Ferrochelatase, mitochondrial (P16622)

Identification
NameFerrochelatase, mitochondrial
Synonyms
  • Heme synthase
  • Protoheme ferro-lyase
Gene NameHEM15
Enzyme Class
Biological Properties
General FunctionInvolved in ferrochelatase activity
Specific FunctionCatalyzes the ferrous insertion into protoporphyrin IX
Cellular LocationMitochondrion inner membrane; Peripheral membrane protein; Matrix side.
SMPDB PathwaysNot Available
KEGG Pathways
Porphyrin and chlorophyll metabolismec00860 Map00860
SMPDB ReactionsNot Available
KEGG Reactions
Iron(2+) + Protoporphyrin IX → Heme + hydron
Metabolites
YMDB IDNameView
YMDB00041HemeShow
YMDB00055Protoporphyrin IXShow
YMDB00379Iron(2+)Show
YMDB00862hydronShow
YMDB00987Protoheme IXShow
GO Classification
Component
Not Available
Function
catalytic activity
lyase activity
ferrochelatase activity
Process
metabolic process
nitrogen compound metabolic process
heme biosynthetic process
tetrapyrrole metabolic process
porphyrin metabolic process
porphyrin biosynthetic process
Gene Properties
Chromosome Locationchromosome 15
LocusYOR176W
Gene Sequence>1182 bp ATGCTTTCCAGAACAATCCGTACACAAGGTTCCTTCCTAAGAAGATCACAACTGACCATT ACAAGATCATTTTCGGTTACATTCAACATGCAGAATGCACAAAAGAGATCACCCACAGGA ATTGTTTTGATGAACATGGGTGGCCCCTCTAAAGTTGAGGAAACATATGATTTTTTGTAT CAATTATTTGCCGATAATGACCTAATTCCCATTAGTGCTAAGTATCAGAAGACAATTGCT AAATATATTGCTAAGTTTCGTACCCCCAAGATAGAGAAGCAATATAGGGAAATTGGTGGG GGCTCCCCAATCCGGAAATGGTCTGAGTATCAAGCCACTGAGGTCTGTAAAATCTTAGAT AAAACCTGTCCAGAAACGGCGCCTCATAAGCCTTACGTGGCGTTTCGTTACGCAAAGCCG CTAACCGCAGAAACTTATAAACAAATGCTAAAAGATGGCGTGAAGAAGGCAGTGGCCTTT TCTCAATATCCTCATTTCTCTTATTCCACTACCGGGTCATCCATCAACGAATTGTGGAGA CAGATTAAGGCATTGGACTCCGAGAGATCTATATCTTGGTCGGTTATTGATCGTTGGCCT ACAAATGAAGGTCTAATCAAGGCCTTCTCCGAAAATATCACCAAAAAACTACAAGAGTTT CCGCAACCTGTCAGAGACAAGGTTGTTTTATTGTTTTCCGCACATTCTCTACCCATGGAT GTTGTTAACACCGGTGATGCCTACCCAGCTGAGGTAGCTGCGACGGTTTACAACATCATG CAAAAATTAAAGTTTAAAAACCCTTATAGGTTGGTTTGGCAATCCCAAGTTGGACCAAAA CCATGGTTGGGAGCGCAGACAGCTGAAATTGCGGAATTTTTAGGCCCCAAAGTTGATGGC CTAATGTTTATTCCTATCGCCTTTACCTCTGATCATATTGAAACATTGCATGAAATTGAC TTAGGCGTCATTGGGGAATCGGAATATAAGGATAAATTTAAGAGATGCGAATCTTTAAAT GGCAACCAGACCTTTATTGAAGGCATGGCAGATCTCGTCAAAAGCCACTTACAGAGTAAC CAACTCTATTCTAATCAACTACCTCTTGATTTTGCACTTGGCAAGTCCAATGATCCTGTA AAGGACCTTTCATTGGTATTTGGCAATCACGAATCTACTTGA
Protein Properties
Pfam Domain Function
Protein Residues393
Protein Molecular Weight44595.80078
Protein Theoretical pI9.46
PDB Fileshow
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Ferrochelatase, mitochondrial MLSRTIRTQGSFLRRSQLTITRSFSVTFNMQNAQKRSPTGIVLMNMGGPSKVEETYDFLY QLFADNDLIPISAKYQKTIAKYIAKFRTPKIEKQYREIGGGSPIRKWSEYQATEVCKILD KTCPETAPHKPYVAFRYAKPLTAETYKQMLKDGVKKAVAFSQYPHFSYSTTGSSINELWR QIKALDSERSISWSVIDRWPTNEGLIKAFSENITKKLQEFPQPVRDKVVLLFSAHSLPMD VVNTGDAYPAEVAATVYNIMQKLKFKNPYRLVWQSQVGPKPWLGAQTAEIAEFLGPKVDG LMFIPIAFTSDHIETLHEIDLGVIGESEYKDKFKRCESLNGNQTFIEGMADLVKSHLQSN QLYSNQLPLDFALGKSNDPVKDLSLVFGNHEST
References
External Links
ResourceLink
Saccharomyces Genome Database HEM15
Uniprot IDP16622
Uniprot NameHEMH_YEAST
GenBank Gene IDJ05395
Genebank Protein ID171660
PDB ID
1LBQ
General Reference
  • Labbe-Bois, R. (1990). "The ferrochelatase from Saccharomyces cerevisiae. Sequence, disruption, and expression of its structural gene HEM15." J Biol Chem 265:7278-7283.2185242
  • Gokhman, I., Zamir, A. (1990). "The nucleotide sequence of the ferrochelatase and tRNA(val) gene region from Saccharomyces cerevisiae." Nucleic Acids Res 18:6130.2235498
  • Dujon, B., Albermann, K., Aldea, M., Alexandraki, D., Ansorge, W., Arino, J., Benes, V., Bohn, C., Bolotin-Fukuhara, M., Bordonne, R., Boyer, J., Camasses, A., Casamayor, A., Casas, C., Cheret, G., Cziepluch, C., Daignan-Fornier, B., Dang, D. V., de Haan, M., Delius, H., Durand, P., Fairhead, C., Feldmann, H., Gaillon, L., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Nature 387:98-102.9169874
  • Camadro, J. M., Labbe, P. (1988). "Purification and properties of ferrochelatase from the yeast Saccharomyces cerevisiae. Evidence for a precursor form of the protein." J Biol Chem 263:11675-11682.3042776
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356