Ornithine carbamoyltransferase (P05150)

Identification
NameOrnithine carbamoyltransferase
Synonyms
  • Ornithine transcarbamylase
  • OTCase
Gene NameARG3
Enzyme Class
Biological Properties
General FunctionInvolved in carboxyl- or carbamoyltransferase activity
Specific FunctionCarbamoyl phosphate + L-ornithine = phosphate + L-citrulline
Cellular LocationCytoplasm
SMPDB PathwaysNot Available
KEGG Pathways
Arginine and proline metabolismec00330 Map00330
SMPDB ReactionsNot Available
KEGG Reactions
Ornithine + Carbamoylphosphate → Citrulline + phosphate + hydron
Metabolites
YMDB IDNameView
YMDB00060CitrullineShow
YMDB00082CarbamoylphosphateShow
YMDB00353OrnithineShow
YMDB00862hydronShow
YMDB00907phosphateShow
GO Classification
Component
macromolecular complex
protein complex
ornithine carbamoyltransferase complex
Function
transferase activity, transferring one-carbon groups
binding
carboxylic acid binding
amino acid binding
carboxyl- or carbamoyltransferase activity
ornithine carbamoyltransferase activity
catalytic activity
transferase activity
Process
metabolic process
cellular metabolic process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
Gene Properties
Chromosome Locationchromosome 10
LocusYJL088W
Gene Sequence>1017 bp ATGTCAACCACAGCATCCACGCCTTCATCTTTACGTCATTTGATTTCTATAAAAGATCTT TCTGATGAAGAATTCAGAATCTTAGTACAAAGAGCTCAACATTTCAAGAATGTTTTTAAA GCAAATAAAACGAATGATTTCCAATCCAACCATCTGAAACTATTGGGTAGAACTATAGCC TTAATATTTACTAAAAGATCAACTAGAACGAGAATTTCGACCGAAGGTGCAGCCACCTTC TTTGGTGCCCAACCGATGTTTTTAGGTAAAGAGGATATTCAGCTTGGTGTCAATGAATCA TTTTACGATACCACCAAGGTTGTATCATCTATGGTTTCATGTATTTTTGCCCGTGTGAAC AAACATGAAGACATACTTGCTTTTTGCAAGGATTCCTCTGTACCGATCATCAACTCTCTA TGTGACAAATTCCACCCTTTGCAAGCAATTTGTGATCTTTTAACAATAATCGAAAACTTC AATATATCTCTAGATGAAGTAAATAAGGGAATCAATTCAAAATTGAAGATGGCATGGATT GGTGATGCCAATAATGTCATAAATGATATGTGCATCGCATGTCTGAAATTCGGTATAAGT GTCAGTATTTCCACTCCCCCCGGTATTGAAATGGATTCCGATATTGTCGATGAAGCAAAG AAAGTTGCTGAGAGAAACGGTGCGACATTTGAATTAACACACGACTCTTTAAAGGCCTCC ACCAATGCCAATATATTAGTAACCGATACTTTCGTTTCCATGGGTGAAGAATTTGCGAAA CAGGCCAAGCTGAAACAATTCAAAGGTTTTCAAATCAATCAAGAACTTGTCTCTGTGGCT GATCCAAACTACAAATTTATGCATTGTCTGCCAAGACATCAAGAAGAAGTTAGTGATGAT GTCTTTTATGGAGAGCATTCCATAGTCTTTGAAGAAGCAGAAAACAGATTATATGCAGCT ATGTCTGCCATTGATATCTTTGTTAATAATAAAGGTAATTTCAAGGACTTGAAATAA
Protein Properties
Pfam Domain Function
Protein Residues338
Protein Molecular Weight37844.80078
Protein Theoretical pI6.24
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Ornithine carbamoyltransferase MSTTASTPSSLRHLISIKDLSDEEFRILVQRAQHFKNVFKANKTNDFQSNHLKLLGRTIA LIFTKRSTRTRISTEGAATFFGAQPMFLGKEDIQLGVNESFYDTTKVVSSMVSCIFARVN KHEDILAFCKDSSVPIINSLCDKFHPLQAICDLLTIIENFNISLDEVNKGINSKLKMAWI GDANNVINDMCIACLKFGISVSISTPPGIEMDSDIVDEAKKVAERNGATFELTHDSLKAS TNANILVTDTFVSMGEEFAKQAKLKQFKGFQINQELVSVADPNYKFMHCLPRHQEEVSDD VFYGEHSIVFEEAENRLYAAMSAIDIFVNNKGNFKDLK
References
External Links
ResourceLink
Saccharomyces Genome Database ARG3
Uniprot IDP05150
Uniprot NameOTC_YEAST
GenBank Gene IDM11946
Genebank Protein ID171075
General Reference
  • Huygen, R., Crabeel, M., Glansdorff, N. (1987). "Nucleotide sequence of the ARG3 gene of the yeast Saccharomyces cerevisiae encoding ornithine carbamoyltransferase. Comparison with other carbamoyltransferases." Eur J Biochem 166:371-377.3038540
  • Miosga, T., Schaaff-Gerstenschlager, I., Chalwatzis, N., Baur, A., Boles, E., Fournier, C., Schmitt, S., Velten, C., Wilhelm, N., Zimmermann, F. K. (1995). "Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(II)2-Cys6 binuclear cluster domain and a putative alpha 2-SCB-alpha 2 binding site." Yeast 11:681-689.7483841
  • Galibert, F., Alexandraki, D., Baur, A., Boles, E., Chalwatzis, N., Chuat, J. C., Coster, F., Cziepluch, C., De Haan, M., Domdey, H., Durand, P., Entian, K. D., Gatius, M., Goffeau, A., Grivell, L. A., Hennemann, A., Herbert, C. J., Heumann, K., Hilger, F., Hollenberg, C. P., Huang, M. E., Jacq, C., Jauniaux, J. C., Katsoulou, C., Karpfinger-Hartl, L., et, a. l. .. (1996). "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." EMBO J 15:2031-2049.8641269
  • Crabeel, M., Huygen, R., Verschueren, K., Messenguy, F., Tinel, K., Cunin, R., Glansdorff, N. (1985). "General amino acid control and specific arginine repression in Saccharomyces cerevisiae: physical study of the bifunctional regulatory region of the ARG3 gene." Mol Cell Biol 5:3139-3148.3915770
  • El Alami, M., Dubois, E., Oudjama, Y., Tricot, C., Wouters, J., Stalon, V., Messenguy, F. (2003). "Yeast epiarginase regulation, an enzyme-enzyme activity control: identification of residues of ornithine carbamoyltransferase and arginase responsible for enzyme catalytic and regulatory activities." J Biol Chem 278:21550-21558.12679340
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106