Vacuolar amino acid transporter 4 (P50944)

Identification
NameVacuolar amino acid transporter 4
SynonymsNot Available
Gene NameAVT4
Enzyme ClassNot Available
Biological Properties
General FunctionAmino acid transport and metabolism
Specific FunctionInvolved in amino acid efflux from the vacuole to the cytoplasm. Capable of transporting large neutral amino acids including tyrosine, glutamine, asparagine, isoleucine and leucine
Cellular LocationVacuole membrane; Multi-pass membrane protein
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00002L-GlutamineShow
YMDB00038L-IsoleucineShow
YMDB00226L-AsparagineShow
YMDB00364L-TyrosineShow
YMDB00387L-LeucineShow
YMDB00862hydronShow
GO ClassificationNot Available
Gene Properties
Chromosome Locationchromosome 14
LocusYNL101W
Gene Sequence>2142 bp ATGGTCACTAACAATGGAGATGGTGAGCACCTTGGGATCCGAAGAAACGGAAATTTAAGG CATCCTTCCAATAATATGAAAATTCCCAGAAGGGCTCAATCAACAGTTCTCAATTCAAAC CCATTTTATAGTAGGAAGTATTCTATGTCTACCTTAACGCCGAGGGATATATGTCGAAGC GTTGATTCTAGAGTATTTGTGGATATGTCTTCTCCCAATTTTCAGACTCTGGAGGATCCC CACAGAGATGAGATTATAAACAGTGTACGGCTTAACTACTTGAACAGTAGCAAAAGAAGT TCCGTCTCTCATGGAAATGAAGCAATCCCTAGGGTTAATCCGACTAAGAATTCTTCAGCA AGTACCATAGCTGCAGCAAATGTTGATAGTGATGACGATGAGACTAACCTGAGTAGCGCT GGAGGCGACATAACGCATGATATTTACAAGTTGGTTAAAGCAGAGGATCCTAAACGGCTC AGACGCCCTCGGTCCATGGAAAATGTAACACCTAAAATTGAGCACCATACTAAATTGTCA TCAGCAAGTGGTTTAAATGTCCCCGGTGGCTTCAGAAGGGAATTTATTGTCAACAAAAAA AGGCAAGAACATCAATTAAATGATTCCGCTAGCTCAGACTTCACTTCGCATGAAAGCGAT TCGATTAACCAGTCATCTCCATCTTCTAACCAAGATATCGATAAGGTCCCCTTTTTAACA AGAAACTTTTTGGAATTTCTATACGTGTTTGGACATTTTGCTGGTGAATCCTTCGAGGAT GATTTTATCCCTGATAGCTCAAACATGATGATACGCGGAGAAGATGAAAGGAGTGCATTG CTGTCTCGGCCTGACCATATGAAAGTATTACCTTCAGCCAAAGGTACGACTTCGACAAAG AAGGTTTTTTTGATATTGCTTAAATCTTTCATTGGAACTGGCGTTTTATTTTTACCTAAT GCCTTTCATAATGGTGGTTTGTTTTTCTCAGTAAGTATGCTGGCCTTTTTTGGAATTTAT TCATACTGGTGTTACTACATATTGGTGCAGGCCAAATCTTCATGCGGTGTATCCTCCTTT GGTGATATTGGTTTAAAACTGTATGGACCTTGGATGAGAATAATAATTCTTTTCTCCCTA GTAATAACTCAAGTTGGATTCTCTGGAGCTTATATGATATTTACTGCAAAAAATTTACAA GCTTTTCTTGATAACGTATTCCATGTGGGCGTCCTACCTTTATCTTACCTGATGGTCTTT CAAACGATCATTTTTATTCCACTTTCGTTTATTAGAAACATTTCGAAATTGTCACTACCA TCTTTACTAGCAAATTTTTTCATCATGGCTGGTTTAGTCATTGTCATTATTTTTACAGCC AAAAGGTTGTTTTTTGACCTGATGGGGACCCCAGCAATGGGTGTCGTCTATGGCTTAAAT GCAGACCGTTGGACGCTGTTTATCGGGACGGCAATATTTGCTTTCGAAGGAATCGGGTTA ATTATTCCTGTTCAAGACTCAATGAGAAATCCTGAAAAATTTCCTTTAGTACTTGCTTTG GTGATTCTAACGGCAACCATACTTTTCATTTCCATAGCTACATTGGGATATTTGGCATAC GGCTCAAATGTTCAAACGGTTATACTGTTGAACTTACCTCAAAGTAACATTTTTGTAAAC TTAATCCAGCTGTTCTATTCCATCGCAATTATGTTGTCCACACCTTTGCAGTTGTTTCCA GCTATTAAAATTATTGAAAATAAGTTTTTTCCAAAGTTCACTAAAATATATGTCAAGCAC GATGATCTAACAACAAGAGTCGAATTACGTCCTAATTCAGGAAAGCTGAACTGGAAAATT AAATGGCTGAAGAACTTTATTCGTTCTATAATCGTAATCATTGTTGTATCAATTGCTTAC TTTGGGTCTGATAATTTAGACAAGTTCGTTTCAGTTATAGGATCCCTCGCCTGTATTCCT TTGGTATATATATACCCATCGATGCTGCATTTACGGGGCAATAGCCTTCCAGAGACTAAA GGGGAATTTTGGAGATTCAAACCAATGTTAGATACCATTTTAATTTTCTTCGGTATTGCA AGCATGCTTTATACGTCTTACCAGAGTATATTCGGTGTCTAG
Protein Properties
Pfam Domain Function
Protein Residues713
Protein Molecular Weight80025.0
Protein Theoretical pI9.73
Signalling Regions
  • None
Transmembrane Regions
  • 243-263
  • 302-322
  • 327-347
  • 374-394
  • 411-431
  • 439-459
  • 484-504
  • 516-536
  • 562-582
  • 622-642
  • 649-669
  • 693-711
Protein Sequence>Vacuolar amino acid transporter 4 MVTNNGDGEHLGIRRNGNLRHPSNNMKIPRRAQSTVLNSNPFYSRKYSMSTLTPRDICRS VDSRVFVDMSSPNFQTLEDPHRDEIINSVRLNYLNSSKRSSVSHGNEAIPRVNPTKNSSA STIAAANVDSDDDETNLSSAGGDITHDIYKLVKAEDPKRLRRPRSMENVTPKIEHHTKLS SASGLNVPGGFRREFIVNKKRQEHQLNDSASSDFTSHESDSINQSSPSSNQDIDKVPFLT RNFLEFLYVFGHFAGESFEDDFIPDSSNMMIRGEDERSALLSRPDHMKVLPSAKGTTSTK KVFLILLKSFIGTGVLFLPNAFHNGGLFFSVSMLAFFGIYSYWCYYILVQAKSSCGVSSF GDIGLKLYGPWMRIIILFSLVITQVGFSGAYMIFTAKNLQAFLDNVFHVGVLPLSYLMVF QTIIFIPLSFIRNISKLSLPSLLANFFIMAGLVIVIIFTAKRLFFDLMGTPAMGVVYGLN ADRWTLFIGTAIFAFEGIGLIIPVQDSMRNPEKFPLVLALVILTATILFISIATLGYLAY GSNVQTVILLNLPQSNIFVNLIQLFYSIAIMLSTPLQLFPAIKIIENKFFPKFTKIYVKH DDLTTRVELRPNSGKLNWKIKWLKNFIRSIIVIIVVSIAYFGSDNLDKFVSVIGSLACIP LVYIYPSMLHLRGNSLPETKGEFWRFKPMLDTILIFFGIASMLYTSYQSIFGV
References
External Links
ResourceLink
Saccharomyces Genome Database AVT4
Uniprot IDP50944
Uniprot NameAVT4_YEAST
GenBank Gene IDZ50161
Genebank Protein ID929852
General Reference
  • Saiz, J. E., Buitrago, M. J., Soler-Mira, A., Del Rey, F., Revuelta, J. L. (1996). "The sequence of a 21.3 kb DNA fragment from the left arm of yeast chromosome XIV reveals LEU4, MET4, POL1, RAS2, and six new open reading frames." Yeast 12:403-409.8701612
  • Philippsen, P., Kleine, K., Pohlmann, R., Dusterhoft, A., Hamberg, K., Hegemann, J. H., Obermaier, B., Urrestarazu, L. A., Aert, R., Albermann, K., Altmann, R., Andre, B., Baladron, V., Ballesta, J. P., Becam, A. M., Beinhauer, J., Boskovic, J., Buitrago, M. J., Bussereau, F., Coster, F., Crouzet, M., D'Angelo, M., Dal Pero, F., De Antoni, A., Del Rey, F., Doignon, F., Domdey, H., Dubois, E., Fiedler, T., Fleig, U., Floeth, M., Fritz, C., Gaillardin, C., Garcia-Cantalejo, J. M., Glansdorff, N. N., Goffeau, A., Gueldener, U., Herbert, C., Heumann, K., Heuss-Neitzel, D., Hilbert, H., Hinni, K., Iraqui Houssaini, I., Jacquet, M., Jimenez, A., Jonniaux, J. L., Karpfinger, L., Lanfranchi, G., Lepingle, A., Levesque, H., Lyck, R., Maftahi, M., Mallet, L., Maurer, K. C., Messenguy, F., Mewes, H. W., Mosti, D., Nasr, F., Nicaud, J. M., Niedenthal, R. K., Pandolfo, D., Pierard, A., Piravandi, E., Planta, R. J., Pohl, T. M., Purnelle, B., Rebischung, C., Remacha, M., Revuelta, J. L., Rinke, M., Saiz, J. E., Sartorello, F., Scherens, B., Sen-Gupta, M., Soler-Mira, A., Urbanus, J. H., Valle, G., Van Dyck, L., Verhasselt, P., Vierendeels, F., Vissers, S., Voet, M., Volckaert, G., Wach, A., Wambutt, R., Wedler, H., Zollner, A., Hani, J. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Nature 387:93-98.9169873
  • Russnak, R., Konczal, D., McIntire, S. L. (2001). "A family of yeast proteins mediating bidirectional vacuolar amino acid transport." J Biol Chem 276:23849-23857.11274162
  • Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258
  • Chi, A., Huttenhower, C., Geer, L. Y., Coon, J. J., Syka, J. E., Bai, D. L., Shabanowitz, J., Burke, D. J., Troyanskaya, O. G., Hunt, D. F. (2007). "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Proc Natl Acad Sci U S A 104:2193-2198.17287358
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956