Inositol phosphosphingolipids phospholipase C (P40015)

Identification
NameInositol phosphosphingolipids phospholipase C
Synonyms
  • IPS phospholipase C
  • IPS-PLC
  • Neutral sphingomyelinase
  • N-SMase
  • nSMase
Gene NameISC1
Enzyme Class
Biological Properties
General FunctionInvolved in metal ion binding
Specific FunctionResponsible for the hydrolysis of the phosphosphingolipids (IPS), inositol phosphorylceramide (IPC), mannosylinositol phosphorylceramide (MIPC), and mannosyldiinositol phosphorylceramide (M(IP)2C). Also active on sphingomyelin, but this activity is probably not physiologically relevant
Cellular LocationMembrane; Multi-pass membrane protein
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG Reactions
water + inositol phosphoceramide ↔ 1D-myo-inositol 3-phosphate + ceramides
Metabolites
YMDB IDNameView
YMDB007151D-myo-inositol 3-phosphateShow
YMDB00890waterShow
GO ClassificationNot Available
Gene Properties
Chromosome Locationchromosome 5
LocusYER019W
Gene Sequence>1434 bp ATGTACAACAGAAAAGACAGAGATGTTCACGAGAGGAAGGAAGATGGTCAATCTGAGTTT GAAGCACTGAACGGGACCAACGCAATTATGTCCGATAATAGTAAAGCGTATTCCATAAAG TTTCTGACCTTCAATACATGGGGGTTAAAATACGTCTCCAAACACCGTAAAGAAAGACTC AGAGCAATTGCTGATAAATTGGCGGGCCACTCAATGCTTACGCCAATATCTGACGAGTTG TTGCCCAATGGTGGAGATAGTAATGAAAACGAAGATTACGACGTGATTGCCTTACAAGAA ATCTGGTGTGTGGAAGACTGGAAGTATCTAGCTTCTGCGTGTGCCTCCAAGTATCCGTAT CAGCGTTTGTTCCATTCTGGTATTCTGACGGGGCCTGGGTTGGCCATACTGTCCAAGGTC CCGATAGAGTCGACCTTTCTTTACCGGTTCCCGATAAACGGTAGACCGAGTGCGGTGTTC CGTGGCGACTGGTACGTAGGGAAATCTATAGCAATCACCGTATTGAACACAGGAACCCGC CCCATTGCAATAATGAACAGTCACATGCACGCCCCATACGCCAAGCAGGGTGATGCCGCC TACTTGTGCCACAGATCTTGTCAGGCCTGGGATTTCAGCAGGCTCATTAAGCTTTACAGG CAGGCCGGTTATGCGGTGATTGTGGTGGGTGACTTAAACTCCAGACCGGGCTCACTGCCC CACAAATTTCTCACGCAGGAGGCCGGCCTGGTCGACTCCTGGGAGCAATTGCATGGGAAG CAAGACTTGGCGGTGATCGCTCGTCTGTCTCCATTGCAACAATTGCTTAAGGGCTGTACC ACGTGCGATTCGCTGCTCAACACATGGAGGGCCCAAAGACAACCCGATGAGGCATGCAGG TTGGATTATGCTCTTATCGACCCTGATTTCTTGCAAACAGTAGACGCAGGTGTCAGGTTC ACTGAACGGATCCCTCACCTGGACTGCAGTGTCTCTGACCATTTTGCATACTCATGCACC CTTAACATCGTCCCACAGGGCACAGAGTCCCGTCCATCCACCTCCGTTAAGCGTGCGAAG ACTCATGATAGAGAGCTGATCTTGCAGAGATACTCCAACTACGAAACCATGATAGAATGC ATCCACACGTACTTGAAGACAGCCCAAAGACAGAAATTTTTCCGTGGCCTACATTTCTGG GCCTCAATACTTCTCCTAATAGCGTCGTTGGTCGTGACAACGTTTACTGCAAACAAGGCA GGCTGGTCCTCCATCTTCTGGGTCCTTTTCGCTATTGCTGTCTCCATCTCGGGCACCATC GACGGTGCCATCTCCTTCTTGTTTGGCAGGTCTGAAATCAGAGCCCTCATCGAAGTCGAA CAAGAGGTTCTGGACGCGGAGCACCACCTGCAAACTTTCTTGAGCGAGAAATGA
Protein Properties
Pfam Domain Function
Protein Residues477
Protein Molecular Weight53939.89844
Protein Theoretical pI7.58
Signalling Regions
  • None
Transmembrane Regions
  • 399-417
  • 425-449
Protein Sequence>Inositol phosphosphingolipids phospholipase C MYNRKDRDVHERKEDGQSEFEALNGTNAIMSDNSKAYSIKFLTFNTWGLKYVSKHRKERL RAIADKLAGHSMLTPISDELLPNGGDSNENEDYDVIALQEIWCVEDWKYLASACASKYPY QRLFHSGILTGPGLAILSKVPIESTFLYRFPINGRPSAVFRGDWYVGKSIAITVLNTGTR PIAIMNSHMHAPYAKQGDAAYLCHRSCQAWDFSRLIKLYRQAGYAVIVVGDLNSRPGSLP HKFLTQEAGLVDSWEQLHGKQDLAVIARLSPLQQLLKGCTTCDSLLNTWRAQRQPDEACR LDYALIDPDFLQTVDAGVRFTERIPHLDCSVSDHFAYSCTLNIVPQGTESRPSTSVKRAK THDRELILQRYSNYETMIECIHTYLKTAQRQKFFRGLHFWASILLLIASLVVTTFTANKA GWSSIFWVLFAIAVSISGTIDGAISFLFGRSEIRALIEVEQEVLDAEHHLQTFLSEK
References
External Links
ResourceLink
Saccharomyces Genome Database ISC1
Uniprot IDP40015
Uniprot NameISC1_YEAST
GenBank Gene IDU18778
Genebank Protein ID603611
General Reference
  • Dietrich, F. S., Mulligan, J., Hennessy, K., Yelton, M. A., Allen, E., Araujo, R., Aviles, E., Berno, A., Brennan, T., Carpenter, J., Chen, E., Cherry, J. M., Chung, E., Duncan, M., Guzman, E., Hartzell, G., Hunicke-Smith, S., Hyman, R. W., Kayser, A., Komp, C., Lashkari, D., Lew, H., Lin, D., Mosedale, D., Davis, R. W., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Nature 387:78-81.9169868
  • Sawai, H., Okamoto, Y., Luberto, C., Mao, C., Bielawska, A., Domae, N., Hannun, Y. A. (2000). "Identification of ISC1 (YER019w) as inositol phosphosphingolipid phospholipase C in Saccharomyces cerevisiae." J Biol Chem 275:39793-39798.11006294
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258