AdoMet-dependent rRNA methyltransferase SPB1 (P25582)

Identification

Name

AdoMet-dependent rRNA methyltransferase SPB1

Synonyms

2'-O-ribose RNA methyltransferase
S-adenosyl-L-methionine-dependent methyltransferase
Suppressor of PAB1 protein 1

Gene Name

SPB1

Enzyme Class

2.1.1.-

Biological Properties

General Function

Involved in methyltransferase activity

Specific Function

Required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Specifically methylates the guanosine in position 2922 of the 25S rRNA at the stage of 27S pre-rRNA maturation. Methylates also the uridine in position 2921 in the absence of methylation of this residue guided by snoRNA snR52 at the stage of 35S pre-rRNA maturation

Cellular Location

Nucleus, nucleolus

SMPDB Pathways

Not Available

KEGG Pathways

Not Available

SMPDB Reactions

Not Available

KEGG Reactions

Not Available

Metabolites

YMDB ID	Name	View
YMDB00198	S-Adenosylhomocysteine	Show
YMDB00297	S-Adenosylmethionine	Show

GO Classification

Component
organelle
membrane-bounded organelle
intracellular membrane-bounded organelle
nucleus
Function
nucleic acid binding
catalytic activity
transferase activity
transferase activity, transferring one-carbon groups
methyltransferase activity
binding
Process
cellular macromolecule metabolic process
RNA metabolic process
ncRNA metabolic process
one-carbon metabolic process
methylation
metabolic process
rRNA metabolic process
rRNA processing
macromolecule metabolic process
rRNA modification
rRNA methylation
cellular metabolic process

Gene Properties

Chromosome Location

chromosome 3

Locus

YCL054W

Gene Sequence

>2526 bp ATGGGTAAGACACAAAAGAAGAATAGTAAGGGACGTTTAGATAGATACTATTATCTAGCA AAGGAGAAAGGTTATCGTGCTCGTTCATCCTTTAAGATTATTCAGATTAATGAAAAGTAT GGCCACTTCTTAGAAAAATCGAAGGTTGTTATTGATCTGTGTGCTGCTCCTGGTTCATGG TGTCAAGTTGCATCCAAACTCTGTCCTGTCAACTCCTTAATTATTGGTGTTGATATTGTT CCAATGAAGCCGATGCCCAATGTTATAACTTTTCAAAGTGATATTACAACAGAAGATTGT AGATCAAAATTGAGGGGTTATATGAAGACTTGGAAAGCTGATACAGTGTTGCATGATGGT GCTCCTAATGTTGGTTTGGGTTGGGTTCAGGATGCTTTCACCCAATCTCAATTAACCTTA CAAGCTTTGAAGTTGGCCGTCGAAAATTTGGTGGTGAACGGTACTTTTGTTACTAAAATT TTCAGATCCAAGGATTATAATAAATTGATTTGGGTTTTCCAACAATTGTTTGAGAAAGTT GAAGCCACAAAACCACCCGCATCAAGAAATGTTTCTGCAGAAATTTTTGTAGTGTGTAAA GGTTTCAAGGCACCAAAGAGGCTGGACCCAAGATTACTGGATCCAAAAGAAGTTTTTGAA GAATTGCCAGATGGGCAACAGAATATGGAGTCCAAGATTTATAATCCCGAAAAAAAAGTT AGAAAAAGACAAGGTTATGAGGAAGGTGATAATTTACTGTATCATGAAACCTCAATTTTG GATTTTGTCAGGACGGAAGACCCAATAAGCATGCTTGGAGAAATGAATAAGTTTACAATT GATGAGAATGACCATGAATGGAAGATCTTGAAGAAATTGAAACAAACCACAGACGAGTTC CGTTCCTGTATTGAAGATTTAAAAGTCTTGGGTAAAAAGGATTTTAAAATGATCCTAAGA TGGAGAAAGATTGCAAGGGAAATCTTGGGCATCGAAGTTAAGGACGACGCTAAAACAGAA ATTGAAGTAGTACCGTTAACAGAAGAGGAGCAAATTGAAAAAGATTTGCAAGGTTTACAG GAGAAGCAGCGTCTAAATGTCAAGCGCGAAAGAAGAAGGAAGAACGAAATGAAGCAAAAG GAACTACAGAGAATGCAAATGAACATGATAACCCCCACTGATATTGGTATTGAAGCCGCA AGTTTGGGTAAAGAATCGTTGTTTAATTTGAAAACTGCAGAAAAGACTGGTATCTTGAAC GACTTGGCGAAGGGTAAGAAAAGAATGATTTTTACAGACGATGAATTAGCCAAAGATAAT GATATTTACATTGATGAAAACATCATGATCAAAGATAAGGATTCTGCCGCTGATGCGGAC GATTTAGAAAGCGAGTTGAATGCCATGTATAGTGATTATAAAACTAGAAGGTCGGAAAGG GATGCCAAGTTTAGAGCTAAGCAAGCACGTGGTGGCGATAATGAGGAAGAATGGACCGGT TTTAATGAGGGAAGTCTAGAAAAGAAAGAAGAGGAAGGTAAAGATTATATCGAAGACAAT GACGATGAAGGTGTCGAAGGAGACTCCGATGATGATGAAGCCATCACCAATTTGATTAGC AAATTGAAGGGACAAGAAGGTGATCACAAATTGAGTAGTAAGGCACGTATGATTTTCAAT GATCCGATATTTAATAACGTTGAACCTGATTTGCCAGTAAATACCGTCAATGATGGCATA ATGAGTTCCGAGTCTGTTGGTGATATTTCTAAATTAAATAAGAAAAGAAAACATGAAGAA ATGCACCAGAAACAAGATGAAGCAGATTCTTCGGATGAGAGTTCAAGTGATGACTCCGAT TTCGAAATTGTGGCCAACGATAATGCATCAGAAGAATTCGATTCTGATTATGATTCAGAG GAAGAAAAAAATCAAACAAAGAAAGAAAAGCATTCCAGAGACATTGATATTGCTACTGTT GAAGCCATGACTTTGGCACATCAGTTAGCATTGGGTCAGAAAAACAAGCATGATCTTGTT GATGAAGGTTTCAATAGATACACCTTCCGTGACACCGAAAATTTGCCAGATTGGTTTTTA GAAGATGAAAAGGAACATTCAAAGATAAATAAGCCGATTACTAAGGAGGCAGCGATGGCA ATTAAAGAAAAAATAAAGGCAATGAACGCCCGTCCTATTAAGAAGGTTGCTGAAGCTAAG GCAAGAAAGAGGATGCGTGCCGTAGCTCGGTTAGAAAAGATCAAGAAGAAGGCGGGCTTG ATTAACGATGATTCAGACAAGACAGAGAAGGACAAGGCTGAAGAAATTTCTAGATTGATG CGTAAGGTTACCAAAAAACCAAAGACCAAGCCAAAGGTTACTTTGGTTGTTGCCTCAGGT AGGAACAAAGGTTTGGCAGGTAGACCAAAGGGCGTTAAGGGTAAGTATAAGATGGTTGAT GGTGTCATGAAGAATGAACAAAGAGCCCTAAGACGTATCGCAAAGAAGCATCACAAGAAA AAGTAG

Protein Properties

Pfam Domain Function

FtsJ (PF01728 )
Spb1_C (PF07780 )

Protein Residues

841

Protein Molecular Weight

96484.0

Protein Theoretical pI

8.29

Signalling Regions

None

Transmembrane Regions

None

Protein Sequence

>AdoMet-dependent rRNA methyltransferase SPB1 MGKTQKKNSKGRLDRYYYLAKEKGYRARSSFKIIQINEKYGHFLEKSKVVIDLCAAPGSW CQVASKLCPVNSLIIGVDIVPMKPMPNVITFQSDITTEDCRSKLRGYMKTWKADTVLHDG APNVGLGWVQDAFTQSQLTLQALKLAVENLVVNGTFVTKIFRSKDYNKLIWVFQQLFEKV EATKPPASRNVSAEIFVVCKGFKAPKRLDPRLLDPKEVFEELPDGQQNMESKIYNPEKKV RKRQGYEEGDNLLYHETSILDFVRTEDPISMLGEMNKFTIDENDHEWKILKKLKQTTDEF RSCIEDLKVLGKKDFKMILRWRKIAREILGIEVKDDAKTEIEVVPLTEEEQIEKDLQGLQ EKQRLNVKRERRRKNEMKQKELQRMQMNMITPTDIGIEAASLGKESLFNLKTAEKTGILN DLAKGKKRMIFTDDELAKDNDIYIDENIMIKDKDSAADADDLESELNAMYSDYKTRRSER DAKFRAKQARGGDNEEEWTGFNEGSLEKKEEEGKDYIEDNDDEGVEGDSDDDEAITNLIS KLKGQEGDHKLSSKARMIFNDPIFNNVEPDLPVNTVNDGIMSSESVGDISKLNKKRKHEE MHQKQDEADSSDESSSDDSDFEIVANDNASEEFDSDYDSEEEKNQTKKEKHSRDIDIATV EAMTLAHQLALGQKNKHDLVDEGFNRYTFRDTENLPDWFLEDEKEHSKINKPITKEAAMA IKEKIKAMNARPIKKVAEAKARKRMRAVARLEKIKKKAGLINDDSDKTEKDKAEEISRLM RKVTKKPKTKPKVTLVVASGRNKGLAGRPKGVKGKYKMVDGVMKNEQRALRRIAKKHHKK K

References

External Links

Resource	Link
Saccharomyces Genome Database	SPB1
Uniprot ID	P25582
Uniprot Name	SPB1_YEAST
GenBank Gene ID	X59720
Genebank Protein ID	1907120

General Reference

Oliver, S. G., van der Aart, Q. J., Agostoni-Carbone, M. L., Aigle, M., Alberghina, L., Alexandraki, D., Antoine, G., Anwar, R., Ballesta, J. P., Benit, P., et, a. l. .. (1992). "The complete DNA sequence of yeast chromosome III." Nature 357:38-46.1574125
Defoor, E., Debrabandere, R., Keyers, B., Voet, M., Volckaert, G. (1992). "Nucleotide sequence of D10B, a BamHI fragment on the small-ring chromosome III of Saccharomyces cerevisiae." Yeast 8:681-687.1441748
Kressler, D., Rojo, M., Linder, P., Cruz, J. (1999). "Spb1p is a putative methyltransferase required for 60S ribosomal subunit biogenesis in Saccharomyces cerevisiae." Nucleic Acids Res 27:4598-4608.10556316
Pintard, L., Kressler, D., Lapeyre, B. (2000). "Spb1p is a yeast nucleolar protein associated with Nop1p and Nop58p that is able to bind S-adenosyl-L-methionine in vitro." Mol Cell Biol 20:1370-1381.10648622
Nissan, T. A., Bassler, J., Petfalski, E., Tollervey, D., Hurt, E. (2002). "60S pre-ribosome formation viewed from assembly in the nucleolus until export to the cytoplasm." EMBO J 21:5539-5547.12374754
Bonnerot, C., Pintard, L., Lutfalla, G. (2003). "Functional redundancy of Spb1p and a snR52-dependent mechanism for the 2'-O-ribose methylation of a conserved rRNA position in yeast." Mol Cell 12:1309-1315.14636587
Lapeyre, B., Purushothaman, S. K. (2004). "Spb1p-directed formation of Gm2922 in the ribosome catalytic center occurs at a late processing stage." Mol Cell 16:663-669.15546625
Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956