Identification
NameAlkylphosphocholine resistance protein LEM3
Synonyms
  • Brefeldin-A sensitivity protein 3
  • Ro-sensitive 3
Gene NameLEM3
Enzyme ClassNot Available
Biological Properties
General FunctionInvolved in protein binding
Specific FunctionInvolved in the asymmetrical organization of phosphatidylethanolamine in the plasma membrane
Cellular LocationCell membrane; Multi-pass membrane protein
SMPDB Pathways
Lysolipid incorporation into ERPW002532 ThumbThumb?image type=greyscaleThumb?image type=simple
Lysolipid incorporation into ER PC(10:0/10:0)PW002780 ThumbThumb?image type=greyscaleThumb?image type=simple
Lysolipid incorporation into ER PC(14:0/14:0)PW002783 ThumbThumb?image type=greyscaleThumb?image type=simple
Lysolipid incorporation into ER PC(16:0/16:0)PW002784 ThumbThumb?image type=greyscaleThumb?image type=simple
Lysolipid incorporation into ER PC(16:1(11Z)/16:1(11Z))PW002786 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG PathwaysNot Available
SMPDB Reactions
Adenosine triphosphate + water + PC(14:0/0:0)ADP + phosphate + PC(14:0/0:0)
Adenosine triphosphate + water + PE(14:0/0:0)ADP + phosphate + PE(14:0/0:0)
Adenosine triphosphate + water + Lyso-PS(14:0/0:0) → ADP + phosphate + Lyso-PS(14:0/0:0)
Lyso-PI(14:0/0:0) + Adenosine triphosphate + water → Lyso-PI(14:0/0:0) + ADP + phosphate
Lyso-PS(0:0/14:0) + Adenosine triphosphate + waterLyso-PS(0:0/14:0) + ADP + phosphate
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00109Adenosine triphosphateShow
YMDB00890waterShow
YMDB00907phosphateShow
YMDB00914ADPShow
YMDB01175PC(14:0/0:0)Show
YMDB01194PE(14:0/0:0)Show
YMDB13347Lyso-PI(0:0/14:0)Show
YMDB13365Lyso-PS(0:0/14:0)Show
GO Classification
Component
cell part
membrane
Gene Properties
Chromosome LocationNot Available
Locus
Gene Sequence>1245 bp ATGGTAAATTTCGATTTGGGCCAAGTTGGTGAAGTATTCCGTAGAAAGGACAAAGGAGCT ATAGTGTCCGGAGACAACCCAGAAGAAGAAGAAGACGTCGATGCTTCTGAGTTTGAAGAG GATGAAGTAAAGCCTGTGCGTACAAAGAACAGAAGACCCAAAGAGGATGCATTCACTCAA CAAAGATTAGCGGCTATAAACCCGGTGTTAACGCCGAGGACAGTTTTGCCTCTTTATCTG TTAATTGCTGTTGTATTTGTTATTGTAGGAGGGTGCATATTAGCACAGAACTCCAAAGTG GATGAAGTAACCATATATTACCAGGACTGTATGACTAACGCAACCTCATCATGGAGCGAC ATCCCTTCTGAACATTGGCAATTTGTATTTCACAAATATAAAACATATAATACTGCACCA CAATGGAGGTTTGTGGATGATGAATCAGATGACTTCACAAAACAAAGAGGTACATGCCAA ATCAGGTTCACTACTCCAAGTGATATGAAAAATAACGTATATTTGAATTATGTGCTGGAA AAATTTGCTGCCAATCACAGAAGATATGTACTATCTTTTAGCGAAGATCAGATACGTGGG GAGGACGCCTCTTATGAGACGGTGCATGATGCAACCGGTATAAATTGTAAGCCACTATCT AAGAACGCCGATGGTAAAATATACTATCCATGTGGCCTAATTGCGAACTCAATGTTCAAC GACACGTTCCCCCTGCAGTTAACAAATGTAGGGGATACGTCTAACAACTATTCATTGACA AATAAAGGAATCAATTGGGAATCTGATAAAAAAAGGTACAAAAAAACCAAATACAATTAT ACACAGATAGCACCTCCACCATATTGGGAAAAGATGTATCCAGATGGATACAACGAGACG AATATTCCTGATATTCAGGATTGGGAAGAGTTCCAAAACTGGATGAGGCCAGGTGCTTTT GATAAAATTACAAAACTGATCAGAATCAATAAAAACGATACTTTGCCAGCGGGTGAATAC CAGTTGGATATCGGTCTGCATTGGCCAGTATTGGAGTTTAATGGTAAGAAGGGAATCTAC TTGACTCACGGTTCTCACCTTGGCGGTAGAAATCCATTTTTAGGTATAGTTTACCTGATT GGAGGCTGTATTTGTGCTGCCATGGCATTGATATTATTAACGTTTTGGCTCTTCGGCGGT AGAAAGATTGCTGATGCATCAAGTTTGTCATGGAATATGAAATAG
Protein Properties
Pfam Domain Function
Protein Residues414
Protein Molecular Weight47437.19922
Protein Theoretical pI5.72
Signalling Regions
  • None
Transmembrane Regions
  • 75-95
  • 373-393
Protein Sequence>Alkylphosphocholine resistance protein LEM3 MVNFDLGQVGEVFRRKDKGAIVSGDNPEEEEDVDASEFEEDEVKPVRTKNRRPKEDAFTQ QRLAAINPVLTPRTVLPLYLLIAVVFVIVGGCILAQNSKVDEVTIYYQDCMTNATSSWSD IPSEHWQFVFHKYKTYNTAPQWRFVDDESDDFTKQRGTCQIRFTTPSDMKNNVYLNYVLE KFAANHRRYVLSFSEDQIRGEDASYETVHDATGINCKPLSKNADGKIYYPCGLIANSMFN DTFPLQLTNVGDTSNNYSLTNKGINWESDKKRYKKTKYNYTQIAPPPYWEKMYPDGYNET NIPDIQDWEEFQNWMRPGAFDKITKLIRINKNDTLPAGEYQLDIGLHWPVLEFNGKKGIY LTHGSHLGGRNPFLGIVYLIGGCICAAMALILLTFWLFGGRKIADASSLSWNMK
References
External Links
ResourceLink
Saccharomyces Genome Database LEM3
Uniprot IDP42838
Uniprot NameLEM3_YEAST
GenBank Gene IDZ46259
Genebank Protein ID633662
General Reference
  • Maftahi, M., Nicaud, J. M., Levesque, H., Gaillardin, C. (1995). "Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames." Yeast 11:567-572.7645347
  • Philippsen, P., Kleine, K., Pohlmann, R., Dusterhoft, A., Hamberg, K., Hegemann, J. H., Obermaier, B., Urrestarazu, L. A., Aert, R., Albermann, K., Altmann, R., Andre, B., Baladron, V., Ballesta, J. P., Becam, A. M., Beinhauer, J., Boskovic, J., Buitrago, M. J., Bussereau, F., Coster, F., Crouzet, M., D'Angelo, M., Dal Pero, F., De Antoni, A., Del Rey, F., Doignon, F., Domdey, H., Dubois, E., Fiedler, T., Fleig, U., Floeth, M., Fritz, C., Gaillardin, C., Garcia-Cantalejo, J. M., Glansdorff, N. N., Goffeau, A., Gueldener, U., Herbert, C., Heumann, K., Heuss-Neitzel, D., Hilbert, H., Hinni, K., Iraqui Houssaini, I., Jacquet, M., Jimenez, A., Jonniaux, J. L., Karpfinger, L., Lanfranchi, G., Lepingle, A., Levesque, H., Lyck, R., Maftahi, M., Mallet, L., Maurer, K. C., Messenguy, F., Mewes, H. W., Mosti, D., Nasr, F., Nicaud, J. M., Niedenthal, R. K., Pandolfo, D., Pierard, A., Piravandi, E., Planta, R. J., Pohl, T. M., Purnelle, B., Rebischung, C., Remacha, M., Revuelta, J. L., Rinke, M., Saiz, J. E., Sartorello, F., Scherens, B., Sen-Gupta, M., Soler-Mira, A., Urbanus, J. H., Valle, G., Van Dyck, L., Verhasselt, P., Vierendeels, F., Vissers, S., Voet, M., Volckaert, G., Wach, A., Wambutt, R., Wedler, H., Zollner, A., Hani, J. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Nature 387:93-98.9169873
  • Kato, U., Emoto, K., Fredriksson, C., Nakamura, H., Ohta, A., Kobayashi, T., Murakami-Murofushi, K., Kobayashi, T., Umeda, M. (2002). "A novel membrane protein, Ros3p, is required for phospholipid translocation across the plasma membrane in Saccharomyces cerevisiae." J Biol Chem 277:37855-37862.12133835
  • Hanson, P. K., Malone, L., Birchmore, J. L., Nichols, J. W. (2003). "Lem3p is essential for the uptake and potency of alkylphosphocholine drugs, edelfosine and miltefosine." J Biol Chem 278:36041-36050.12842877
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
  • Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956