Sterol esterase 1 (Q07804)

Identification
NameSterol esterase 1
Synonyms
  • Steryl ester hydrolase 1
Gene NameYEH1
Enzyme Class
Biological Properties
General FunctionInvolved in lipid metabolic process
Specific FunctionMediates the hydrolysis of steryl esters, thereby playing a central role in lipid metabolism. Under heme-deficient conditions, it constitutes the major steryl ester hydrolase, suggesting that it plays a central role in mobilization of steryl esters under anaerobic conditions
Cellular LocationLipid droplet. Membrane; Peripheral membrane protein
SMPDB Pathways
Steroid biosynthesisPW002482 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Steroid biosynthesisec00100 Map00100
SMPDB ReactionsNot Available
KEGG Reactions
ergosterol + fatty acid ↔ ergosterol ester + water
24-methylidene-5alpha-cholest-8-en-3beta-ol + fatty acid ↔ fecosterol ester + water
zymosterol + fatty acid ↔ zymosterol ester + water
Metabolites
YMDB IDNameView
YMDB00543ergosterolShow
YMDB0054524-methylidene-5alpha-cholest-8-en-3beta-olShow
YMDB00548zymosterolShow
YMDB00890waterShow
GO Classification
Component
Not Available
Function
Not Available
Process
metabolic process
primary metabolic process
lipid metabolic process
Gene Properties
Chromosome Locationchromosome 12
LocusYLL012W
Gene Sequence>1722 bp ATGGGTGTTTCTGCGGTGTTGAAAAGAGCTAGGAATTTACTAGCAACGTTCATAGTCTGC TGTTTTATGGCAGTAGTGCTTGTTCTGGCGCTGGCACACCATTTTATAAATGAGCACAGA GACACTAGGAGTTCATCGACCCAAATCGAAGTTGATGACGAAAGTAAAAGAAACGTACAC CATGACCATGTTCTCACTAGAACCAATGCATACGCGACGCCATACCTCGATCTGGAACAT GACAAGAAAAACGGTATCGTCTACGATCATACAAGAACGGTTGTCCGTAAAAAGAACCAC GAAGTGGGGTCCTCGTCACTGCATAAAAACCTTTTTCACAAATTTTTGACAAAGCTTATT TTTAGGTTTATCGAGAAGGAAAAAGTTACCGAAGGCGTAACGCAAGGAAAGTTCAATAAT AGTAGCAATGAAATTGCCAATCATGAACCGGTTTTTGAAAAAATTCCTGTACAGTGCGAC AATCCATTACAGAATCTAATCTTATCGGAAGACTTGACATTAGTTGCGGATCTTAATTAT TATTTTAACCAGTACAATATTCAAATAGAGGAATTTAGATTGGAGACCGAAGATGGGTTT GTTATAGATTTGTGGCACTTGATACCAAAATATAGAACGACAGATTCTGACAAGAAGAAG AGGCCACCCATTTTGATGCTACATGGCCTTTTGCAAAGCAGTGGTTCGTTCGCATCCAAT GGTAGAAAATCTCTGGCATATTTTCTGTATCAATCCGGTTACGACATATGGTTAGGGAAT AACAGATGCGGGTTTAGGCCGGAATGGAACGAAGCGAAAGTACCGACACTAGCTTCCAGG TGGGACTGGGACCTTCGCGAGATGGTTAAGTACGATCTGACCCTTTTGATTGATACCGTG TTAGCTAAGACGCAGTTTGAAAAGCTTACTTTGATCTCGCATTCTCAGGGCACTACACAG GGGTTTATGGGCTTGGTCAACGAAGATAAGTTTTTCCCTCCCGGTTCGGGATCTAAAGAA TCTTTTTTCACTTCTAAGATCGCAAACTATATTGCCTTGGCCCCCGCAGTGTATCCTGGT CCCTTACTTAACGAGAAATTGTTTGTTAAGCTTATGACAAAGGAAATCGAAAATCCCTGG TTCTTTGGTGAAACGAGCTTTTTTGAGATAATGATGATTGTAAGAAACTTGTGCGTTGGT GAGAGCTTGTTCTCCTTTGTTTGTTACACCATCTTCAATTACCTGTTTGATTGGAACGAT ACCCTTTGGGATACCGCATTAAGAGATCGCCATTTCCTGTTTTCGCCAGTCCATGTTTCA GTGAAGTTGATGCAATGGTGGCTGTCACCCGACCCCAACAAGGTAAGTTTTAAATTTGGT TCCCATAAGATGTTCCCCGACAATGTTAAGTGGTTTTCAGACGCATCAAAGGCCCCAAAT ATCTACTTGTTTGTTCCAAAGCAAGATAGATTGGTGGACGGAGAAAGACTAATCAATCAT TTCGTCAATGTGGAGTCGAATGTCAACTACAAGATCTGGTACATTGATGAGTATGCCCAT ATTGATGTCCTATGGGCACATGATGTCATAGAGAGAATTGGTAAACCAATTTTACAGAAT TTGAACAACTATTACTCCAAGAAGCCATCCAGCGCCTTTGAAAGTGATTGTTCGGACACA GAGGTGGAAACGGAGCTGGAAATGGTTGCTGAGAAGGCTTGA
Protein Properties
Pfam Domain Function
Protein Residues573
Protein Molecular Weight66507.39844
Protein Theoretical pI6.63
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Sterol esterase 1 MGVSAVLKRARNLLATFIVCCFMAVVLVLALAHHFINEHRDTRSSSTQIEVDDESKRNVH HDHVLTRTNAYATPYLDLEHDKKNGIVYDHTRTVVRKKNHEVGSSSLHKNLFHKFLTKLI FRFIEKEKVTEGVTQGKFNNSSNEIANHEPVFEKIPVQCDNPLQNLILSEDLTLVADLNY YFNQYNIQIEEFRLETEDGFVIDLWHLIPKYRTTDSDKKKRPPILMLHGLLQSSGSFASN GRKSLAYFLYQSGYDIWLGNNRCGFRPEWNEAKVPTLASRWDWDLREMVKYDLTLLIDTV LAKTQFEKLTLISHSQGTTQGFMGLVNEDKFFPPGSGSKESFFTSKIANYIALAPAVYPG PLLNEKLFVKLMTKEIENPWFFGETSFFEIMMIVRNLCVGESLFSFVCYTIFNYLFDWND TLWDTALRDRHFLFSPVHVSVKLMQWWLSPDPNKVSFKFGSHKMFPDNVKWFSDASKAPN IYLFVPKQDRLVDGERLINHFVNVESNVNYKIWYIDEYAHIDVLWAHDVIERIGKPILQN LNNYYSKKPSSAFESDCSDTEVETELEMVAEKA
References
External Links
ResourceLink
Saccharomyces Genome Database YEH1
Uniprot IDQ07804
Uniprot NameYEH1_YEAST
GenBank Gene IDX91488
Genebank Protein ID1495212
General Reference
  • Johnston, M., Hillier, L., Riles, L., Albermann, K., Andre, B., Ansorge, W., Benes, V., Bruckner, M., Delius, H., Dubois, E., Dusterhoft, A., Entian, K. D., Floeth, M., Goffeau, A., Hebling, U., Heumann, K., Heuss-Neitzel, D., Hilbert, H., Hilger, F., Kleine, K., Kotter, P., Louis, E. J., Messenguy, F., Mewes, H. W., Hoheisel, J. D., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Nature 387:87-90.9169871
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
  • Koffel, R., Tiwari, R., Falquet, L., Schneiter, R. (2005). "The Saccharomyces cerevisiae YLL012/YEH1, YLR020/YEH2, and TGL1 genes encode a novel family of membrane-anchored lipases that are required for steryl ester hydrolysis." Mol Cell Biol 25:1655-1668.15713625
  • Koffel, R., Schneiter, R. (2006). "Yeh1 constitutes the major steryl ester hydrolase under heme-deficient conditions in Saccharomyces cerevisiae." Eukaryot Cell 5:1018-1025.16835446