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Putative prephenate dehydratase (P32452)

Identification
NamePutative prephenate dehydratase
Synonyms
  • PDT
  • Phenylalanine-requiring protein 2
Gene NamePHA2
Enzyme Class
Biological Properties
General FunctionInvolved in prephenate dehydratase activity
Specific FunctionPrephenate = phenylpyruvate + H(2)O + CO(2)
Cellular LocationCytoplasm
SMPDB Pathways
Phenylalanine metabolismPW002437 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Phenylalanine metabolismec00360 Map00360
Phenylalanine, tyrosine and tryptophan biosynthesisec00400 Map00400
SMPDB Reactions
Prephenate + hydron → water + Carbon dioxide + keto-phenylpyruvic acid
KEGG Reactions
hydron + Prephenate → Carbon dioxide + water + keto-phenylpyruvic acid
Metabolites
YMDB IDNameView
YMDB00324PrephenateShow
YMDB00786keto-phenylpyruvic acidShow
YMDB00862hydronShow
YMDB00890waterShow
YMDB00912Carbon dioxideShow
GO Classification
Component
Not Available
Function
carbon-oxygen lyase activity
hydro-lyase activity
prephenate dehydratase activity
catalytic activity
lyase activity
Process
cellular amino acid metabolic process
aromatic amino acid family metabolic process
L-phenylalanine metabolic process
L-phenylalanine biosynthetic process
metabolic process
cellular metabolic process
cellular amino acid and derivative metabolic process
Gene Properties
Chromosome Locationchromosome 14
LocusYNL316C
Gene Sequence>YNL316C PHA2 SGDID:S000005260, Chr XIV from 43075-42071, reverse complement, Verified ORF, "Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway" ATGGCCAGCAAGACTTTGAGGGTTCTTTTTCTGGGTCCCAAAGGTACGTATTCCCATCAA GCTGCATTACAACAATTTCAATCAACATCTGATGTTGAGTACCTCCCAGCAGCCTCTATC CCCCAATGTTTTAACCAATTGGAGAACGACACTAGTATAGATTATTCAGTGGTACCGTTG GAAAATTCCACCAATGGACAAGTAGTTTTTTCCTATGATCTCTTGCGTGATAGGATGATC AAAAAAGCCCTATCCTTACCTGCTCCAGCAGATACTAATAGAATTACACCAGATATAGAA GTTATAGCGGAGCAATATGTACCCATTACCCATTGTCTAATCAGCCCAATCCAACTACCA AATGGTATTGCATCCCTTGGAAATTTTGAAGAAGTCATAATACACTCACATCCGCAAGTA TGGGGCCAGGTTGAATGTTACTTAAGGTCCATGGCAGAAAAATTTCCGCAGGTCACCTTT ATAAGATTGGATTGTTCTTCCACATCTGAATCAGTGAACCAATGCATTCGGTCATCAACG GCCGATTGCGACAACATTCTGCATTTAGCCATTGCTAGTGAAACAGCTGCCCAATTGCAT AAGGCGTACATCATTGAACATTCGATAAATGATAAGCTAGGAAATACAACAAGATTTTTA GTATTGAAGAGAAGGGAGAACGCAGGCGACAATGAAGTAGAAGACACTGGATTACTACGG GTTAACCTACTCACCTTTACTACTCGTCAAGATGACCCTGGTTCTTTGGTAGATGTTTTG AACATACTAAAAATCCATTCACTCAACATGTGTTCTATAAACTCTAGACCATTCCATTTG GACGAACATGATAGAAACTGGCGATATTTATTTTTCATTGAATATTACACCGAGAAGAAT ACCCCAAAGAATAAAGAAAAATTCTATGAAGATATCAGCGACAAAAGTAAACAGTGGTGC CTGTGGGGTACATTCCCCAGAAATGAGAGATATTATCACAAATAA
Protein Properties
Pfam Domain Function
Protein Residues334
Protein Molecular Weight38224.80078
Protein Theoretical pI6.13
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Putative prephenate dehydratase MASKTLRVLFLGPKGTYSHQAALQQFQSTSDVEYLPAASIPQCFNQLENDTSIDYSVVPL ENSTNGQVVFSYDLLRDRMIKKALSLPAPADTNRITPDIEVIAEQYVPITHCLISPIQLP NGIASLGNFEEVIIHSHPQVWGQVECYLRSMAEKFPQVTFIRLDCSSTSESVNQCIRSST ADCDNILHLAIASETAAQLHKAYIIEHSINDKLGNTTRFLVLKRRENAGDNEVEDTGLLR VNLLTFTTRQDDPGSLVDVLNILKIHSLNMCSINSRPFHLDEHDRNWRYLFFIEYYTEKN TPKNKEKFYEDISDKSKQWCLWGTFPRNERYYHK
References
External Links
ResourceLink
Saccharomyces Genome Database PHA2
Uniprot IDP32452
Uniprot NamePHA2_YEAST
General Reference
  • Maftahi, M., Nicaud, J. M., Levesque, H., Gaillardin, C. (1995). "Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames." Yeast 11:1077-1085.7502583
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  • Ackerman, S. H., Martin, J., Tzagoloff, A. (1992). "Characterization of ATP11 and detection of the encoded protein in mitochondria of Saccharomyces cerevisiae." J Biol Chem 267:7386-7394.1532796
  • Kellis, M., Patterson, N., Endrizzi, M., Birren, B., Lander, E. S. (2003). "Sequencing and comparison of yeast species to identify genes and regulatory elements." Nature 423:241-254.12748633
  • Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106