Phosphatidylinositol 4,5-bisphosphate-binding protein SLM2 (P53955)

Identification
NamePhosphatidylinositol 4,5-bisphosphate-binding protein SLM2
Synonyms
  • Synthetic lethal with MSS4 protein 2
  • TORC2 effector protein SLM2
Gene NameSLM2
Enzyme ClassNot Available
Biological Properties
General FunctionInvolved in phosphoinositide binding
Specific FunctionTogether with SLM1, effector of the TORC2- and calcineurin-signaling pathways. Phosphorylated and activated by TORC2 under favorable growth conditions. Mediates actin polarization via inhibition of calcineurin-dependent transcription. Upon nutrient limitation or environmental stress, gets dephosphorylated by calcineurin, inhibiting interaction with TORC2, thereby antagonizing TORC2 signaling and mediating calcineurin-dependent actin depolarization. Also functions in heat-induced, calcineurin-mediated uracil permease (FUR4) endocytosis
Cellular LocationCell membrane; Peripheral membrane protein; Cytoplasmic side
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
GO ClassificationNot Available
Gene Properties
Chromosome LocationNot Available
Locus
Gene Sequence>1971 bp ATGTCTTACCAACGGAACAGTGCAAGAGCCTCTCTTGACCTCAGAAGTCAATATCAACAA CTGGAAGGCAGAATGAGGTCAGAACATTTTAATCCCGCCTATCAACAGCAGCAACAAAAA GGCCAGAACATACCCCTGTCACTACCCTCGTCGCTTGCGCAACGCAATCCTATACCTTAC CCTATCGACGCTGTGACTTCCGATCCCACTATCCCGGCTCAGTTGAACGTATATGATCAC GATCGGCAAAATTCGATAGTCGATGCTGCGGCTGGCACCAATACGACGCACTCTTTGAAC TCTAATAATATTCCATCATCACAAAATAATAATATTAATAATAACAATATCAATAATGTT GGTTCCTTCACTGACCCTTCCATGCTAACGCTGCCAAAAATGTCTTTACATAGCCACCAG AAGCAGTATGATTCTAACCAAAACGATCCTAGGTCCCCTCTGGCGATACTGATACCGACT TCTGCGCAGCCCACTGATGTCTTGTCTGCTAGGTTTTCTGCCTGGAGAAATGTTATTCGT GCCATCTTGGTATATCTCTCTGAGACCGCCTCAATACAGGACGAAATAGTTAGACAGCAA CTGAGGTTGTCCCATGCTGTGCAGTTCCCCTTTTTCAGCATTGAAAACCAATATCAACCG GTCTCTAATGAAGACAAGTCCATGCAGAAATTTTTTCTGCCCTTAGGAAGTGGATCCGTT CAAGATTTGCCCACGATGCTGACCAAGTATCATGATAATCTGGCCTCTTTGGCATCGAAA AGTTCCAAGGAGTTGACCAGTGAAATTATACCCAGGCTAGAGGATTTGAGAAGAGATTTA CTAGTCAAGATTAAAGAAATTAAGGCTTTGCAATCTGATTTTAAAAATTCCTGTAACAAA GAGCTTCAGCAAACTAAACACTTGATGAAGCTTTTTAATGAATCATTAAAAGAATGTAAA TTAGGTACACCAAAGAGTGACCCATTTTTGATAAAATTGCAATTGGAAAAGCAAATCAAA AGACAGTTGGTGGAAGAAAATTATTTGCACGAGGCATTCGATAACTTGCAGAATTCAGGG GCCCAGTTAGAAAGTGTTATTGTAATGGAAATACAGAACGGATTGACCAGCTACGCTAGA ATTCTTGGCAAAGAAGCCCAAGTTGTATTTGACTCAGTTATCTCTAAATTAGATTCCACG ATACTGAACAAAAACACGAATCTTGAATGGGATAGTTTCATTCTAAGGAATATTTCCAAT TTTGTGCCTCCAAATTTACCCATGAGGAGGTTTAAAGAGATCTCGTATAGCAATCAAAAC GATCCTTTTACTTTTGAAGTTAAATCTGGGTTTCTGGAGAAAAGATCAAAATTTTTAAAG TCATACTCACGTGGGTTTTATGTCCTCACTCCAAGCTTTTTACATGAATTTAAGACACCA GATAAGCATAAATTTTCCACACCGTTAATGTCAATACCATTAGTGGAATGTACTGTCACT GAGCATTCCAAAAAGACGAAATCCAACTCAGAGCAGGGCAAGAACAAGTTTATATTGCGT ACGAATTCTAACGGACTAATACATAGGGGTCACAACTGGGTATTCAAAGTAGACTCTTAT GACGATATGATAGAATGGTTTGGAAATATCAAAGCCTTATCAAGTTTGCCCAACTATGAC GACAAATGCAAATATGTGAGTAAAGTTGCCAAACTGTCTAAAGAAAAAGCCAAGAGTAAT GAAAACACCACGGAGAGCGTGACCCCCCAGGTAACTAATGAGCAACATACGAGATACGAT GATGTGAGTAGTAGCAATTTTCCCTTGAATTCCATTCCAAAATTAGACAATTTGACAATC ACTAACACCACGTCATCGATACCGGAAACGAATGATTCACAAATTCAGAATAGGGTTCCT GAATTTTACATTGAAAATGTAGATTCCCCTAGAAAAAGCAATCAGCTGTGA
Protein Properties
Pfam Domain Function
Protein Residues656
Protein Molecular Weight74784.60156
Protein Theoretical pI9.02
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Phosphatidylinositol 4,5-bisphosphate-binding protein SLM2 MSYQRNSARASLDLRSQYQQLEGRMRSEHFNPAYQQQQQKGQNIPLSLPSSLAQRNPIPY PIDAVTSDPTIPAQLNVYDHDRQNSIVDAAAGTNTTHSLNSNNIPSSQNNNINNNNINNV GSFTDPSMLTLPKMSLHSHQKQYDSNQNDPRSPLAILIPTSAQPTDVLSARFSAWRNVIR AILVYLSETASIQDEIVRQQLRLSHAVQFPFFSIENQYQPVSNEDKSMQKFFLPLGSGSV QDLPTMLTKYHDNLASLASKSSKELTSEIIPRLEDLRRDLLVKIKEIKALQSDFKNSCNK ELQQTKHLMKLFNESLKECKLGTPKSDPFLIKLQLEKQIKRQLVEENYLHEAFDNLQNSG AQLESVIVMEIQNGLTSYARILGKEAQVVFDSVISKLDSTILNKNTNLEWDSFILRNISN FVPPNLPMRRFKEISYSNQNDPFTFEVKSGFLEKRSKFLKSYSRGFYVLTPSFLHEFKTP DKHKFSTPLMSIPLVECTVTEHSKKTKSNSEQGKNKFILRTNSNGLIHRGHNWVFKVDSY DDMIEWFGNIKALSSLPNYDDKCKYVSKVAKLSKEKAKSNENTTESVTPQVTNEQHTRYD DVSSSNFPLNSIPKLDNLTITNTTSSIPETNDSQIQNRVPEFYIENVDSPRKSNQL
References
External Links
ResourceLink
Saccharomyces Genome Database SLM2
Uniprot IDP53955
Uniprot NameSLM2_YEAST
GenBank Gene IDAY692658
Genebank Protein ID51012767
General Reference
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  • Yu, J. W., Mendrola, J. M., Audhya, A., Singh, S., Keleti, D., DeWald, D. B., Murray, D., Emr, S. D., Lemmon, M. A. (2004). "Genome-wide analysis of membrane targeting by S. cerevisiae pleckstrin homology domains." Mol Cell 13:677-688.15023338
  • Fadri, M., Daquinag, A., Wang, S., Xue, T., Kunz, J. (2005). "The pleckstrin homology domain proteins Slm1 and Slm2 are required for actin cytoskeleton organization in yeast and bind phosphatidylinositol-4,5-bisphosphate and TORC2." Mol Biol Cell 16:1883-1900.15689497
  • Bultynck, G., Heath, V. L., Majeed, A. P., Galan, J. M., Haguenauer-Tsapis, R., Cyert, M. S. (2006). "Slm1 and slm2 are novel substrates of the calcineurin phosphatase required for heat stress-induced endocytosis of the yeast uracil permease." Mol Cell Biol 26:4729-4745.16738335
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