Probable cation-transporting ATPase 1 (P39986)

Identification

Name

Probable cation-transporting ATPase 1

Synonyms

Not Available

Gene Name

SPF1

Enzyme Class

3.6.3.-

Biological Properties

General Function

Involved in nucleotide binding

Specific Function

ATP + H(2)O = ADP + phosphate

Cellular Location

Membrane; Multi-pass membrane protein

SMPDB Pathways

Not Available

KEGG Pathways

Not Available

SMPDB Reactions

Not Available

KEGG Reactions

Not Available

Metabolites

YMDB ID	Name	View
YMDB00109	Adenosine triphosphate	Show
YMDB00907	phosphate	Show
YMDB00914	ADP	Show

GO Classification

Component
cell part
membrane
membrane part
intrinsic to membrane
integral to membrane
Function
ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism
binding
hydrolase activity, acting on acid anhydrides
nucleoside binding
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
catalytic activity
purine nucleoside binding
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
transporter activity
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
transmembrane transporter activity
pyrophosphatase activity
nucleoside-triphosphatase activity
ATPase activity
hydrolase activity
substrate-specific transmembrane transporter activity
ion transmembrane transporter activity
ATPase activity, coupled to transmembrane movement of ions
Process
purine nucleotide biosynthetic process
cation transport
metabolic process
purine nucleoside triphosphate biosynthetic process
purine ribonucleoside triphosphate biosynthetic process
ATP biosynthetic process
establishment of localization
nitrogen compound metabolic process
transport
cellular nitrogen compound metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleoside phosphate metabolic process
ion transport
nucleotide metabolic process
purine nucleotide metabolic process

Gene Properties

Chromosome Location

chromosome 5

Locus

YEL031W

Gene Sequence

>3648 bp ATGACAAAGAAATCATTTGTGAGCAGTCCTATTGTTAGGGACTCGACATTGCTGGTACCA AAGTCATTAATAGCAAAGCCGTACGTATTACCGTTTTTCCCGTTATACGCTACCTTTGCC CAACTATATTTCCAGCAATATGATCGTTACATCAAAGGACCTGAGTGGACTTTTGTCTAC TTGGGGACATTGGTATCCTTGAACATCCTAGTCATGTTAATGCCTGCTTGGAATGTCAAG ATCAAGGCGAAATTCAACTACAGTACTACTAAAAATGTCAATGAAGCTACTCACATTCTC ATCTACACCACTCCAAATAATGGTTCTGATGGTATCGTTGAAATTCAGAGAGTAACTGAA GCGGGCTCTTTGCAGACTTTTTTCCAATTTCAAAAGAAAAGATTCTTGTGGCATGAAAAT GAGCAGGTATTTTCTTCACCAAAGTTCCTTGTTGATGAATCCCCAAAGATCGGTGATTTC CAAAAATGTAAGGGCCATTCCGGTGACCTAACTCATTTGAAGAGACTATATGGTGAGAAT AGTTTTGATATTCCTATTCCAACATTTATGGAATTATTCAAAGAGCACGCTGTTGCGCCA TTGTTTGTTTTCCAAGTTTTCTGTGTTGCCTTATGGCTATTGGATGAGTTCTGGTATTAT TCTTTGTTTAACTTATTCATGATTATCTCCATGGAGGCAGCTGCAGTATTTCAAAGATTA ACTGCCTTGAAGGAATTTAGAACTATGGGTATCAAACCTTATACCATTAACGTGTTTAGA AACAAAAAATGGGTAGCATTGCAAACCAATGAATTGTTACCTATGGATTTAGTGTCTATA ACAAGAACCGCCGAGGAGAGCGCAATTCCTTGCGATTTGATCTTGCTCGATGGCTCCGCT ATTGTTAATGAAGCAATGCTTTCAGGTGAATCTACTCCCTTGTTAAAGGAATCCATTAAA TTACGTCCTAGTGAAGATAATTTACAACTGGACGGTGTTGACAAGATTGCTGTGTTACAT GGTGGTACAAAGGCATTACAAGTAACACCTCCTGAACATAAATCGGATATCCCTCCTCCA CCTGATGGAGGTGCCTTAGCCATCGTCACCAAAACTGGCTTTGAAACATCTCAGGGTTCT TTAGTTCGAGTTATGATTTACTCCGCTGAACGTGTTTCCGTCGATAATAAGGAAGCGTTG ATGTTCATTCTTTTCTTGTTGATTTTCGCTGTTATTGCATCCTGGTACGTTTGGGTTGAG GGTACAAAAATGGGTAGAATTCAATCCAAATTGATCTTGGACTGTATATTAATTATTACA TCTGTTGTTCCTCCTGAGTTACCAATGGAATTGACCATGGCTGTCAACAGTTCTCTGGCC GCCCTTGCCAAGTTCTATGTCTATTGTACTGAACCATTTAGAATTCCATTTGCTGGTAGA ATTGATGTTTGTTGTTTCGATAAAACTGGTACTTTGACCGGCGAAGATCTTGTGTTTGAA GGTCTAGCTGGTATCTCTGCGGACTCCGAAAACATTCGTCACTTGTACAGCGCTGCCGAA GCACCAGAAAGTACAATTCTTGTCATTGGTGCTGCTCACGCTTTAGTTAAATTGGAAGAT GGTGATATTGTTGGTGACCCAATGGAAAAGGCGACCTTAAAGGCTGTTGGTTGGGCTGTC GAAAGAAAAAACAGTAACTATAGAGAGGGCACCGGAAAATTGGATATTATCCGTAGATTT CAATTTTCTTCTGCTTTAAAAAGGTCTGCTTCTATTGCTTCTCACAACGATGCCTTGTTT GCTGCTGTCAAAGGTGCACCTGAAACGATTCGTGAAAGGCTTTCTGACATTCCCAAGAAT TATGACGAAATCTATAAATCCTTTACACGCTCTGGTTCAAGAGTTTTGGCTTTGGCTTCT AAGTCGTTGCCAAAAATGTCTCAGTCTAAAATTGATGATCTAAACCGTGATGATGTTGAA TCTGAATTAACCTTTAATGGGTTTTTGATTTTCCATTGTCCTCTGAAGGATGACGCTATT GAAACCATTAAAATGTTAAATGAATCCTCCCATCGTTCTATTATGATTACTGGTGATAAT CCATTAACTGCTGTTCATGTGGCTAAAGAAGTTGGCATAGTTTTTGGCGAAACTTTAATC TTAGACAGAGCAGGTAAATCTGACGACAATCAGTTGCTGTTCCGTGATGTTGAAGAGACA GTTTCCATCCCATTTGATCCATCAAAAGACACTTTTGATCATTCTAAATTATTTGATAGA TATGATATTGCTGTTACTGGATATGCTTTGAATGCCTTGGAAGGGCATTCTCAATTAAGG GATCTATTACGTCACACCTGGGTTTACGCACGTGTTTCACCATCCCAAAAGGAGTTCTTG CTGAATACACTAAAGGATATGGGATATCAAACTTTAATGTGCGGTGATGGTACTAATGAT GTTGGTGCTTTGAAACAAGCTCATGTTGGTATTGCATTGTTGAATGGTACCGAAGAAGGT TTGAAAAAATTGGGTGAACAACGCAGATTGGAAGGTATGAAAATGATGTATATAAAGCAA ACTGAATTTATGGCTAGATGGAATCAACCACAGCCACCTGTTCCTGAACCGATTGCACAC TTATTCCCACCTGGTCCAAAAAATCCTCATTATCTGAAAGCTTTAGAAAGCAAAGGTACC GTCATTACGCCAGAAATCAGAAAAGCAGTTGAAGAAGCAAATTCCAAACCTGTTGAAGTC ATCAAACCAAATGGACTATCGGAAAAAAAACCTGCTGACTTAGCTTCTTTGTTGTTGAAC TCTGCTGGTGATGCCCAAGGTGATGAAGCACCAGCTCTAAAGTTAGGAGATGCATCTTGC GCTGCCCCTTTTACCTCGAAACTGGCCAATGTTTCTGCTGTTACTAACATTATACGTCAA GGCCGTTGTGCTTTAGTTAACACAATTCAAATGTACAAAATCTTGGCCTTGAATTGTTTA ATCAGTGCCTACTCTTTGTCCATCATTTACATGGCTGGTGTCAAATTTGGTGATGGTCAA GCCACTGTCTCCGGTTTGTTACTATCGGTTTGTTTCTTGAGTATTTCCCGTGGTAAACCT TTAGAAAAACTATCCAAGCAAAGGCCACAGTCCGGAATTTTCAATGTTTACATTATGGGA TCTATTTTGTCTCAATTTGCCGTTCATATTGCCACTTTAGTTTACATCACTACAGAGATT TATAAATTGGAACCCAGAGAGCCACAAGTTGATCTTGAAAAGGAATTTGCTCCATCATTA TTGAATACGGGTATTTTCATCATTCAGTTGGTACAACAAGTCTCTACATTTGCTGTCAAT TATCAAGGTGAGCCATTCAGAGAAAACATTAGGAGTAACAAAGGTATGTATTATGGTTTA TTGGGTGTGACAGGTTTGGCACTTGCAAGTGCTACTGAATTTTTACCTGAGCTAAACGAA GCCATGAAGTTTGTGCCAATGACTGATGACTTTAAGATTAAGTTGACCCTAACATTATTG CTCGATTTCTTTGGTAGTTGGGGTGTCGAGCATTTTTTCAAGTTTTTCTTCATGGACGAC AAACCATCAGACATTTCTGTGCAACAGGTCAAGATTGCCTCTAAATGA

Protein Properties

Pfam Domain Function

E1-E2_ATPase (PF00122 )
Hydrolase (PF00702 )

Protein Residues

1215

Protein Molecular Weight

135267.0

Protein Theoretical pI

6.56

Signalling Regions

None

Transmembrane Regions

22-43
50-72
192-214
218-236
400-419
433-454
994-1013
1021-1037
1056-1079
1100-1122
1134-1153
1171-1193

Protein Sequence

>Probable cation-transporting ATPase 1 MTKKSFVSSPIVRDSTLLVPKSLIAKPYVLPFFPLYATFAQLYFQQYDRYIKGPEWTFVY LGTLVSLNILVMLMPAWNVKIKAKFNYSTTKNVNEATHILIYTTPNNGSDGIVEIQRVTE AGSLQTFFQFQKKRFLWHENEQVFSSPKFLVDESPKIGDFQKCKGHSGDLTHLKRLYGEN SFDIPIPTFMELFKEHAVAPLFVFQVFCVALWLLDEFWYYSLFNLFMIISMEAAAVFQRL TALKEFRTMGIKPYTINVFRNKKWVALQTNELLPMDLVSITRTAEESAIPCDLILLDGSA IVNEAMLSGESTPLLKESIKLRPSEDNLQLDGVDKIAVLHGGTKALQVTPPEHKSDIPPP PDGGALAIVTKTGFETSQGSLVRVMIYSAERVSVDNKEALMFILFLLIFAVIASWYVWVE GTKMGRIQSKLILDCILIITSVVPPELPMELTMAVNSSLAALAKFYVYCTEPFRIPFAGR IDVCCFDKTGTLTGEDLVFEGLAGISADSENIRHLYSAAEAPESTILVIGAAHALVKLED GDIVGDPMEKATLKAVGWAVERKNSNYREGTGKLDIIRRFQFSSALKRSASIASHNDALF AAVKGAPETIRERLSDIPKNYDEIYKSFTRSGSRVLALASKSLPKMSQSKIDDLNRDDVE SELTFNGFLIFHCPLKDDAIETIKMLNESSHRSIMITGDNPLTAVHVAKEVGIVFGETLI LDRAGKSDDNQLLFRDVEETVSIPFDPSKDTFDHSKLFDRYDIAVTGYALNALEGHSQLR DLLRHTWVYARVSPSQKEFLLNTLKDMGYQTLMCGDGTNDVGALKQAHVGIALLNGTEEG LKKLGEQRRLEGMKMMYIKQTEFMARWNQPQPPVPEPIAHLFPPGPKNPHYLKALESKGT VITPEIRKAVEEANSKPVEVIKPNGLSEKKPADLASLLLNSAGDAQGDEAPALKLGDASC AAPFTSKLANVSAVTNIIRQGRCALVNTIQMYKILALNCLISAYSLSIIYMAGVKFGDGQ ATVSGLLLSVCFLSISRGKPLEKLSKQRPQSGIFNVYIMGSILSQFAVHIATLVYITTEI YKLEPREPQVDLEKEFAPSLLNTGIFIIQLVQQVSTFAVNYQGEPFRENIRSNKGMYYGL LGVTGLALASATEFLPELNEAMKFVPMTDDFKIKLTLTLLLDFFGSWGVEHFFKFFFMDD KPSDISVQQVKIASK

References

External Links

Resource	Link
Saccharomyces Genome Database	SPF1
Uniprot ID	P39986
Uniprot Name	ATC6_YEAST
GenBank Gene ID	U18530
Genebank Protein ID	602398

General Reference

Dietrich, F. S., Mulligan, J., Hennessy, K., Yelton, M. A., Allen, E., Araujo, R., Aviles, E., Berno, A., Brennan, T., Carpenter, J., Chen, E., Cherry, J. M., Chung, E., Duncan, M., Guzman, E., Hartzell, G., Hunicke-Smith, S., Hyman, R. W., Kayser, A., Komp, C., Lashkari, D., Lew, H., Lin, D., Mosedale, D., Davis, R. W., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Nature 387:78-81.9169868
Suzuki, C., Shimma, Y. I. (1999). "P-type ATPase spf1 mutants show a novel resistance mechanism for the killer toxin SMKT." Mol Microbiol 32:813-823.10361284
Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258
Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956