Probable prolyl-tRNA synthetase, mitochondrial (P39965)

Identification
NameProbable prolyl-tRNA synthetase, mitochondrial
Synonyms
  • Altered inheritance rate of mitochondria protein 10
  • Proline--tRNA ligase
  • ProRS
Gene NameAIM10
Enzyme Class
Biological Properties
General FunctionInvolved in nucleotide binding
Specific FunctionATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)
Cellular LocationMitochondrion
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00097Adenosine monophosphateShow
YMDB00109Adenosine triphosphateShow
YMDB00219PyrophosphateShow
YMDB00378L-ProlineShow
GO Classification
Component
intracellular part
cytoplasm
cell part
Function
nucleoside binding
purine nucleoside binding
adenyl nucleotide binding
proline-tRNA ligase activity
adenyl ribonucleotide binding
ATP binding
ligase activity, forming carbon-oxygen bonds
ligase activity, forming aminoacyl-tRNA and related compounds
catalytic activity
aminoacyl-tRNA ligase activity
nucleotide binding
ligase activity
binding
Process
tRNA aminoacylation for protein translation
macromolecule biosynthetic process
cellular macromolecule biosynthetic process
translation
prolyl-tRNA aminoacylation
metabolic process
macromolecule metabolic process
cellular macromolecule metabolic process
RNA metabolic process
ncRNA metabolic process
biosynthetic process
tRNA metabolic process
tRNA aminoacylation
Gene Properties
Chromosome Locationchromosome 5
LocusYER087W
Gene Sequence>1731 bp ATGTCAGCTACTCTACTAAAGCAACCATTATGTACGGTTGTTCGGCAAGGTAAACAGTCC AAAGTGTCTGGATTGAACCTTTTGAGACTAAAGGCTCATTTGCACAGACAACACCTGTCA CCTTCCTTGATAAAACTACACTCTGAATTGAAATTGGATGAGCTGCAAACTGATAACACC CCTGATTACGTCCGTTTAGTTTTAAGGTCCTCTGTATACGATGTTATTAATGAATCTCCA ATCTCTCAAGGTGTAGGTTTGTCTTCCCGTCTAAACACGAATGTCATCTTGAAAAGAGAA GATCTATTGCCTGTTTTCTCTTTCAAGCTTCGTGGTGCCTATAACATGATTGCCAAGTTG GACGATTCTCAAAGAAACCAGGGTGTTATTGCCTGTTCAGCTGGGAATCATGCCCAAGGT GTGGCCTTTGCTGCTAAACACTTGAAAATACCTGCTACTATCGTTATGCCTGTTTGTACA CCATCTATTAAGTATCAAAATGTCTCGAGATTAGGGTCTCAAGTCGTCCTATATGGTAAC GATTTTGACGAGGCTAAGGCTGAATGTGCCAAATTGGCTGAAGAGCGTGGCTTGACGAAC ATTCCTCCTTTCGATCATCCTTATGTCATTGCCGGTCAAGGTACTGTAGCTATGGAAATC CTAAGACAAGTACGTACCGCTAATAAGATCGGTGCTGTCTTTGTTCCCGTCGGCGGTGGT GGTTTAATTGCTGGTATTGGTGCTTATTTGAAAAGGGTTGCTCCTCATATCAAAATCATT GGTGTTGAAACTTACGATGCGGCCACTTTACATAATTCCTTGCAACGCAACCAGAGAACT CCTTTACCTGTGGTGGGTACTTTTGCCGATGGTACGTCTGTGCGTATGATTGGTGAAGAA ACATTTAGAGTCGCCCAACAAGTGGTTGATGAAGTTGTTCTTGTTAACACTGACGAAATC TGTGCTGCAGTAAAGGATATTTTTGAAGATACTAGAAGTATTGTAGAACCATCTGGTGCC CTTTCAGTAGCCGGTATGAAGAAATACATCTCTACCGTACATCCAGAAATTGACCACACT AAAAACACCTATGTTCCCATCCTTTCTGGTGCTAACATGAACTTTGATAGATTAAGATTT GTTTCCGAACGTGCTGTTCTTGGTGAAGGAAAGGAAGTCTTCATGTTAGTTACTTTACCC GACGTCCCTGGTGCGTTCAAGAAAATGCAAAAGATCATCCACCCAAGATCTGTCACTGAA TTCTCTTACCGTTACAATGAACATCGTCATGAGTCCTCTAGTGAAGTGCCCAAGGCTTAC ATTTACACTTCTTTCAGCGTCGTTGACAGAGAAAAGGAAATCAAGCAAGTTATGCAACAG TTGAATGCTTTAGGTTTTGAAGCTGTGGATATCTCCGATAACGAATTGGCTAAATCTCAT GGTAGATACTTGGTTGGTGGTGCTTCTAAGGTTCCTAATGAAAGAATTATTTCATTTGAA TTCCCTGAAAGACCAGGTGCCTTGACTAGGTTCCTTGGAGGCCTAAGCGATTCTTGGAAT CTTACTTTATTCCATTATAGAAACCATGGTGCCGATATCGGTAAGGTTTTAGCTGGTATT TCCGTTCCTCCAAGGGAAAACTTAACCTTCCAAAAATTCTTGGAAGATTTAGGCTACACT TATCATGATGAAACTGATAACACTGTTTATCAAAAATTCTTGAAATATTAA
Protein Properties
Pfam Domain Function
Protein Residues576
Protein Molecular Weight65879.70313
Protein Theoretical pI7.45
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Probable prolyl-tRNA synthetase, mitochondrial MLKYRTLSRSCHIFHPKSLSNNTLKSETTQELLQTVGFVRRSQVGLFQWLPLGLRSLNKV SNAIRNRMDSDGGAIEVSLSAISSKALWQATDRWNNSELFKLKDSKGKQYCLTATCEEDI TDLMKNYIASYKDMPITIYQMTRKYRDEIRPRGGILRGREFLMKDAYSFASNEEDAFASF QKLDDTYNKIFKDLKIPFVSAWADSGDIGGEFSKEFHLIHESGEDTLMSCKHCGDISTLD MSQSYPEKDGQYSGDVDCKYALTKDHSTLICFYYPKDRQLNWNLALNAMDKDIDLTLRNK PNDHVLQVYEKDNEDIMFSKILRVMDCRLNSKSNFPDFPLKKYLKNNFGQISDVSIVDAQ ENEICGKCEEGRLEPLKSIEVGHIFLLGNKYSKPLNVKFVDKENKNETFVHMGCYGIGVS RLVGAIAELGRDSNGFRWPAIMAPYKVSICTGPNNPENSQRLQDVKSELLNDPTMQNLQN DILDQFNEKLGIGARIKLSHAMGIPLCVIVGSKSWPNVEIEVRGIRWGEKDLWRKQFEKR CSELQWKCTKNEHGIEKHTVPIQHLAEVIGVLLKDM
References
External Links
ResourceLink
Saccharomyces Genome Database AIM10
Uniprot IDP39965
Uniprot NameSYPM_YEAST
GenBank Gene IDU18839
Genebank Protein ID603324
General Reference
  • Dietrich, F. S., Mulligan, J., Hennessy, K., Yelton, M. A., Allen, E., Araujo, R., Aviles, E., Berno, A., Brennan, T., Carpenter, J., Chen, E., Cherry, J. M., Chung, E., Duncan, M., Guzman, E., Hartzell, G., Hunicke-Smith, S., Hyman, R. W., Kayser, A., Komp, C., Lashkari, D., Lew, H., Lin, D., Mosedale, D., Davis, R. W., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Nature 387:78-81.9169868
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Reinders, J., Zahedi, R. P., Pfanner, N., Meisinger, C., Sickmann, A. (2006). "Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics." J Proteome Res 5:1543-1554.16823961
  • Chi, A., Huttenhower, C., Geer, L. Y., Coon, J. J., Syka, J. E., Bai, D. L., Shabanowitz, J., Burke, D. J., Troyanskaya, O. G., Hunt, D. F. (2007). "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Proc Natl Acad Sci U S A 104:2193-2198.17287358