Oligo-1,6-glucosidase (P53051)

Identification
NameOligo-1,6-glucosidase
Synonyms
  • Alpha-glucosidase
  • Flocculent-specific protein 2
  • Isomaltase
Gene NameFSP2
Enzyme Class
Biological Properties
General FunctionInvolved in catalytic activity
Specific FunctionPreferentially hydrolyzes isomaltose, with little activity towards isomaltotriose or longer oligosaccharides. Does not hydrolyze maltose
Cellular LocationMitochondrion
SMPDB Pathways
Starch and sucrose metabolismPW002481 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Starch and sucrose metabolismec00500 Map00500
SMPDB ReactionsNot Available
KEGG Reactions
Maltose + water → D-Glucose
Metabolites
YMDB IDNameView
YMDB00286D-GlucoseShow
YMDB00584MaltoseShow
YMDB00890waterShow
GO Classification
Component
Not Available
Function
ion binding
cation binding
catalytic activity
binding
Process
metabolic process
primary metabolic process
carbohydrate metabolic process
Gene Properties
Chromosome LocationNot Available
LocusYGR287C
Gene Sequence>1770 bp ATGACTATTTCTTCTGCACATCCAGAGACAGAACCAAAGTGGTGGAAAGAGGCCACGTTC TATCAAATTTACCCAGCAAGTTTCAAAGATTCTAATGACGATGGCTGGGGTGACATGAAA GGGATTGCCTCCAAGCTGGAGTACATCAAAGAGCTTGGTGCCGATGCCATTTGGATCTCA CCATTCTACGACTCGCCACAAGATGATATGGGTTACGATATTGCCAACTACGAAAAGGTC TGGCCAACATATGGTACGAATGAAGACTGCTTTGCCTTGATCGAAAAGACACATAAGCTT GGTATGAAATTTATCACCGACTTGGTCATCAATCACTGTTCCAGCGAACATGAATGGTTC AAAGAGAGCAGATCCTCGAAGACTAATCCAAAGCGTGACTGGTTCTTCTGGAGACCTCCT AAAGGTTATGACGCCGAAGGCAAGCCAATTCCTCCAAACAATTGGAAATCCTATTTTGGT GGTTCCGCATGGACCTTCGATGAAAAGACACAAGAATTCTACTTGCGTTTGTTTTGCTCC ACTCAACCTGATTTGAATTGGGAGAATGAAGACTGTAGAAAGGCAATCTACGAAAGTGCC GTTGGATACTGGTTAGACCATGGTGTAGACGGCTTTAGAATTGATGTCGGAAGTTTGTAC TCCAAAGTTGTAGGTTTACCAGATGCTCCTGTTGTTGACAAAAACTCGACTTGGCAATCC AGTGATCCATACACATTGAATGGACCACGTATTCACGAGTTCCATCAAGAAATGAATCAA TTCATCAGAAACAGAGTGAAGGATGGCAGGGAGATTATGACAGTTGGTGAAATGCAACAT GCCTCCGACGAAACTAAGAGACTTTATACGAGTGCTTCAAGACACGAACTTAGTGAGTTA TTTAACTTTTCCCACACTGATGTGGGGACTTCACCTTTGTTCCGTTACAACTTGGTCCCA TTTGAACTGAAGGATTGGAAGATTGCCCTTGCTGAGCTGTTCAGGTACATTAATGGTACA GATTGTTGGTCAACAATCTATCTGGAAAATCACGACCAACCTCGTTCAATTACGAGATTT GGTGACGATTCTCCCAAGAACCGTGTTATTTCTGGTAAGTTACTCTCTGTGTTGCTAAGT GCCTTGACCGGTACTCTATATGTGTATCAGGGACAAGAGCTTGGCCAAATCAATTTCAAG AACTGGCCTGTTGAAAAGTACGAGGATGTCGAAATCAGAAACAACTACAATGCCATTAAA GAAGAGCATGGGGAAAACTCAGATGAGATGAAAAAGTTTTTAGAAGCCATTGCCCTTATC TCCAGGGACCATGCTAGAACACCTATGCAATGGTCTCGTGAGGAGCCAAATGCTGGTTTT TCTGGTCCTAGTGCTAAACCATGGTTTTACTTGAACGACTCTTTCAGAGAAGGCATTAAC GTCGAAGATGAAATCAAGGATCCCAACTCGGTTTTGAACTTCTGGAAGGAGGCCTTGAAG TTTAGAAAGGCGCATAAAGACATTACTGTGTACGGATACGATTTCGAGTTTATTGATTTA GACAATAAGAAGTTGTTTAGCTTCACAAAGAAGTACAACAATAAAACATTGTTTGCGGCT TTGAACTTTAGCTCTGATGCGACAGATTTCAAGATTCCAAATGATGATTCATCGTTCAAG TTAGAGTTTGGAAACTATCCAAAGAAGGAGGTAGATGCCTCTTCCAGAACATTGAAGCCA TGGGAAGGAAGAATATATATCAGCGAATGA
Protein Properties
Pfam Domain Function
Protein Residues589
Protein Molecular Weight68591.0
Protein Theoretical pI5.13
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Oligo-1,6-glucosidase MTISSAHPETEPKWWKEATFYQIYPASFKDSNDDGWGDMKGIASKLEYIKELGADAIWIS PFYDSPQDDMGYDIANYEKVWPTYGTNEDCFALIEKTHKLGMKFITDLVINHCSSEHEWF KESRSSKTNPKRDWFFWRPPKGYDAEGKPIPPNNWKSYFGGSAWTFDEKTQEFYLRLFCS TQPDLNWENEDCRKAIYESAVGYWLDHGVDGFRIDVGSLYSKVVGLPDAPVVDKNSTWQS SDPYTLNGPRIHEFHQEMNQFIRNRVKDGREIMTVGEMQHASDETKRLYTSASRHELSEL FNFSHTDVGTSPLFRYNLVPFELKDWKIALAELFRYINGTDCWSTIYLENHDQPRSITRF GDDSPKNRVISGKLLSVLLSALTGTLYVYQGQELGQINFKNWPVEKYEDVEIRNNYNAIK EEHGENSEEMKKFLEAIALISRDHARTPMQWSREEPNAGFSGPSAKPWFYLNDSFREGIN VEDEIKDPNSVLNFWKEALKFRKAHKDITVYGYDFEFIDLDNKKLFSFTKKYNNKTLFAA LNFSSDATDFKIPNDDSSFKLEFGNYPKKEVDASSRTLKPWEGRIYISE
References
External Links
ResourceLink
Saccharomyces Genome Database FSP2
Uniprot IDP53051
Uniprot NameMALX3_YEAST
GenBank Gene IDAB109221
Genebank Protein ID37999034
General Reference
  • Yamamoto, K., Nakayama, A., Yamamoto, Y., Tabata, S. (2004). "Val216 decides the substrate specificity of alpha-glucosidase in Saccharomyces cerevisiae." Eur J Biochem 271:3414-3420.15291818
  • Volckaert, G., Voet, M., Robben, J. (1997). "Sequence analysis of a near-subtelomeric 35.4 kb DNA segment on the right arm of chromosome VII from Saccharomyces cerevisiae carrying the MAL1 locus reveals 15 complete open reading frames, including ZUO1, BGL2 and BIO2 genes and an ABC transporter gene." Yeast 13:251-259.9090054
  • Tettelin, H., Agostoni Carbone, M. L., Albermann, K., Albers, M., Arroyo, J., Backes, U., Barreiros, T., Bertani, I., Bjourson, A. J., Bruckner, M., Bruschi, C. V., Carignani, G., Castagnoli, L., Cerdan, E., Clemente, M. L., Coblenz, A., Coglievina, M., Coissac, E., Defoor, E., Del Bino, S., Delius, H., Delneri, D., de Wergifosse, P., Dujon, B., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Nature 387:81-84.9169869
  • Sickmann, A., Reinders, J., Wagner, Y., Joppich, C., Zahedi, R., Meyer, H. E., Schonfisch, B., Perschil, I., Chacinska, A., Guiard, B., Rehling, P., Pfanner, N., Meisinger, C. (2003). "The proteome of Saccharomyces cerevisiae mitochondria." Proc Natl Acad Sci U S A 100:13207-13212.14576278
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106