMLRSAAIKIIRHLGVIGEC NVQYALQPDGLDYRVIEVNARLSRSSALASKATGYPLAYTAAKIGLGYTLPELPNPITKT TVANFEPSLDYIVAKIPKWDLSKFQYVDRSIGSSMKSVGEVMAIGRNYEEAFQKALRQVD PSLLGFQGSTEFGDQLDEALRTPTDRRVLAIGQALIHENYTVERVNELSKIDKWFLYKCM NIVNIYKELESVKSLSDLSKDLLQRAKKLGFSDKQIAVTINKHASTNINELEIRSLRKTL GIIPFVKRIDTLAAEFPAQTNYLYTTYNATKNDVEFNENGMLVLGSGVYRIGSSVEFDWC AVNTAKTLRDQGKKTIMINYNPETVSTDFDEVDRLYFEELSYERVMDIYELEQSEGCIIS VGGQLPQNIALKLYDNGCNIMGTNPNDIDRAENRHKFSSILDSIDVDQPEWSELTSVEEA KLFASKVNYPVLIRPSYVLSGAAMSVVNNEEELKAKLTLASDVSPDHPVVMSKFIEGAQE IDVDAVAYNGNVLVHAISEHVENAGVHSGDASLVLPPQHLSDDVKIALKDIADKVAKAWK ITGPFNMQIIKDGEHTLKVIECNIRASRSFPFVSKVLGVNFIEIAVKAFLGGDIVPKPVD LMLNKKYDYVATKVPQFSFTRLAGADPFLGVEMASTGEVASFGRDLIESYWTAIQSTMNF HVPLPPSGILFGGDTSREYLGQVASIVATIGYRIYTTNETTKTYLQEHIKEKNAKVSLIK FPKNDKRKLRELFQEYDIKAVFNLASKRAESTDDVDYIMRRNAIDFAIPLFNEPQTALLF AKCLKAKIAEKIKILESHDVIVPPEVRSWDEFIGFKAY
References
External Links
ResourceLink
Saccharomyces Genome Database CPA2
Uniprot IDP03965
Uniprot NameCARB_YEAST
GenBank Gene IDK01178
Genebank Protein ID171310
General Reference
  • Lusty, C. J., Widgren, E. E., Broglie, K. E., Nyunoya, H. (1983). "Yeast carbamyl phosphate synthetase. Structure of the yeast gene and homology to Escherichia coli carbamyl phosphate synthetase." J Biol Chem 258:14466-14477.6358221
  • Galibert, F., Alexandraki, D., Baur, A., Boles, E., Chalwatzis, N., Chuat, J. C., Coster, F., Cziepluch, C., De Haan, M., Domdey, H., Durand, P., Entian, K. D., Gatius, M., Goffeau, A., Grivell, L. A., Hennemann, A., Herbert, C. J., Heumann, K., Hilger, F., Hollenberg, C. P., Huang, M. E., Jacq, C., Jauniaux, J. C., Katsoulou, C., Karpfinger-Hartl, L., et, a. l. .. (1996). "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." EMBO J 15:2031-2049.8641269
  • Kinney, D. M., Lusty, C. J. (1989). "Arginine restriction induced by delta-N-(phosphonacetyl)-L-ornithine signals increased expression of HIS3, TRP5, CPA1, and CPA2 in Saccharomyces cerevisiae." Mol Cell Biol 9:4882-4888.2689869
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106