AdoMet-dependent rRNA methyltransferase SPB1 (P25582)

Identification

Name

AdoMet-dependent rRNA methyltransferase SPB1

Synonyms

2'-O-ribose RNA methyltransferase
S-adenosyl-L-methionine-dependent methyltransferase
Suppressor of PAB1 protein 1

Gene Name

SPB1

Enzyme Class

2.1.1.-

Biological Properties

General Function

Involved in methyltransferase activity

Specific Function

Required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Specifically methylates the guanosine in position 2922 of the 25S rRNA at the stage of 27S pre-rRNA maturation. Methylates also the uridine in position 2921 in the absence of methylation of this residue guided by snoRNA snR52 at the stage of 35S pre-rRNA maturation

Cellular Location

Nucleus, nucleolus

SMPDB Pathways

Not Available

KEGG Pathways

Not Available

SMPDB Reactions

Not Available

KEGG Reactions

Not Available

Metabolites

YMDB ID	Name	View
YMDB00198	S-Adenosylhomocysteine	Show
YMDB00297	S-Adenosylmethionine	Show

GO Classification

Component
organelle
membrane-bounded organelle
intracellular membrane-bounded organelle
nucleus
Function
nucleic acid binding
catalytic activity
transferase activity
transferase activity, transferring one-carbon groups
methyltransferase activity
binding
Process
ncRNA metabolic process
one-carbon metabolic process
methylation
metabolic process
rRNA metabolic process
macromolecule metabolic process
rRNA processing
rRNA modification
rRNA methylation
cellular metabolic process
cellular macromolecule metabolic process
RNA metabolic process

Gene Properties

Chromosome Location

chromosome 3

Locus

YCL054W

Gene Sequence

>2526 bp ATGGGTAAGACACAAAAGAAGAATAGTAAGGGACGTTTAGATAGATACTATTATCTAGCA AAGGAGAAAGGTTATCGTGCTCGTTCATCCTTTAAGATTATTCAGATTAATGAAAAGTAT GGCCACTTCTTAGAAAAATCGAAGGTTGTTATTGATCTGTGTGCTGCTCCTGGTTCATGG TGTCAAGTTGCATCCAAACTCTGTCCTGTCAACTCCTTAATTATTGGTGTTGATATTGTT CCAATGAAGCCGATGCCCAATGTTATAACTTTTCAAAGTGATATTACAACAGAAGATTGT AGATCAAAATTGAGGGGTTATATGAAGACTTGGAAAGCTGATACAGTGTTGCATGATGGT GCTCCTAATGTTGGTTTGGGTTGGGTTCAGGATGCTTTCACCCAATCTCAATTAACCTTA CAAGCTTTGAAGTTGGCCGTCGAAAATTTGGTGGTGAACGGTACTTTTGTTACTAAAATT TTCAGATCCAAGGATTATAATAAATTGATTTGGGTTTTCCAACAATTGTTTGAGAAAGTT GAAGCCACAAAACCACCCGCATCAAGAAATGTTTCTGCAGAAATTTTTGTAGTGTGTAAA GGTTTCAAGGCACCAAAGAGGCTGGACCCAAGATTACTGGATCCAAAAGAAGTTTTTGAA GAATTGCCAGATGGGCAACAGAATATGGAGTCCAAGATTTATAATCCCGAAAAAAAAGTT AGAAAAAGACAAGGTTATGAGGAAGGTGATAATTTACTGTATCATGAAACCTCAATTTTG GATTTTGTCAGGACGGAAGACCCAATAAGCATGCTTGGAGAAATGAATAAGTTTACAATT GATGAGAATGACCATGAATGGAAGATCTTGAAGAAATTGAAACAAACCACAGACGAGTTC CGTTCCTGTATTGAAGATTTAAAAGTCTTGGGTAAAAAGGATTTTAAAATGATCCTAAGA TGGAGAAAGATTGCAAGGGAAATCTTGGGCATCGAAGTTAAGGACGACGCTAAAACAGAA ATTGAAGTAGTACCGTTAACAGAAGAGGAGCAAATTGAAAAAGATTTGCAAGGTTTACAG GAGAAGCAGCGTCTAAATGTCAAGCGCGAAAGAAGAAGGAAGAACGAAATGAAGCAAAAG GAACTACAGAGAATGCAAATGAACATGATAACCCCCACTGATATTGGTATTGAAGCCGCA AGTTTGGGTAAAGAATCGTTGTTTAATTTGAAAACTGCAGAAAAGACTGGTATCTTGAAC GACTTGGCGAAGGGTAAGAAAAGAATGATTTTTACAGACGATGAATTAGCCAAAGATAAT GATATTTACATTGATGAAAACATCATGATCAAAGATAAGGATTCTGCCGCTGATGCGGAC GATTTAGAAAGCGAGTTGAATGCCATGTATAGTGATTATAAAACTAGAAGGTCGGAAAGG GATGCCAAGTTTAGAGCTAAGCAAGCACGTGGTGGCGATAATGAGGAAGAATGGACCGGT TTTAATGAGGGAAGTCTAGAAAAGAAAGAAGAGGAAGGTAAAGATTATATCGAAGACAAT GACGATGAAGGTGTCGAAGGAGACTCCGATGATGATGAAGCCATCACCAATTTGATTAGC AAATTGAAGGGACAAGAAGGTGATCACAAATTGAGTAGTAAGGCACGTATGATTTTCAAT GATCCGATATTTAATAACGTTGAACCTGATTTGCCAGTAAATACCGTCAATGATGGCATA ATGAGTTCCGAGTCTGTTGGTGATATTTCTAAATTAAATAAGAAAAGAAAACATGAAGAA ATGCACCAGAAACAAGATGAAGCAGATTCTTCGGATGAGAGTTCAAGTGATGACTCCGAT TTCGAAATTGTGGCCAACGATAATGCATCAGAAGAATTCGATTCTGATTATGATTCAGAG GAAGAAAAAAATCAAACAAAGAAAGAAAAGCATTCCAGAGACATTGATATTGCTACTGTT GAAGCCATGACTTTGGCACATCAGTTAGCATTGGGTCAGAAAAACAAGCATGATCTTGTT GATGAAGGTTTCAATAGATACACCTTCCGTGACACCGAAAATTTGCCAGATTGGTTTTTA GAAGATGAAAAGGAACATTCAAAGATAAATAAGCCGATTACTAAGGAGGCAGCGATGGCA ATTAAAGAAAAAATAAAGGCAATGAACGCCCGTCCTATTAAGAAGGTTGCTGAAGCTAAG GCAAGAAAGAGGATGCGTGCCGTAGCTCGGTTAGAAAAGATCAAGAAGAAGGCGGGCTTG ATTAACGATGATTCAGACAAGACAGAGAAGGACAAGGCTGAAGAAATTTCTAGATTGATG CGTAAGGTTACCAAAAAACCAAAGACCAAGCCAAAGGTTACTTTGGTTGTTGCCTCAGGT AGGAACAAAGGTTTGGCAGGTAGACCAAAGGGCGTTAAGGGTAAGTATAAGATGGTTGAT GGTGTCATGAAGAATGAACAAAGAGCCCTAAGACGTATCGCAAAGAAGCATCACAAGAAA AAGTAG

Protein Properties

Pfam Domain Function

FtsJ (PF01728 )
Spb1_C (PF07780 )

Protein Residues

841

Protein Molecular Weight

96484.0

Protein Theoretical pI

8.29

Signalling Regions

None

Transmembrane Regions

None

Protein Sequence

>AdoMet-dependent rRNA methyltransferase SPB1 MGKTQKKNSKGRLDRYYYLAKEKGYRARSSFKIIQINEKYGHFLEKSKVVIDLCAAPGSW CQVASKLCPVNSLIIGVDIVPMKPMPNVITFQSDITTEDCRSKLRGYMKTWKADTVLHDG APNVGLGWVQDAFTQSQLTLQALKLAVENLVVNGTFVTKIFRSKDYNKLIWVFQQLFEKV EATKPPASRNVSAEIFVVCKGFKAPKRLDPRLLDPKEVFEELPDGQQNMESKIYNPEKKV RKRQGYEEGDNLLYHETSILDFVRTEDPISMLGEMNKFTIDENDHEWKILKKLKQTTDEF RSCIEDLKVLGKKDFKMILRWRKIAREILGIEVKDDAKTEIEVVPLTEEEQIEKDLQGLQ EKQRLNVKRERRRKNEMKQKELQRMQMNMITPTDIGIEAASLGKESLFNLKTAEKTGILN DLAKGKKRMIFTDDELAKDNDIYIDENIMIKDKDSAADADDLESELNAMYSDYKTRRSER DAKFRAKQARGGDNEEEWTGFNEGSLEKKEEEGKDYIEDNDDEGVEGDSDDDEAITNLIS KLKGQEGDHKLSSKARMIFNDPIFNNVEPDLPVNTVNDGIMSSESVGDISKLNKKRKHEE MHQKQDEADSSDESSSDDSDFEIVANDNASEEFDSDYDSEEEKNQTKKEKHSRDIDIATV EAMTLAHQLALGQKNKHDLVDEGFNRYTFRDTENLPDWFLEDEKEHSKINKPITKEAAMA IKEKIKAMNARPIKKVAEAKARKRMRAVARLEKIKKKAGLINDDSDKTEKDKAEEISRLM RKVTKKPKTKPKVTLVVASGRNKGLAGRPKGVKGKYKMVDGVMKNEQRALRRIAKKHHKK K

References

External Links

Resource	Link
Saccharomyces Genome Database	SPB1
Uniprot ID	P25582
Uniprot Name	SPB1_YEAST
GenBank Gene ID	X59720
Genebank Protein ID	1907120

General Reference

Oliver, S. G., van der Aart, Q. J., Agostoni-Carbone, M. L., Aigle, M., Alberghina, L., Alexandraki, D., Antoine, G., Anwar, R., Ballesta, J. P., Benit, P., et, a. l. .. (1992). "The complete DNA sequence of yeast chromosome III." Nature 357:38-46.1574125
Defoor, E., Debrabandere, R., Keyers, B., Voet, M., Volckaert, G. (1992). "Nucleotide sequence of D10B, a BamHI fragment on the small-ring chromosome III of Saccharomyces cerevisiae." Yeast 8:681-687.1441748
Kressler, D., Rojo, M., Linder, P., Cruz, J. (1999). "Spb1p is a putative methyltransferase required for 60S ribosomal subunit biogenesis in Saccharomyces cerevisiae." Nucleic Acids Res 27:4598-4608.10556316
Pintard, L., Kressler, D., Lapeyre, B. (2000). "Spb1p is a yeast nucleolar protein associated with Nop1p and Nop58p that is able to bind S-adenosyl-L-methionine in vitro." Mol Cell Biol 20:1370-1381.10648622
Nissan, T. A., Bassler, J., Petfalski, E., Tollervey, D., Hurt, E. (2002). "60S pre-ribosome formation viewed from assembly in the nucleolus until export to the cytoplasm." EMBO J 21:5539-5547.12374754
Bonnerot, C., Pintard, L., Lutfalla, G. (2003). "Functional redundancy of Spb1p and a snR52-dependent mechanism for the 2'-O-ribose methylation of a conserved rRNA position in yeast." Mol Cell 12:1309-1315.14636587
Lapeyre, B., Purushothaman, S. K. (2004). "Spb1p-directed formation of Gm2922 in the ribosome catalytic center occurs at a late processing stage." Mol Cell 16:663-669.15546625
Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956