NAD-dependent deacetylase HST1 (P53685)

Identification
NameNAD-dependent deacetylase HST1
Synonyms
  • Homologous to SIR2 protein 1
Gene NameHST1
Enzyme ClassNot Available
Biological Properties
General FunctionTranscription
Specific FunctionNAD-dependent histone deacetylase involved in telomeric silencing. Histone deacetylase proteins act via the formation of large multiprotein complexes that are responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Restores silencing at HMR in SIR2 mutants when overexpressed. Required to repress middle sporulation genes during vegetative growth. Acts as a sensor of NAD(+) levels and regulator of NAD(+) biosynthesis. Regulates the gene expression of de novo NAD(+) biosynthesis genes
Cellular LocationNucleus.
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG Reactions
NAD + water ↔ hydron + Niacinamide + Adenosine diphosphate ribose
Metabolites
YMDB IDNameView
GO Classification
Function
ion binding
cation binding
metal ion binding
transition metal ion binding
zinc ion binding
catalytic activity
NAD binding
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
histone deacetylase activity
nucleotide binding
NAD-dependent histone deacetylase activity
NAD or NADH binding
hydrolase activity
binding
Process
regulation of macromolecule metabolic process
regulation of gene expression
regulation of transcription
cellular process
gene silencing
chromatin silencing
macromolecule modification
macromolecule metabolic process
protein modification process
post-translational protein modification
protein amino acid deacetylation
regulation of transcription, DNA-dependent
biological regulation
metabolic process
regulation of biological process
regulation of metabolic process
Gene Properties
Chromosome LocationNot Available
Locus
Gene Sequence>1512 bp ATGAACATATTGCTAATGCAACGGATAGTTAGTTTTATTTTGGTGGTTAGTCAAGGGCGA TACTTTCATGTTGGAGAGCTGACCATGACAATGTTGAAAAGACCTCAAGAAGAAGAGTCA GATAATAATGCTACGAAGAAGCTGAAAACGAGATTAACGTACCCTTGTATCCTCGGGAAG GATAAGGTGACGGGCAAATTCATCTTTCCTGCAATCACTAAAGATGATGTTATGAATGCA AGACTGTTTTTGAAGGACAACGATTTGAAAACTTTTTTAGAATATTTTTTGCCAGTGGAA GTCAACTCGATCTACATTTATTTCATGATAAAATTGCTGGGATTCGACGTCAAAGATAAA GAACTATTTATGGCGTTAAACTCTAATATCACTTCTAATAAAGAACGTTCTAGTGCGGAA CTATCGTCTATACATGCAAAAGCTGAAGATGAGGACGAGTTGACCGATCCCTTAGAGAAA AAGCATGCAGTAAAATTAATCAAAGATTTACAAAAAGCCATAAATAAAGTCCTTAGTACA AGGCTTCGATTACCCAACTTCAATACGATTGATCATTTCACCGCCACTTTGCGTAACGCC AAAAAAATTTTGGTTCTGACGGGCGCTGGAGTTTCCACTTCTTTAGGTATTCCCGATTTT AGATCATCTGAGGGGTTTTATTCTAAGATCCGACACCTTGGATTGGAAGACCCTCAGGAT GTTTTCAACTTGGATATATTCTTACAGGACCCTTCTGTTTTTTACAATATTGCCCATATG GTTTTACCACCAGAGAACATGTATTCTCCGTTGCACAGCTTCATTAAAATGCTACAAGAC AAGGGCAAACTATTAAGAAACTACACACAGAATATAGATAACTTAGAATCGTATGCAGGG ATAGATCCTGATAAATTAGTGCAATGTCACGGTTCATTCGCCACTGCATCTTGTGTGACT TGCCATTGGCAAATACCTGGTGAGAAAATATTCGAAAATATTAGAAACCTTGAACTACCA TTATGTCCCTACTGTTATCAAAAAAGAAAACAATATTTTCCTATGAGTAATGGCAATAAT ACGGTGCAAACAAACATAAATTTCAACTCACCCATTTTAAAGTCGTATGGTGTTTTGAAA CCAGACATGACATTTTTTGGCGAGGCCCTGCCATCGCGATTTCATAAGACCATCCGCAAA GATATACTGGAATGCGATCTATTGATTTGTATAGGAACAAGTTTGAAAGTGGCTCCTGTG TCTGAAATTGTCAATATGGTACCTTCACATGTTCCGCAGATTTTGATCAATAGGGATATG GTTACACATGCAGAGTTTGACTTAAACCTGTTGGGATTCTGTGATGACGTGGCCAGTCTC GTAGCTAAAAAATGCCATTGGGATATACCGCATAAAAAATGGCAAGATCTAAAGAAGATA GATTACAACTGTACGGAAATAGATAAAGGTACGTATAAGATTAAGAAACAGCCACGAAAG AAACAACAGTAA
Protein Properties
Pfam Domain Function
Protein Residues503
Protein Molecular Weight57701.5
Protein Theoretical pI8.68
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>NAD-dependent deacetylase HST1 MNILLMQRIVSFILVVSQGRYFHVGELTMTMLKRPQEEESDNNATKKLKTRLTYPCILGK DKVTGKFIFPAITKDDVMNARLFLKDNDLKTFLEYFLPVEVNSIYIYFMIKLLGFDVKDK ELFMALNSNITSNKERSSAELSSIHAKAEDEDELTDPLEKKHAVKLIKDLQKAINKVLST RLRLPNFNTIDHFTATLRNAKKILVLTGAGVSTSLGIPDFRSSEGFYSKIRHLGLEDPQD VFNLDIFLQDPSVFYNIAHMVLPPENMYSPLHSFIKMLQDKGKLLRNYTQNIDNLESYAG IDPDKLVQCHGSFATASCVTCHWQIPGEKIFENIRNLELPLCPYCYQKRKQYFPMSNGNN TVQTNINFNSPILKSYGVLKPDMTFFGEALPSRFHKTIRKDILECDLLICIGTSLKVAPV SEIVNMVPSHVPQILINRDMVTHAEFDLNLLGFCDDVASLVAKKCHWDIPHKKWQDLKKI DYNCTEIDKGTYKIKKQPRKKQQ
References
External Links
ResourceLink
Saccharomyces Genome Database HST1
Uniprot IDP53685
Uniprot NameHST1_YEAST
GenBank Gene IDL47120
Genebank Protein ID972893
General Reference
  • Brachmann, C. B., Sherman, J. M., Devine, S. E., Cameron, E. E., Pillus, L., Boeke, J. D. (1995). "The SIR2 gene family, conserved from bacteria to humans, functions in silencing, cell cycle progression, and chromosome stability." Genes Dev 9:2888-2902.7498786
  • Derbyshire, M. K., Weinstock, K. G., Strathern, J. N. (1996). "HST1, a new member of the SIR2 family of genes." Yeast 12:631-640.8810037
  • Tzermia, M., Katsoulou, C., Alexandraki, D. (1997). "Sequence analysis of a 33.2 kb segment from the left arm of yeast chromosome XV reveals eight known genes and ten new open reading frames including homologues of ABC transporters, inositol phosphatases and human expressed sequence tags." Yeast 13:583-589.9178509
  • Dujon, B., Albermann, K., Aldea, M., Alexandraki, D., Ansorge, W., Arino, J., Benes, V., Bohn, C., Bolotin-Fukuhara, M., Bordonne, R., Boyer, J., Camasses, A., Casamayor, A., Casas, C., Cheret, G., Cziepluch, C., Daignan-Fornier, B., Dang, D. V., de Haan, M., Delius, H., Durand, P., Fairhead, C., Feldmann, H., Gaillon, L., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Nature 387:98-102.9169874
  • Xie, J., Pierce, M., Gailus-Durner, V., Wagner, M., Winter, E., Vershon, A. K. (1999). "Sum1 and Hst1 repress middle sporulation-specific gene expression during mitosis in Saccharomyces cerevisiae." EMBO J 18:6448-6454.10562556
  • Bedalov, A., Hirao, M., Posakony, J., Nelson, M., Simon, J. A. (2003). "NAD+-dependent deacetylase Hst1p controls biosynthesis and cellular NAD+ levels in Saccharomyces cerevisiae." Mol Cell Biol 23:7044-7054.12972620
  • Smith, J. S., Brachmann, C. B., Celic, I., Kenna, M. A., Muhammad, S., Starai, V. J., Avalos, J. L., Escalante-Semerena, J. C., Grubmeyer, C., Wolberger, C., Boeke, J. D. (2000). "A phylogenetically conserved NAD+-dependent protein deacetylase activity in the Sir2 protein family." Proc Natl Acad Sci U S A 97:6658-6663.10841563
  • Pijnappel, W. W., Schaft, D., Roguev, A., Shevchenko, A., Tekotte, H., Wilm, M., Rigaut, G., Seraphin, B., Aasland, R., Stewart, A. F. (2001). "The S. cerevisiae SET3 complex includes two histone deacetylases, Hos2 and Hst1, and is a meiotic-specific repressor of the sporulation gene program." Genes Dev 15:2991-3004.11711434
  • McCord, R., Pierce, M., Xie, J., Wonkatal, S., Mickel, C., Vershon, A. K. (2003). "Rfm1, a novel tethering factor required to recruit the Hst1 histone deacetylase for repression of middle sporulation genes." Mol Cell Biol 23:2009-2016.12612074
  • Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106