tRNA dimethylallyltransferase, mitochondrial (P07884)

Identification
NametRNA dimethylallyltransferase, mitochondrial
Synonyms
  • Isopentenyl-diphosphate: tRNA isopentenyltransferase
  • IPP transferase
  • IPPT
  • tRNA isopentenyltransferase
  • IPTase
Gene NameMOD5
Enzyme Class
Biological Properties
General FunctionInvolved in ATP binding
Specific FunctionCatalyzes the transfer of a dimethylallyl group onto the adenine at position 37 of both cytosolic and mitochondrial tRNAs, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)
Cellular LocationMitochondrion. Cytoplasm. Nucleus
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00095DimethylallylpyrophosphateShow
YMDB00219PyrophosphateShow
GO Classification
Component
Not Available
Function
binding
nucleoside binding
purine nucleoside binding
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
Process
tRNA metabolic process
tRNA processing
metabolic process
macromolecule metabolic process
cellular macromolecule metabolic process
RNA metabolic process
ncRNA metabolic process
Gene Properties
Chromosome Locationchromosome 15
LocusYOR274W
Gene Sequence>1287 bp ATGCTAAAGGGACCGCTTAAAGGTTGCTTAAATATGTCTAAAAAAGTTATAGTGATTGCT GGTACAACAGGAGTAGGAAAGTCTCAGTTGTCCATACAATTGGCACAAAAATTTAATGGC GAAGTTATCAATTCGGATTCTATGCAGGTATATAAAGATATTCCTATTATAACAAATAAA CATCCGCTTCAAGAAAGAGAGGGCATTCCTCACCATGTAATGAATCACGTAGATTGGTCA GAAGAATACTATTCCCATCGTTTTGAAACTGAATGCATGAACGCTATTGAAGATATTCAC CGGAGGGGTAAGATACCCATCGTTGTTGGGGGTACTCATTATTATTTGCAGACTCTTTTC AATAAGCGGGTCGATACGAAATCATCCGAACGTAAGCTCACAAGGAAGCAGCTCGATATT TTGGAATCGACTGACCCTGATGTGATTTATAACACACTTGTGAAGTGTGATCCTGATATA GCTACCAAATACCATCCTAATGACTACAGGAGGGTACAACGAATGTTGGAAATCTACTAT AAGACGGGCAAAAAACCAAGCGAGACATTTAACGAACAAAAAATAACTTTGAAGTTTGAT ACCCTATTTTTATGGCTATACAGTAAGCCGGAGCCACTTTTCCAAAGGCTTGACGATCGT GTAGACGACATGCTAGAAAGAGGCGCCTTGCAAGAAATCAAGCAATTATATGAATATTAC AGTCAAAATAAATTTACGCCGGAGCAATGCGAGAATGGTGTTTGGCAAGTTATTGGCTTC AAAGAATTCTTACCGTGGCTAACCGGGAAAACTGATGACAACACAGTAAAATTGGAGGAT TGTATAGAAAGAATGAAAACGAGAACACGTCAATACGCAAAAAGGCAGGTAAAATGGATC AAGAAAATGCTGATACCTGACATCAAAGGCGATATATACTTACTGGATGCCACTGACCTT TCTCAATGGGACACGAATGCATCTCAAAGAGCGATAGCTATTTCCAATGACTTTATTAGC AATCGACCCATCAAGCAAGAAAGGGCACCTAAAGCCCTAGAGGAGCTGTTGTCTAAAGGC GAAACTACGATGAAGAAATTGGACGATTGGACACATTATACTTGCAACGTCTGTCGTAAC GCAGATGGTAAGAACGTTGTAGCTATTGGCGAAAAATATTGGAAAATTCATTTGGGTAGC AGAAGACATAAATCCAACTTGAAAAGAAACACTCGTCAAGCTGATTTTGAAAAATGGAAA ATAAACAAGAAGGAGACTGTGGAATGA
Protein Properties
Pfam Domain Function
Protein Residues428
Protein Molecular Weight50236.19922
Protein Theoretical pI9.41
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>tRNA dimethylallyltransferase, mitochondrial MLKGPLKGCLNMSKKVIVIAGTTGVGKSQLSIQLAQKFNGEVINSDSMQVYKDIPIITNK HPLQEREGIPHHVMNHVDWSEEYYSHRFETECMNAIEDIHRRGKIPIVVGGTHYYLQTLF NKRVDTKSSERKLTRKQLDILESTDPDVIYNTLVKCDPDIATKYHPNDYRRVQRMLEIYY KTGKKPSETFNEQKITLKFDTLFLWLYSKPEPLFQRLDDRVDDMLERGALQEIKQLYEYY SQNKFTPEQCENGVWQVIGFKEFLPWLTGKTDDNTVKLEDCIERMKTRTRQYAKRQVKWI KKMLIPDIKGDIYLLDATDLSQWDTNASQRAIAISNDFISNRPIKQERAPKALEELLSKG ETTMKKLDDWTHYTCNVCRNADGKNVVAIGEKYWKIHLGSRRHKSNLKRNTRQADFEKWK INKKETVE
References
External Links
ResourceLink
Saccharomyces Genome Database MOD5
Uniprot IDP07884
Uniprot NameMOD5_YEAST
GenBank Gene IDX89633
Genebank Protein ID1419759
General Reference
  • Najarian, D., Dihanich, M. E., Martin, N. C., Hopper, A. K. (1987). "DNA sequence and transcript mapping of MOD5: features of the 5' region which suggest two translational starts." Mol Cell Biol 7:185-191.3031457
  • Cheret, G., Bernardi, A., Sor, F. (1996). "DNA sequence analysis of the VPH1-SNF2 region on chromosome XV of Saccharomyces cerevisiae." Yeast 12:1059-1064.8896271
  • Dujon, B., Albermann, K., Aldea, M., Alexandraki, D., Ansorge, W., Arino, J., Benes, V., Bohn, C., Bolotin-Fukuhara, M., Bordonne, R., Boyer, J., Camasses, A., Casamayor, A., Casas, C., Cheret, G., Cziepluch, C., Daignan-Fornier, B., Dang, D. V., de Haan, M., Delius, H., Durand, P., Fairhead, C., Feldmann, H., Gaillon, L., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Nature 387:98-102.9169874
  • Slusher, L. B., Gillman, E. C., Martin, N. C., Hopper, A. K. (1991). "mRNA leader length and initiation codon context determine alternative AUG selection for the yeast gene MOD5." Proc Natl Acad Sci U S A 88:9789-9793.1946403
  • Gillman, E. C., Slusher, L. B., Martin, N. C., Hopper, A. K. (1991). "MOD5 translation initiation sites determine N6-isopentenyladenosine modification of mitochondrial and cytoplasmic tRNA." Mol Cell Biol 11:2382-2390.1850093
  • Boguta, M., Hunter, L. A., Shen, W. C., Gillman, E. C., Martin, N. C., Hopper, A. K. (1994). "Subcellular locations of MOD5 proteins: mapping of sequences sufficient for targeting to mitochondria and demonstration that mitochondrial and nuclear isoforms commingle in the cytosol." Mol Cell Biol 14:2298-2306.8139535
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Zhou, C., Huang, R. H. (2008). "Crystallographic snapshots of eukaryotic dimethylallyltransferase acting on tRNA: insight into tRNA recognition and reaction mechanism." Proc Natl Acad Sci U S A 105:16142-16147.18852462