Oxysterol-binding protein homolog 2 (Q12451)

Identification
NameOxysterol-binding protein homolog 2
SynonymsNot Available
Gene NameOSH2
Enzyme ClassNot Available
Biological Properties
General FunctionInvolved in protein binding
Specific FunctionLipid-binding protein involved in maintenance of intracellular sterol distribution and homeostasis. Binds to phosphoinositides
Cellular LocationCell membrane; Peripheral membrane protein.
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
GO Classification
Process
steroid metabolic process
metabolic process
primary metabolic process
lipid metabolic process
Gene Properties
Chromosome LocationNot Available
Locus
Gene Sequence>3852 bp ATGTCTAGGGAAGACTTGTCCATTGCTGAAGATCTCAACCAAGTGAGCAAACCTTTACTC AAAGTGAAGCTGCTGGAAGTTTTGGGTCAGGGGGATTTCAAACATTTAAAGGCGCTTGTG GACAATGAATTTCAACCTAAAGACGACCCATCTGTACAGCAAGTTCTTAATTTAATTTTA CATTATGCTGTCCAGGTGGCCCCTATTCTTCTTATCAAGGAAATTGTTGCTCATTGGGTG GACCAAGTAGGTGATGAGAAGAGTTCGAGTAAAAGCGATGATGGTATCCATCTAGATTTG AATTATCAGGATGAAAATGGCAATACTCCATTGCACTTGGCTGCTGCTCAATCTCGGTCG GATGTGATCAGTTTCCTTCTTAGCCAGAAGTCTATCAATGATTGTGTCAAGAATAAAGCT CATCAGCAGCCTTTGGACATGTGCAAAGATTTGAATGTAGCACAAATGATTCAATTGAAA AGAGATGACTATTTTTTGGAAACAGTTCATTCTTTGAGAGCAGCCATGAATAAAAGAGAC TTTTCGAAATTGGACTCTATTTGGAAAAATCCAAGAAACCTAAATTTACTAGATATTAAT GGCATAGATCCTGAAACCGGAACGACACTTCTGTATGAATATTCGCAAAAAAAAGACATT GAAATGTGTCAATGGCTGCTGAAACATGGTGCAGAAGCAACAGTTAAAGATGGGAAGGGA CGGTCGCCATTGGATTTAGTCAAAAATATAAAACTACCCGCTAAACCGTCGAACAACGTT ACACCGGAAATTAAGTTAAAGAATCTACTTGAAAAGAATCTGAGAGAACAAGCTATTGTT CACGAAGATGTCGCATCAAGTAAGCCTCCTACGTATAAGGGGTTTTTAAAGAAATGGACA AATTTTGCCCATGGCTACAAATTACGTTGGTTCATTTTAAGTGGCGACGGAAATCTGTCA TATTACAAAGACCAATCGCATGTTGATAGGCCTCGTGGTACTTTAAAAGTTTCGACGTGC CGCTTACACATCGATTCTTCAGAAAAACTGAATTTCGAGTTGCTAGGTGGCATCACAGGC ACAACAAGGTGGCGCTTAAAGGGCAATCATCCCATTGAAACGACTAGGTGGGTCAATGCT ATACAAAGCGCTATTAGATTCGCCAAAGACAAGGAGATATTAAACAAAAAAAAGGCAGTA CCACCATCATTGGCCTTGAAGAACAAATCTCCAGCCCTGATATCGCATTCCAAAACACAA GGATCTCTTCCTGAAGCTTCGCAATACTATCAGCACACATTACACAAGGAAGTTATTCAG CCCTCCTCTGTGAGTCTTTATCGGAGACCAAGCAACAACCTGTCCGTCGTATCCAGCGAA ATCCAACTAAACGATAATTTGACTGAATCTGGCAAGCGCTTTGTTAGTAAAATGATTGAA AACCGGCTGGATGGTAGCAAAACCCCCGTTGGAGTACACACAGGTTCAGCTCTGCAAAGG GTCCGCAGTAGTAATACCTTGAAAAGTAACCGGAGTATGCAAAGTGGCTCAGGTGTAGCT AGTCCTATAGATAAAGTCCCTAACGGTGCGAATTTGAGCCAGTCTAACACCACAACAGGA TCTACTGCTTCCTTGAGTGATAATAACTATATTGATAATTTCGAGGGTGATGAGGCAAAT TCAGATGATGAAGAGGAAGACCTTGGAATAAATTTTGATCGTGATGAAGAATACATCAAA GCACAGTATGGGCCATATAAGGAGAAACTTGATATGTATGAACAAGCAATCAGTATAGAG TTGAGTTCTTTGATTGAACTAATTGAACAAGAGGAGCCAAGTCCTGAGGTTTGGCTCACA ATAAAGAAGAGCCTCATCAACACATCAACCATTTTTGGTAAGTTGAAGGACTTGACTTAT AAAAGAGACAAAAGATTAGTGGATATGGTTTCAAAGCAAGGTGATGTCAATAACGTTTGG GTGCAATCTGTGAAAGAATTAGAAATGGAGTTGTCCAACAAAACCGAACGTCTAGCATCT ATTGATAAAGAAAGAAGAGGTTTGAAAAAGATACTACACAAGAAATTGCTGGAATCACAT GCCACTGCCGGCAACAAGGAATCCTTAGAAAATGACAAAGAACAGGAATCAGATACTACG GCAAGTACTCTCGGTCAAATTGCGAAATTTATCAGCGCTACAAAGGAAGAAGATGAAGCA TCTGATGCTGATGAGTTTTATGATGCCGCGGAATTGGTGGATGAAGTAACTGAATTAACA GAGGCTCATCCTGAAATTTCGACCGCTGCAGCTCCCAAGCATGCTCCTCCCCCAGTTCCA AATGAAACAGATAACGACAGCCAGTATGTCCAGGACGAAAAAAGCAAAATAGAGTCAAAT GTTGAGAAAACAAGCCAAAAATTTGAAAAACAAAATAATCTAGTTACAGAAGATGAACCA AAAACTGATCAATCACTGAAAAACTTCAAAGCGGAGGACAAAGAGAGCCAAGTAAAGGAG AAAACTAAAGAGATAGCATCTTCAGTTATCGGTGAAAAGACCATAGTTGCTGTCACTACA GTGCAAAAAAGAAAAGAAGAATATTTACTCAAAGAAGGGTCCTATCTTGGTTATGAAGAT GGTATTAGAAAAAGACTATCTATGGATAAGGACGATAGACCAAAGATTAGTCTTTGGGCC GTTCTGAAATCGATGGTGGGCAAAGATATGACAAGAATGACGTTACCAGTTACTTTCAAT GAGCCTACATCTCTTTTACAAAGAGTGGCAGAGGATTTGGAGTATTCCGAACTATTAGAC CAGGCTGCAACGTTTGAAGATTCAACACTGAGAACTCTATATGTTGCGGCATTTACCGCA TCTTCATACGCATCTACTACAAAAAGAGTTGCGAAACCCTTCAACCCATTGTTGGGTGAA ACATTTGAGTATTCTCGACCAGATAAACAGTACAGATTTTTCACCGAGCAGGTTTCACAT CACCCTCCTATATCTGCAACATGGACTGAATCACCAAGATGGGACTTTTGGGGTGAATCA TTTGTTGATACTAAATTCAATGGCAGATCTTTCAATGTTAAGCACTTAGGATTATGGCAC ATTAAGTTGCGTCCAAATGACAATGAAAAAGAGGAACTGTATACTTGGAAGAAGCCCAAC AATACTGTTATAGGTATTCTCATTGGTAATCCACAAGTTGATAACCACGGTGAAGTGAAC GTTGTCAATCACACAACCGGTGATCATTGCAAGTTGTATTTCAAAGCAAGGGGATGGAGG TCATCAGGAGCATATGAAATCACAGGCGAAGTCTACAACAAGAAAAAACAGAAGGTGTGG ATCCTAGGAGGACACTGGAATGAAGCTATTTTTGCCAAGAAAGTAGTAAAAGATGGCGAT CTCTCGTTGGAGAAAACTAGAACCGCTGCATCAGCAGGGAATGGGCCTACGGATGACGGA ACGAAGTTTTTAATATGGAAGGCAAACGACCGTCCTGAGGAGCCATTCAATTTAACACCT TTTGCTATCACGCTGAATGCTCCACAACCACATCTATTGCCATGGTTACCCCCCACAGAT ACACGTTTGAGACCAGATCAAAGAGCAATGGAAGATGGGCGTTATGACGAAGCTGGTGAT GAGAAATTCAGAGTTGAAGAAAAGCAAAGAGCAGCACGCAGAAAAAGGGAGGAAAATAAC CTTGAATATCACCCACAATGGTTCGTCAGGGATACTCATCCCATCACAAAAGCTAAATAT TGGAGATATACGGGCAAATATTGGGTTAAAAGAAGAGATCATGATTTGAAAGATTGTGGT GACATTTTTTAA
Protein Properties
Pfam Domain Function
Protein Residues1283
Protein Molecular Weight145795.0
Protein Theoretical pI6.81
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Oxysterol-binding protein homolog 2 MSREDLSIAEDLNQVSKPLLKVKLLEVLGQGDFKHLKALVDNEFQPKDDPSVQQVLNLIL HYAVQVAPILLIKEIVAHWVDQVGDEKSSSKSDDGIHLDLNYQDENGNTPLHLAAAQSRS DVISFLLSQKSINDCVKNKAHQQPLDMCKDLNVAQMIQLKRDDYFLETVHSLRAAMNKRD FSKLDSIWKNPRNLNLLDINGIDPETGTTLLYEYSQKKDIEMCQWLLKHGAEATVKDGKG RSPLDLVKNIKLPAKPSNNVTPEIKLKNLLEKNLREQAIVHEDVASSKPPTYKGFLKKWT NFAHGYKLRWFILSGDGNLSYYKDQSHVDRPRGTLKVSTCRLHIDSSEKLNFELLGGITG TTRWRLKGNHPIETTRWVNAIQSAIRFAKDKEILNKKKAVPPSLALKNKSPALISHSKTQ GSLPEASQYYQHTLHKEVIQPSSVSLYRRPSNNLSVVSSEIQLNDNLTESGKRFVSKMIE NRLDGSKTPVGVHTGSALQRVRSSNTLKSNRSMQSGSGVASPIDKVPNGANLSQSNTTTG STASLSDNNYIDNFEGDEANSDDEEEDLGINFDRDEEYIKAQYGPYKEKLDMYEQAISIE LSSLIELIEQEEPSPEVWLTIKKSLINTSTIFGKLKDLTYKRDKRLVDMVSKQGDVNNVW VQSVKELEMELSNKTERLASIDKERRGLKKILHKKLLESHATAGNKESLENDKEQESDTT ASTLGQIAKFISATKEEDEASDADEFYDAAELVDEVTELTEAHPEISTAAAPKHAPPPVP NETDNDSQYVQDEKSKIESNVEKTSQKFEKQNNLVTEDEPKTDQSLKNFKAEDKESQVKE KTKEIASSVIGEKTIVAVTTVQKRKEEYLLKEGSYLGYEDGIRKRLSMDKDDRPKISLWA VLKSMVGKDMTRMTLPVTFNEPTSLLQRVAEDLEYSELLDQAATFEDSTLRTLYVAAFTA SSYASTTKRVAKPFNPLLGETFEYSRPDKQYRFFTEQVSHHPPISATWTESPRWDFWGES FVDTKFNGRSFNVKHLGLWHIKLRPNDNEKEELYTWKKPNNTVIGILIGNPQVDNHGEVN VVNHTTGDHCKLYFKARGWRSSGAYEITGEVYNKKKQKVWILGGHWNEAIFAKKVVKDGD LSLEKTRTAASAGNGPTDDGTKFLIWKANDRPEEPFNLTPFAITLNAPQPHLLPWLPPTD TRLRPDQRAMEDGRYDEAGDEKFRVEEKQRAARRKREENNLEYHPQWFVRDTHPITKAKY WRYTGKYWVKRRDHDLKDCGDIF
References
External Links
ResourceLink
Saccharomyces Genome Database OSH2
Uniprot IDQ12451
Uniprot NameOSH2_YEAST
GenBank Gene IDZ48432
Genebank Protein ID683678
General Reference
  • Jacq, C., Alt-Morbe, J., Andre, B., Arnold, W., Bahr, A., Ballesta, J. P., Bargues, M., Baron, L., Becker, A., Biteau, N., Blocker, H., Blugeon, C., Boskovic, J., Brandt, P., Bruckner, M., Buitrago, M. J., Coster, F., Delaveau, T., del Rey, F., Dujon, B., Eide, L. G., Garcia-Cantalejo, J. M., Goffeau, A., Gomez-Peris, A., Zaccaria, P., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Nature 387:75-78.9169867
  • Levine, T. P., Munro, S. (2001). "Dual targeting of Osh1p, a yeast homologue of oxysterol-binding protein, to both the Golgi and the nucleus-vacuole junction." Mol Biol Cell 12:1633-1644.11408574
  • Beh, C. T., Cool, L., Phillips, J., Rine, J. (2001). "Overlapping functions of the yeast oxysterol-binding protein homologues." Genetics 157:1117-1140.11238399
  • Loewen, C. J., Roy, A., Levine, T. P. (2003). "A conserved ER targeting motif in three families of lipid binding proteins and in Opi1p binds VAP." EMBO J 22:2025-2035.12727870
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Beh, C. T., Rine, J. (2004). "A role for yeast oxysterol-binding protein homologs in endocytosis and in the maintenance of intracellular sterol-lipid distribution." J Cell Sci 117:2983-2996.15173322
  • Yu, J. W., Mendrola, J. M., Audhya, A., Singh, S., Keleti, D., DeWald, D. B., Murray, D., Emr, S. D., Lemmon, M. A. (2004). "Genome-wide analysis of membrane targeting by S. cerevisiae pleckstrin homology domains." Mol Cell 13:677-688.15023338
  • Chi, A., Huttenhower, C., Geer, L. Y., Coon, J. J., Syka, J. E., Bai, D. L., Shabanowitz, J., Burke, D. J., Troyanskaya, O. G., Hunt, D. F. (2007). "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Proc Natl Acad Sci U S A 104:2193-2198.17287358
  • Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956