FAD-linked sulfhydryl oxidase ERV2 (Q12284)

Identification
NameFAD-linked sulfhydryl oxidase ERV2
SynonymsNot Available
Gene NameERV2
Enzyme Class
Biological Properties
General FunctionInvolved in thiol oxidase activity
Specific FunctionFAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation in the endoplasmic reticulum lumen in parallel to ERO1
Cellular LocationEndoplasmic reticulum membrane; Single-pass type III membrane protein; Lumenal side
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00888Hydrogen peroxideShow
YMDB00900oxygenShow
GO Classification
Component
Not Available
Function
oxidoreductase activity, acting on a sulfur group of donors
oxidoreductase activity, acting on a sulfur group of donors, oxygen as acceptor
thiol oxidase activity
catalytic activity
oxidoreductase activity
Process
oxidation reduction
metabolic process
Gene Properties
Chromosome Locationchromosome 16
LocusYPR037C
Gene Sequence>591 bp ATGAAACAGATAGTCAAAAGAAGCCATGCCATCAGAATAGTTGCAGCATTAGGAATCATA GGCCTGTGGATGTTTTTCTCGTCTAATGAACTATCCATCGCTACGCCGGGCCTAATCAAG GCGAAGTCTGGTATAGATGAAGTGCAAGGGGCGGCTGCTGAGAAGAACGACGCTCGGTTG AAAGAGATCGAGAAGCAAACCATTATGCCATTGATGGGCGATGACAAGGTGAAGAAGGAA GTGGGCAGGGCGTCGTGGAAGTACTTCCATACCCTGCTGGCCCGTTTTCCGGACGAGCCT ACTCCTGAAGAAAGAGAGAAACTGCACACGTTTATTGGGTTGTATGCAGAACTCTATCCA TGCGGGGAATGTTCATATCACTTTGTAAAGTTGATTGAGAAGTATCCCGTACAGACATCT AGCAGGACGGCTGCCGCAATGTGGGGATGCCACATTCACAACAAGGTGAACGAATACCTA AAGAAAGACATATATGACTGTGCTACCATCCTGGAGGACTACGATTGTGGATGTAGTGAC AGCGACGGTAAACGCGTGTCTCTCGAGAAGGAGGCTAAACAGCACGGTTGA
Protein Properties
Pfam Domain Function
Protein Residues196
Protein Molecular Weight22141.30078
Protein Theoretical pI7.5
PDB Fileshow
Signalling Regions
  • None
Transmembrane Regions
  • 13-35
Protein Sequence>FAD-linked sulfhydryl oxidase ERV2 MKQIVKRSHAIRIVAALGIIGLWMFFSSNELSIATPGLIKAKSGIDEVQGAAAEKNDARL KEIEKQTIMPLMGDDKVKKEVGRASWKYFHTLLARFPDEPTPEEREKLHTFIGLYAELYP CGECSYHFVKLIEKYPVQTSSRTAAAMWGCHIHNKVNEYLKKDIYDCATILEDYDCGCSD SDGKRVSLEKEAKQHG
References
External Links
ResourceLink
Saccharomyces Genome Database ERV2
Uniprot IDQ12284
Uniprot NameERV2_YEAST
GenBank Gene IDZ68111
Genebank Protein ID1072405
PDB ID
1JRA
General Reference
  • Bussey, H., Storms, R. K., Ahmed, A., Albermann, K., Allen, E., Ansorge, W., Araujo, R., Aparicio, A., Barrell, B., Badcock, K., Benes, V., Botstein, D., Bowman, S., Bruckner, M., Carpenter, J., Cherry, J. M., Chung, E., Churcher, C., Coster, F., Davis, K., Davis, R. W., Dietrich, F. S., Delius, H., DiPaolo, T., Hani, J., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Nature 387:103-105.9169875
  • Stein, G., Lisowsky, T. (1998). "Functional comparison of the yeast scERV1 and scERV2 genes." Yeast 14:171-180.9483805
  • Gerber, J., Muhlenhoff, U., Hofhaus, G., Lill, R., Lisowsky, T. (2001). "Yeast ERV2p is the first microsomal FAD-linked sulfhydryl oxidase of the Erv1p/Alrp protein family." J Biol Chem 276:23486-23491.11313344
  • Sevier, C. S., Cuozzo, J. W., Vala, A., Aslund, F., Kaiser, C. A. (2001). "A flavoprotein oxidase defines a new endoplasmic reticulum pathway for biosynthetic disulphide bond formation." Nat Cell Biol 3:874-882.11584268
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Gross, E., Sevier, C. S., Vala, A., Kaiser, C. A., Fass, D. (2002). "A new FAD-binding fold and intersubunit disulfide shuttle in the thiol oxidase Erv2p." Nat Struct Biol 9:61-67.11740506