tRNA ligase (P09880)

Identification
NametRNA ligase
SynonymsNot Available
Gene NameTRL1
Enzyme Class
Biological Properties
General FunctionInvolved in RNA ligase (ATP) activity
Specific FunctionOne of the two proteins required for the splicing of precursor tRNA molecules containing introns. The ligation activity require three enzymatic activities:phosphorylation of the 5' terminus of the 3' half-tRNA in the presence of ATP, opening of the 2'3'-cyclic phosphodiester bond of the 5' half-tRNA leaving a 2'-phosphomonoester and ligation of the two tRNA halves in an ATP- dependent reaction
Cellular LocationNot Available
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00097Adenosine monophosphateShow
GO Classification
Component
Not Available
Function
ligase activity, forming phosphoric ester bonds
RNA ligase (ATP) activity
RNA ligase activity
catalytic activity
ligase activity
binding
nucleoside binding
purine nucleoside binding
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
Process
RNA processing
RNA splicing
metabolic process
RNA splicing, via endonucleolytic cleavage and ligation
macromolecule metabolic process
tRNA splicing, via endonucleolytic cleavage and ligation
cellular macromolecule metabolic process
RNA metabolic process
Gene Properties
Chromosome Locationchromosome 10
LocusYJL087C
Gene Sequence>2484 bp ATGCCTAGCCCATATGACGGTAAAAGAACAGTAACTCAGCTTGTTAACGAGTTAGAAAAG GCAGAAAAACTATCCGGTAGAGGCAGAGCTTATAGAAGGGTTTGTGACTTATCACACAGC AATAAGAAAGTAATTTCATGGAAATTTAACGAATGGGATTATGGCAAAAATACTATCACT TTGCCATGTAATGCAAGAGGCTTATTTATTAGCGATGATACAACGAATCCAGTTATTGTT GCTAGAGGATACGATAAGTTTTTCAATGTAGGCGAGGTCAACTTTACCAAGTGGAATTGG ATTGAAGAGAACTGTACAGGGCCTTACGATGTCACTATAAAAGCCAATGGTTGTATCATT TTTATATCTGGTTTAGAAGATGGTACCTTGGTAGTTTGTTCGAAGCATTCTACTGGACCC AGAGCAGACGTAGACAGGAACCATGCAGAAGCAGGTGAGAAGCAACTTTTAAGACAACTG GCGGCAATGAACATCAATCGAAGTGATTTTGCTAGAATGCTATATACCCATAATGTCACC GCTGTGGCAGAATATTGCGATGATTCATTTGAAGAACACATCTTAGAGTATCCCCTTGAA AAAGCTGGCCTATACTTACATGGTGTAAATGTTAATAAAGCGGAATTTGAAACTTGGGAT ATGAAAGATGTTTCGCAAATGGCGAGTAAATACGGATTCAGGTGCGTCCAATGCATTACA TCGAACACCTTGGAGGATTTGAAAAAGTTCCTAGATAACTGCTCTGCAACCGGATCTTTT GAAGGGCAAGAGATTGAAGGTTTTGTTATCAGGTGCCACTTGAAGAGTACTGAAAAGCCA TTTTTTTTCAAGTATAAATTTGAAGAACCGTATTTGATGTACCGTCAGTGGAGAGAAGTT ACTAAAGACTATATTTCCAACAAGTCAAGAGTGTTTAAATTTAGAAAGCATAAATTTATA ACCAACAAGTATCTTGATTTCGCAATCCCAATATTGGAGTCATCACCCAAGATCTGTGAG AATTACTTGAAAGGTTTTGGTGTGATTGAATTGAGAAATAAGTTTCTACAATCATATGGC ATGAGTGGGCTGGAAATCTTAAACCACGAAAAAGTGGCCGAGTTGGAATTAAAAAACGCC ATTGATTATGATAAAGTAGACGAACGCACTAAATTTTTAATCTTTCCAATATCAGTTATT GGATGTGGCAAAACAACAACTTCCCAGACATTAGTGAATTTGTTCCCTGACAGCTGGGGT CATATTCAAAATGATGATATTACAGGTAAAGATAAATCTCAATTAATGAAAAAATCATTG GAACTATTATCCAAAAAAGAAATCAAATGTGTCATAGTTGACAGAAACAATCACCAATTC CGCGAAAGAAAGCAATTATTTGAATGGTTGAATGAACTAAAAGAAGATTATTTGGTGTAT GATACGAACATCAAGGTAATCGGCGTGTCATTCGCGCCATATGACAAACTATCGGAAATA AGAGACATAACACTGCAAAGAGTAATCAAAAGAGGTAACAATCACCAAAGTATTAAATGG GACGAATTAGGAGAGAAGAAAGTGGTAGGCATCATGAATGGGTTTTTGAAAAGATATCAA CCTGTTAATTTAGACAAATCACCAGATAATATGTTTGATTTAATGATAGAGTTAGATTTT GGACAAGCAGACTCTTCATTAACCAATGCGAAACAAATCCTCAATGAAATTCATAAAGCT TATCCAATTTTGGTACCAGAGATCCCGAAAGATGATGAAATTGAGACAGCGTTCAGAAGG AGCTTAGATTACAAACCTACTGTGAGAAAAATAGTTGGTAAAGGCAACAATAATCAACAA AAAACGCCTAAGTTAATTAAACCTACATATATCTCAGCTAAAATAGAAAACTACGATGAA ATTATTGAATTGGTCAAGAGGTGTATTGCTAGCGATGCAGAGCTGACTGAGAAATTCAAG CATTTACTAGCTAGTGGAAAAGTTCAGAAGGAACTTCATATAACATTAGGTCATGTCATG TCGTCCCGTGAAAAAGAGGCAAAAAAGTTGTGGAAATCATATTGTAACAGATACACTGAC CAGATAACGGAGTACAATAACAACCGCATAGAAAATGCACAAGGCTCTGGTAATAACCAA AATACGCAGGTAAAAACGACCGACAAACTGAATTTTAGGCTCGAAAAACTATGTTGGGAT GAAAAAATCATTGCTATTGTAGTGGAACTGTCTAAAGATAAAGATGGATGCATAATTGAT GAAAACAATGAGAAAATTAAAGGGTTGTGTTGTCAAAACAAAATTCCTCATATTACGCTG TGCAAACTTGAAAGTGGTGTTAAGGCTGTTTATTCCAATGTTCTATGTGAGAAGGTTGAA TCTGCTGAAGTTGATGAAAATATAAAAGTGGTGAAATTGGACAACTCGAAGGAATTTGTT GGCAGTGTATATTTAAATTTTTAG
Protein Properties
Pfam Domain Function
Protein Residues827
Protein Molecular Weight95336.29688
Protein Theoretical pI7.9
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>tRNA ligase MPSPYDGKRTVTQLVNELEKAEKLSGRGRAYRRVCDLSHSNKKVISWKFNEWDYGKNTIT LPCNARGLFISDDTTNPVIVARGYDKFFNVGEVNFTKWNWIEENCTGPYDVTIKANGCII FISGLEDGTLVVCSKHSTGPRADVDRNHAEAGEKQLLRQLAAMNINRSDFARMLYTHNVT AVAEYCDDSFEEHILEYPLEKAGLYLHGVNVNKAEFETWDMKDVSQMASKYGFRCVQCIT SNTLEDLKKFLDNCSATGSFEGQEIEGFVIRCHLKSTEKPFFFKYKFEEPYLMYRQWREV TKDYISNKSRVFKFRKHKFITNKYLDFAIPILESSPKICENYLKGFGVIELRNKFLQSYG MSGLEILNHEKVAELELKNAIDYDKVDERTKFLIFPISVIGCGKTTTSQTLVNLFPDSWG HIQNDDITGKDKSQLMKKSLELLSKKEIKCVIVDRNNHQFRERKQLFEWLNELKEDYLVY DTNIKVIGVSFAPYDKLSEIRDITLQRVIKRGNNHQSIKWDELGEKKVVGIMNGFLKRYQ PVNLDKSPDNMFDLMIELDFGQADSSLTNAKQILNEIHKAYPILVPEIPKDDEIETAFRR SLDYKPTVRKIVGKGNNNQQKTPKLIKPTYISAKIENYDEIIELVKRCIASDAELTEKFK HLLASGKVQKELHITLGHVMSSREKEAKKLWKSYCNRYTDQITEYNNNRIENAQGSGNNQ NTQVKTTDKLNFRLEKLCWDEKIIAIVVELSKDKDGCIIDENNEKIKGLCCQNKIPHITL CKLESGVKAVYSNVLCEKVESAEVDENIKVVKLDNSKEFVGSVYLNF
References
External Links
ResourceLink
Saccharomyces Genome Database TRL1
Uniprot IDP09880
Uniprot NameTRNL_YEAST
GenBank Gene IDJ03546
Genebank Protein ID171844
General Reference
  • Westaway, S. K., Phizicky, E. M., Abelson, J. (1988). "Structure and function of the yeast tRNA ligase gene." J Biol Chem 263:3171-3176.3277966
  • Miosga, T., Schaaff-Gerstenschlager, I., Chalwatzis, N., Baur, A., Boles, E., Fournier, C., Schmitt, S., Velten, C., Wilhelm, N., Zimmermann, F. K. (1995). "Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(II)2-Cys6 binuclear cluster domain and a putative alpha 2-SCB-alpha 2 binding site." Yeast 11:681-689.7483841
  • Galibert, F., Alexandraki, D., Baur, A., Boles, E., Chalwatzis, N., Chuat, J. C., Coster, F., Cziepluch, C., De Haan, M., Domdey, H., Durand, P., Entian, K. D., Gatius, M., Goffeau, A., Grivell, L. A., Hennemann, A., Herbert, C. J., Heumann, K., Hilger, F., Hollenberg, C. P., Huang, M. E., Jacq, C., Jauniaux, J. C., Katsoulou, C., Karpfinger-Hartl, L., et, a. l. .. (1996). "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." EMBO J 15:2031-2049.8641269
  • Xu, Q., Teplow, D., Lee, T. D., Abelson, J. (1990). "Domain structure in yeast tRNA ligase." Biochemistry 29:6132-6138.2207062
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106