Probable phospholipid-transporting ATPase DNF3 (Q12674)

Identification

Name

Probable phospholipid-transporting ATPase DNF3

Synonyms

Not Available

Gene Name

DNF3

Enzyme Class

3.6.3.1

Biological Properties

General Function

Involved in nucleotide binding

Specific Function

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of phospholipids (Potential)

Cellular Location

Membrane; Multi-pass membrane protein

SMPDB Pathways

Lysolipid incorporation into ER	PW002532
Lysolipid incorporation into ER PC(10:0/10:0)	PW002780
Lysolipid incorporation into ER PC(14:0/14:0)	PW002783
Lysolipid incorporation into ER PC(16:0/16:0)	PW002784
Lysolipid incorporation into ER PC(16:1(11Z)/16:1(11Z))	PW002786

KEGG Pathways

Not Available

SMPDB Reactions

Not Available

KEGG Reactions

Not Available

Metabolites

YMDB ID	Name	View
YMDB00109	Adenosine triphosphate	Show
YMDB00890	water	Show
YMDB00907	phosphate	Show
YMDB00914	ADP	Show

GO Classification

Component
cell part
membrane
membrane part
intrinsic to membrane
integral to membrane
Function
phospholipid-translocating ATPase activity
hydrolase activity
substrate-specific transmembrane transporter activity
ion transmembrane transporter activity
binding
magnesium ion binding
catalytic activity
nucleoside binding
ATPase activity, coupled to transmembrane movement of ions
purine nucleoside binding
ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism
adenyl nucleotide binding
hydrolase activity, acting on acid anhydrides
adenyl ribonucleotide binding
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
ATP binding
ion binding
cation binding
metal ion binding
substrate-specific transporter activity
transporter activity
lipid transporter activity
phospholipid transporter activity
transmembrane transporter activity
Process
nucleobase, nucleoside and nucleotide metabolic process
establishment of localization
lipid transport
nucleoside phosphate metabolic process
transport
phospholipid transport
nucleotide metabolic process
purine nucleotide metabolic process
purine nucleotide biosynthetic process
metabolic process
purine nucleoside triphosphate biosynthetic process
purine ribonucleoside triphosphate biosynthetic process
ATP biosynthetic process
nitrogen compound metabolic process
cellular nitrogen compound metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process

Gene Properties

Chromosome Location

chromosome 13

Locus

YMR162C

Gene Sequence

>4971 bp ATGGGCATAGCTGATGGGCAAAGAAGAAGATCTTCATCACTACGAACGCAGATGTTCAAC AAGCATTTGTATGATAAATATCGCGGAAGAACTGATGATGAGATAGAGTTAGAAGATATA AATGAGAGTAAGACGTTCTCCGGTAGCGATAATAACGATAAAGATGACAGAGATGAAACA AGCGGCAATTATGCTGCTGAAGAGGATTATGAAATGGAAGAGTATGGTAGTCCTGATGTG TCATATAGCATTATCACTAAAATACTTGATACTATCCTAGATCGAAGGAGGACGTTCCAT TCGAAAGACGGTAGACATATTCCGATTATATTAGATCATAATGCAATTGAATACAAACAA GCTGCTACTAAAAGGGATGGTCATTTGATAGACGAAAGGTTCAACAAGCCTTACTGCGAC AACCGAATAACTTCATCAAGATATACATTCTATTCCTTTTTGCCTAGACAGTTGTATGCA CAATTTTCAAAGTTAGCCAATACTTATTTTTTCATTGTCGCAGTATTACAAATGATACCT GGATGGTCTACAACTGGTACTTATACAACTATTATCCCCTTATGTGTATTTATGGGTATA TCCATGACAAGAGAGGCTTGGGACGATTTCAGGAGACATCGTTTAGATAAGGAAGAGAAC AATAAACCAGTGGGAGTGTTGGTTAAAGATGGAAATAATGACGCACAAGAAGTTTATACA CTACCAAGTAGTGTTGTCTCATCCACTGCATATCTAACTAAATCGGCCGCTGCTGAAAAT AATCCACCTTTAAACGATGATCGGAACTCCTCTCAAGGACACTTCTTAGACACGCACTTC AATAACTTTGAACTACTCAAGAATAAATACAATGTTCATATTCATCAAAAGAAGTGGGAA AAACTTCGTGTTGGAGACTTCGTACTTCTCACACAAGATGATTGGGTACCTGCAGATCTG CTTTTATTGACATGTGACGGCGAAAATAGCGAATGTTTCGTTGAAACGATGGCTTTAGAT GGCGAAACAAATTTGAAAAGTAAACAACCCCATCCAGAATTAAATAAGTTAACCAAAGCC GCATCAGGTCTTGCAAATATTAATGCTCAAGTTACCGTGGAAGATCCCAATATTGATTTA TATAATTTTGAAGGTAATCTTGAATTGAAAAACCATCGCAATGATACCATAATGAAATAC CCTCTAGGTCCTGATAATGTCATTTATCGTGGTAGTATTTTGAGGAATACTCAAAATGTT GTTGGTATGGTCATTTTCAGTGGTGAGGAAACAAAAATCAGAATGAATGCTTTAAAAAAC CCAAGAACGAAAGCCCCTAAGCTACAAAGGAAGATAAATATGATTATCGTGTTTATGGTG TTTGTCGTTGCCACAATATCTTTATTTTCTTATCTTGGCCATGTTTTGCATAAGAAAAAA TATATCGACCAGAACAAAGCATGGTATCTATTTCAGGCAGATGCTGGCGTAGCACCCACA ATAATGTCTTTTATCATCATGTATAACACTGTGATTCCTTTGTCCCTATATGTAACAATG GAAATTATCAAGGTAGTCCAAAGTAAAATGATGGAATGGGACATAGACATGTACCATGCA GAAACGAACACGCCATGCGAATCGAGAACTGCCACCATTCTGGAGGAATTGGGGCAAGTA TCTTATATTTTTAGTGATAAAACCGGAACGCTGACGGATAATAAGATGATATTTCGGAAA TTTTCTTTATGTGGATCATCTTGGCTGCATAATGTAGATCTCGGTAATTCTGAGGATAAC TTTGAAGACAACAGAGACAATACGAACTCCTTGAGACTACCACCAAAAGCTCACAACGGC TCTAGTATAGACGTTGTTTCCATTGGAGATCAAAATGTTCTTGATCGGTTAGGATTTTCG GACGCACCAATTGAAAAAGGGCATCGTCCATCTTTGGACAACTTTCCTAAATCTAGGAAT TCTATTGAATATAAGGGAAATTCATCAGCAATTTATACAGGCCGTCCCAGTATGAGATCA CTTTTTGGGAAAGATAATTCACATCTAAGTAAACAAGCTTCGGTTATTTCACCCTCCGAG ACATTTTCGGAGAACATTAAAAGTTCGTTTGATTTAATTCAGTTTATTCAAAGATATCCG ACCGCCCTGTTTTCTCAAAAGGCTAAATTTTTCTTCTTATCATTAGCTCTTTGTCATAGC TGTCTTCCCAAAAAAACTCATAACGAATCAATAGGAGAAGACTCCATAGAGTATCAGTCT TCATCTCCAGATGAATTAGCACTAGTAACCGCTGCTAGAGACCTTGGTTATATTGTATTG AATAGAAATGCACAGATTTTGACCATTAAAACTTTTCCAGACGGTTTTGACGGTGAAGCA AAATTGGAAAATTATGAAATTTTGAACTATATTGACTTCAATTCTCAAAGAAAGAGAATG TCTGTTCTTGTACGTATGCCAAATCAACCAAATCAAGTTCTTTTAATTTGCAAAGGTGCT GATAACGTTATTATGGAGCGTTTACATGATCGCGAATTGGCTGCCAAAAAAATGGCAGAC ATATGCACCAGTACCAAAGAACGTAAAGACGCAGAAGCAGAGCTGGTTCTCCAGCAAAGA AAGTCTCTGGAAAGAATGGTAGATGAAGAGGCTATGGCCAGGACCTCATTGAGAAATTCT TTGTCTAGCGTACCGAGAGCAAGTTTATCATTGCAGGCAGTGAGAAAAAGTCTTTCAATG AAGAACAGCAGGACCCGTGATCCAGAGAAACAAATTGACTCCATTGATCAATTTCTAGAA ACGGTCAAGAAATCTGATCAGGAGATTGGCAGCGTTGTAAATAAAAGCAGGAAGTCACTC CATAAGCAACAAATAGAAAAATATGGGCCGCGAATTTCGATAGATGGAACACATTTTCCG AACAACAATGTCCCAATAGACACAAGAAAAGAAGGACTACAGCATGATTACGATACAGAA ATTTTGGAGCACATTGGAAGCGATGAGTTAATTTTGAATGAAGAGTATGTTATTGAAAGA ACTTTGCAAGCCATCGATGAGTTTTCGACTGAAGGCTTAAGAACTCTGGTCTATGCTTAC AAATGGATCGATATTGGGCAGTATGAAAATTGGAATAAACGCTATCACCAAGCCAAAACC TCCCTTACTGATAGAAAAATCAAAGTCGATGAGGCAGGTGCAGAAATCGAGGATGGTTTA AATCTGTTAGGGGTAACTGCAATCGAAGACAAGTTACAAGACGGCGTTTCAGAAGCTATA GAAAAGATCAGAAGAGCCGGTATAAAAATGTGGATGCTAACAGGGGATAAAAGGGAAACG GCTATAAATATAGGGTATTCGTGTATGTTAATCAAAGATTATTCTACGGTAGTCATTTTA ACTACTACTGATGAAAATATTATATCAAAGATGAATGCGGTTTCTCAAGAAGTTGATTCC GGAAACATAGCACACTGCGTTGTTGTAATTGACGGTGCCACGATGGCCATGTTTGAAGGA AATCCTACATACATGTCTGTATTTGTTGAGCTTTGCACTAAAACAGACTCGGTAATATGT TGCCGTGCTTCACCTTCTCAAAAAGCCCTTATGGTTTCCAACATAAGAAATACCGATCCA AACTTAGTCACGTTGGCAATAGGTGATGGTGCGAATGATATTGCCATGATACAATCTGCC GATATAGGTGTTGGAATAGCCGGTAAGGAGGGATTGCAAGCATCTAGAGTATCTGATTAT TCTATTGGCCAATTTCGGTTTCTCTTGAAATTGTTATTTGTTCATGGGCGTTATAACTAT ATTCGCACCTCCAAGTTTATGCTTTGCACGTTTTACAAAGAAATTACGTTTTACTTTACG CAGTTGATATATCAAAGATATACAATGTTTTCAGGCTCCTCTCTATATGAGCCATGGTCA TTGTCCATGTTCAATACTCTATTCACATCACTACCGGTTCTATGTATTGGTATGTTTGAG AAAGACTTGAAACCAATGACTCTACTAACAGTCCCTGAATTATATTCGTACGGGCGCCTT TCACAAGGTTTTAATTGGTTAATATTCATGGAGTGGGTTATCTTGGCAACTACGAATTCT CTTATCATTACATTTTTAAATGTTGTAATGTGGGGTATGAGTTCTCTTTCAGATAACACT ATGTATCCTCTAGGTCTCATCAATTTCACAGCCATTGTTGCCTTAATTAACGTTAAATCC CAATTTGTTGAAATGCACAATAGGAATTGGCTGGCTTTTACTTCTGTAGTTCTATCCTGT GGAGGTTGGTTAGTGTGGTGTTGTGCCCTTCCTATTTTGAATAATACTGACCAAATATAT GATGTAGCATATGGCTTTTACAATCATTTTGGTAAAGACATTACATTCTGGTGTACTAGC TTGGTGTTGGCACTGTTACCTATCACTTTAGATATAGTTTACAAAACATTCAAAGTTATG ATATGGCCATCAGATTCTGATATTTTTGCTGAGCTGGAACAAAAAAGTGATATCAGAAAG AAGCTGGAACTAGGAGCCTATAGCGAAATGAGGCAAGGATGGACGTGGGATAAAGATCCC AGTACTTTCACAAGGTACACAGACAAAGTTCTTTCAAGGCCAAGAACTAATTCACGTGCA AGTGCAAAAACTCATAACAGCAGCATATATTCAATGTCAAATGGGAATGTAGACCATTCC TCAAAAAAAAATTTCTTTGGAAACTCTTCTAAAAAGAGCTCTGAACGATACGAGGTATTA CCTAGTGGCAAGTTAATCAAGCGACCATCCTTGAAAACACAAAGCAGTAAAGACAGTATT GGCGGCAATATAACTACTAAACTGACGAAGAAACTGAAGCTTCCATCGAGGAATGTGGAA GATGAAGATGTGAACCAGATTATTCAGGCAAGGCTAAAGGATCTGGAGTAA

Protein Properties

Pfam Domain Function

E1-E2_ATPase (PF00122 )
Hydrolase (PF00702 )

Protein Residues

1656

Protein Molecular Weight

188318.0

Protein Theoretical pI

6.74

Signalling Regions

None

Transmembrane Regions

165-185
452-472
496-516
1158-1178
1319-1339
1366-1386
1396-1416
1433-1453
1474-1494

Protein Sequence

>Probable phospholipid-transporting ATPase DNF3 MGIADGQRRRSSSLRTQMFNKHLYDKYRGRTDDEIELEDINESKTFSGSDNNDKDDRDET SGNYAAEEDYEMEEYGSPDVSYSIITKILDTILDRRRTFHSKDGRHIPIILDHNAIEYKQ AATKRDGHLIDERFNKPYCDNRITSSRYTFYSFLPRQLYAQFSKLANTYFFIVAVLQMIP GWSTTGTYTTIIPLCVFMGISMTREAWDDFRRHRLDKEENNKPVGVLVKDGNNDAQEVYT LPSSVVSSTAYLTKSAAAENNPPLNDDRNSSQGHFLDTHFNNFELLKNKYNVHIHQKKWE KLRVGDFVLLTQDDWVPADLLLLTCDGENSECFVETMALDGETNLKSKQPHPELNKLTKA ASGLANINAQVTVEDPNIDLYNFEGNLELKNHRNDTIMKYPLGPDNVIYRGSILRNTQNV VGMVIFSGEETKIRMNALKNPRTKAPKLQRKINMIIVFMVFVVATISLFSYLGHVLHKKK YIDQNKAWYLFQADAGVAPTIMSFIIMYNTVIPLSLYVTMEIIKVVQSKMMEWDIDMYHA ETNTPCESRTATILEELGQVSYIFSDKTGTLTDNKMIFRKFSLCGSSWLHNVDLGNSEDN FEDNRDNTNSLRLPPKAHNGSSIDVVSIGDQNVLDRLGFSDAPIEKGHRPSLDNFPKSRN SIEYKGNSSAIYTGRPSMRSLFGKDNSHLSKQASVISPSETFSENIKSSFDLIQFIQRYP TALFSQKAKFFFLSLALCHSCLPKKTHNESIGEDSIEYQSSSPDELALVTAARDLGYIVL NRNAQILTIKTFPDGFDGEAKLENYEILNYIDFNSQRKRMSVLVRMPNQPNQVLLICKGA DNVIMERLHDRELAAKKMADICTSTKERKDAEAELVLQQRKSLERMVDEEAMARTSLRNS LSSVPRASLSLQAVRKSLSMKNSRTRDPEKQIDSIDQFLETVKKSDQEIGSVVNKSRKSL HKQQIEKYGPRISIDGTHFPNNNVPIDTRKEGLQHDYDTEILEHIGSDELILNEEYVIER TLQAIDEFSTEGLRTLVYAYKWIDIGQYENWNKRYHQAKTSLTDRKIKVDEAGAEIEDGL NLLGVTAIEDKLQDGVSEAIEKIRRAGIKMWMLTGDKRETAINIGYSCMLIKDYSTVVIL TTTDENIISKMNAVSQEVDSGNIAHCVVVIDGATMAMFEGNPTYMSVFVELCTKTDSVIC CRASPSQKALMVSNIRNTDPNLVTLAIGDGANDIAMIQSADIGVGIAGKEGLQASRVSDY SIGQFRFLLKLLFVHGRYNYIRTSKFMLCTFYKEITFYFTQLIYQRYTMFSGSSLYEPWS LSMFNTLFTSLPVLCIGMFEKDLKPMTLLTVPELYSYGRLSQGFNWLIFMEWVILATTNS LIITFLNVVMWGMSSLSDNTMYPLGLINFTAIVALINVKSQFVEMHNRNWLAFTSVVLSC GGWLVWCCALPILNNTDQIYDVAYGFYNHFGKDITFWCTSLVLALLPITLDIVYKTFKVM IWPSDSDIFAELEQKSDIRKKLELGAYSEMRQGWTWDKDPSTFTRYTDKVLSRPRTNSRA SAKTHNSSIYSMSNGNVDHSSKKNFFGNSSKKSSERYEVLPSGKLIKRPSLKTQSSKDSI GGNITTKLTKKLKLPSRNVEDEDVNQIIQARLKDLE

References

External Links

Resource	Link
Saccharomyces Genome Database	DNF3
Uniprot ID	Q12674
Uniprot Name	ATC8_YEAST
GenBank Gene ID	Z49705
Genebank Protein ID	825567

General Reference

Bowman, S., Churcher, C., Badcock, K., Brown, D., Chillingworth, T., Connor, R., Dedman, K., Devlin, K., Gentles, S., Hamlin, N., Hunt, S., Jagels, K., Lye, G., Moule, S., Odell, C., Pearson, D., Rajandream, M., Rice, P., Skelton, J., Walsh, S., Whitehead, S., Barrell, B. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Nature 387:90-93.9169872
Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258
Chi, A., Huttenhower, C., Geer, L. Y., Coon, J. J., Syka, J. E., Bai, D. L., Shabanowitz, J., Burke, D. J., Troyanskaya, O. G., Hunt, D. F. (2007). "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Proc Natl Acad Sci U S A 104:2193-2198.17287358
Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956