tRNA guanosine-2'-O-methyltransferase TRM3 (Q07527)

Identification
NametRNA guanosine-2'-O-methyltransferase TRM3
Synonyms
  • tRNA [Gm18] methyltransferase
  • tRNA methylase 3
Gene NameTRM3
Enzyme Class
Biological Properties
General FunctionInvolved in RNA binding
Specific FunctionSpecifically methylates guanosine-18 in various tRNAs
Cellular LocationCytoplasm
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00198S-AdenosylhomocysteineShow
YMDB00297S-AdenosylmethionineShow
GO Classification
Component
Not Available
Function
RNA methyltransferase activity
catalytic activity
transferase activity
transferase activity, transferring one-carbon groups
methyltransferase activity
binding
nucleic acid binding
RNA binding
Process
metabolic process
macromolecule metabolic process
cellular macromolecule metabolic process
RNA metabolic process
RNA processing
Gene Properties
Chromosome Locationchromosome 4
LocusYDL112W
Gene Sequence>4311 bp ATGGTTGGAGGTGCTCTCATCTGCAAATATTTACCACGCGAAGAGCAACTAAAGCTTATA TCAGACTTGATTCAGAATGACAGCTTAGAAGAGGTGCTGGAGTTGATTGAAACTTCGCCA CTGGATATAACGACGGACAGCAACATTGAAACACCAATCTTTGAAAAAATTACTGAGCAG GTCATTGCGTATGCTAGTATAGACGGTGAAGCCAGGGAAATGTTTCGCTCTTCGAGGGCT GAAATGAACAAGGCGCTAAGAACTTCTGCACAATTACTATGTTGTTTACCCAGTGTATGG CATAAATTTCAGGTGTGGATGAGCTACCGGTTAAATGACATCATTAGCGAAAACTATAAA CATCTCTTCAATGACAACTTTGGAAAAAAGATTGTACAACCATTTTTCGATTCTTTTGCT GAGGAACAAAACGCTAATATCAAGCATGAAAATCTGCACTTAGATATCCTTAGCCTGCTT CACTACTTAGAAGTTGTATATTTATTTGACGAATGTAAGAATGGTATATCGTCCAAATGC CTAGATTTTATCATTGTTCCATTGTTAGGCTGTAATTCGGAAGAGATTGCTGATTCCTGC TCCAAGTTAATGAGATGGCATATCAAATATTTATCGAAATGCTGCAATACCGATAGTAAC TTTGATAAGCTCATTTGGACATTTATCAAACAATTGTACGCTGAAGGCTCCCAACAAGCT TGGAAGCAAAAGAACAGTTTATCTTTTCTCCTAAGATTCCTTTTAGCAGCAGAGTTGTCT CCTGAACTTATTACTTATATAAAGACCGATGCTTATTGGAGGCACATTCAAACAGAGCTG GATAATGATGTGCACGAACACAGAAAATTGGCACTCTCAATTCTTAAATTAACAATTCAA AAACTGTCATCTCATGGTATAACATTGCAAACAACATTTTATAAATGCAATGATCTAGCA AATATCGAGATGCTGGGTAGCTGGAAGAAATTCACCACCTTATATGAAATGATTGCTCTG GACACATCATTGAATCAAATACAAGCGGCCAAACAAGATATAATAAAAATTTTTGATAAC GAGCATTTACACCATAGTTGGGGCCTTATCTTGCTTTCTACGGGATTGAAATCATCAATG GAGAGTGTCAGAAAATACATGATGACGTTAATGTTCTCGATTACAAATATGTCAGCATTC TCTTCAAACTTGCCATTATTGACAAAAACTCTTCTTCCAGCTGCAATGTCCGCTCATTAT TTTGATGTAAAAGGTGTCAGTTGTCCCCATGGTGAAAAACTTTCATTATTTGTCAATAAC TTACTTTCCCAAACAACAGAAGGCATATCAGATATATTATTTGAAATTTTGAAATTATTG GTGGAAAAAGGGACCTCTTTTGACCCTTCTAGGATATACTTGTCCTACGGTATCTTAGTT TTCTTCCAGAATAACAAACAGAAAACAATCAACTCTGACCATTTGAGTCTGATCAGGAAA TTGTACGATTTTGCAGCCGAAGAAGAGGTTCTCGAAACTACAATCCAAACAATTTACTTG AAGTTCTTACTGTACATTGATCCATCTGTCTCCGCATCTGAATTATTGTTTACATTAGTT TCACACATTAAGTTAAAGGGAGGGACATACAAATATGTTGAGCCTCTTTTTGAAGATTAT AGGGATCTTGCCGTTTCTCACTTTGACGATTTGCAAGCGAAAGAAAACTTGACGACAAAC ATCGGAAAGGATACTATTTTTGACCTCTTAGCATCTATCATCTTCGATTTCAAGGATATT GATATTACTCCCGATTTTTTAATTGAAGTTGCGAAGTCGAAGCAAGATATACCCGTTTAC ACTTCAAAGGCAGTTACTTTTCTAACTCAGCTACTTTCAGGCGAACCTTCGAACGGTTAT ACCTATGAAAATGCAACCGCTTTGTTGTCTTACCCAAATTTTACAATATCTACATGGAAA TCCATCAATGTGAATAATTTATTTAAGTCGGTAATGGAAAAATTTTCGCTTGATAAATTC AAGTTCTTTGCTGAAATTTACCAGAAGACTTATGAATGTAGGTTCGATACAATCGAGCTA AATTTTAATGACCTTCTGAGCCTATATGAAATGGTTAAAAAGTCTGCCAATCAGTGTTCA AGAGAGAGCTTTAAAGTGAAGGACAGCGCTTATTCTTCTTACTTTGAACTTTTAAATACC TTCCTGAAAACATACGCCTTAAATCGTGACTCTTCTGAAGGAAATGATGATGAGCTTCAC ATACTACTACGCTTAGTTGACGAGAATATTAACAAAGATAATGGGAATTATCTGGGGAAC CTGGCAGTCTGTAAATTATTGTACTTTATCATAGATTCCTACATCCATTGCTCCACATCT GTTTCTGATGATGATATTTTCATAGTGAAATTCATTTTTGAGAAATTTTCGTTTATATGG GAATGTATTAATAGTGAAAGATTAGTTTTGAAGGAAAGGGATCTGCATTTGATGCTAATT AAGGGATTATTTCATCCTGTGATCCTGTACTTTGGATCAAATCAGTATATCGATACTTTG ACCTCGAAGTTGGAGGAACATGCACAAACGATTATATCATTGAGTTATTCCAGAAGAAGC CTACTCCCTCTTCTCGGATCACAACTCAGAGTATTTATGAAGTTTTATGGGAAATTGTTA AGAGAAGATGTCAACTATTGGTGGTTGATTAACATTATAGTAGGTGTTTTCAAACAGCCG CAAATGGATGTCAACCTTTACAAGCTCAAACCTGTTATTTCCAGCCTATTTGACCACAAA CTGAACAATTATTACATAAAAGGCGATGAACTTTATGAAAAGGTGTACGGACCCGACGAA ATATTGGCTAGAGTATCTATTATTGATTCTATTTTATATGCAAATGATCAGTTGAAAATT CGATTAATCGAAAAAGTTACTGAAAAAACAAACGCACTTTATGCTATAAAGAGAACCGAC GGTGCCGAAGCGTTACAGAGGTTGTTGCAGTGGCAATTGTTATTATTATCACTACTAACC ACCAATGAAAAAAAATTAAGCGAAACTAGCATGATCAGAATTCTAAAGAGTATCGAGGAT GAAAGTTCACCACTAGTAAGAGTATATAAAGAATGGTTCATTTCCTCAAAGGTTGTTGAT TATTATAAAACTGGCAATCCAAAATTTGCTGAGGATTATCTCTTCTCATTGCTGGAGGAT CATAGCAAACCGGTGTTTGTAGTTAGCGCAGAAAAAATATGCTTTATGGTGTTGAAAGAT TTAAGAAACGACGAAAAGAAATATGGATTTACACAGTTACTAGACAGATTCATTTGCACG TTAGTTCCTAACGCAGCTTCAAACAAACCTCTGGTAAGGCATTTTTCTAATTCTCTAATA ATTTCATTATGGCCTACATTCAAAGCCTACCTATCGGATCACACACTGAGAAACATCATT GAGAACTTATATTCCAATGCCAAAAAGACTCAAATATTTGGTCAATATCGTGCCGGGGAT GCCAACATATGGGATCTGAAGGGTGATAGGAAATTAACGAACATGTTTGGTGGTGTGCTC AAAAAAGTTACCGACCATGATTGCCCGTACATTTCTGAGTCTGTTTTCGAAAAATATTTG CAGGAGAAAGATATTGTTCCTATAGGTACCGATGAAAGGTCCTTATGGCTTGATAAAAGA GACACCAATACGGAATCTGTTAACAACGCGAACATCTCCTGTGACACTTCTCCACTGCAA ACAAAAAGTGGAGCTTGGGAAACCGTTCTGGATCTGGACAATAAAAAATCGAATGATGTC GTTACACGTTCTGAATTGATTGTGGTATCTTCATTAGTTGATAAGCCGCCAAACCTGGGA GGTATTTGTAGGTTATGTGATGTTTTAGGTGTGGGACTGCTTACTGTACAAGACATCAAA GTCAAAAACCATCCTCAATTTAAAAATGTTGCTGTGACTGCTGATAGATGGATGCCCATG GAGGAAGTTGCCCTAGATGAGATTGCAAGTTTCATGAAAGAGAAAAAGAAGGAAGGGTAC ACATTAATTGGGCTAGAGCAAACGGATAAATCAGTGAAGCTAGACAACAATTTCCAATTC CCTAAAAAATCATTGATCTTGCTAGGAACCGAAGCATTCGGTATTCCGGGGACTTTGTTA AGCGAATTGGATTTATGTTTAGAGATTCAACAATTTGGTGTGATCAGATCAATGAATATT CAAACGGCAACCGCAGTTATCGTTCATTCCTATACAGTTCAACACATGTAA
Protein Properties
Pfam Domain Function
Protein Residues1436
Protein Molecular Weight165046.0
Protein Theoretical pI5.96
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>tRNA guanosine-2'-O-methyltransferase TRM3 MVGGALICKYLPREEQLKLISDLIQNDSLEEVLELIETSPLDITTDSNIETPIFEKITEQ VIAYASIDGEAREMFRSSRAEMNKALRTSAQLLCCLPSVWHKFQVWMSYRLNDIISENYK HLFNDNFGKKIVQPFFDSFAEEQNANIKHENLHLDILSLLHYLEVVYLFDECKNGISSKC LDFIIVPLLGCNSEEIADSCSKLMRWHIKYLSKCCNTDSNFDKLIWTFIKQLYAEGSQQA WKQKNSLSFLLRFLLAAELSPELITYIKTDAYWRHIQTELDNDVHEHRKLALSILKLTIQ KLSSHGITLQTTFYKCNDLANIEMLGSWKKFTTLYEMIALDTSLNQIQAAKQDIIKIFDN EHLHHSWGLILLSTGLKSSMESVRKYMMTLMFSITNMSAFSSNLPLLTKTLLPAAMSAHY FDVKGVSCPHGEKLSLFVNNLLSQTTEGISDILFEILKLLVEKGTSFDPSRIYLSYGILV FFQNNKQKTINSDHLSLIRKLYDFAAEEEVLETTIQTIYLKFLLYIDPSVSASELLFTLV SHIKLKGGTYKYVEPLFEDYRDLAVSHFDDLQAKENLTTNIGKDTIFDLLASIIFDFKDI DITPDFLIEVAKSKQDIPVYTSKAVTFLTQLLSGEPSNGYTYENATALLSYPNFTISTWK SINVNNLFKSVMEKFSLDKFKFFAEIYQKTYECRFDTIELNFNDLLSLYEMVKKSANQCS RESFKVKDSAYSSYFELLNTFLKTYALNRDSSEGNDDELHILLRLVDENINKDNGNYLGN LAVCKLLYFIIDSYIHCSTSVSDDDIFIVKFIFEKFSFIWECINSERLVLKERDLHLMLI KGLFHPVILYFGSNQYIDTLTSKLEEHAQTIISLSYSRRSLLPLLGSQLRVFMKFYGKLL REDVNYWWLINIIVGVFKQPQMDVNLYKLKPVISSLFDHKLNNYYIKGDELYEKVYGPDE ILARVSIIDSILYANDQLKIRLIEKVTEKTNALYAIKRTDGAEALQRLLQWQLLLLSLLT TNEKKLSETSMIRILKSIEDESSPLVRVYKEWFISSKVVDYYKTGNPKFAEDYLFSLLED HSKPVFVVSAEKICFMVLKDLRNDEKKYGFTQLLDRFICTLVPNAASNKPLVRHFSNSLI ISLWPTFKAYLSDHTLRNIIENLYSNAKKTQIFGQYRAGDANIWDLKGDRKLTNMFGGVL KKVTDHDCPYISESVFEKYLQEKDIVPIGTDERSLWLDKRDTNTESVNNANISCDTSPLQ TKSGAWETVLDLDNKKSNDVVTRSELIVVSSLVDKPPNLGGICRLCDVLGVGLLTVQDIK VKNHPQFKNVAVTADRWMPMEEVALDEIASFMKEKKKEGYTLIGLEQTDKSVKLDNNFQF PKKSLILLGTEAFGIPGTLLSELDLCLEIQQFGVIRSMNIQTATAVIVHSYTVQHM
References
External Links
ResourceLink
Saccharomyces Genome Database TRM3
Uniprot IDQ07527
Uniprot NameTRM3_YEAST
GenBank Gene IDZ74160
Genebank Protein ID1431162
General Reference
  • Jacq, C., Alt-Morbe, J., Andre, B., Arnold, W., Bahr, A., Ballesta, J. P., Bargues, M., Baron, L., Becker, A., Biteau, N., Blocker, H., Blugeon, C., Boskovic, J., Brandt, P., Bruckner, M., Buitrago, M. J., Coster, F., Delaveau, T., del Rey, F., Dujon, B., Eide, L. G., Garcia-Cantalejo, J. M., Goffeau, A., Gomez-Peris, A., Zaccaria, P., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Nature 387:75-78.9169867
  • Boskovic, J., Soler-Mira, A., Garcia-Cantalejo, J. M., Ballesta, J. P., Jimenez, A., Remacha, M. (1996). "The sequence of a 16,691 bp segment of Saccharomyces cerevisiae chromosome IV identifies the DUN1, PMT1, PMT5, SRP14 and DPR1 genes, and five new open reading frames." Yeast 12:1377-1384.8923743
  • Cavaille, J., Chetouani, F., Bachellerie, J. P. (1999). "The yeast Saccharomyces cerevisiae YDL112w ORF encodes the putative 2'-O-ribose methyltransferase catalyzing the formation of Gm18 in tRNAs." RNA 5:66-81.9917067
  • Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106