Dolichyl-phosphate-mannose--protein mannosyltransferase 1 (P33775)

Identification
NameDolichyl-phosphate-mannose--protein mannosyltransferase 1
SynonymsNot Available
Gene NamePMT1
Enzyme Class
Biological Properties
General FunctionInvolved in mannosyltransferase activity
Specific FunctionTransfers mannose from Dol-P-mannose to Ser or Thr residues on proteins. Seems to be active on chitinase
Cellular LocationEndoplasmic reticulum membrane; Multi-pass membrane protein
SMPDB PathwaysNot Available
KEGG Pathways
O-Mannosyl glycan biosynthesisec00514 Map00514
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00937dolichyl D-mannosyl phosphateShow
GO Classification
Component
cell part
membrane
Function
catalytic activity
transferase activity
transferase activity, transferring glycosyl groups
transferase activity, transferring hexosyl groups
mannosyltransferase activity
Process
metabolic process
macromolecule metabolic process
macromolecule modification
protein modification process
protein amino acid glycosylation
protein amino acid O-linked glycosylation
Gene Properties
Chromosome Locationchromosome 4
LocusYDL095W
Gene Sequence>2454 bp ATGTCGGAAGAGAAAACGTACAAACGTGTAGAGCAGGATGATCCCGTGCCCGAACTGGAT ATCAAGCAGGGCCCCGTAAGACCCTTTATTGTTACCGATCCGAGTGCCGAATTGGCCTCG TTACGAACCATGGTCACTCTTAAAGAGAAGCTGTTAGTGGCCTGTCTTGCTGTCTTTACA GCGGTCATTAGATTGCATGGCTTGGCATGGCCTGACAGCGTGGTGTTTGATGAAGTACAT TTCGGTGGGTTTGCCTCGCAATACATTAGGGGGACTTACTTCATGGATGTGCATCCTCCT CTTGCAAAGATGTTGTATGCTGGTGTGGCATCGCTTGGTGGGTTCCAGGGTGATTTTGAC TTCGAAAATATTGGTGACAGCTTTCCATCTACGACGCCATACGTGTTGATGAGATTTTTC TCTGCTTCTTTGGGGGCTCTTACTGTTATTTTGATGTACATGACTTTACGTTATTCTGGT GTTCGTATGTGGGTTGCTTTGATGAGCGCTATCTGCTTTGCCGTTGAAAACTCGTACGTC ACTATTTCTCGTTACATTCTGTTGGACGCCCCATTGATGTTTTTCATTGCAGCTGCAGTC TACTCTTTCAAGAAATACGAAATGTACCCTGCCAACTCGCTCAATGCTTACAAGTCCTTG CTTGCTACTGGTATTGCTCTTGGTATGGCATCTTCATCCAAATGGGTTGGTCTTTTCACG GTTACATGGGTGGGTCTTTTATGTATCTGGAGACTATGGTTCATGATTGGGGATTTGACT AAGTCTTCCAAGTCCATCTTCAAAGTAGCATTTGCCAAATTGGCCTTCTTGTTGGGTGTG CCTTTTGCCCTTTATCTGGTCTTCTTTTATATCCACTTCCAATCATTAACTTTGGACGGG GATGGCGCAAGCTTCTTTTCGCCTGAATTTAGATCTACACTAAAGAACAATAAGATCCCC CAAAATGTCGTTGCTGATGTCGGCATTGGCTCCATTATCAGCTTGCGTCATCTCTCTACC ATGGGCGGTTATTTGCATTCTCATTCACACAATTATCCAGCTGGTTCGGAACAACAACAA AGCACTTTATATCCTCACATGGATGCCAATAACGATTGGTTGTTGGAACTTTACAACGCA CCCGGCGAATCTTTAACAACATTCCAAAACCTAACCGATGGTACCAAGGTCAGACTATTC CACACTGTTACAAGATGTAGATTACACTCTCATGACCATAAGCCACCCGTTTCAGAAAGC AGCGACTGGCAGAAGGAGGTTTCTTGTTATGGTTACAGCGGATTCGACGGTGATGCTAAT GATGACTGGGTTGTTGAGATTGATAAAAAGAATTCTGCTCCTGGAGTTGCCCAAGAACGG GTCATAGCTTTGGACACAAAGTTTAGATTGAGACATGCTATGACAGGCTGTTATTTGTTT TCCCACGAAGTCAAGTTGCCAGCTTGGGGGTTCGAACAACAAGAAGTTACCTGTGCCTCC TCCGGTAGACATGATTTAACATTGTGGTACGTTGAGAACAACAGTAACCCATTGTTACCA GAAGATACCAAGCGTATTTCCTATAAACCTGCAAGCTTCATTTCTAAATTTATTGAATCC CATAAAAAGATGTGGCATATCAATAAAAATTTGGTCGAACCTCATGTTTATGAATCACAA CCAACTTCATGGCCATTCTTGCTACGTGGTATAAGTTACTGGGGTGAAAATAACAGAAAC GTCTATCTATTAGGTAATGCGATCGTATGGTGGGCTGTCACCGCTTTCATCGGTATTTTC GGATTGATTGTTATCACTGAGCTGTTCTCGTGGCAGTTAGGTAAACCAATTTTGAAGGAC TCCAAGGTTGTTAACTTCCACGTTCAGGTTATTCACTACTTATTGGGTTTTGCCGTCCAT TATGCTCCATCTTTCTTAATGCAACGTCAAATGTTTTTGCATCACTACTTACCTGCTTAT TATTTCGGTATTCTTGCCCTTGGCCACGCCTTGGACATAATAGTTTCTTATGTTTTCCGC AGCAAGAGACAAATGGGCTACGCGGTAGTGATCACTTTCCTTGCTGCTTCTGTGTATTTC TTCAAGAGCTTCAGTCCAATTATTTACGGTACACCATGGACTCAAGAATTGTGTCAAAAA TCGCAGTGGTTGTCTGGTTGGGACTACAATTGTAACACATACTTTTCTTCATTAGAAGAG TACAAAAACCAAACCTTGACTAAACGTGAATCTCAACCTGCCGCCACTAGTACAGTTGAA GAAATCACTATAGAAGGGGACGGTCCGTCGTATGAAGATCTCATGAACGAGGATGGCAAG AAAATCTTTAAAGACACAGAAGGTAATGAACTAGATCCAGAAGTTGTCAAAAAAATGTTG GAAGAGGAGGGAGCTAACATTTTAAAAGTAGAAAAAAGGGCTGTTTTGGAATAA
Protein Properties
Pfam Domain Function
Protein Residues817
Protein Molecular Weight92674.70313
Protein Theoretical pI6.68
Signalling Regions
  • None
Transmembrane Regions
  • 51-71
  • 134-154
  • 180-200
  • 235-259
  • 274-291
  • 585-605
  • 686-710
Protein Sequence>Dolichyl-phosphate-mannose--protein mannosyltransferase 1 MSEEKTYKRVEQDDPVPELDIKQGPVRPFIVTDPSAELASLRTMVTLKEKLLVACLAVFT AVIRLHGLAWPDSVVFDEVHFGGFASQYIRGTYFMDVHPPLAKMLYAGVASLGGFQGDFD FENIGDSFPSTTPYVLMRFFSASLGALTVILMYMTLRYSGVRMWVALMSAICFAVENSYV TISRYILLDAPLMFFIAAAVYSFKKYEMYPANSLNAYKSLLATGIALGMASSSKWVGLFT VTWVGLLCIWRLWFMIGDLTKSSKSIFKVAFAKLAFLLGVPFALYLVFFYIHFQSLTLDG DGASFFSPEFRSTLKNNKIPQNVVADVGIGSIISLRHLSTMGGYLHSHSHNYPAGSEQQQ STLYPHMDANNDWLLELYNAPGESLTTFQNLTDGTKVRLFHTVTRCRLHSHDHKPPVSES SDWQKEVSCYGYSGFDGDANDDWVVEIDKKNSAPGVAQERVIALDTKFRLRHAMTGCYLF SHEVKLPAWGFEQQEVTCASSGRHDLTLWYVENNSNPLLPEDTKRISYKPASFISKFIES HKKMWHINKNLVEPHVYESQPTSWPFLLRGISYWGENNRNVYLLGNAIVWWAVTAFIGIF GLIVITELFSWQLGKPILKDSKVVNFHVQVIHYLLGFAVHYAPSFLMQRQMFLHHYLPAY YFGILALGHALDIIVSYVFRSKRQMGYAVVITFLAASVYFFKSFSPIIYGTPWTQELCQK SQWLSGWDYNCNTYFSSLEEYKNQTLTKRESQPAATSTVEEITIEGDGPSYEDLMNEDGK KIFKDTEGNELDPEVVKKMLEEEGANILKVEKRAVLE
References
External Links
ResourceLink
Saccharomyces Genome Database PMT1
Uniprot IDP33775
Uniprot NamePMT1_YEAST
GenBank Gene IDL19169
Genebank Protein ID388211
General Reference
  • Strahl-Bolsinger, S., Immervoll, T., Deutzmann, R., Tanner, W. (1993). "PMT1, the gene for a key enzyme of protein O-glycosylation in Saccharomyces cerevisiae." Proc Natl Acad Sci U S A 90:8164-8168.8367478
  • Boskovic, J., Soler-Mira, A., Garcia-Cantalejo, J. M., Ballesta, J. P., Jimenez, A., Remacha, M. (1996). "The sequence of a 16,691 bp segment of Saccharomyces cerevisiae chromosome IV identifies the DUN1, PMT1, PMT5, SRP14 and DPR1 genes, and five new open reading frames." Yeast 12:1377-1384.8923743
  • Jacq, C., Alt-Morbe, J., Andre, B., Arnold, W., Bahr, A., Ballesta, J. P., Bargues, M., Baron, L., Becker, A., Biteau, N., Blocker, H., Blugeon, C., Boskovic, J., Brandt, P., Bruckner, M., Buitrago, M. J., Coster, F., Delaveau, T., del Rey, F., Dujon, B., Eide, L. G., Garcia-Cantalejo, J. M., Goffeau, A., Gomez-Peris, A., Zaccaria, P., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Nature 387:75-78.9169867
  • Gentzsch, M., Tanner, W. (1996). "The PMT gene family: protein O-glycosylation in Saccharomyces cerevisiae is vital." EMBO J 15:5752-5759.8918452
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258