Probable kynurenine formamidase (Q04066)

Identification
NameProbable kynurenine formamidase
Synonyms
  • Biosynthesis of nicotinic acid protein 7
  • Probable N-formylkynurenine formamidase
Gene NameBNA7
Enzyme Class
Biological Properties
General FunctionInvolved in hydrolase activity
Specific FunctionCatalyzes the hydrolysis of N-formyl-L-kynurenine to L- kynurenine, the second step in the conversion of tryptophan to nicotinic acid, NAD(H) and NADP(H)
Cellular LocationCytoplasmic
SMPDB Pathways
NAD metabolismPW002421 ThumbThumb?image type=greyscaleThumb?image type=simple
Tryptophan metabolismPW002442 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Glyoxylate and dicarboxylate metabolismec00630 Map00630
Tryptophan metabolismec00380 Map00380
SMPDB Reactions
N'-Formylkynurenine + water → Formic acid + hydron + L-Kynurenine
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00234N'-FormylkynurenineShow
YMDB002782-Aminobenzoic acidShow
YMDB00375L-KynurenineShow
YMDB00385Formic acidShow
YMDB00725N-Formyl-L-kynurenineShow
YMDB00811N-Formylanthranilic acidShow
YMDB00862hydronShow
YMDB00890waterShow
GO Classification
Component
Not Available
Function
hydrolase activity
catalytic activity
Process
metabolic process
Gene Properties
Chromosome Locationchromosome 4
LocusYDR428C
Gene Sequence>786 bp ATGTCAAATACCGTAAGAGCCATTTCACCAGATATAACACTATTCAACAAGACATTGACA TTCCAAGAAATCTCACAAAACACTAGAGAGGCTGTCATATACATTCATGGAGGTGCATGG AACGATCCTGAGAATACGCCCAATGATTTTAATCAATTAGCCAATACCATCAAATCAATG GATACTGAGTCAACGGTTTGTCAATACTCTATAGAATACAGGTTATCACCAGAGATCACA AATCCAAGAAACTTATATGATGCTGTGTCAAATATAACAAGGCTTGTTAAAGAAAAGGGA TTAACGAACATTAATATGGTGGGACATTCCGTTGGAGCTACCTTTATTTGGCAGATATTA GCTGCACTCAAAGATCCTCAAGAAAAAATGAGTGAAGCGCAATTGCAAATGTTGGGACTT TTGCAGATCGTGAAGAGAGTATTTCTATTGGATGGTATATACTCGTTAAAGGAACTGTTG GTAGAATACCCAGAGTATGATTGTTTTACAAGACTTGCCTTCCCTGATGGTATTCAAATG TACGAGGAAGAGCCCTCGAGAGTAATGCCTTACGTGAAGAAAGCCCTTTCTCGCTTCAGT ATTGATATGCACCTAGTGCATTCTTATAGCGACGAGTTATTAACGTTAAGACAAACTAAT TGTTTAATTTCTTGTCTTCAGGATTATCAGTTAAGTTTCAAGTTGTATTTGGACGATTTG GGTTTGCATAATGATGTTTATAAGAATGGTAAAGTTGCGAAGTATATTTTTGACAATATT TGTTGA
Protein Properties
Pfam Domain Function
Protein Residues261
Protein Molecular Weight29991.0
Protein Theoretical pI4.98
PDB Fileshow
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Probable kynurenine formamidase MSNTVRAISPDITLFNKTLTFQEISQNTREAVIYIHGGAWNDPENTPNDFNQLANTIKSM DTESTVCQYSIEYRLSPEITNPRNLYDAVSNITRLVKEKGLTNINMVGHSVGATFIWQIL AALKDPQEKMSEAQLQMLGLLQIVKRVFLLDGIYSLKELLVEYPEYDCFTRLAFPDGIQM YEEEPSRVMPYVKKALSRFSIDMHLVHSYSDELLTLRQTNCLISCLQDYQLSFKLYLDDL GLHNDVYKNGKVAKYIFDNIC
References
External Links
ResourceLink
Saccharomyces Genome Database BNA7
Uniprot IDQ04066
Uniprot NameBNA7_YEAST
GenBank Gene IDU33007
Genebank Protein ID927719
PDB ID
1VKH
General Reference
  • Jacq, C., Alt-Morbe, J., Andre, B., Arnold, W., Bahr, A., Ballesta, J. P., Bargues, M., Baron, L., Becker, A., Biteau, N., Blocker, H., Blugeon, C., Boskovic, J., Brandt, P., Bruckner, M., Buitrago, M. J., Coster, F., Delaveau, T., del Rey, F., Dujon, B., Eide, L. G., Garcia-Cantalejo, J. M., Goffeau, A., Gomez-Peris, A., Zaccaria, P., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Nature 387:75-78.9169867
  • Baxter, S. M., Rosenblum, J. S., Knutson, S., Nelson, M. R., Montimurro, J. S., Di Gennaro, J. A., Speir, J. A., Burbaum, J. J., Fetrow, J. S. (2004). "Synergistic computational and experimental proteomics approaches for more accurate detection of active serine hydrolases in yeast." Mol Cell Proteomics 3:209-225.14645503
  • Gruhler, A., Olsen, J. V., Mohammed, S., Mortensen, P., Faergeman, N. J., Mann, M., Jensen, O. N. (2005). "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Mol Cell Proteomics 4:310-327.15665377
  • Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
  • Wogulis, M., Chew, E. R., Donohoue, P. D., Wilson, D. K. (2008). "Identification of formyl kynurenine formamidase and kynurenine aminotransferase from Saccharomyces cerevisiae using crystallographic, bioinformatic and biochemical evidence." Biochemistry 47:1608-1621.18205391
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956
  • Arndt, J. W., Schwarzenbacher, R., Page, R., Abdubek, P., Ambing, E., Biorac, T., Canaves, J. M., Chiu, H. J., Dai, X., Deacon, A. M., DiDonato, M., Elsliger, M. A., Godzik, A., Grittini, C., Grzechnik, S. K., Hale, J., Hampton, E., Han, G. W., Haugen, J., Hornsby, M., Klock, H. E., Koesema, E., Kreusch, A., Kuhn, P., Jaroszewski, L., Lesley, S. A., Levin, I., McMullan, D., McPhillips, T. M., Miller, M. D., Morse, A., Moy, K., Nigoghossian, E., Ouyang, J., Peti, W. S., Quijano, K., Reyes, R., Sims, E., Spraggon, G., Stevens, R. C., van den Bedem, H., Velasquez, J., Vincent, J., von Delft, F., Wang, X., West, B., White, A., Wolf, G., Xu, Q., Zagnitko, O., Hodgson, K. O., Wooley, J., Wilson, I. A. (2005). "Crystal structure of an alpha/beta serine hydrolase (YDR428C) from Saccharomyces cerevisiae at 1.85 A resolution." Proteins 58:755-758.15624212