Alpha-1,2 mannosyltransferase KTR1 (P27810)

Identification
NameAlpha-1,2 mannosyltransferase KTR1
SynonymsNot Available
Gene NameKTR1
Enzyme Class
Biological Properties
General FunctionInvolved in mannosyltransferase activity
Specific FunctionMannosyltransferase that transfers a mannose residue from GDP-mannose to a range of acceptors in vitro
Cellular LocationGolgi apparatus membrane; Single-pass type II membrane protein
SMPDB Pathways
N-Glycan biosynthesisPW002501 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
N-Glycan biosynthesisec00510 Map00510
SMPDB ReactionsNot Available
KEGG Reactions
Beta-1,4-D-Mannosylchitobiosyldiphosphodolichol + Guanosine diphosphate mannose → alpha-D-mannosyl-beta-D-mannosyldiacetylchitobiosyldiphosphodolichol + hydron + Guanosine diphosphate
Metabolites
YMDB IDNameView
YMDB00202Guanosine diphosphate mannoseShow
YMDB00257Guanosine diphosphateShow
YMDB00581alpha-D-mannosyl-beta-D-mannosyldiacetylchitobiosyldiphosphodolicholShow
YMDB00862hydronShow
YMDB00935beta-D-mannosyldiacetylchitobiosyldiphosphodolicholShow
GO Classification
Component
cell part
membrane
Function
alpha-1,2-mannosyltransferase activity
glycolipid 2-alpha-mannosyltransferase activity
catalytic activity
transferase activity
transferase activity, transferring glycosyl groups
transferase activity, transferring hexosyl groups
mannosyltransferase activity
Process
macromolecule metabolic process
macromolecule modification
protein modification process
protein amino acid glycosylation
metabolic process
Gene Properties
Chromosome Locationchromosome 15
LocusYOR099W
Gene Sequence>1182 bp ATGGCGAAGATTATGATCCCAGCTAGCAAGCAGCCTGTTTACAAAAAATTAGGACTTCTT CTGGTCGCCGTGTTTACTGTGTATGTGTTCTTTCATGGAGCTCAGTATGCGAGAGGCTCT GCCCCAAGCCCAAAGTACAGTACCGTGCTATCTTCCGGCTCGGGCTATAAATACTCGAAA GTGGAACTACCAAAGTATACCGGTCCTCGGGAAAAGGCCACTTTTGTCACTCTAGTGCGT AATAGAGATCTATATTCGTTGGCTGAGTCTATTAAATCCGTGGAAGATAGATTTAACTCC AAGTTCAACTATGATTGGGTGTTTTTGAATGATGAAGAGTTCACTGATGAATTCAAAAAC GTTACGTCAGCTTTAGTTAGTGGTACAACAAAATACGGTGTCATCCCAAAGGAACATTGG TCCTTCCCTGAGTGGATTGATGAAGAAAAGGCCGCTCAAGTCAGGAAGGAGATGGGTGAG AAGAGAATCATCTACGGTGATTCTATCTCTTACAGACACATGTGTCGTTTTGAATCAGGC TTCTTTTACAGACATCCTTTGATGGATGACTACGATTGGTATTGGCGTGTGGAACCTGAT ATCAAGTTGCACTGTGATATCGACTACGATGTCTTCAAATTCATGAAAGACAATAAGAAA AAGTATGCCTTTGCCATTTCCATTAAGGAGTATGAAGCTACTATCCCCACTTTATGGGAG ACTACTCGTAAATTCATGGAAGCCCATCCTGAGCTTATCCATGAAAATAATATGTTAGAC TTTGTTAGCGACGATCAGGGACTTTCATACAATTTGTGCCATTTTTGGTCTAATTTTGAA ATTGCTGCTTTGGATTTGTGGAGATCTCCAGCATACTCCGCATATTTTGATTATTTAGAT CGTGAAGGTGGATTCTTTTATGAAAGATGGGGTGATGCTCCAGTTCATTCTATTGGTGCT GCGCTGTTCTTGGACCGCTCTGAAATCCATCACTTCGGTGATATTGGTTATTACCATGTT CCATTTCACTCCTGTCCAATAGACACAAGTATCAGACTGGCTAACAAATGTGACTGTGAT CCAAGCAAAGATTTCACATGGCACAGTTATTCATGTACTACTAAATTCTACAATATAAAC AAGTTGCCAAAGCCTGCTGGTTGGCAAAACCATATCGGTTGA
Protein Properties
Pfam Domain Function
Protein Residues393
Protein Molecular Weight46021.69922
Protein Theoretical pI6.67
Signalling Regions
  • None
Transmembrane Regions
  • 17-34
Protein Sequence>Alpha-1,2 mannosyltransferase KTR1 MAKIMIPASKQPVYKKLGLLLVAVFTVYVFFHGAQYARGSAPSPKYSTVLSSGSGYKYSK VELPKYTGPREKATFVTLVRNRDLYSLAESIKSVEDRFNSKFNYDWVFLNDEEFTDEFKN VTSALVSGTTKYGVIPKEHWSFPEWIDEEKAAQVRKEMGEKRIIYGDSISYRHMCRFESG FFYRHPLMDDYDWYWRVEPDIKLHCDIDYDVFKFMKDNKKKYAFAISIKEYEATIPTLWE TTRKFMEAHPELIHENNMLDFVSDDQGLSYNLCHFWSNFEIAALDLWRSPAYSAYFDYLD REGGFFYERWGDAPVHSIGAALFLDRSEIHHFGDIGYYHVPFHSCPIDTSIRLANKCDCD PSKDFTWHSYSCTTKFYNINKLPKPAGWQNHIG
References
External Links
ResourceLink
Saccharomyces Genome Database KTR1
Uniprot IDP27810
Uniprot NameKTR1_YEAST
GenBank Gene IDX62941
Genebank Protein ID3866
General Reference
  • Hill, K., Boone, C., Goebl, M., Puccia, R., Sdicu, A. M., Bussey, H. (1992). "Yeast KRE2 defines a new gene family encoding probable secretory proteins, and is required for the correct N-glycosylation of proteins." Genetics 130:273-283.1541391
  • Voss, H., Benes, V., Andrade, M. A., Valencia, A., Rechmann, S., Teodoru, C., Schwager, C., Paces, V., Sander, C., Ansorge, W. (1997). "DNA sequencing and analysis of 130 kb from yeast chromosome XV." Yeast 13:655-672.9200815
  • Dujon, B., Albermann, K., Aldea, M., Alexandraki, D., Ansorge, W., Arino, J., Benes, V., Bohn, C., Bolotin-Fukuhara, M., Bordonne, R., Boyer, J., Camasses, A., Casamayor, A., Casas, C., Cheret, G., Cziepluch, C., Daignan-Fornier, B., Dang, D. V., de Haan, M., Delius, H., Durand, P., Fairhead, C., Feldmann, H., Gaillon, L., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Nature 387:98-102.9169874
  • Romero, P. A., Lussier, M., Sdicu, A. M., Bussey, H., Herscovics, A. (1997). "Ktr1p is an alpha-1,2-mannosyltransferase of Saccharomyces cerevisiae. Comparison of the enzymic properties of soluble recombinant Ktr1p and Kre2p/Mnt1p produced in Pichia pastoris." Biochem J 321 ( Pt 2):289-295.9020857
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Kung, L. A., Tao, S. C., Qian, J., Smith, M. G., Snyder, M., Zhu, H. (2009). "Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes." Mol Syst Biol 5:308.19756047