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Identification
NamePlasma membrane ATPase 1
Synonyms
  • Proton pump 1
Gene NamePMA1
Enzyme Class
Biological Properties
General FunctionInvolved in nucleotide binding
Specific FunctionThe plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H(+)-symport. The resulting external acidification and/or internal alkinization may mediate growth responses
Cellular LocationCell membrane; Multi-pass membrane protein
SMPDB Pathways
Oxidative phosphorylationPW002461 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Oxidative phosphorylationec00190 Map00190
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00109Adenosine triphosphateShow
YMDB00862hydronShow
YMDB00890waterShow
YMDB00907phosphateShow
YMDB00914ADPShow
GO Classification
Component
cell part
membrane
membrane part
intrinsic to membrane
integral to membrane
Function
hydrolase activity
binding
substrate-specific transmembrane transporter activity
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
nucleoside binding
ion transmembrane transporter activity
pyrophosphatase activity
purine nucleoside binding
nucleoside-triphosphatase activity
adenyl nucleotide binding
ATPase activity, coupled to transmembrane movement of ions
ATPase activity
adenyl ribonucleotide binding
ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism
ATP binding
hydrolase activity, acting on acid anhydrides
transporter activity
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
catalytic activity
transmembrane transporter activity
Process
nucleotide metabolic process
establishment of localization
purine nucleoside triphosphate biosynthetic process
purine nucleotide metabolic process
transport
purine ribonucleoside triphosphate biosynthetic process
purine nucleotide biosynthetic process
ATP biosynthetic process
ion transport
nitrogen compound metabolic process
cellular nitrogen compound metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleoside phosphate metabolic process
cation transport
metabolic process
Gene Properties
Chromosome Locationchromosome 7
LocusYGL008C
Gene Sequence>2757 bp ATGACTGATACATCATCCTCTTCATCATCCTCTTCAGCATCTTCTGTTTCAGCTCATCAG CCAACTCAAGAAAAGCCTGCTAAGACTTACGATGACGCTGCATCTGAATCTTCTGACGAT GACGATATCGATGCTTTAATCGAAGAACTACAATCTAATCACGGTGTCGACGACGAAGAC AGTGATAACGATGGTCCAGTTGCCGCCGGTGAAGCTAGACCAGTTCCAGAAGAATATTTA CAAACTGACCCATCTTACGGTTTAACTTCCGATGAAGTTTTGAAAAGAAGAAAGAAGTAC GGTTTGAATCAAATGGCTGACGAAAAAGAATCTTTAGTCGTTAAGTTCGTTATGTTTTTC GTCGGTCCAATTCAATTCGTTATGGAAGCCGCTGCTATTTTGGCTGCCGGTTTGTCCGAT TGGGTCGATTTCGGTGTTATCTGTGGTTTGTTAATGTTAAACGCTGGTGTTGGTTTCGTT CAAGAATTCCAAGCTGGTTCTATTGTCGATGAATTGAAAAAGACTTTGGCTAACACTGCT GTTGTTATCAGAGACGGTCAATTGGTTGAAATTCCAGCCAACGAAGTCGTCCCAGGTGAT ATTTTGCAATTGGAAGATGGTACTGTTATCCCAACTGATGGTCGTATTGTCACTGAAGAC TGTTTCTTGCAAATCGATCAATCTGCTATTACTGGTGAATCTTTGGCTGTCGACAAACAT TACGGTGACCAAACTTTCTCTTCTTCCACTGTTAAGAGAGGTGAAGGTTTCATGGTTGTT ACCGCTACCGGTGACAACACTTTCGTTGGTAGAGCTGCTGCTTTGGTTAACAAAGCCGCT GGTGGTCAAGGTCATTTCACTGAAGTTTTGAACGGTATTGGTATTATCTTATTGGTTTTG GTCATTGCCACTTTGTTGTTGGTCTGGACTGCTTGTTTCTACAGAACCAACGGTATTGTT AGAATCTTGAGATACACTCTAGGTATTACTATTATTGGTGTCCCAGTCGGTTTGCCAGCT GTCGTTACCACCACTATGGCCGTCGGTGCTGCTTACTTGGCTAAGAAACAAGCCATTGTT CAAAAGTTGTCTGCTATTGAATCCTTGGCTGGTGTCGAAATCTTGTGTTCCGACAAAACC GGTACTTTGACCAAGAACAAGTTGTCCTTGCACGAACCATACACTGTTGAAGGTGTTTCT CCAGACGACTTGATGTTGACTGCTTGTTTGGCTGCTTCCAGAAAGAAGAAGGGTTTGGAT GCTATTGATAAGGCTTTCTTGAAGTCTTTGAAGCAATATCCAAAGGCTAAGGACGCTTTG ACCAAGTACAAGGTTTTGGAATTCCATCCATTTGACCCTGTCTCCAAGAAGGTTACTGCC GTTGTCGAATCTCCAGAAGGTGAAAGAATTGTTTGTGTTAAGGGTGCTCCATTATTCGTC TTGAAGACTGTCGAAGAAGATCACCCAATCCCAGAAGATGTCCATGAAAACTACGAAAAC AAGGTTGCTGAATTGGCTTCTAGAGGGTTCCGTGCTTTAGGTGTTGCTAGAAAGAGAGGT GAAGGTCACTGGGAAATCTTGGGTGTTATGCCATGTATGGATCCACCAAGAGACGATACT GCTCAAACTGTTAGCGAAGCTAGACACTTGGGTCTAAGAGTTAAGATGTTAACTGGTGAT GCTGTTGGTATTGCTAAGGAAACTTGTAGACAATTGGGTTTGGGTACCAACATTTACAAC GCTGAAAGATTAGGTCTAGGTGGCGGTGGTGACATGCCAGGTTCTGAATTAGCTGATTTT GTTGAAAATGCCGATGGTTTCGCTGAAGTTTTCCCACAACATAAATACAGAGTTGTTGAA ATCTTGCAAAACAGAGGTTACTTGGTTGCTATGACTGGTGATGGTGTTAACGATGCTCCA TCTTTGAAGAAGGCTGATACTGGTATTGCTGTCGAAGGTGCTACTGATGCTGCCAGATCT GCTGCTGATATTGTTTTCTTGGCTCCTGGTCTATCTGCTATTATTGATGCTTTGAAGACC TCCAGACAAATTTTTCACAGAATGTACTCTTACGTTGTTTACCGTATTGCTTTGTCTCTA CATTTGGAAATCTTCTTGGGTCTATGGATTGCTATTTTGGATAACTCTTTGGACATTGAT TTGATTGTTTTCATCGCTATTTTCGCTGATGTTGCTACTTTGGCTATTGCTTACGATAAT GCTCCTTACTCTCCAAAGCCCGTTAAATGGAACCTACCAAGATTATGGGGTATGTCTATT ATTTTGGGCATAGTTTTAGCTATAGGTTCTTGGATTACCTTGACTACTATGTTCTTACCA AAGGGTGGTATTATCCAAAACTTCGGTGCTATGAACGGTATTATGTTCTTGCAAATTTCC TTGACTGAAAACTGGTTGATTTTCATTACCAGAGCTGCTGGTCCATTCTGGTCTTCTATC CCATCCTGGCAATTGGCTGGTGCCGTCTTCGCTGTCGACATCATCGCTACCATGTTTACC TTATTCGGTTGGTGGTCTGAAAACTGGACTGATATTGTTACTGTCGTCCGTGTCTGGATC TGGTCTATCGGTATCTTCTGTGTTTTGGGTGGTTTCTACTACGAAATGTCCACTTCTGAA GCCTTTGACAGATTGATGAACGGTAAGCCAATGAAGGAAAAGAAGTCTACCAGAAGTGTC GAAGACTTCATGGCTGCTATGCAAAGAGTCTCTACTCAACACGAAAAGGAAACCTAA
Protein Properties
Pfam Domain Function
Protein Residues918
Protein Molecular Weight99618.39844
Protein Theoretical pI4.72
Signalling Regions
  • None
Transmembrane Regions
  • 116-136
  • 141-160
  • 292-313
  • 326-347
  • 720-738
  • 755-774
  • 825-845
  • 858-874
Protein Sequence>Plasma membrane ATPase 1 MTDTSSSSSSSSASSVSAHQPTQEKPAKTYDDAASESSDDDDIDALIEELQSNHGVDDED SDNDGPVAAGEARPVPEEYLQTDPSYGLTSDEVLKRRKKYGLNQMADEKESLVVKFVMFF VGPIQFVMEAAAILAAGLSDWVDFGVICGLLMLNAGVGFVQEFQAGSIVDELKKTLANTA VVIRDGQLVEIPANEVVPGDILQLEDGTVIPTDGRIVTEDCFLQIDQSAITGESLAVDKH YGDQTFSSSTVKRGEGFMVVTATGDNTFVGRAAALVNKAAGGQGHFTEVLNGIGIILLVL VIATLLLVWTACFYRTNGIVRILRYTLGITIIGVPVGLPAVVTTTMAVGAAYLAKKQAIV QKLSAIESLAGVEILCSDKTGTLTKNKLSLHEPYTVEGVSPDDLMLTACLAASRKKKGLD AIDKAFLKSLKQYPKAKDALTKYKVLEFHPFDPVSKKVTAVVESPEGERIVCVKGAPLFV LKTVEEDHPIPEDVHENYENKVAELASRGFRALGVARKRGEGHWEILGVMPCMDPPRDDT AQTVSEARHLGLRVKMLTGDAVGIAKETCRQLGLGTNIYNAERLGLGGGGDMPGSELADF VENADGFAEVFPQHKYRVVEILQNRGYLVAMTGDGVNDAPSLKKADTGIAVEGATDAARS AADIVFLAPGLSAIIDALKTSRQIFHRMYSYVVYRIALSLHLEIFLGLWIAILDNSLDID LIVFIAIFADVATLAIAYDNAPYSPKPVKWNLPRLWGMSIILGIVLAIGSWITLTTMFLP KGGIIQNFGAMNGIMFLQISLTENWLIFITRAAGPFWSSIPSWQLAGAVFAVDIIATMFT LFGWWSENWTDIVTVVRVWIWSIGIFCVLGGFYYEMSTSEAFDRLMNGKPMKEKKSTRSV EDFMAAMQRVSTQHEKET
References
External Links
ResourceLink
Saccharomyces Genome Database PMA1
Uniprot IDP05030
Uniprot NamePMA1_YEAST
GenBank Gene IDX03534
Genebank Protein ID4187
General Reference
  • Serrano, R., Kielland-Brandt, M. C., Fink, G. R. (1986). "Yeast plasma membrane ATPase is essential for growth and has homology with (Na+ + K+), K+- and Ca2+-ATPases." Nature 319:689-693.3005867
  • Tettelin, H., Agostoni Carbone, M. L., Albermann, K., Albers, M., Arroyo, J., Backes, U., Barreiros, T., Bertani, I., Bjourson, A. J., Bruckner, M., Bruschi, C. V., Carignani, G., Castagnoli, L., Cerdan, E., Clemente, M. L., Coblenz, A., Coglievina, M., Coissac, E., Defoor, E., Del Bino, S., Delius, H., Delneri, D., de Wergifosse, P., Dujon, B., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Nature 387:81-84.9169869
  • Davis, C. B., Smith, K. E., Campbell, B. N. Jr, Hammes, G. G. (1990). "The ATP binding site of the yeast plasma membrane proton-translocating ATPase." J Biol Chem 265:1300-1305.2136852
  • Portillo, F., Serrano, R. (1988). "Dissection of functional domains of the yeast proton-pumping ATPase by directed mutagenesis." EMBO J 7:1793-1798.2901955
  • Ficarro, S. B., McCleland, M. L., Stukenberg, P. T., Burke, D. J., Ross, M. M., Shabanowitz, J., Hunt, D. F., White, F. M. (2002). "Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Nat Biotechnol 20:301-305.11875433
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Hitchcock, A. L., Auld, K., Gygi, S. P., Silver, P. A. (2003). "A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery." Proc Natl Acad Sci U S A 100:12735-12740.14557538
  • Gruhler, A., Olsen, J. V., Mohammed, S., Mortensen, P., Faergeman, N. J., Mann, M., Jensen, O. N. (2005). "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Mol Cell Proteomics 4:310-327.15665377
  • Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258
  • Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
  • Chi, A., Huttenhower, C., Geer, L. Y., Coon, J. J., Syka, J. E., Bai, D. L., Shabanowitz, J., Burke, D. J., Troyanskaya, O. G., Hunt, D. F. (2007). "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Proc Natl Acad Sci U S A 104:2193-2198.17287358
  • Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956