You are using an unsupported browser. Please upgrade your browser to a newer version to get the best experience on Yeast Metabolome Database.
| Identification |
|---|
| Name | Carbamoyl-phosphate synthase arginine-specific large chain |
|---|
| Synonyms | - Arginine-specific carbamoyl-phosphate synthetase, ammonia chain
|
|---|
| Gene Name | CPA2 |
|---|
| Enzyme Class | |
|---|
| Biological Properties |
|---|
| General Function | Involved in ATP binding |
|---|
| Specific Function | 2 ATP + L-glutamine + HCO(3)(-) + H(2)O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate |
|---|
| Cellular Location | Cytoplasm |
|---|
| SMPDB Pathways | |
|---|
| KEGG Pathways | | Alanine, aspartate and glutamate metabolism | ec00250 |  | | Pyrimidine metabolism | ec00240 |  |
|
|---|
| SMPDB Reactions | Not Available |
|---|
| KEGG Reactions | Not Available |
|---|
| Metabolites | | YMDB ID | Name | View |
|---|
| YMDB00002 | L-Glutamine | Show | | YMDB00082 | Carbamoylphosphate | Show | | YMDB00109 | Adenosine triphosphate | Show | | YMDB00271 | L-Glutamic acid | Show | | YMDB00382 | Carbonic acid | Show | | YMDB00862 | hydron | Show | | YMDB00890 | water | Show | | YMDB00907 | phosphate | Show | | YMDB00914 | ADP | Show |
|
|---|
| GO Classification | | Component |
|---|
| Not Available | | Function |
|---|
| purine nucleoside binding | | adenyl nucleotide binding | | adenyl ribonucleotide binding | | ATP binding | | carbamoyl-phosphate synthase activity | | catalytic activity | | ligase activity | | ligase activity, forming carbon-nitrogen bonds | | binding | | nucleoside binding | | Process |
|---|
| metabolic process | | nitrogen compound metabolic process |
|
|---|
| Gene Properties |
|---|
| Chromosome Location | chromosome 10 |
|---|
| Locus | YJR109C |
|---|
| Gene Sequence | >3357 bp
ATGACATCGATTTATACATCAACAGAGCCTACGAATTCTGCTTTTACTACCGAGGACTAC
AAACCTCAATTAGTTGAAGGAGTAAATTCTGTACTTGTCATTGGATCAGGAGGGCTCTCT
ATTGGTCAAGCTGGTGAATTCGATTACAGTGGTTCTCAAGCTATCAAAGCTCTGAAGGAA
GATAACAAGTTTACTATATTGGTTAACCCAAATATCGCTACTAACCAGACTTCTCATTCC
CTGGCGGACAAGATTTATTACTTGCCCGTTACACCAGAATACATCACATATATCATTGAA
CTTGAAAGGCCGGATGCTATACTTTTAACCTTCGGTGGTCAAACAGGTCTAAATTGTGGG
GTGGCTCTGGATGAATCTGGTGTTTTGGCTAAATACAACGTCAAAGTTTTAGGTACTCCT
ATCAAAACTTTGATCACTTCTGAAGATAGGGATCTTTTCGCATCTGCGTTAAAGGATATC
AACATTCCCATCGCAGAATCATTTGCTTGTGAAACCGTGGATGAAGCTTTGGAGGCTGCT
GAAAGGGTCAAATACCCAGTTATTGTCAGATCTGCATACGCTTTGGGTGGGTTAGGCTCA
GGTTTCGCTAACAATGCAAGTGAAATGAAGGAACTTGCCGCACAGTCCTTGTCGTTGGCC
CCACAAATTCTTGTTGAAAAATCTTTGAAAGGTTGGAAAGAAGTTGAATATGAAGTGGTC
AGAGATAGGGTTGGTAACTGTATTACAGTATGTAATATGGAAAATTTCGACCCACTTGGT
GTTCATACTGGTGATTCTATGGTTTTTGCTCCTTCGCAGACCCTATCAGATGAAGAGTTT
CATATGTTAAGATCCGCCGCAATTAAAATCATTAGACACCTTGGTGTTATTGGTGAATGT
AATGTCCAATACGCTTTGCAACCTGATGGGCTAGACTATAGAGTTATTGAAGTGAACGCA
CGTTTATCTCGTTCCTCTGCATTGGCGTCTAAGGCCACTGGTTATCCCTTAGCATACACT
GCCGCCAAAATTGGGCTAGGCTATACTTTGCCAGAATTGCCAAACCCAATCACAAAAACT
ACAGTGGCTAACTTTGAGCCATCTTTGGATTATATTGTGGCAAAAATACCTAAGTGGGAT
CTTTCTAAGTTCCAATACGTGGACAGATCCATTGGTTCCTCTATGAAATCAGTTGGAGAA
GTTATGGCTATTGGTAGAAACTATGAAGAAGCCTTTCAAAAAGCATTAAGACAGGTGGAT
CCATCATTATTGGGATTCCAAGGTTCTACTGAATTCGGCGATCAACTTGATGAAGCCTTG
AGAACTCCAACTGATAGAAGAGTCCTTGCCATTGGTCAGGCCTTAATCCATGAAAACTAT
ACTGTTGAGAGAGTTAATGAATTGAGTAAAATTGATAAATGGTTTCTTTACAAGTGCATG
AACATTGTTAATATCTATAAAGAGCTTGAATCAGTTAAATCTTTAAGTGACTTGAGTAAA
GATCTCTTGCAGAGAGCCAAGAAATTAGGGTTTTCAGATAAGCAGATTGCGGTTACTATA
AATAAACACGCCTCCACAAACATTAACGAACTGGAAATCAGAAGTTTAAGAAAAACGTTA
GGTATAATCCCTTTTGTCAAGAGAATCGATACTTTGGCCGCAGAATTTCCAGCACAAACC
AATTATTTGTATACCACTTACAATGCTACAAAGAACGATGTGGAGTTCAACGAAAATGGT
ATGCTGGTTTTAGGCTCTGGTGTCTATCGTATTGGTTCATCTGTAGAATTTGATTGGTGT
GCCGTGAACACCGCGAAGACATTAAGAGATCAAGGCAAGAAAACTATCATGATAAATTAT
AACCCAGAAACAGTTTCCACAGATTTCGATGAAGTTGATAGATTATACTTTGAAGAATTA
TCGTATGAAAGAGTGATGGACATTTATGAGTTGGAGCAATCTGAGGGTTGCATTATTTCT
GTCGGTGGTCAATTACCTCAAAACATTGCCTTGAAACTTTACGATAACGGCTGTAATATA
ATGGGTACCAATCCAAACGATATTGATAGAGCTGAGAACAGACACAAATTCTCATCTATT
TTGGATTCTATTGATGTTGACCAACCTGAATGGAGTGAATTAACATCAGTAGAAGAAGCA
AAATTATTTGCTTCTAAAGTTAACTACCCTGTGTTGATTCGTCCCTCATATGTTCTTTCC
GGTGCGGCAATGAGTGTTGTTAATAATGAGGAGGAACTGAAGGCTAAATTAACTTTGGCA
TCTGACGTTTCTCCAGACCATCCAGTCGTCATGTCTAAATTTATTGAAGGTGCTCAAGAA
ATTGATGTGGACGCCGTTGCTTATAATGGTAATGTCTTGGTACATGCCATTTCCGAGCAT
GTTGAAAATGCGGGTGTGCACTCCGGTGATGCTTCTTTAGTCTTACCGCCACAACATCTT
TCTGACGATGTGAAGATTGCCCTAAAAGACATTGCTGATAAGGTCGCAAAAGCTTGGAAG
ATCACTGGCCCCTTCAATATGCAAATCATCAAGGATGGGGAGCATACATTGAAAGTGATT
GAATGTAACATTAGAGCTTCTAGATCATTTCCATTCGTTTCAAAAGTTTTAGGCGTTAAT
TTTATTGAAATTGCTGTCAAGGCATTTTTGGGCGGTGACATTGTACCAAAACCTGTTGAT
TTGATGCTCAACAAAAAGTACGACTATGTTGCTACTAAAGTTCCTCAATTTTCCTTTACA
AGGTTGGCTGGTGCAGATCCTTTCTTAGGGGTTGAAATGGCATCAACTGGTGAAGTTGCT
TCATTTGGTAGAGATTTAATTGAAAGCTATTGGACTGCTATTCAAAGTACCATGAACTTC
CATGTACCACTACCTCCAAGTGGTATATTATTTGGAGGTGATACATCTCGAGAATACTTG
GGCCAAGTGGCTTCCATAGTGGCCACTATTGGTTACAGAATATACACAACTAATGAGACC
ACTAAAACGTATCTACAGGAACACATCAAAGAAAAGAACGCAAAGGTTTCTTTGATTAAA
TTTCCAAAGAATGATAAGAGAAAATTGCGTGAACTATTTCAAGAATATGACATAAAAGCT
GTTTTCAATTTAGCCTCCAAGAGAGCTGAGAGCACTGACGATGTTGACTATATTATGAGA
AGGAATGCTATTGATTTTGCTATCCCATTGTTCAATGAACCTCAAACGGCTTTGTTATTT
GCAAAGTGTTTGAAGGCAAAAATTGCAGAAAAGATCAAAATTTTGGAATCTCATGACGTT
ATAGTTCCACCAGAAGTCCGTTCCTGGGATGAATTTATTGGTTTCAAAGCATATTGA |
|---|
| Protein Properties |
|---|
| Pfam Domain Function | |
|---|
| Protein Residues | 1118 |
|---|
| Protein Molecular Weight | 123914.0 |
|---|
| Protein Theoretical pI | 4.95 |
|---|
| Signalling Regions | |
|---|
| Transmembrane Regions | |
|---|
| Protein Sequence | >Carbamoyl-phosphate synthase arginine-specific large chain
MTSIYTSTEPTNSAFTTEDYKPQLVEGVNSVLVIGSGGLSIGQAGEFDYSGSQAIKALKE
DNKFTILVNPNIATNQTSHSLADKIYYLPVTPEYITYIIELERPDAILLTFGGQTGLNCG
VALDESGVLAKYNVKVLGTPIKTLITSEDRDLFASALKDINIPIAESFACETVDEALEAA
ERVKYPVIVRSAYALGGLGSGFANNASEMKELAAQSLSLAPQILVEKSLKGWKEVEYEVV
RDRVGNCITVCNMENFDPLGVHTGDSMVFAPSQTLSDEEFHMLRSAAIKIIRHLGVIGEC
NVQYALQPDGLDYRVIEVNARLSRSSALASKATGYPLAYTAAKIGLGYTLPELPNPITKT
TVANFEPSLDYIVAKIPKWDLSKFQYVDRSIGSSMKSVGEVMAIGRNYEEAFQKALRQVD
PSLLGFQGSTEFGDQLDEALRTPTDRRVLAIGQALIHENYTVERVNELSKIDKWFLYKCM
NIVNIYKELESVKSLSDLSKDLLQRAKKLGFSDKQIAVTINKHASTNINELEIRSLRKTL
GIIPFVKRIDTLAAEFPAQTNYLYTTYNATKNDVEFNENGMLVLGSGVYRIGSSVEFDWC
AVNTAKTLRDQGKKTIMINYNPETVSTDFDEVDRLYFEELSYERVMDIYELEQSEGCIIS
VGGQLPQNIALKLYDNGCNIMGTNPNDIDRAENRHKFSSILDSIDVDQPEWSELTSVEEA
KLFASKVNYPVLIRPSYVLSGAAMSVVNNEEELKAKLTLASDVSPDHPVVMSKFIEGAQE
IDVDAVAYNGNVLVHAISEHVENAGVHSGDASLVLPPQHLSDDVKIALKDIADKVAKAWK
ITGPFNMQIIKDGEHTLKVIECNIRASRSFPFVSKVLGVNFIEIAVKAFLGGDIVPKPVD
LMLNKKYDYVATKVPQFSFTRLAGADPFLGVEMASTGEVASFGRDLIESYWTAIQSTMNF
HVPLPPSGILFGGDTSREYLGQVASIVATIGYRIYTTNETTKTYLQEHIKEKNAKVSLIK
FPKNDKRKLRELFQEYDIKAVFNLASKRAESTDDVDYIMRRNAIDFAIPLFNEPQTALLF
AKCLKAKIAEKIKILESHDVIVPPEVRSWDEFIGFKAY |
|---|
| References |
|---|
| External Links | |
|---|
| General Reference | - Lusty, C. J., Widgren, E. E., Broglie, K. E., Nyunoya, H. (1983). "Yeast carbamyl phosphate synthetase. Structure of the yeast gene and homology to Escherichia coli carbamyl phosphate synthetase." J Biol Chem 258:14466-14477.6358221
- Galibert, F., Alexandraki, D., Baur, A., Boles, E., Chalwatzis, N., Chuat, J. C., Coster, F., Cziepluch, C., De Haan, M., Domdey, H., Durand, P., Entian, K. D., Gatius, M., Goffeau, A., Grivell, L. A., Hennemann, A., Herbert, C. J., Heumann, K., Hilger, F., Hollenberg, C. P., Huang, M. E., Jacq, C., Jauniaux, J. C., Katsoulou, C., Karpfinger-Hartl, L., et, a. l. .. (1996). "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." EMBO J 15:2031-2049.8641269
- Kinney, D. M., Lusty, C. J. (1989). "Arginine restriction induced by delta-N-(phosphonacetyl)-L-ornithine signals increased expression of HIS3, TRP5, CPA1, and CPA2 in Saccharomyces cerevisiae." Mol Cell Biol 9:4882-4888.2689869
- Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
|
|---|