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Mitochondrial FAD-linked sulfhydryl oxidase ERV1 (P27882)

Identification
NameMitochondrial FAD-linked sulfhydryl oxidase ERV1
Synonyms
  • 14 kDa regulatory protein
  • Essential for respiration and vegetative growth protein 1
Gene NameERV1
Enzyme Class
Biological Properties
General FunctionInvolved in thiol oxidase activity
Specific FunctionFAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. Required for the import and folding of small cysteine-containing proteins in the mitochondrial intermembrane space (IMS). Forms a redox cycle with MIA40 that involves a disulfide relay system. Important for maintaining the cysteine residues in MIA40 in an oxidized state. Reduced ERV1 is reoxidized by cytochrome c. Required for the maturation of cytoplasmic, but not of mitochondrial Fe/S proteins
Cellular LocationMitochondrion intermembrane space
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00888Hydrogen peroxideShow
YMDB00900oxygenShow
GO Classification
Component
Not Available
Function
catalytic activity
oxidoreductase activity
oxidoreductase activity, acting on a sulfur group of donors
oxidoreductase activity, acting on a sulfur group of donors, oxygen as acceptor
thiol oxidase activity
Process
metabolic process
oxidation reduction
Gene Properties
Chromosome Locationchromosome 7
LocusYGR029W
Gene Sequence>570 bp ATGAAAGCAATAGATAAAATGACGGATAATCCACCACAAGAAGGCTTAAGTGGGAGGAAA ATAATATATGACGAAGATGGCAAACCTTGCCGATCATGTAACACCCTACTTGACTTTCAG TACGTGACCGGGAAGATATCTAATGGCCTGAAGAACCTCTCATCTAACGGTAAACTAGCA GGTACGGGGGCTCTCACTGGCGAAGCTTCAGAGTTGATGCCTGGCTCAAGAACATACAGG AAGGTTGACCCTCCTGACGTAGAGCAACTAGGTAGATCTTCATGGACGCTGTTACACTCT GTAGCTGCCAGCTATCCTGCTCAACCTACAGACCAACAGAAGGGTGAAATGAAACAGTTC TTGAATATCTTCTCACATATTTATCCTTGCAACTGGTGTGCTAAAGACTTTGAAAAATAT ATCAGAGAAAATGCACCACAAGTTGAGTCAAGAGAAGAACTTGGGAGGTGGATGTGTGAA GCCCACAATAAAGTCAATAAGAAATTGAGGAAGCCCAAATTTGACTGTAATTTCTGGGAA AAAAGATGGAAGGACGGCTGGGACGAATAA
Protein Properties
Pfam Domain Function
Protein Residues189
Protein Molecular Weight21639.30078
Protein Theoretical pI8.36
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Mitochondrial FAD-linked sulfhydryl oxidase ERV1 MKAIDKMTDNPPQEGLSGRKIIYDEDGKPCRSCNTLLDFQYVTGKISNGLKNLSSNGKLA GTGALTGEASELMPGSRTYRKVDPPDVEQLGRSSWTLLHSVAASYPAQPTDQQKGEMKQF LNIFSHIYPCNWCAKDFEKYIRENAPQVESREELGRWMCEAHNKVNKKLRKPKFDCNFWE KRWKDGWDE
References
External Links
ResourceLink
Saccharomyces Genome Database ERV1
Uniprot IDP27882
Uniprot NameERV1_YEAST
GenBank Gene IDZ72814
Genebank Protein ID1945314
General Reference
  • Lisowsky, T. (1992). "Dual function of a new nuclear gene for oxidative phosphorylation and vegetative growth in yeast." Mol Gen Genet 232:58-64.1552903
  • Nakai, M., Endo, T., Hase, T., Matsubara, H. (1993). "Intramitochondrial protein sorting. Isolation and characterization of the yeast MSP1 gene which belongs to a novel family of putative ATPases." J Biol Chem 268:24262-24269.8226973
  • Rieger, M., Bruckner, M., Schafer, M., Muller-Auer, S. (1997). "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII." Yeast 13:1077-1090.9290212
  • Tettelin, H., Agostoni Carbone, M. L., Albermann, K., Albers, M., Arroyo, J., Backes, U., Barreiros, T., Bertani, I., Bjourson, A. J., Bruckner, M., Bruschi, C. V., Carignani, G., Castagnoli, L., Cerdan, E., Clemente, M. L., Coblenz, A., Coglievina, M., Coissac, E., Defoor, E., Del Bino, S., Delius, H., Delneri, D., de Wergifosse, P., Dujon, B., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Nature 387:81-84.9169869
  • Lisowsky, T. (1996). "Removal of an intron with unique 3' branch site creates an amino-terminal protein sequence directing the scERV1 gene product to mitochondria." Yeast 12:1501-1510.8972573
  • Lee, J., Hofhaus, G., Lisowsky, T. (2000). "Erv1p from Saccharomyces cerevisiae is a FAD-linked sulfhydryl oxidase." FEBS Lett 477:62-66.10899311
  • Hofhaus, G., Lee, J. E., Tews, I., Rosenberg, B., Lisowsky, T. (2003). "The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre." Eur J Biochem 270:1528-1535.12654008
  • Lange, H., Lisowsky, T., Gerber, J., Muhlenhoff, U., Kispal, G., Lill, R. (2001). "An essential function of the mitochondrial sulfhydryl oxidase Erv1p/ALR in the maturation of cytosolic Fe/S proteins." EMBO Rep 2:715-720.11493598
  • Sickmann, A., Reinders, J., Wagner, Y., Joppich, C., Zahedi, R., Meyer, H. E., Schonfisch, B., Perschil, I., Chacinska, A., Guiard, B., Rehling, P., Pfanner, N., Meisinger, C. (2003). "The proteome of Saccharomyces cerevisiae mitochondria." Proc Natl Acad Sci U S A 100:13207-13212.14576278
  • Mesecke, N., Terziyska, N., Kozany, C., Baumann, F., Neupert, W., Hell, K., Herrmann, J. M. (2005). "A disulfide relay system in the intermembrane space of mitochondria that mediates protein import." Cell 121:1059-1069.15989955
  • Rissler, M., Wiedemann, N., Pfannschmidt, S., Gabriel, K., Guiard, B., Pfanner, N., Chacinska, A. (2005). "The essential mitochondrial protein Erv1 cooperates with Mia40 in biogenesis of intermembrane space proteins." J Mol Biol 353:485-492.16181637
  • Allen, S., Balabanidou, V., Sideris, D. P., Lisowsky, T., Tokatlidis, K. (2005). "Erv1 mediates the Mia40-dependent protein import pathway and provides a functional link to the respiratory chain by shuttling electrons to cytochrome c." J Mol Biol 353:937-944.16185707