Leucyl-tRNA synthetase, cytoplasmic (P26637)

Identification
NameLeucyl-tRNA synthetase, cytoplasmic
Synonyms
  • Leucine--tRNA ligase
  • LeuRS
Gene NameCDC60
Enzyme Class
Biological Properties
General FunctionInvolved in nucleotide binding
Specific FunctionATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)
Cellular LocationCytoplasm
SMPDB PathwaysNot Available
KEGG Pathways
Valine, leucine and isoleucine biosynthesisec00290 Map00290
SMPDB ReactionsNot Available
KEGG Reactions
L-Leucine + Adenosine triphosphate + tRNA(Leu) → Adenosine monophosphate + Pyrophosphate + Leu-tRNA(Leu)
Metabolites
YMDB IDNameView
YMDB00097Adenosine monophosphateShow
YMDB00109Adenosine triphosphateShow
YMDB00219PyrophosphateShow
YMDB00387L-LeucineShow
GO Classification
Component
intracellular part
cytoplasm
cell part
Function
binding
nucleoside binding
purine nucleoside binding
leucine-tRNA ligase activity
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
ligase activity, forming carbon-oxygen bonds
ligase activity, forming aminoacyl-tRNA and related compounds
catalytic activity
aminoacyl-tRNA ligase activity
nucleotide binding
ligase activity
Process
tRNA aminoacylation
tRNA aminoacylation for protein translation
macromolecule biosynthetic process
cellular macromolecule biosynthetic process
translation
leucyl-tRNA aminoacylation
metabolic process
macromolecule metabolic process
cellular macromolecule metabolic process
RNA metabolic process
ncRNA metabolic process
biosynthetic process
tRNA metabolic process
Gene Properties
Chromosome Locationchromosome 16
LocusYPL160W
Gene Sequence>3273 bp ATGTCTTCTGGTTTGGTCTTAGAAAACACGGCACGTAGAGATGCGTTAATCGCCATCGAA AAGAAGTACCAAAAAATATGGGCTGAAGAGCACCAATTTGAAATCGATGCGCCATCAATC GAAGATGAGCCCATTACAATGGACTCTGAAGAGTTACATCGCACGTATCCTAAATTCATG TCGTCTATGGCCTATCCTTATATGAACGGTGTTATGCACGCCGGCCATTGTTTTACTCTC TCCAAAGTCGAATTTTCTATTGGTTTTGAAAGGATGAACGGTAAAAGAGCTTTGTTTCCA TTGGGTTTTCACTGTACAGGTATGCCAATCTTAGCATGTGCCGATAAACTAAAGAGGGAA GCCGAACTTTTTGGCAAGAACTTCGACAATGTACCAGCAGAAGAAGAAGAAATTAAAGAA GAAACGCCGGCTGAGAAAGACCACGAGGATGTTACCAAATTCAAAGCTAAAAAATCTAAA GCCGCAGCCAAGAAGGGCCGTGGTAAGTACCAATTCGAAATCATGTTACAGCTTGGTATC CCTAGAGAAGAAATCATTAAGTTCGCCGACGCCAAGTACTGGTTGACCTATTTCCCACCT TTGTGTGAAAGTGATTGTACTTCACTTGGGGCTCGTATCGATTGGAGACGTTCTTTTGTC ACCACTGATGCCAATCCTTATTACGATGCCTTTATTAGATGGCAAATGAACAAGTTAAAG GCTGCTGGTAAAATCAAATTCGGTGAACGTTATACTATTTACTCTGAAAAAGATGGTCAA GCTTGTATGGATCACGATAGACAATCCGGTGAAGGTGTTACACCACAAGAATATATTGGT GTGAAAATTGAAGCTTTAGAGTTCGCCGATGATGCCGCAAAAATCATTGATTCAAGCTCA GATCTTGACAAATCCAAAAAATTCTACTTTGTCGCTGCCACGTTGAGACCGGAAACTATG TATGGACAAACATGTTGTTTTGTTTCTCCAACAATTGAGTATGGTATATTTGACGCTGGT GATTCTTATTTTATCACTACTGAACGTGCATTCAAGAACATGTCTTACCAAAAGCTAACA CCAAAGAGAGGCTTCTACAAGCCGATAGTTACTGTCCCAGGTAAGGCATTTATCGGTACC AAGATTCATGCCCCACAATCTGTATATCCAGAACTAAGAATATTGCCTATGGAAACTGTC ATTGCTACCAAGGGTACTGGTGTCGTCACGTGTGTACCATCTAATTCACCAGATGACTAC ATTACCACCAAGGACTTGTTGCACAAACCTGAATACTACGGCATTAAACCTGAATGGATT GACCATGAAATTGTTCCAATTATGCATACCGAAAAGTATGGTGATTTAACTGCAAAGGCT ATTGTTGAAGAAAAAAAAATTCAATCTCCAAAGGATAAGAACTTGTTAGCAGAAGCCAAG AAAATTGCTTACAAGGAAGATTACTACACTGGTACCATGATATATGGTCCTTATAAAGGT GAGAAGGTCGAACAAGCCAAAAACAAGGTTAAGGCTGATATGATTGCCGCCGGTGAAGCT TTCGTCTACAACGAACCAGAATCTCAGGTCATGTCACGTTCTGGTGATGACTGTATCGTT TCTTTGGAAGATCAATGGTACGTTGATTACGGTGAAGAAAGTTGGAAGAAACAAGCAATT GAATGTTTGGAAGGGATGCAATTATTTGCTCCTGAGGTAAAAAACGCATTTGAAGGTGTT TTGGATTGGTTGAAAAACTGGGCTGTTTGCCGTACATACGGGTTAGGTACCAGATTGCCT TGGGATGAAAAATACTTAGTTGAGTCTTTATCAGACTCTACTATTTATCAATCATTTTAT ACCATTGCTCACTTACTATTTAAGGATTATTATGGTAACGAAATTGGACCATTAGGCATT TCAGCTGATCAAATGACAGATGAGGTTTTCGATTACATTTTCCAACATCAGGATGATGTC AAGAACACCAATATTCCTTTGCCTGCTTTACAAAAGTTGAGAAGGGAATTTGAGTATTTC TACCCATTAGACGTCTCAATTTCCGGGAAGGATTTGATCCCTAACCATTTAACTTTCTTC ATTTACACACATGTTGCATTATTCCCAAAGAAATTCTGGCCTAAAGGTATCAGAGCTAAC GGTCATTTGATGTTAAACAATTCTAAAATGTCGAAATCTACAGGTAATTTTATGACTTTA GAACAAACGGTTGAAAAGTTCGGTGCTGATGCAGCTCGTATTGCGTTTGCCGATGCGGGA GATACTGTTGAAGATGCAAATTTTGACGAATCTAACGCTAATGCTGCTATTCTGAGATTA TTCAATTTGAAGGAGTGGGCAGAAGAAATTACCAAGGAATCTAACTTGAGAACTGGGGAA ATTACAGATTTCTTTGATATCGCGTTTGAACACGAAATGAATGCTCTGATTGAAAAGACT TATGAACAGTATGCATTGACTAATTATAAGAATGCCTTGAAATACGGTCTTTTTGATTTC CAAGCTGCTAGGGATTACTATCGTGAAGCTAGTGGTGTAATGCATAAAGATTTAATTGCA CGTTATATTGAAACACAAGCCTTATTGCTGGCTCCAATTGCGCCTCATTTCGCTGAGTAT ATTTACCGTGAAGTTCTAGGTAACCAAACCTCTGTACAAAATGCCAAATTTCCACGTGCA TCCAAACCAGTAGATAAGGGTGTTCTGGCTGCTTTGGATTACTTGAGAAATCTACAGAGA AGCATAAGAGAAGGCGAAGGGCAAGCACTAAAAAAGAAGAAGGGCAAGTCTGCTGAAATC GATGCATCCAAACCTGTTAAGTTGACTTTATTGATCTCTGAATCATTTCCAGAATGGCAA TCACAATGCGTGGAAATCGTCCGTAAACTATTCAGCGAACAGACTTTAGACGATAACAAA AAGGTCAGAGAGCATATTGAGCCTAAAGAAATGAAGAGAGCTATGCCATTCATTTCTCTA TTGAAACAACGTTTAGCTAATGAAAAGCCAGAGGATGTGTTTGAAAGAGAGTTACAATTC AGCGAAATCGATACTGTCAAGGCTGCTGCAAGGAATGTTAAGAAGGCAGCACAAGCTTTG AAAATTGCTGAATTTAGCGCAATTTCTTTCCCATATGGCGCCAAAACCGGTAAGGATATC TTTACTGGTGAAGAAGTTGAAATTCCACCTGTAACAAAGATTGTTGAAAATGCTGTTCCA GGTAACCCAGGTGTTGTCTTCCAAAATATTTAA
Protein Properties
Pfam Domain Function
Protein Residues1090
Protein Molecular Weight124140.0
Protein Theoretical pI5.6
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Leucyl-tRNA synthetase, cytoplasmic MSSGLVLENTARRDALIAIEKKYQKIWAEEHQFEIDAPSIEDEPITMDSEELHRTYPKFM SSMAYPYMNGVMHAGHCFTLSKVEFSIGFERMNGKRALFPLGFHCTGMPILACADKLKRE AELFGKNFDNVPAEEEEIKEETPAEKDHEDVTKFKAKKSKAAAKKGRGKYQFEIMLQLGI PREEIIKFADAKYWLTYFPPLCESDCTSLGARIDWRRSFVTTDANPYYDAFIRWQMNKLK AAGKIKFGERYTIYSEKDGQACMDHDRQSGEGVTPQEYIGVKIEALEFADDAAKIIDSSS DLDKSKKFYFVAATLRPETMYGQTCCFVSPTIEYGIFDAGDSYFITTERAFKNMSYQKLT PKRGFYKPIVTVPGKAFIGTKIHAPQSVYPELRILPMETVIATKGTGVVTCVPSNSPDDY ITTKDLLHKPEYYGIKPEWIDHEIVPIMHTEKYGDLTAKAIVEEKKIQSPKDKNLLAEAK KIAYKEDYYTGTMIYGPYKGEKVEQAKNKVKADMIAAGEAFVYNEPESQVMSRSGDDCIV SLEDQWYVDYGEESWKKQAIECLEGMQLFAPEVKNAFEGVLDWLKNWAVCRTYGLGTRLP WDEKYLVESLSDSTIYQSFYTIAHLLFKDYYGNEIGPLGISADQMTDEVFDYIFQHQDDV KNTNIPLPALQKLRREFEYFYPLDVSISGKDLIPNHLTFFIYTHVALFPKKFWPKGIRAN GHLMLNNSKMSKSTGNFMTLEQTVEKFGADAARIAFADAGDTVEDANFDESNANAAILRL FNLKEWAEEITKESNLRTGEITDFFDIAFEHEMNALIEKTYEQYALTNYKNALKYGLFDF QAARDYYREASGVMHKDLIARYIETQALLLAPIAPHFAEYIYREVLGNQTSVQNAKFPRA SKPVDKGVLAALDYLRNLQRSIREGEGQALKKKKGKSAEIDASKPVKLTLLISESFPEWQ SQCVEIVRKLFSEQTLDDNKKVREHIEPKEMKRAMPFISLLKQRLANEKPEDVFERELQF SEIDTVKAAARNVKKAAQALKIAEFSAISFPYGAKTGKDIFTGEEVEIPPVTKIVENAVP GNPGVVFQNI
References
External Links
ResourceLink
Saccharomyces Genome Database CDC60
Uniprot IDP26637
Uniprot NameSYLC_YEAST
GenBank Gene IDX62878
Genebank Protein ID3508
General Reference
  • Hohmann, S., Thevelein, J. M. (1992). "The cell division cycle gene CDC60 encodes cytosolic leucyl-tRNA synthetase in Saccharomyces cerevisiae." Gene 120:43-49.1398122
  • Purnelle, B., Coster, F., Goffeau, A. (1996). "The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators." Yeast 12:1483-1492.8948103
  • Bussey, H., Storms, R. K., Ahmed, A., Albermann, K., Allen, E., Ansorge, W., Araujo, R., Aparicio, A., Barrell, B., Badcock, K., Benes, V., Botstein, D., Bowman, S., Bruckner, M., Carpenter, J., Cherry, J. M., Chung, E., Churcher, C., Coster, F., Davis, K., Davis, R. W., Dietrich, F. S., Delius, H., DiPaolo, T., Hani, J., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Nature 387:103-105.9169875
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956