Endo-1,3(4)-beta-glucanase 2 (Q12168)

Identification
NameEndo-1,3(4)-beta-glucanase 2
Synonyms
  • Endo-1,3-beta-glucanase 2
  • Endo-1,4-beta-glucanase 2
  • Laminarinase-2
Gene NameACF2
Enzyme Class
Biological Properties
General FunctionInvolved in endo-1,3(4)-beta-glucanase activity
Specific FunctionEndohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3
Cellular LocationCytoplasm
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00890waterShow
GO Classification
Component
Not Available
Function
endo-1,3(4)-beta-glucanase activity
catalytic activity
hydrolase activity
hydrolase activity, acting on glycosyl bonds
hydrolase activity, hydrolyzing O-glycosyl compounds
Process
catabolic process
cell wall macromolecule catabolic process
metabolic process
Gene Properties
Chromosome Locationchromosome 12
LocusYLR144C
Gene Sequence>2340 bp ATGTGTTACAGTAGGCAAGCCATTCCTCCACCTGTTCCTAATAGACCTGGCGGTACTACC AATAGAGGACCTCCTCCCTTACCACCTCGCGCAAATGTTCAGCCACCAGTTTGTTCTTCT GAGAACTCCAGTAAACCTCGAGAAAATAGGGTGGCAGGTGAATCACTGCGCACACCAAGC AGTTCTAACCCTTTAGCTGACAGCCAGGTGAATAGTGACAATATTTTTCAATCACCTGTC CTTTCTAACTTAAAAGCTCCTCCTTCCGTCTTCAACAAAGTTCAACACCCGGTACCAAAG CCGAATATTGATGATCAATCTGTTGATCCATTGGAGACAAACAAGTTCTACACAAACATG TTATTAGACGATAATACACAACCTATATGGACACATCCTTATTCAATTTGGTTTTCTCGT GATCCAGAATTGTTTGGTTTGGCCGCCAATCATACTTTAGCGTCTCAAAGGGTTTTTGAT ACAACTACAAATCCTCCCAGATTCTACTTCAATCCCACAAATATAAAATCATTTGTATTC AAGGCCAGGGAATTTGTCTCTTCAAATGATATCAAGCTTGAATTCCGGGATATGAAACAT ATGTCCATGTGCTTACTAATGTCTTTGAGTAGTTCCCAGTTTATAGAATTCCCGCTGGTT CAGGGGATGGGCTTTGTCACGGCTATTTACCACGATTTGGGTTTTGAATTAAGAAGCGCC GTTGGTTTCAGGAGCTTAGAGCGCATAAGCGTGAATGAAAGATATGGTAAATATAATATA CAACTTGAGAATAATAGGAATTGGATACTTTATTTGACCTCACCTGATTACTCTTTTCCA CAAGATTTTCAAATTTCATTACTCGACAGCAATACAATAATATCATCTCATAAAATCAAT GGTCTTATCTGTCAATTATCTGCAGACTCCGTCCCCAGTATTGACATGGCAGCGGGCTGT TATCCAGTATACTGTGACCTATCAGGGCAAACTGTTGATGAACATTTCACAAATTACAGA TTTAACTATACGGTTGCAGGCTACTCGCAGTCTGGAACTACTCTAATGTATGCTTTACCA CATCATAAAGCGGCATTCACACCAGAAATGCAGGAACGTGAAATTGCCTCAAGCCTGGAT TCCACAGTTAAAGGCTTAATGACTGGTTATCTCACAAACAGTTTTGATATGCAGGTTCAA GTACCTCAAGAATTGGGCTTTGAACCTGTCGCGTTATCTTTGAATAAGAAGGCAGATTAC AGTCAAGAAAAGCTATCTAAAATTCGGGAAGCTGCTGTTCAAGAAGTTCAATTGAGTGAC CCTCAACAAGAATCAAACATAGATTCTATGTATTTCTCTGGTAAGATACTTGCAAAGTAT GCGTGGATTCTTTATGTGACACATTACATCCTTCATGATGAAAATTTAACAAAAGAATTA TTATCTAAACTGACTATTGCAATGGAGAGATTCATCAGCAATCAGCAAGTCTTACCTTTA AATTACGACGTTAGTTGGAAAGGCATAATTTCATCAGGCTCTTCTAGCCAAGACTTTGGT AACTCTTATTATAATGATCATCATTTTCATTACTCTTATCATGTCATAACGGCTGCCATT ATCTCTTTAGTCGATTCTGATTTAAGTGGAGTTACTAACAACTCCTGGTTAGAAAATAAT AGAGATTGGGTTGAGTGCTTAATTAGAGATTACTCTGGTGTTGACAACGATGATCCGTAC TTCCCTCAATTTAGGTCATTTGATTGGTTTAATGGCCATTCATGGGCCAAAGGACTATTT CCAAGTGGTGACGGTAAAGATGAAGAATCTACTTCAGAAGATGTCAATTCTTGCTATGCG ATAAAATTGTGGGGGTTGGTAACTGGAAATTCTAAGTTAACTGACATAGCGAACTTACAA CTCGGCATCATGAGAAATGTTTTCCAGAGCTACTTCCTATACGAAAGCAATAATACTGTC CAACCGAAAGAATTTATTGGTAATAAAGTTAGTGGTATTTTATTCGAAAATAAAATTGAT CACGCCACGTATTTTGGCATGGAGCCTCAATACATTCATATGATTCACGCCATTCCTATA ACATCAGCATCTTCATGGGTCAGAACACCAAATTTTGTCAAAGAGGAGTGGGAAGAAAAA ATGCAGCCGATAATTGATCAAGTGAATGACGGTTGGAAAGGAATAATCATGTTAAATATG GCTCTGCTTGATCCAAAATTTTCGTACGACTTTTTCAGCCAACCTGATTTCAACAGAAAT TTCCTAGACAATGGGCAAAGCTTAACTTGGTCTTTAGCTTATTCAGGTGCTTTTTCTTAG
Protein Properties
Pfam Domain Function
Protein Residues779
Protein Molecular Weight88402.5
Protein Theoretical pI5.07
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Endo-1,3(4)-beta-glucanase 2 MCYSRQAIPPPVPNRPGGTTNRGPPPLPPRANVQPPVCSSENSSKPRENRVAGESLRTPS SSNPLADSQVNSDNIFQSPVLSNLKAPPSVFNKVQHPVPKPNIDDQSVDPLETNKFYTNM LLDDNTQPIWTHPYSIWFSRDPELFGLAANHTLASQRVFDTTTNPPRFYFNPTNIKSFVF KAREFVSSNDIKLEFRDMKHMSMCLLMSLSSSQFIEFPLVQGMGFVTAIYHDLGFELRSA VGFRSLERISVNERYGKYNIQLENNRNWILYLTSPDYSFPQDFQISLLDSNTIISSHKIN GLICQLSADSVPSIDMAAGCYPVYCDLSGQTVDEHFTNYRFNYTVAGYSQSGTTLMYALP HHKAAFTPEMQEREIASSLDSTVKGLMTGYLTNSFDMQVQVPQELGFEPVALSLNKKADY SQEKLSKIREAAVQEVQLSDPQQESNIDSMYFSGKILAKYAWILYVTHYILHDENLTKEL LSKLTIAMERFISNQQVLPLNYDVSWKGIISSGSSSQDFGNSYYNDHHFHYSYHVITAAI ISLVDSDLSGVTNNSWLENNRDWVECLIRDYSGVDNDDPYFPQFRSFDWFNGHSWAKGLF PSGDGKDEESTSEDVNSCYAIKLWGLVTGNSKLTDIANLQLGIMRNVFQSYFLYESNNTV QPKEFIGNKVSGILFENKIDHATYFGMEPQYIHMIHAIPITSASSWVRTPNFVKEEWEEK MQPIIDQVNDGWKGIIMLNMALLDPKFSYDFFSQPDFNRNFLDNGQSLTWSLAYSGAFS
References
External Links
ResourceLink
Saccharomyces Genome Database ACF2
Uniprot IDQ12168
Uniprot NameENG2_YEAST
GenBank Gene IDU53879
Genebank Protein ID1262304
General Reference
  • Baladron, V., Ufano, S., Duenas, E., Martin-Cuadrado, A. B., del Rey, F., Vazquez de Aldana, C. R. (2002). "Eng1p, an endo-1,3-beta-glucanase localized at the daughter side of the septum, is involved in cell separation in Saccharomyces cerevisiae." Eukaryot Cell 1:774-786.12455695
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Mrsa, V., Klebl, F., Tanner, W. (1993). "Purification and characterization of the Saccharomyces cerevisiae BGL2 gene product, a cell wall endo-beta-1,3-glucanase." J Bacteriol 175:2102-2106.8458852
  • Johnston, M., Hillier, L., Riles, L., Albermann, K., Andre, B., Ansorge, W., Benes, V., Bruckner, M., Delius, H., Dubois, E., Dusterhoft, A., Entian, K. D., Floeth, M., Goffeau, A., Hebling, U., Heumann, K., Heuss-Neitzel, D., Hilbert, H., Hilger, F., Kleine, K., Kotter, P., Louis, E. J., Messenguy, F., Mewes, H. W., Hoheisel, J. D., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Nature 387:87-90.9169871
  • Martin-Cuadrado, A. B., Fontaine, T., Esteban, P. F., del Dedo, J. E., de Medina-Redondo, M., del Rey, F., Latge, J. P., de Aldana, C. R. (2008). "Characterization of the endo-beta-1,3-glucanase activity of S. cerevisiae Eng2 and other members of the GH81 family." Fungal Genet Biol 45:542-553.17933563
  • Cappellaro, C., Mrsa, V., Tanner, W. (1998). "New potential cell wall glucanases of Saccharomyces cerevisiae and their involvement in mating." J Bacteriol 180:5030-5037.9748433