Dolichyl-phosphate-mannose--protein mannosyltransferase 1 (P33775)

Identification
NameDolichyl-phosphate-mannose--protein mannosyltransferase 1
SynonymsNot Available
Gene NamePMT1
Enzyme Class
Biological Properties
General FunctionInvolved in mannosyltransferase activity
Specific FunctionTransfers mannose from Dol-P-mannose to Ser or Thr residues on proteins. Seems to be active on chitinase
Cellular LocationEndoplasmic reticulum membrane; Multi-pass membrane protein
SMPDB PathwaysNot Available
KEGG Pathways
O-Mannosyl glycan biosynthesisec00514 Map00514
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00937dolichyl D-mannosyl phosphateShow
GO Classification
Component
cell part
membrane
Function
catalytic activity
transferase activity
transferase activity, transferring glycosyl groups
transferase activity, transferring hexosyl groups
mannosyltransferase activity
Process
protein amino acid O-linked glycosylation
metabolic process
macromolecule metabolic process
macromolecule modification
protein modification process
protein amino acid glycosylation
Gene Properties
Chromosome Locationchromosome 4
LocusYDL095W
Gene Sequence>2454 bp ATGTCGGAAGAGAAAACGTACAAACGTGTAGAGCAGGATGATCCCGTGCCCGAACTGGAT ATCAAGCAGGGCCCCGTAAGACCCTTTATTGTTACCGATCCGAGTGCCGAATTGGCCTCG TTACGAACCATGGTCACTCTTAAAGAGAAGCTGTTAGTGGCCTGTCTTGCTGTCTTTACA GCGGTCATTAGATTGCATGGCTTGGCATGGCCTGACAGCGTGGTGTTTGATGAAGTACAT TTCGGTGGGTTTGCCTCGCAATACATTAGGGGGACTTACTTCATGGATGTGCATCCTCCT CTTGCAAAGATGTTGTATGCTGGTGTGGCATCGCTTGGTGGGTTCCAGGGTGATTTTGAC TTCGAAAATATTGGTGACAGCTTTCCATCTACGACGCCATACGTGTTGATGAGATTTTTC TCTGCTTCTTTGGGGGCTCTTACTGTTATTTTGATGTACATGACTTTACGTTATTCTGGT GTTCGTATGTGGGTTGCTTTGATGAGCGCTATCTGCTTTGCCGTTGAAAACTCGTACGTC ACTATTTCTCGTTACATTCTGTTGGACGCCCCATTGATGTTTTTCATTGCAGCTGCAGTC TACTCTTTCAAGAAATACGAAATGTACCCTGCCAACTCGCTCAATGCTTACAAGTCCTTG CTTGCTACTGGTATTGCTCTTGGTATGGCATCTTCATCCAAATGGGTTGGTCTTTTCACG GTTACATGGGTGGGTCTTTTATGTATCTGGAGACTATGGTTCATGATTGGGGATTTGACT AAGTCTTCCAAGTCCATCTTCAAAGTAGCATTTGCCAAATTGGCCTTCTTGTTGGGTGTG CCTTTTGCCCTTTATCTGGTCTTCTTTTATATCCACTTCCAATCATTAACTTTGGACGGG GATGGCGCAAGCTTCTTTTCGCCTGAATTTAGATCTACACTAAAGAACAATAAGATCCCC CAAAATGTCGTTGCTGATGTCGGCATTGGCTCCATTATCAGCTTGCGTCATCTCTCTACC ATGGGCGGTTATTTGCATTCTCATTCACACAATTATCCAGCTGGTTCGGAACAACAACAA AGCACTTTATATCCTCACATGGATGCCAATAACGATTGGTTGTTGGAACTTTACAACGCA CCCGGCGAATCTTTAACAACATTCCAAAACCTAACCGATGGTACCAAGGTCAGACTATTC CACACTGTTACAAGATGTAGATTACACTCTCATGACCATAAGCCACCCGTTTCAGAAAGC AGCGACTGGCAGAAGGAGGTTTCTTGTTATGGTTACAGCGGATTCGACGGTGATGCTAAT GATGACTGGGTTGTTGAGATTGATAAAAAGAATTCTGCTCCTGGAGTTGCCCAAGAACGG GTCATAGCTTTGGACACAAAGTTTAGATTGAGACATGCTATGACAGGCTGTTATTTGTTT TCCCACGAAGTCAAGTTGCCAGCTTGGGGGTTCGAACAACAAGAAGTTACCTGTGCCTCC TCCGGTAGACATGATTTAACATTGTGGTACGTTGAGAACAACAGTAACCCATTGTTACCA GAAGATACCAAGCGTATTTCCTATAAACCTGCAAGCTTCATTTCTAAATTTATTGAATCC CATAAAAAGATGTGGCATATCAATAAAAATTTGGTCGAACCTCATGTTTATGAATCACAA CCAACTTCATGGCCATTCTTGCTACGTGGTATAAGTTACTGGGGTGAAAATAACAGAAAC GTCTATCTATTAGGTAATGCGATCGTATGGTGGGCTGTCACCGCTTTCATCGGTATTTTC GGATTGATTGTTATCACTGAGCTGTTCTCGTGGCAGTTAGGTAAACCAATTTTGAAGGAC TCCAAGGTTGTTAACTTCCACGTTCAGGTTATTCACTACTTATTGGGTTTTGCCGTCCAT TATGCTCCATCTTTCTTAATGCAACGTCAAATGTTTTTGCATCACTACTTACCTGCTTAT TATTTCGGTATTCTTGCCCTTGGCCACGCCTTGGACATAATAGTTTCTTATGTTTTCCGC AGCAAGAGACAAATGGGCTACGCGGTAGTGATCACTTTCCTTGCTGCTTCTGTGTATTTC TTCAAGAGCTTCAGTCCAATTATTTACGGTACACCATGGACTCAAGAATTGTGTCAAAAA TCGCAGTGGTTGTCTGGTTGGGACTACAATTGTAACACATACTTTTCTTCATTAGAAGAG TACAAAAACCAAACCTTGACTAAACGTGAATCTCAACCTGCCGCCACTAGTACAGTTGAA GAAATCACTATAGAAGGGGACGGTCCGTCGTATGAAGATCTCATGAACGAGGATGGCAAG AAAATCTTTAAAGACACAGAAGGTAATGAACTAGATCCAGAAGTTGTCAAAAAAATGTTG GAAGAGGAGGGAGCTAACATTTTAAAAGTAGAAAAAAGGGCTGTTTTGGAATAA
Protein Properties
Pfam Domain Function
Protein Residues817
Protein Molecular Weight92674.70313
Protein Theoretical pI6.68
Signalling Regions
  • None
Transmembrane Regions
  • 51-71
  • 134-154
  • 180-200
  • 235-259
  • 274-291
  • 585-605
  • 686-710
Protein Sequence>Dolichyl-phosphate-mannose--protein mannosyltransferase 1 MSEEKTYKRVEQDDPVPELDIKQGPVRPFIVTDPSAELASLRTMVTLKEKLLVACLAVFT AVIRLHGLAWPDSVVFDEVHFGGFASQYIRGTYFMDVHPPLAKMLYAGVASLGGFQGDFD FENIGDSFPSTTPYVLMRFFSASLGALTVILMYMTLRYSGVRMWVALMSAICFAVENSYV TISRYILLDAPLMFFIAAAVYSFKKYEMYPANSLNAYKSLLATGIALGMASSSKWVGLFT VTWVGLLCIWRLWFMIGDLTKSSKSIFKVAFAKLAFLLGVPFALYLVFFYIHFQSLTLDG DGASFFSPEFRSTLKNNKIPQNVVADVGIGSIISLRHLSTMGGYLHSHSHNYPAGSEQQQ STLYPHMDANNDWLLELYNAPGESLTTFQNLTDGTKVRLFHTVTRCRLHSHDHKPPVSES SDWQKEVSCYGYSGFDGDANDDWVVEIDKKNSAPGVAQERVIALDTKFRLRHAMTGCYLF SHEVKLPAWGFEQQEVTCASSGRHDLTLWYVENNSNPLLPEDTKRISYKPASFISKFIES HKKMWHINKNLVEPHVYESQPTSWPFLLRGISYWGENNRNVYLLGNAIVWWAVTAFIGIF GLIVITELFSWQLGKPILKDSKVVNFHVQVIHYLLGFAVHYAPSFLMQRQMFLHHYLPAY YFGILALGHALDIIVSYVFRSKRQMGYAVVITFLAASVYFFKSFSPIIYGTPWTQELCQK SQWLSGWDYNCNTYFSSLEEYKNQTLTKRESQPAATSTVEEITIEGDGPSYEDLMNEDGK KIFKDTEGNELDPEVVKKMLEEEGANILKVEKRAVLE
References
External Links
ResourceLink
Saccharomyces Genome Database PMT1
Uniprot IDP33775
Uniprot NamePMT1_YEAST
GenBank Gene IDL19169
Genebank Protein ID388211
General Reference
  • Strahl-Bolsinger, S., Immervoll, T., Deutzmann, R., Tanner, W. (1993). "PMT1, the gene for a key enzyme of protein O-glycosylation in Saccharomyces cerevisiae." Proc Natl Acad Sci U S A 90:8164-8168.8367478
  • Boskovic, J., Soler-Mira, A., Garcia-Cantalejo, J. M., Ballesta, J. P., Jimenez, A., Remacha, M. (1996). "The sequence of a 16,691 bp segment of Saccharomyces cerevisiae chromosome IV identifies the DUN1, PMT1, PMT5, SRP14 and DPR1 genes, and five new open reading frames." Yeast 12:1377-1384.8923743
  • Jacq, C., Alt-Morbe, J., Andre, B., Arnold, W., Bahr, A., Ballesta, J. P., Bargues, M., Baron, L., Becker, A., Biteau, N., Blocker, H., Blugeon, C., Boskovic, J., Brandt, P., Bruckner, M., Buitrago, M. J., Coster, F., Delaveau, T., del Rey, F., Dujon, B., Eide, L. G., Garcia-Cantalejo, J. M., Goffeau, A., Gomez-Peris, A., Zaccaria, P., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Nature 387:75-78.9169867
  • Gentzsch, M., Tanner, W. (1996). "The PMT gene family: protein O-glycosylation in Saccharomyces cerevisiae is vital." EMBO J 15:5752-5759.8918452
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258