GPI ethanolamine phosphate transferase 3 (Q07830)

Identification
NameGPI ethanolamine phosphate transferase 3
Synonyms
  • Glycosylphosphatidylinositol-anchor biosynthesis protein 13
Gene NameGPI13
Enzyme ClassNot Available
Biological Properties
General FunctionInvolved in mannose-ethanolamine phosphotransferase act
Specific FunctionInvolved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the GPI third mannose which links the GPI-anchor to the C-terminus of the proteins by an amide bond. Involved in cell wall biosynthesis
Cellular LocationEndoplasmic reticulum membrane; Multi-pass membrane protein (Potential)
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG Reactions
PE(14:0/16:1(9Z)) + 6-O-{alpha-D-mannosyl-(1->2)-alpha-D-mannosyl-(1->6)-2-O-[(2-aminoethyl)phosphoryl]-alpha-D-mannosyl-(1->4)-alpha-D-glucosaminyl}-O-acyl-1-phosphatidyl-1D-myo-inositol ↔ DG(16:0/16:0/0:0) + 6-[6-(2-aminoethylphosphoryl)-alpha-mannosyl-(1->6)-2-(2-aminoethylphosphoryl)-alpha-mannosyl-(1->6)-alpha-2-(2-aminoethylphosphoryl)mannosyl-(1->4)-alpha-glucosaminyl]-O-acyl-1-phosphatidyl-1D-myo-inositol
Metabolites
YMDB IDNameView
GO Classification
Function
catalytic activity
Process
metabolic process
Gene Properties
Chromosome LocationNot Available
Locus
Gene Sequence>3054 bp ATGGATGAAAAGACAATTAAAAAGTCGATTCTTTCATCTTCCAATGATGAAAAAATAATA TACAAATCGCGAATAAAGAAATTTCAAAAGAATCATAAATTTTATATTATTCTATTGGTT TTCATCGCCATTTTACAATTCATATCAATAGCTTTTTTCACTCGGGGATTCTTACTGTCA AGACACGTTCTTGATAATATTTCCTCACAAAACGAAACGAGTAAGTTACCTCCTAGGTTC AACAAGGCGGTGATTTTGGTTATTGACGCTTTGAGATTTGATTTCGCTATTCCTGTAAAC GAATCCCACTCTAATTACAACTTGAACTATCATAATAATATATTATCATTGTATGATTCT TTTGCAAGCGATAAGGATGCATCTTCCCTATTGTTGAAGTTCATTGCCGACCCACCAACC ACTACTTTACAAAGACTCAAGGGTCTCACAACAGGGTCCTTACCGACTTTCATTGATGCC GGGTCTAACTTCGATGGAACAGTAATCGAAGAGGATAACTTTCTTAAACAGCTCCATTTG GCAAATAAGACTGTCAAATTTGCCGGAGATGATACATGGATGGCTCTGTTCCATCCGTTC CTTTCCAACGATAGTTTCCCACTAGAATCACTCAATGTGTGGGACTTAGATACTGTCGAT AACGGTGTTATGGATTACTTCCATGATCATTTACAGCAGGACAAGGAATGGGACGTCATG ATTGGACACATGCTAGGTATTGACCATGTGGGCCACAAATATGGCCCCGACCATTTTACT ATGAGGGAAAAACAAATTCAAGTAGATCAATTTATCGATTGGATACTAAAATCGATCGAT GATGATACCTTATTGGTTATTTTGGGTGACCATGGAATGGACCATACGGGCAATCACGGT GGCGACTCCATCGACGAGCTCGAAAGCACTTTATTTCTTTATTCTAAGAAGCCGGACATG TGGAGACTTAAAGAAACTTCGAATTACAACATTGATAATTTGGGACACGATTACCGTTCA GTGAGACAAATTGATTTGGTGTCTAGTTTGGCGTTATTGATGGGCCAACCGATACCATTT AACAATCTTGGATGGCCTATTGACGAAATAGCAAGAAACGATAGAGAATGGTCTCAATTT GTCAACTCAGCGATAAGCCAATTACAATTATATAAGGACACGATGCAGATCCACCATGGC AATGACGAAATATTGGAACCGTTAGCGAAAAATATATCTAACACCCCCCCAACAAGCGAT CCAGAAAAATTTGTTAAGCTGGGTCATAAATACCAAAAGGTCTTCTTGCAGACCTGCGAA GAACTATGGGCCAAATTCGATTATTATTCCATTGCCACCGGTATAACGCTACTGGCCACT TCACTGGTCCTGTTGATATCCATTACAAAATTAATTCCTTCTATTGTTGTGAATCAAATG GTTCCAGAATTCGTTCCGGGAATTATCATAATGGTCTTAGTTACAAACCTATGTTTCCAT GGAATTTTTTACGTTTATCAACAACCAAGTTTTGTAGACCAATTCTGGGGTACCCTGTTA GCGACTGCAATTGGTATCATTATTGGTTGTTATATCACAATATTTGATAGGTACAACTTT ATTTGGATTGCTATGAGACTGGGTGAAACATTAGCTGATTATTGGTCAAGAATTGCTGTC ATGTTTATGATTATTCATGCTTTGCTTTTCACTTCCAATTCATTTACTATTTGGGAGGAT AGAATTGTAGCATTCCTTTTATCTACTTTCGGCATGTTGACGTTGTATGAATTTGTCTTC TTACCAAAAAGGCAATCCACCACTGCATTATTAACTGCAACAATCAGTGAAAAGGAAGGA ACCACTTCAGGGGTCAATCCTTCGACAGCAAATTCAAATTACTTACCATTAACAAGATTT GCAAGATTACTAGGAGGTTACCATTCTGCAGTTTTAATCATTTTTACCCGGTTAGCTTCT ATGATTACCATTTGTAGAGAGGAACAAGGCGAATATTGTATTCCAACATTTAATAATCAA AACAATTCCTCGTGGTGGGTTTTGGGTCTCTGTTTCCTAATGATATTCATATTACCTGCA TGCATCACCGGTTATTATAATCTAACATCGTCTTATCAAGCTGCAGCTCCAATTTGGATA AACGTTTTCCTAAAGGGTATCCTTGGTTTGAACTTTGTTTATTGGTCATTGACTTCATTA GAAAATAACAGTGCGGTAATAGCAATACCGTTCTTACGGGACGTCACTATTTTTAAATTT ACGTTGGCTAGAATTATTGCCGGTTTTTCATTGATTGCTTCTAATGTCGGCTGGCTAATG GGACCACTTTGTATTAAATTAAACATTCATAATACTGATGTTAAGTCTCACGAAGCAACA ATTTTAGGTTATACTAATATTTACGGCTCAGAGTTTTTTCTTTTGGTCATAAACGTTCTC ATTAGTATCCTACTATTCAACAAACCCCTGGCCCAGTTGTCATACTTCCTCATGTGCAAC CAACTACTATCAATTTTAGAAATTATCGATCTTTTAAAATTAAAGGAAAACATAATTGGT CCAATTGCGTTGGGACTCTTATCTTACCAACATTTTTTCACCACCGGTCATCAAGCCACA ATTCCATCAGTGCAATGGGATATCGGATTTATGCTGTCTGAAAAGGTAACGTTCCCCTTC ACTCAGATAGCCATCATCCTAAATACATTTGGGCCTCATATTCTCGTCTCCTTATCTGTA GCGTTACTGACACTGTGGTCACAGCCTCCTGATGTGTTGAAGCCACAAACACTTTTAGGA AGAATTGTGTCAAACTGCGGTATATTACTCACTTACAATACTATTTTATGTCTAAGCTCC TTTATTTGGGTTACTCACTTCCGTAGACACTTGATGGTTTGGAAAATCTTCTGCCCAAGG TTTATCTTTGCTTCTTTAAGTTTAATAGTCACTCAACTGGTCGTTACTTTTGGTACAATT GCATTTGCTAGTGGGAGATTGATTAAGCACATAAATGACATTTTTTGGAAATGA
Protein Properties
Pfam Domain Function
Protein Residues1017
Protein Molecular Weight115633.0
Protein Theoretical pI6.62
Signalling Regions
  • None
Transmembrane Regions
  • 34-54
  • 347-367
  • 457-477
  • 484-504
  • 515-535
  • 558-578
  • 582-602
  • 644-664
  • 685-705
  • 715-735
  • 765-785
  • 806-826
  • 829-849
  • 903-923
  • 947-967
  • 981-1001
Protein Sequence>GPI ethanolamine phosphate transferase 3 MDEKTIKKSILSSSNDEKIIYKSRIKKFQKNHKFYIILLVFIAILQFISIAFFTRGFLLS RHVLDNISSQNETSKLPPRFNKAVILVIDALRFDFAIPVNESHSNYNLNYHNNILSLYDS FASDKDASSLLLKFIADPPTTTLQRLKGLTTGSLPTFIDAGSNFDGTVIEEDNFLKQLHL ANKTVKFAGDDTWMALFHPFLSNDSFPLESLNVWDLDTVDNGVMDYFHDHLQQDKEWDVM IGHMLGIDHVGHKYGPDHFTMREKQIQVDQFIDWILKSIDDDTLLVILGDHGMDHTGNHG GDSIDELESTLFLYSKKPDMWRLKETSNYNIDNLGHDYRSVRQIDLVSSLALLMGQPIPF NNLGWPIDEIARNDREWSQFVNSAISQLQLYKDTMQIHHGNDEILEPLAKNISNTPPTSD PEKFVKLGHKYQKVFLQTCEELWAKFDYYSIATGITLLATSLVLLISITKLIPSIVVNQM VPEFVPGIIIMVLVTNLCFHGIFYVYQQPSFVDQFWGTLLATAIGIIIGCYITIFDRYNF IWIAMRLGETLADYWSRIAVMFMIIHALLFTSNSFTIWEDRIVAFLLSTFGMLTLYEFVF LPKRQSTTALLTATISEKEGTTSGVNPSTANSNYLPLTRFARLLGGYHSAVLIIFTRLAS MITICREEQGEYCIPTFNNQNNSSWWVLGLCFLMIFILPACITGYYNLTSSYQAAAPIWI NVFLKGILGLNFVYWSLTSLENNSAVIAIPFLRDVTIFKFTLARIIAGFSLIASNVGWLM GPLCIKLNIHNTDVKSHEATILGYTNIYGSEFFLLVINVLISILLFNKPLAQLSYFLMCN QLLSILEIIDLLKLKENIIGPIALGLLSYQHFFTTGHQATIPSVQWDIGFMLSEKVTFPF TQIAIILNTFGPHILVSLSVALLTLWSQPPDVLKPQTLLGRIVSNCGILLTYNTILCLSS FIWVTHFRRHLMVWKIFCPRFIFASLSLIVTQLVVTFGTIAFASGRLIKHINDIFWK
References
External Links
ResourceLink
Saccharomyces Genome Database GPI13
Uniprot IDQ07830
Uniprot NameGPI13_YEAST
GenBank Gene IDZ73136
Genebank Protein ID1360216
General Reference
  • Johnston, M., Hillier, L., Riles, L., Albermann, K., Andre, B., Ansorge, W., Benes, V., Bruckner, M., Delius, H., Dubois, E., Dusterhoft, A., Entian, K. D., Floeth, M., Goffeau, A., Hebling, U., Heumann, K., Heuss-Neitzel, D., Hilbert, H., Hilger, F., Kleine, K., Kotter, P., Louis, E. J., Messenguy, F., Mewes, H. W., Hoheisel, J. D., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Nature 387:87-90.9169871
  • Zhang, N., Ismail, T., Wu, J., Woodwark, K. C., Gardner, D. C., Walmsley, R. M., Oliver, S. G. (1999). "Disruption of six novel ORFs on the left arm of chromosome XII reveals one gene essential for vegetative growth of Saccharomyces cerevisiae." Yeast 15:1287-1296.10487931
  • Flury, I., Benachour, A., Conzelmann, A. (2000). "YLL031c belongs to a novel family of membrane proteins involved in the transfer of ethanolaminephosphate onto the core structure of glycosylphosphatidylinositol anchors in yeast." J Biol Chem 275:24458-24465.10823837
  • Taron, C. H., Wiedman, J. M., Grimme, S. J., Orlean, P. (2000). "Glycosylphosphatidylinositol biosynthesis defects in Gpi11p- and Gpi13p-deficient yeast suggest a branched pathway and implicate gpi13p in phosphoethanolamine transfer to the third mannose." Mol Biol Cell 11:1611-1630.10793139
  • Toh-e, A., Oguchi, T. (2002). "Genetic characterization of genes encoding enzymes catalyzing addition of phospho-ethanolamine to the glycosylphosphatidylinositol anchor in Saccharomyces cerevisiae." Genes Genet Syst 77:309-322.12441642
  • Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106