CDP-diacylglycerol--inositol 3-phosphatidyltransferase (P06197)

Identification
NameCDP-diacylglycerol--inositol 3-phosphatidyltransferase
Synonyms
  • Phosphatidylinositol synthase
  • PI synthase
  • PtdIns synthase
Gene NamePIS1
Enzyme Class
Biological Properties
General FunctionInvolved in phosphotransferase activity, for other substituted phosphate groups
Specific FunctionCDP-diacylglycerol + myo-inositol = CMP + phosphatidyl-1D-myo-inositol
Cellular LocationMicrosome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion outer membrane
SMPDB Pathways
Glycerophospholipid metabolismPW002493 ThumbThumb?image type=greyscaleThumb?image type=simple
Inositol phosphate metabolismPW002495 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Glycerophospholipid metabolismec00564 Map00564
Inositol phosphate metabolismec00562 Map00562
SMPDB ReactionsNot Available
KEGG Reactions
Myoinositol + CDP-diacylglycerol → Cytidine monophosphate + 1-Phosphatidyl-D-myo-inositol + hydron
Myoinositol + CDP-diacylglycerol ↔ Cytidine monophosphate + 1-Phosphatidyl-D-myo-inositol + hydron
Metabolites
YMDB IDNameView
YMDB00173MyoinositolShow
YMDB002301-Phosphatidyl-D-myo-inositolShow
YMDB00406Cytidine monophosphateShow
YMDB00862hydronShow
GO Classification
Component
membrane
cell part
Function
transferase activity, transferring phosphorus-containing groups
phosphotransferase activity, for other substituted phosphate groups
catalytic activity
transferase activity
Process
organophosphate metabolic process
phospholipid metabolic process
phospholipid biosynthetic process
metabolic process
Gene Properties
Chromosome Locationchromosome 16
LocusYPR113W
Gene Sequence>663 bp ATGAGTTCGAATTCAACACCAGAAAAGGTTACTGCAGAACACGTTCTGTGGTATATTCCC AATAAGATCGGTTATGTTCGTGTTATCACCGCCGCCCTTTCTTTTTTCGTTATGAAGAAT CATCCTACGGCCTTTACATGGTTGTATAGTACATCATGTCTACTGGATGCGCTAGACGGA ACCATGGCAAGAAAGTACAATCAGGTTTCCAGTCTGGGTGCCGTTCTGGACATGGTTACC GACAGATCCAGTACCGCTGGCTTGATGTGTTTCCTTTGTGTGCAGTATCCCCAATGGTGC GTTTTCTTCCAATTAATGCTGGGCTTGGATATTACTAGTCACTACATGCATATGTATGCC AGTTTAAGTGCTGGTAAGACTTCTCATAAAAGTGTGGGCGAGGGTGAGTCCAGATTGTTA CACCTGTACTACACGAGAAGAGACGTACTGTTCACTATCTGTGCGTTTAACGAACTATTT TATGCTGGATTGTACTTGCAGTTGTTCTCAAATTCTGCAACCTTTGGTAAATGGACTACA ATCATAAGTTTCCCTGGTTACGTGTTCAAGCAGACCGCAAACGTTGTCCAGCTAAAAAGG GCAGCCTTGATTTTAGCAGACAACGATGCCAAGAATGCCAACGAGAAGAACAAGACTTAC TGA
Protein Properties
Pfam Domain Function
Protein Residues220
Protein Molecular Weight24823.5
Protein Theoretical pI8.87
Signalling Regions
  • None
Transmembrane Regions
  • 21-41
  • 46-66
  • 76-96
  • 99-119
  • 146-166
  • 171-191
Protein Sequence>CDP-diacylglycerol--inositol 3-phosphatidyltransferase MSSNSTPEKVTAEHVLWYIPNKIGYVRVITAALSFFVMKNHPTAFTWLYSTSCLLDALDG TMARKYNQVSSLGAVLDMVTDRSSTAGLMCFLCVQYPQWCVFFQLMLGLDITSHYMHMYA SLSAGKTSHKSVGEGESRLLHLYYTRRDVLFTICAFNELFYAGLYLQLFSNSATFGKWTT IISFPGYVFKQTANVVQLKRAALILADNDAKNANEKNKTY
References
External Links
ResourceLink
Saccharomyces Genome Database PIS1
Uniprot IDP06197
Uniprot NamePIS_YEAST
GenBank Gene IDJ02697
Genebank Protein ID172173
General Reference
  • Nikawa, J., Kodaki, T., Yamashita, S. (1987). "Primary structure and disruption of the phosphatidylinositol synthase gene of Saccharomyces cerevisiae." J Biol Chem 262:4876-4881.3031032
  • Bussey, H., Storms, R. K., Ahmed, A., Albermann, K., Allen, E., Ansorge, W., Araujo, R., Aparicio, A., Barrell, B., Badcock, K., Benes, V., Botstein, D., Bowman, S., Bruckner, M., Carpenter, J., Cherry, J. M., Chung, E., Churcher, C., Coster, F., Davis, K., Davis, R. W., Dietrich, F. S., Delius, H., DiPaolo, T., Hani, J., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Nature 387:103-105.9169875
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258