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Glucan 1,3-beta-glucosidase I/II (P23776)

Identification
NameGlucan 1,3-beta-glucosidase I/II
Synonyms
  • Exo-1,3-beta-glucanase I/II
  • Soluble cell wall protein 6
Gene NameEXG1
Enzyme Class
Biological Properties
General FunctionInvolved in hydrolase activity, hydrolyzing O-glycosyl compounds
Specific FunctionGlucanases possibly play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation. This enzyme hydrolyzes both 1,3-beta- and 1,6- beta-linkages and even has beta-glucosidase activity. It could also function biosynthetically as a transglycosylase
Cellular LocationSecreted, cell wall. Secreted
SMPDB Pathways
Starch and sucrose metabolismPW002481 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Starch and sucrose metabolismec00500 Map00500
SMPDB Reactions
Alpha-D-Glucose + 1,3-beta-D-Glucan ↔ 1,3-beta-D-Glucan + water
KEGG Reactions
water + (1->3)-beta-D-glucan D-Glucose
Metabolites
YMDB IDNameView
YMDB00273Alpha-D-GlucoseShow
YMDB00286D-GlucoseShow
YMDB00890waterShow
YMDB163141,3-beta-D-GlucanShow
GO Classification
Component
Not Available
Function
hydrolase activity, hydrolyzing O-glycosyl compounds
catalytic activity
binding
ion binding
cation binding
hydrolase activity
hydrolase activity, acting on glycosyl bonds
Process
metabolic process
primary metabolic process
carbohydrate metabolic process
Gene Properties
Chromosome Locationchromosome 12
LocusYLR300W
Gene Sequence>1347 bp ATGCTTTCGCTTAAAACGTTACTGTGTACGTTGTTGACTGTGTCATCAGTACTCGCTACC CCAGTCCCTGCAAGAGACCCTTCTTCCATTCAATTTGTTCATGAGGAGAACAAGAAAAGA TACTACGATTATGACCACGGTTCCCTCGGAGAACCAATCCGTGGTGTCAACATTGGTGGT TGGTTACTTCTTGAACCATACATTACTCCATCTTTGTTCGAGGCTTTCCGTACAAATGAT GACAACGACGAAGGAATTCCTGTCGACGAATATCACTTCTGTCAATATTTAGGTAAGGAT TTGGCTAAAAGCCGTTTACAGAGCCATTGGTCTACTTTCTACCAAGAACAAGATTTCGCT AATATTGCTTCCCAAGGTTTCAACCTTGTCAGAATTCCTATCGGTTACTGGGCTTTCCAA ACTTTGGACGATGATCCTTATGTTAGCGGCCTACAGGAATCTTACCTAGACCAAGCCATC GGTTGGGCTAGAAACAACAGCTTGAAAGTTTGGGTTGATTTGCATGGTGCCGCTGGTTCG CAGAACGGGTTTGATAACTCTGGTTTGAGAGATTCATACAAGTTTTTGGAAGACAGCAAT TTGGCCGTTACTACAAATGTCTTGAACTACATATTGAAAAAATACTCTGCGGAGGAATAC TTGGACACTGTTATTGGTATCGAATTGATTAATGAGCCATTGGGTCCTGTTCTAGACATG GATAAAATGAAGAATGACTACTTGGCACCTGCTTACGAATACTTGAGAAACAACATCAAG AGTGACCAAGTTATCATCATCCATGACGCTTTCCAACCATACAATTATTGGGATGACTTC ATGACTGAAAACGATGGCTACTGGGGTGTCACTATCGACCATCATCACTACCAAGTCTTT GCTTCTGATCAATTGGAAAGATCCATTGATGAACATATTAAAGTAGCTTGTGAATGGGGT ACCGGAGTTTTGAATGAATCCCACTGGACTGTTTGTGGTGAGTTTGCTGCCGCTTTGACT GATTGTACAAAATGGTTGAATAGTGTTGGCTTCGGCGCTAGATACGACGGTTCTTGGGTC AATGGTGACCAAACATCTTCTTACATTGGCTCTTGTGCTAACAACGATGATATAGCTTAC TGGTCTGACGAAAGAAAGGAAAACACAAGACGTTATGTGGAGGCACAACTAGATGCCTTT GAAATGAGAGGGGGTTGGATTATCTGGTGTTACAAGACAGAATCTAGTTTGGAATGGGAT GCTCAAAGATTGATGTTCAATGGTTTATTCCCTCAACCATTGACTGACAGAAAGTATCCA AACCAATGTGGCACAATTTCTAACTAA
Protein Properties
Pfam Domain Function
Protein Residues448
Protein Molecular Weight51310.5
Protein Theoretical pI4.34
PDB Fileshow
Signalling Regions
  • 1-19
Transmembrane Regions
  • None
Protein Sequence>Glucan 1,3-beta-glucosidase I/II MLSLKTLLCTLLTVSSVLATPVPARDPSSIQFVHEENKKRYYDYDHGSLGEPIRGVNIGG WLLLEPYITPSLFEAFRTNDDNDEGIPVDEYHFCQYLGKDLAKSRLQSHWSTFYQEQDFA NIASQGFNLVRIPIGYWAFQTLDDDPYVSGLQESYLDQAIGWARNNSLKVWVDLHGAAGS QNGFDNSGLRDSYKFLEDSNLAVTTNVLNYILKKYSAEEYLDTVIGIELINEPLGPVLDM DKMKNDYLAPAYEYLRNNIKSDQVIIIHDAFQPYNYWDDFMTENDGYWGVTIDHHHYQVF ASDQLERSIDEHIKVACEWGTGVLNESHWTVCGEFAAALTDCTKWLNSVGFGARYDGSWV NGDQTSSYIGSCANNDDIAYWSDERKENTRRYVEAQLDAFEMRGGWIIWCYKTESSLEWD AQRLMFNGLFPQPLTDRKYPNQCGTISN
References
External Links
ResourceLink
Saccharomyces Genome Database EXG1
Uniprot IDP23776
Uniprot NameEXG1_YEAST
GenBank Gene IDAY693069
Genebank Protein ID51013589
PDB ID
1H4P
General Reference
  • Vazquez de Aldana, C. R., Correa, J., San Segundo, P., Bueno, A., Nebreda, A. R., Mendez, E., del Rey, F. (1991). "Nucleotide sequence of the exo-1,3-beta-glucanase-encoding gene, EXG1, of the yeast Saccharomyces cerevisiae." Gene 97:173-182.1900250
  • Johnston, M., Hillier, L., Riles, L., Albermann, K., Andre, B., Ansorge, W., Benes, V., Bruckner, M., Delius, H., Dubois, E., Dusterhoft, A., Entian, K. D., Floeth, M., Goffeau, A., Hebling, U., Heumann, K., Heuss-Neitzel, D., Hilbert, H., Hilger, F., Kleine, K., Kotter, P., Louis, E. J., Messenguy, F., Mewes, H. W., Hoheisel, J. D., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Nature 387:87-90.9169871
  • Hu, Y., Rolfs, A., Bhullar, B., Murthy, T. V., Zhu, C., Berger, M. F., Camargo, A. A., Kelley, F., McCarron, S., Jepson, D., Richardson, A., Raphael, J., Moreira, D., Taycher, E., Zuo, D., Mohr, S., Kane, M. F., Williamson, J., Simpson, A., Bulyk, M. L., Harlow, E., Marsischky, G., Kolodner, R. D., LaBaer, J. (2007). "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Genome Res 17:536-543.17322287
  • Ramirez, M., Munoz, M. D., Basco, R. D., Gimenez-Gallego, G., Hernandez, L. M., Larriba, G. (1990). "Two glycosylation patterns for a single protein (exoglucanase) in Saccharomyces cerevisiae." FEMS Microbiol Lett 59:43-48.2125957
  • Basco, R. D., Munoz, M. D., Hernandez, L. M., Vazquez de Aldana, C., Larriba, G. (1993). "Reduced efficiency in the glycosylation of the first sequon of Saccharomyces cerevisiae exoglucanase leads to the synthesis and secretion of a new glycoform of the molecule." Yeast 9:221-234.8488724
  • Cappellaro, C., Mrsa, V., Tanner, W. (1998). "New potential cell wall glucanases of Saccharomyces cerevisiae and their involvement in mating." J Bacteriol 180:5030-5037.9748433
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Taylor, S. C., Ferguson, A. D., Bergeron, J. J., Thomas, D. Y. (2004). "The ER protein folding sensor UDP-glucose glycoprotein-glucosyltransferase modifies substrates distant to local changes in glycoprotein conformation." Nat Struct Mol Biol 11:128-134.14730348