Probable phospholipid-transporting ATPase DNF1 (P32660)

Identification

Name

Probable phospholipid-transporting ATPase DNF1

Synonyms

Not Available

Gene Name

DNF1

Enzyme Class

3.6.3.1

Biological Properties

General Function

Involved in nucleotide binding

Specific Function

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of phospholipids (Potential)

Cellular Location

Membrane; Multi-pass membrane protein

SMPDB Pathways

Lysolipid incorporation into ER	PW002532
Lysolipid incorporation into ER PC(10:0/10:0)	PW002780
Lysolipid incorporation into ER PC(14:0/14:0)	PW002783
Lysolipid incorporation into ER PC(16:0/16:0)	PW002784
Lysolipid incorporation into ER PC(16:1(11Z)/16:1(11Z))	PW002786

KEGG Pathways

Not Available

SMPDB Reactions

Adenosine triphosphate + water + PC(14:0/0:0) → ADP + phosphate + PC(14:0/0:0)

Adenosine triphosphate + water + PE(14:0/0:0) → ADP + phosphate + PE(14:0/0:0)

Adenosine triphosphate + water + Lyso-PS(14:0/0:0) → ADP + phosphate + Lyso-PS(14:0/0:0)

Lyso-PI(14:0/0:0) + Adenosine triphosphate + water → Lyso-PI(14:0/0:0) + ADP + phosphate

Lyso-PS(0:0/14:0) + Adenosine triphosphate + water → Lyso-PS(0:0/14:0) + ADP + phosphate

KEGG Reactions

Not Available

Metabolites

YMDB ID	Name	View
YMDB00109	Adenosine triphosphate	Show
YMDB00890	water	Show
YMDB00907	phosphate	Show
YMDB00914	ADP	Show
YMDB01175	PC(14:0/0:0)	Show
YMDB01194	PE(14:0/0:0)	Show
YMDB13347	Lyso-PI(0:0/14:0)	Show
YMDB13365	Lyso-PS(0:0/14:0)	Show

GO Classification

Component
cell part
membrane
membrane part
intrinsic to membrane
integral to membrane
Function
adenyl nucleotide binding
hydrolase activity, acting on acid anhydrides
adenyl ribonucleotide binding
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
ATP binding
ion binding
cation binding
metal ion binding
substrate-specific transporter activity
transporter activity
lipid transporter activity
transmembrane transporter activity
phospholipid transporter activity
phospholipid-translocating ATPase activity
hydrolase activity
substrate-specific transmembrane transporter activity
ion transmembrane transporter activity
binding
magnesium ion binding
nucleoside binding
ATPase activity, coupled to transmembrane movement of ions
catalytic activity
purine nucleoside binding
ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism
Process
purine nucleoside triphosphate biosynthetic process
purine ribonucleoside triphosphate biosynthetic process
ATP biosynthetic process
nitrogen compound metabolic process
cellular nitrogen compound metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleobase, nucleoside and nucleotide metabolic process
establishment of localization
nucleoside phosphate metabolic process
transport
lipid transport
nucleotide metabolic process
phospholipid transport
purine nucleotide metabolic process
purine nucleotide biosynthetic process
metabolic process

Gene Properties

Chromosome Location

chromosome 5

Locus

YER166W

Gene Sequence

>4716 bp ATGTCTGGAACTTTTCATGGCGATGGGCATGCTCCCATGTCGCCCTTTGAAGATACATTT CAATTTGAAGATAATAGCAGTAATGAAGATACACATATTGCACCTACCCATTTTGATGAT GGTGCAACAAGCAACAAATACAGCCGGCCACAGGTCAGCTTCAATGATGAAACACCCAAA AATAAACGTGAAGATGCAGAAGAATTCACATTTAACGATGACACAGAATATGACAATCAT TCCTTTCAGCCAACACCGAAACTTAATAATGGATCTGGGACGTTTGATGATGTGGAATTA GACAATGATAGTGGTGAACCGCACACTAACTACGATGGTATGAAAAGATTCCGGATGGGT ACAAAAAGGAATAAAAAAGGAAACCCAATAATGGGAAGATCTAAGACCTTGAAATGGGCT AGGAAGAACATTCCAAACCCATTTGAAGACTTTACGAAGGATGATATTGACCCTGGTGCC ATAAATCGTGCGCAAGAACTAAGGACTGTGTATTACAATATGCCCTTACCAAAAGATATG ATAGATGAAGAAGGCAACCCCATTATGCAGTATCCTCGTAATAAAATCAGAACTACCAAA TATACCCCGTTAACATTCTTACCAAAAAACATTCTATTTCAGTTTCATAATTTCGCCAAC GTTTATTTCTTAGTTTTAATAATTTTAGGTGCATTTCAAATTTTTGGTGTCACGAACCCT GGTTTAAGTGCGGTTCCGTTAGTTGTCATTGTTATAATCACTGCGATCAAGGATGCTATT GAAGACTCAAGAAGAACCGTCCTAGATTTAGAGGTGAATAATACCAAGACACATATTTTA GAAGGTGTTGAGAACGAAAATGTCTCAACCGATAATATTTCATTGTGGAGAAGGTTTAAG AAAGCAAACTCTAGACTCCTTTTCAAGTTTATACAATATTGTAAAGAACATTTAACTGAA GAGGGCAAAAAGAAGAGAATGCAGAGAAAGCGTCATGAACTAAGAGTACAAAAAACTGTT GGAACGAGTGGACCCAGATCCTCTCTAGACTCCATTGACAGTTACAGAGTATCTGCAGAT TATGGCCGTCCTTCATTGGATTATGATAATTTGGAACAAGGGGCCGGTGAAGCCAATATA GTTGATAGATCCCTACCACCCAGAACTGATTGTAAATTTGCCAAGAATTATTGGAAAGGT GTCAAGGTTGGTGATATTGTTCGTATCCATAACAATGATGAAATCCCTGCTGACATTATC TTACTTTCAACGTCGGATACAGATGGTGCCTGCTACGTAGAAACTAAAAATTTGGATGGT GAAACAAACTTAAAGGTACGCCAATCCTTGAAGTGTACCAATACTATAAGAACGTCAAAA GATATTGCTAGAACAAAATTCTGGATCGAAAGTGAAGGGCCCCACTCAAATTTGTACACT TATCAAGGTAATATGAAATGGAGAAATCTTGCAGATGGCGAGATACGAAATGAGCCAATT ACTATTAATAACGTTCTGCTTCGTGGTTGCACTCTTAGAAATACTAAGTGGGCAATGGGT GTTGTAATGTTTACCGGTGGTGACACAAAAATCATGTTAAACTCTGGCATTACACCCACC AAGAAGTCCAGGATTTCAAGAGAACTAAACTTCTCTGTGGTGATAAATTTCGTGCTACTT TTCATCTTATGTTTTGTTTCTGGTATTGCAAATGGTGTATATTACGACAAAAAAGGCAGA TCACGGTTTTCCTATGAATTTGGAACAATTGCTGGGTCAGCGGCGACAAACGGTTTCGTC TCATTTTGGGTTGCTGTTATTCTTTACCAATCTTTAGTCCCAATCTCTTTGTACATTTCT GTGGAGATCATCAAAACTGCACAAGCGGCCTTCATTTACGGAGACGTTTTACTCTACAAT GCCAAATTGGATTATCCTTGTACACCAAAATCCTGGAATATTTCTGACGATTTAGGTCAA GTGGAATATATATTTTCTGATAAAACAGGTACATTAACTCAAAATGTAATGGAGTTTAAA AAATGTACTATCAATGGTGTTTCATATGGAAGAGCTTACACCGAAGCGTTAGCGGGATTA AGAAAAAGACAAGGTATCGACGTCGAAACCGAAGGTAGGCGTGAGAAGGCCGAAATTGCC AAGGATAGGGATACAATGATCGATGAACTGAGAGCTTTATCAGGAAATTCACAATTTTAT CCCGAAGAAGTTACCTTTGTATCAAAGGAGTTCGTTCGCGATTTGAAAGGGGCTAGTGGT GAAGTACAACAAAGATGCTGTGAGCACTTTATGTTAGCATTAGCTTTATGTCACTCCGTC CTTGTAGAAGCAAACCCGGACAATCCAAAAAAACTGGATTTAAAAGCTCAATCTCCAGAC GAAGCAGCGTTAGTAGCCACAGCTAGAGATGTAGGATTCAGTTTTGTTGGAAAAACCAAA AAAGGGTTGATAATTGAAATGCAAGGTATTCAAAAAGAATTTGAGATTCTAAATATTTTG GAGTTTAATTCATCGAGAAAAAGAATGTCCTGTATTGTCAAAATTCCGGGTCTGAACCCA GGGGACGAGCCTAGAGCACTTTTAATTTGTAAAGGTGCAGATTCCATTATATACTCAAGA TTAAGTCGCCAGAGTGGTAGCAATAGTGAAGCTATTTTGGAAAAAACTGCTTTGCATTTA GAGCAGTACGCTACTGAAGGTTTAAGAACGTTATGCATAGCTCAAAGGGAGTTAAGTTGG TCTGAATACGAAAAATGGAACGAAAAGTACGATATTGCTGCTGCATCATTAGCAAACAGA GAGGATGAATTAGAAGTTGTTGCAGATTCCATTGAACGCGAATTGATATTATTGGGCGGT ACAGCTATCGAAGATCGTTTACAAGATGGTGTTCCGGATTGTATAGAGTTGCTTGCAGAG GCAGGAATTAAATTATGGGTTCTTACGGGTGATAAAGTTGAAACAGCTATAAACATTGGA TTTTCTTGCAACCTTTTGAATAATGAAATGGAATTGTTAGTTATTAAAACAACTGGTGAT GACGTGAAAGAATTCGGATCAGAACCAAGTGAAATTGTGGATGCTTTACTCTCCAAATAC TTGAAGGAGTATTTCAACTTAACCGGTTCAGAAGAAGAGATTTTTGAAGCTAAAAAGGAT CACGAATTTCCGAAGGGTAATTACGCCATCGTTATCGATGGTGATGCTTTGAAATTGGCG CTATATGGTGAAGATATAAGAAGAAAGTTTCTTTTACTCTGCAAGAATTGTAGAGCTGTC TTATGCTGTAGAGTATCTCCATCTCAAAAAGCAGCCGTTGTTAAGTTAGTCAAGGATTCT CTAGATGTCATGACACTAGCCATCGGCGATGGTTCCAACGACGTCGCCATGATTCAATCT GCGGATGTTGGTATTGGTATTGCCGGTGAAGAAGGTCGACAAGCTGTTATGTGCTCAGAC TACGCCATTGGACAATTCAGATATTTGGCTAGATTGGTCCTCGTTCACGGTAGGTGGTCT TATAAGAGACTGGCAGAAATGATTCCTGAGTTTTTTTACAAAAACATGATTTTTGCTTTG GCATTGTTCTGGTATGGTATCTATAATGATTTTGATGGGTCTTACCTGTATGAATACACA TACATGATGTTCTATAATTTGGCATTTACATCCCTACCAGTCATCTTCTTAGGTATTCTG GACCAGGACGTGAATGACACAATATCATTAGTGGTCCCTCAGTTATACCGTGTTGGAATT TTAAGGAAAGAATGGAACCAAAGAAAGTTCTTATGGTATATGTTGGACGGATTATATCAG TCTATTATCTGTTTTTTTTTCCCTTACTTGGTTTATCACAAAAATATGATAGTTACGAGC AATGGGTTAGGATTGGATCATCGTTATTTTGTCGGTGTTTATGTGACCACTATTGCTGTA ATTTCGTGTAACACATATGTTTTATTACACCAATATAGATGGGATTGGTTTAGTGGATTA TTCATTGCACTGTCTTGCCTCGTAGTTTTTGCATGGACGGGAATATGGTCTTCTGCGATA GCTAGTAGAGAATTTTTCAAAGCTGCAGCGCGTATCTATGGAGCACCTTCATTTTGGGCT GTTTTCTTTGTTGCCGTGCTGTTCTGCTTACTGCCCCGTTTCACATATGACAGTTTTCAA AAATTTTTCTACCCAACTGATGTGGAAATAGTAAGAGAGATGTGGCAACATGGCCATTTC GATCATTATCCTCCTGGTTACGATCCAACAGATCCCAACAGACCCAAGGTCACAAAAGCT GGGCAACACGGAGAGAAGATTATAGAAGGCATAGCACTTTCCGACAACTTAGGAGGATCG AACTATTCAAGGGATAGTGTTGTCACAGAGGAGATTCCAATGACTTTCATGCACGGTGAA GATGGAAGTCCTAGCGGATATCAGAAGCAAGAAACCTGGATGACTTCACCCAAGGAAACA CAAGATCTTCTTCAATCGCCCCAATTTCAGCAGGCCCAAACGTTCGGAAGAGGCCCATCA ACCAACGTCAGGTCATCCTTGGACCGCACAAGAGAACAGATGATCGCCACTAACCAACTA GATAACCGCTACTCTGTGGAAAGAGCGAGAACATCTCTAGATCTACCAGGCGTAACTAAC GCAGCCTCCTTAATCGGAACACAACAGAACAATTAA

Protein Properties

Pfam Domain Function

E1-E2_ATPase (PF00122 )

Protein Residues

1571

Protein Molecular Weight

177796.0

Protein Theoretical pI

5.87

Signalling Regions

None

Transmembrane Regions

215-235
240-260
554-574
595-615
1189-1209
1220-1240
1271-1291
1308-1328
1335-1355
1376-1396

Protein Sequence

>Probable phospholipid-transporting ATPase DNF1 MSGTFHGDGHAPMSPFEDTFQFEDNSSNEDTHIAPTHFDDGATSNKYSRPQVSFNDETPK NKREDAEEFTFNDDTEYDNHSFQPTPKLNNGSGTFDDVELDNDSGEPHTNYDGMKRFRMG TKRNKKGNPIMGRSKTLKWARKNIPNPFEDFTKDDIDPGAINRAQELRTVYYNMPLPKDM IDEEGNPIMQYPRNKIRTTKYTPLTFLPKNILFQFHNFANVYFLVLIILGAFQIFGVTNP GLSAVPLVVIVIITAIKDAIEDSRRTVLDLEVNNTKTHILEGVENENVSTDNISLWRRFK KANSRLLFKFIQYCKEHLTEEGKKKRMQRKRHELRVQKTVGTSGPRSSLDSIDSYRVSAD YGRPSLDYDNLEQGAGEANIVDRSLPPRTDCKFAKNYWKGVKVGDIVRIHNNDEIPADII LLSTSDTDGACYVETKNLDGETNLKVRQSLKCTNTIRTSKDIARTKFWIESEGPHSNLYT YQGNMKWRNLADGEIRNEPITINNVLLRGCTLRNTKWAMGVVMFTGGDTKIMLNSGITPT KKSRISRELNFSVVINFVLLFILCFVSGIANGVYYDKKGRSRFSYEFGTIAGSAATNGFV SFWVAVILYQSLVPISLYISVEIIKTAQAAFIYGDVLLYNAKLDYPCTPKSWNISDDLGQ VEYIFSDKTGTLTQNVMEFKKCTINGVSYGRAYTEALAGLRKRQGIDVETEGRREKAEIA KDRDTMIDELRALSGNSQFYPEEVTFVSKEFVRDLKGASGEVQQRCCEHFMLALALCHSV LVEANPDNPKKLDLKAQSPDEAALVATARDVGFSFVGKTKKGLIIEMQGIQKEFEILNIL EFNSSRKRMSCIVKIPGLNPGDEPRALLICKGADSIIYSRLSRQSGSNSEAILEKTALHL EQYATEGLRTLCIAQRELSWSEYEKWNEKYDIAAASLANREDELEVVADSIERELILLGG TAIEDRLQDGVPDCIELLAEAGIKLWVLTGDKVETAINIGFSCNLLNNEMELLVIKTTGD DVKEFGSEPSEIVDALLSKYLKEYFNLTGSEEEIFEAKKDHEFPKGNYAIVIDGDALKLA LYGEDIRRKFLLLCKNCRAVLCCRVSPSQKAAVVKLVKDSLDVMTLAIGDGSNDVAMIQS ADVGIGIAGEEGRQAVMCSDYAIGQFRYLARLVLVHGRWSYKRLAEMIPEFFYKNMIFAL ALFWYGIYNDFDGSYLYEYTYMMFYNLAFTSLPVIFLGILDQDVNDTISLVVPQLYRVGI LRKEWNQRKFLWYMLDGLYQSIICFFFPYLVYHKNMIVTSNGLGLDHRYFVGVYVTTIAV ISCNTYVLLHQYRWDWFSGLFIALSCLVVFAWTGIWSSAIASREFFKAAARIYGAPSFWA VFFVAVLFCLLPRFTYDSFQKFFYPTDVEIVREMWQHGHFDHYPPGYDPTDPNRPKVTKA GQHGEKIIEGIALSDNLGGSNYSRDSVVTEEIPMTFMHGEDGSPSGYQKQETWMTSPKET QDLLQSPQFQQAQTFGRGPSTNVRSSLDRTREQMIATNQLDNRYSVERARTSLDLPGVTN AASLIGTQQNN

References

External Links

Resource	Link
Saccharomyces Genome Database	DNF1
Uniprot ID	P32660
Uniprot Name	ATC5_YEAST
GenBank Gene ID	U18922
Genebank Protein ID	603407

General Reference

Dietrich, F. S., Mulligan, J., Hennessy, K., Yelton, M. A., Allen, E., Araujo, R., Aviles, E., Berno, A., Brennan, T., Carpenter, J., Chen, E., Cherry, J. M., Chung, E., Duncan, M., Guzman, E., Hartzell, G., Hunicke-Smith, S., Hyman, R. W., Kayser, A., Komp, C., Lashkari, D., Lew, H., Lin, D., Mosedale, D., Davis, R. W., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Nature 387:78-81.9169868
Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258
Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
Chi, A., Huttenhower, C., Geer, L. Y., Coon, J. J., Syka, J. E., Bai, D. L., Shabanowitz, J., Burke, D. J., Troyanskaya, O. G., Hunt, D. F. (2007). "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Proc Natl Acad Sci U S A 104:2193-2198.17287358
Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956