You are using an unsupported browser. Please upgrade your browser to a newer version to get the best experience on Yeast Metabolome Database.
Identification |
---|
Name | Phospholipase D1 |
---|
Synonyms | - PLD 1
- Choline phosphatase 1
- Meiosis-specific sporulation-specific protein 14
- Phosphatidylcholine-hydrolyzing phospholipase D1
|
---|
Gene Name | SPO14 |
---|
Enzyme Class | |
---|
Biological Properties |
---|
General Function | Involved in protein binding |
---|
Specific Function | Required for meiosis and spore formation. Seems to be involved in the coordinate induction of late meiotic events. PLD activity is induced under sporulation conditions and seems to be necessary to complete the meiotic cycle, but not for vegetative cell growth |
---|
Cellular Location | Not Available |
---|
SMPDB Pathways | |
---|
KEGG Pathways | |
---|
SMPDB Reactions | |
---|
KEGG Reactions | |
---|
Metabolites | YMDB ID | Name | View |
---|
YMDB00227 | Choline | Show | YMDB00862 | hydron | Show | YMDB00890 | water | Show | YMDB01161 | PA(16:0/16:0) | Show | YMDB01180 | PC(16:0/16:0) | Show |
|
---|
GO Classification | Component |
---|
Not Available | Function |
---|
catalytic activity | binding | protein binding | lipid binding | phospholipid binding | phosphoinositide binding | Process |
---|
metabolic process | cellular process | cell communication |
|
---|
Gene Properties |
---|
Chromosome Location | chromosome 11 |
---|
Locus | YKR031C |
---|
Gene Sequence | >5052 bp
ATGTCTAACGTGTCAACAGCGTCTGGAACTCACTTCGCTCCACCTCAGGCAGATAGATCG
GTGACTGAAGAAGTAGATAGAGTAAATTCGAGACCAGATGAGTTAGAAAACCAAGAAGTA
TTGCGGCAATTACCTGAGAATGGCAATCTGACTAGCAGTTTACAAAGAGAAAAACGAAGA
ACTCCCAACGGCAAGGAGGCGGAGAGGAAGCATGCATTGCCCAAATCCTTTGTAGATAGA
AACCTGAGTGATGTGTCACCGAACCACTCATTAGACCATATTATGCACTCTAATGAACAT
GATCCTCGTCGAGGTTCTGATGAGGAAAATATGCACCGGCTCTACAACAATCTCCATAGC
AGTAACAATAATGTTCATTCTAAGCGGAATTCTAAAAGAGAGGAAGAAAGAGCCCCTCAA
AGGCGTTCTTCTTCTGTAGCTTACACCCAACAGCAGTTCAATGGCTGGAAGAAAGAGTTT
GGCCACGCTTTTAAGAAAATCTCTGCTATTGGTAGACTGAAATCCAGCGTAAATTCTCCT
ACACCGGCTGGAAGCGGCCATCGTCATAACCAGCACCAGCATCAACAAGTAAACGAAGAA
GATTTGTACACACAAAGACTAGCCTCTGATTTATTAGATTCGTTATTGGCAGGATGCCCG
GCCTCATTGTTCGCAAGCACGCAATTTCTGAGAGACGAACATGGGAAGAGAAGGGCTCCT
CTGCTATTAGCTAAACTAGATGTAAGAGTGTCTCCCTTGAAAAATGACAACAACATTTTG
GATATTACCAATAGCAACCATAACCATCGTGGTAACAACAACAACAATACTGGCGAGAAT
TCTGATAGGAGACCCTCTATTCCAAGGAGCTCCAGCATTATAAGCATTTCTTCGAATGTG
GCGGAATTTATGTATAGCAGGAACGAAAACTCTTTATTTAGAATACACTTGGAATATGGG
ATTGATGAAGACAGGCTCAAATGGTCTATTATAAGAAGTTATAAAGATATCAAATCACTA
CATCACAAATTGAAAATCGTCGCGTTCCAACAACTCACTATAAGCAAGCTTTATAGTGAT
AACAACAGATATCATTCGTTACAACTTCCGCATTTCCCCCATTATAAAGAAATGGTTAAA
GAAAGAAATGTAATGGAGAAAAAAGCAGAAAATAAACCATCCTCTGCAGCCTCAGCTCCT
CATACATCGGAAAATAACAATAACGATAATGGCAGCAACATTACATCGTTAGAAACTTTA
TCATCTTCTGAAATATCTGAGTTTAATATAGATAACGTCAAAATGAAACATTTACAAGAC
TTAATTGACGAACCTGACGATTTCTCCCAACCGATACATTTGAGGCTAGAAAGGTATTTG
AGACTACTGAACATTGCATTATGTTTGAGACCTCATGCAAATAGATTGTTTGAATTTTAC
GAATTATCTCCCTTAGGCAATTTATTAAGTCGTGAAAGTGGGTTCCAGGGCAAACAGGGG
TATTTAGTAATTAGATCTACTGCCAAGGCCCAAGGTTGGAGAGTTTCTCATTTTGGTAAA
CATGCGTTTAAAGACATGATCGACAGACATACTACCAAATGGTTCCTGGTAAGAAATTCT
TACCTAACTTACGTGTCGGATCTTTCTTCGACAACTCCTTTAGACGTATTCTTAATAGAT
TGGAAATTTAAGGTTAGATTTTCTGGGAACAAGAATAACATACTTGATAACGAAAACGAG
ATCAACTGGATCATTCATGATCCCAATTTAGAAATAAATGACGAACTAGAAGAGTTCGGT
ATTGAAAATGATGCCAATAATATCCTAGACAAGAATGGAAAATCGAAAACACACCAAAAG
AAGTCCAATATATCTTCAAAACTATTATTATTGACCCTAGAAAACAGTGAAAGGAAACTG
AAAATTATTTGCAAATCAGAGAGTTCGTTAAAGCAATGGATGAGTTCAATTATCAAAATG
TCTACCTCAACACCTTGGTCGAAACCAAATAGATTTGGTAGTTTCGCACCTGTAAGAACA
AATTCGTTTTGCAAATTTTTGGTAGATGGGCGTGATTATTTTTGGTCGTTAAGCGAGGCC
CTCTTAATGGCAAAGGATGTCATATATATCCATGATTGGTGGCTATCCCCTGAATTATAC
TTGCGACGTCCGGTGAAAGGCAACCAGGGATTTAGAATTGATCGTATGCTGAAAAGTTGT
GCAGAAAAAGGTATCAAAATTTTTATTGTGATTTATAGGAATGTGGGGAATATTGTTGGG
ACAGATAGTCTTTGGACCAAGCATTCTATGCTAAATTTACATCCAAATATACATATCATA
AGGTCTCCCAATCAATGGCTACAAAATACATATTTTTGGGCACACCACGAGAAGTTTGTC
GTTATTGATGAAACATTTGCATTTATTGGTGGTACAGATTTATGTTATGGTAGATATGAT
ACTTTTGAACACGTTCTACGAGATGATGCAGAATCTTTATTAGATCAAAATTTTCCTGGT
AAGGATTATTCAAATGCCAGAATTGCTGATTTTCATGATTTAGATAAACCATTCGAATCA
ATGTATGATAGAAAAGTCATTCCGAGGATGCCATGGCATGATGTGCAAATGATGACTTTG
GGTGAGCCAGCAAGAGACTTAGCCCGACATTTTGTTCAAAGATGGAATTACTTATTGAGA
GCTAAGAGACCTAGTAGGTTAACTCCATTACTAACCCCGCCTTCAGATTTAACTGCTGAA
GAATTGAAGAGTTTGCCGATGTTCGAAATTCTACGCGAAAAATCGACTTGTGAAACTCAA
ATACTTAGAAGTGCTGGGAACTGGTCTCTTGGGCTAAAGGAAACGGAATGCTCAATTCAA
AATGCTTACCTGAAGTTAATTGAACAAAGTGAACACTTTATTTATATTGAGAATCAATTC
TTCATTACATCAACTGTCTGGAACGGGACTTGCGTTTTGAATAAAATTGGTGATGCTCTC
GTTGATAGGATAGTCAAAGCTAATCAGGAGAAAAAACCTTGGAAAGCCTTTATTCTTATC
CCACTAATGCCAGGATTCGATTCTCCAGTAGATACTGCAGAAGCCTCAAGTCTTCGTTTA
ATTATGCAATTCCAATATCAATCAATTTCAAGAGGTGAACATTCTACATTTAGTAAACTG
AAGAAACTAAATATTGACCCAGCTCAATATATTCAGTTCTTTTCACTAAGAAAATGGTCG
ACTTTTGCGCCTAATGAAAGATTAATCACCGAGCAGCTCTACGTTCATGCTAAAATTCTG
ATTGCTGATGACAGAAGATGTATTATAGGGTCTGCTAATATTAACGAAAGATCACAACTG
GGTAATAGAGATAGTGAGGTGGCAATTCTTATTAGAGACACCGACTTGATAAAGACCAAA
ATGAATGGTGACGATTACTACGCGGGAAAATTTCCTTGGGAATTAAGGCAGCGATTAATG
AGGGAACATTTAGGTTGTGATGTAGATCTTGTTGAGTTTGTTGAAAAGAAATTTGAAAGG
TTTGAAAAGTTTGCTGCAAAGAATTATGAAAAACTTCATACCCTAAGTAAGGAGGGAGAT
AGTGGTAACAATTGGAGCGATCGAGAAATGATCGATTCCGCTATGATTGAGCTAGGTTAT
CGTGAAATATTTGGTTGTAAATTTAGCCCTCAATGGAAGAGTGGTCATGGAAACAGTGTT
GATGATGGAAGCACTCAGTGTGGCATAAATGAAAAAGAAGTGGGAAGAGAAGATGAAAAC
GTTTATGAAAAATTCTTCAATTCTGTGGACTACGGTAAATCATCACGTAAGAGAACGCCT
CTGCCCAAACATAATTTTGCATCTTTAGGGCTAACATTCAACCACAGGGCAGGAATTGAA
AATGTCGGTATACGAGACCACAAAGTCTTAAGTACTGATCCAAGATTAAGAAAGAATGAT
GAACATAAAAAAGAGGTTGATGGCTACGGTCCCGATTGTTGGAAAAAGGAATCCAACAAA
AAATTCAAGGCCGATGCAACTGAACAGCTAAAGGAGTGGGCATTGAACTCATTGGCCTCG
AAAGTTCTTGACGATAAAGAAATGATAAAATCAGAAATTCCAGAAGGATTTTCAAATTAT
TTGCCCAATGAGAAAGATCTAGAAATGTATCTCACGGATAAAACTGTTACCAATAGAAAC
AAATGGAGTATGCTCAAAAGGATATGCTACTTGCAATATTTATCTCATAAACTCGACGAA
AGAAAAACACAGAGATTGAAGAAAATCAAAGATATGAGAAGACATTTAAGTTCAAGCACA
GAAAGTACCAGAAATGGGTCTAATAGTCTGCCATTAAACGAAAAGAGCAATGAAGGAGAA
AGCACTAATGTCGACCAAGATATCGAAGGCGATGAATATCATAGATTGCACGAAGATATT
TTGAAAAATCAAGAACTAGATGATGGTTCACTGGACGATCTACTTTCTCAGATAATTCCT
AAGATCACTAACTTTAACAGTGGTGAAATTGATGACGCAAAAAAAGAAGAGTTATTGAAA
CTCAACTTCATTGACCCGTATTCCTTCGAGGATCCTCTTATTTCTTCGTTTTCTGAAGGG
CTATGGTTCACTATAGCCTTGAGAAATACATTACTCTATAAATTAGTGTTTCATTGTCAA
CCCGATAATGCTGTACAAAATTGGAAAGAATATGGAGAATTTACTGAATTAGAACAAGAA
TTTCAAATAAACCAGGAAAAGTTGATTGACTTAGAGGCTGAAAATATTAATTCTACTACT
ACAAATGTTGTAGATAAGGATAGGGAAAAGGAAAAAATGAGAAAAGCCGCTGAATTGAGA
ATGAAGTTATCCGGTAGTTTGCTTTATGGATTCAATCAAAAGGTTTTTGACAAACATACG
GCGCAGAGAATCTTGGAAAGGATCCACGGTCATTTGGTCATTTTCCCAACCGAATGGTTA
GCTAAAGAAGTTGAGAGTAGAAATTGGATTTTTAACTCGGATAGACTTTCTCCAATGGAA
ATCTACAATTAA |
---|
Protein Properties |
---|
Pfam Domain Function | |
---|
Protein Residues | 1683 |
---|
Protein Molecular Weight | 195202.0 |
---|
Protein Theoretical pI | 7.63 |
---|
Signalling Regions | |
---|
Transmembrane Regions | |
---|
Protein Sequence | >Phospholipase D1
MSNVSTASGTHFAPPQADRSVTEEVDRVNSRPDELENQEVLRQLPENGNLTSSLQREKRR
TPNGKEAERKHALPKSFVDRNLSDVSPNHSLDHIMHSNEHDPRRGSDEENMHRLYNNLHS
SNNNVHSKRNSKREEERAPQRRSSSVAYTQQQFNGWKKEFGHAFKKISAIGRLKSSVNSP
TPAGSGHRHNQHQHQQVNEEDLYTQRLASDLLDSLLAGCPASLFASTQFLRDEHGKRRAP
LLLAKLDVRVSPLKNDNNILDITNSNHNHRGNNNNNTGENSDRRPSIPRSSSIISISSNV
AEFMYSRNENSLFRIHLEYGIDEDRLKWSIIRSYKDIKSLHHKLKIVAFQQLTISKLYSD
NNRYHSLQLPHFPHYKEMVKERNVMEKKAENKPSSAASAPHTSENNNNDNGSNITSLETL
SSSEISEFNIDNVKMKHLQDLIDEPDDFSQPIHLRLERYLRLLNIALCLRPHANRLFEFY
ELSPLGNLLSRESGFQGKQGYLVIRSTAKAQGWRVSHFGKHAFKDMIDRHTTKWFLVRNS
YLTYVSDLSSTTPLDVFLIDWKFKVRFSGNKNNILDNENEINWIIHDPNLEINDELEEFG
IENDANNILDKNGKSKTHQKKSNISSKLLLLTLENSERKLKIICKSESSLKQWMSSIIKM
STSTPWSKPNRFGSFAPVRTNSFCKFLVDGRDYFWSLSEALLMAKDVIYIHDWWLSPELY
LRRPVKGNQGFRIDRMLKSCAEKGIKIFIVIYRNVGNIVGTDSLWTKHSMLNLHPNIHII
RSPNQWLQNTYFWAHHEKFVVIDETFAFIGGTDLCYGRYDTFEHVLRDDAESLLDQNFPG
KDYSNARIADFHDLDKPFESMYDRKVIPRMPWHDVQMMTLGEPARDLARHFVQRWNYLLR
AKRPSRLTPLLTPPSDLTAEELKSLPMFEILREKSTCETQILRSAGNWSLGLKETECSIQ
NAYLKLIEQSEHFIYIENQFFITSTVWNGTCVLNKIGDALVDRIVKANQEKKPWKAFILI
PLMPGFDSPVDTAEASSLRLIMQFQYQSISRGEHSTFSKLKKLNIDPAQYIQFFSLRKWS
TFAPNERLITEQLYVHAKILIADDRRCIIGSANINERSQLGNRDSEVAILIRDTDLIKTK
MNGDDYYAGKFPWELRQRLMREHLGCDVDLVEFVEKKFERFEKFAAKNYEKLHTLSKEGD
SGNNWSDREMIDSAMIELGYREIFGCKFSPQWKSGHGNSVDDGSTQCGINEKEVGREDEN
VYEKFFNSVDYGKSSRKRTPLPKHNFASLGLTFNHRAGIENVGIRDHKVLSTDPRLRKND
EHKKEVDGYGPDCWKKESNKKFKADATEQLKEWALNSLASKVLDDKEMIKSEIPEGFSNY
LPNEKDLEMYLTDKTVTNRNKWSMLKRICYLQYLSHKLDERKTQRLKKIKDMRRHLSSST
ESTRNGSNSLPLNEKSNEGESTNVDQDIEGDEYHRLHEDILKNQELDDGSLDDLLSQIIP
KITNFNSGEIDDAKKEELLKLNFIDPYSFEDPLISSFSEGLWFTIALRNTLLYKLVFHCQ
PDNAVQNWKEYGEFTELEQEFQINQEKLIDLEAENINSTTTNVVDKDREKEKMRKAAELR
MKLSGSLLYGFNQKVFDKHTAQRILERIHGHLVIFPTEWLAKEVESRNWIFNSDRLSPME
IYN |
---|
References |
---|
External Links | |
---|
General Reference | - Honigberg, S. M., Conicella, C., Espositio, R. E. (1992). "Commitment to meiosis in Saccharomyces cerevisiae: involvement of the SPO14 gene." Genetics 130:703-716.1582554
- Dujon, B., Alexandraki, D., Andre, B., Ansorge, W., Baladron, V., Ballesta, J. P., Banrevi, A., Bolle, P. A., Bolotin-Fukuhara, M., Bossier, P., et, a. l. .. (1994). "Complete DNA sequence of yeast chromosome XI." Nature 369:371-378.8196765
- Rose, K., Rudge, S. A., Frohman, M. A., Morris, A. J., Engebrecht, J. (1995). "Phospholipase D signaling is essential for meiosis." Proc Natl Acad Sci U S A 92:12151-12155.8618862
- Waksman, M., Eli, Y., Liscovitch, M., Gerst, J. E. (1996). "Identification and characterization of a gene encoding phospholipase D activity in yeast." J Biol Chem 271:2361-2364.8576189
- Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
- Gruhler, A., Olsen, J. V., Mohammed, S., Mortensen, P., Faergeman, N. J., Mann, M., Jensen, O. N. (2005). "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Mol Cell Proteomics 4:310-327.15665377
- Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956
|
---|