Target of rapamycin complex subunit LST8 (P41318)

Identification
NameTarget of rapamycin complex subunit LST8
Synonyms
  • TORC subunit LST8
  • Lethal with SEC13 protein 8
Gene NameLST8
Enzyme ClassNot Available
Biological Properties
General FunctionInvolved in protein binding
Specific FunctionEssential component of both TORC1 and TORC2. TORC1 regulates multiple cellular processes to control cell growth in response to environmental signals. Nutrient limitation and environmental stress signals cause inactivation of TORC1. Active TORC1 positively controls ribosome biogenesis via control of rRNA, ribosomal protein and tRNA gene expression, and rRNA processing. TORC1 positively controls protein biosynthesis by regulation of mRNA stability, translation initiation factor activity, and high- affinity amino acid permeases that serve to provide amino acids for use by the translation machinery. TORC1 also promotes growth by sequestering a number of nutrient and general stress-responsive transcription factors in the cytoplasm. TORC1 negatively controls macroautophagy, a process to recycle surplus cytoplasmic mass under nutrient starvation conditions. LST8 is involved in the negative regulation of transcription factors GLN3 and RTG1-RTG3, limiting the synthesis of alpha-ketoglutarate, glutamate and glutamine. LST8 is required for targeting of amino acid permeases (AAPs) to the plasma membrane. TORC2 regulates cell cycle- dependent polarization of the actin-cytoskeleton, cell wall integrity, and receptor endocytosis. TORC2 controls polarity of the actin cytoskeleton, which is required for orienting the secretory pathway toward discrete growth sites, via the RHO1/PKC1/MAPK cell integrity pathway. LST8 is involved in maintenance of cell wall integrity. LST8 modulates TOR2 kinase activity
Cellular LocationCell membrane; Peripheral membrane protein; Cytoplasmic side. Vacuole membrane; Peripheral membrane protein; Cytoplasmic side.
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
GO ClassificationNot Available
Gene Properties
Chromosome LocationNot Available
Locus
Gene Sequence>912 bp ATGTCTGTTATCTTAGTATCTGCTGGCTATGACCACACAATAAGATTTTGGGAGGCTCTT ACTGGGGTTTGTTCAAGAACAATTCAGCATTCCGACTCACAGGTTAATCGCTTAGAAATC ACCAACGATAAAAAGTTGTTGGCAACAGCAGGCCATCAAAACGTACGGCTTTATGACATT CGGACGACAAATCCCAATCCAGTAGCATCCTTTGAGGGTCATCGGGGAAACGTTACCTCC GTTTCTTTTCAACAGGACAACAGATGGATGGTGACCTCTAGCGAAGATGGGACCATTAAA GTTTGGGATGTGCGTTCTCCGTCCATTCCTAGAAATTACAAACATAACGCACCAGTAAAT GAAGTCGTAATTCATCCTAACCAAGGCGAGTTGATATCTTGTGATAGAGATGGTAATATA AGAATTTGGGACTTAGGAGAAAATCAGTGTACCCATCAATTGACACCTGAGGACGATACC TCTCTCCAGTCTTTATCTATGGCAAGTGATGGTTCGATGTTAGCGGCAGCAAATACTAAA GGTAATTGTTACGTATGGGAAATGCCAAATCATACTGACGCATCCCATCTAAAACCGGTA ACAAAATTCAGAGCGCACTCCACATATATAACTAGAATTCTACTATCATCTGATGTAAAA CATTTGGCAACATGTTCAGCGGATCACACTGCTCGTGTGTGGTCGATTGATGATGATTTT AAGCTAGAAACGACATTGGATGGCCACCAAAGGTGGGTTTGGGATTGCGCATTTAGTGCT GACAGTGCGTATCTAGTAACGGCATCGTCCGATCATTACGTGAGATTATGGGATTTATCA ACTAGAGAAATTGTCAGACAGTACGGTGGACACCACAAGGGAGCTGTATGTGTCGCATTA AACGATGTATAG
Protein Properties
Pfam Domain Function
Protein Residues303
Protein Molecular Weight34033.60156
Protein Theoretical pI6.5
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Target of rapamycin complex subunit LST8 MSVILVSAGYDHTIRFWEALTGVCSRTIQHSDSQVNRLEITNDKKLLATAGHQNVRLYDI RTTNPNPVASFEGHRGNVTSVSFQQDNRWMVTSSEDGTIKVWDVRSPSIPRNYKHNAPVN EVVIHPNQGELISCDRDGNIRIWDLGENQCTHQLTPEDDTSLQSLSMASDGSMLAAANTK GNCYVWEMPNHTDASHLKPVTKFRAHSTYITRILLSSDVKHLATCSADHTARVWSIDDDF KLETTLDGHQRWVWDCAFSADSAYLVTASSDHYVRLWDLSTREIVRQYGGHHKGAVCVAL NDV
References
External Links
ResourceLink
Saccharomyces Genome Database LST8
Uniprot IDP41318
Uniprot NameLST8_YEAST
GenBank Gene IDX77114
Genebank Protein ID496716
General Reference
  • Verhasselt, P., Aert, R., Voet, M., Volckaert, G. (1994). "Nucleotide sequence analysis of an 8887 bp region of the left arm of yeast chromosome XIV, encompassing the centromere sequence." Yeast 10:945-951.7985421
  • Philippsen, P., Kleine, K., Pohlmann, R., Dusterhoft, A., Hamberg, K., Hegemann, J. H., Obermaier, B., Urrestarazu, L. A., Aert, R., Albermann, K., Altmann, R., Andre, B., Baladron, V., Ballesta, J. P., Becam, A. M., Beinhauer, J., Boskovic, J., Buitrago, M. J., Bussereau, F., Coster, F., Crouzet, M., D'Angelo, M., Dal Pero, F., De Antoni, A., Del Rey, F., Doignon, F., Domdey, H., Dubois, E., Fiedler, T., Fleig, U., Floeth, M., Fritz, C., Gaillardin, C., Garcia-Cantalejo, J. M., Glansdorff, N. N., Goffeau, A., Gueldener, U., Herbert, C., Heumann, K., Heuss-Neitzel, D., Hilbert, H., Hinni, K., Iraqui Houssaini, I., Jacquet, M., Jimenez, A., Jonniaux, J. L., Karpfinger, L., Lanfranchi, G., Lepingle, A., Levesque, H., Lyck, R., Maftahi, M., Mallet, L., Maurer, K. C., Messenguy, F., Mewes, H. W., Mosti, D., Nasr, F., Nicaud, J. M., Niedenthal, R. K., Pandolfo, D., Pierard, A., Piravandi, E., Planta, R. J., Pohl, T. M., Purnelle, B., Rebischung, C., Remacha, M., Revuelta, J. L., Rinke, M., Saiz, J. E., Sartorello, F., Scherens, B., Sen-Gupta, M., Soler-Mira, A., Urbanus, J. H., Valle, G., Van Dyck, L., Verhasselt, P., Vierendeels, F., Vissers, S., Voet, M., Volckaert, G., Wach, A., Wambutt, R., Wedler, H., Zollner, A., Hani, J. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Nature 387:93-98.9169873
  • Roberg, K. J., Bickel, S., Rowley, N., Kaiser, C. A. (1997). "Control of amino acid permease sorting in the late secretory pathway of Saccharomyces cerevisiae by SEC13, LST4, LST7 and LST8." Genetics 147:1569-1584.9409822
  • Liu, Z., Sekito, T., Epstein, C. B., Butow, R. A. (2001). "RTG-dependent mitochondria to nucleus signaling is negatively regulated by the seven WD-repeat protein Lst8p." EMBO J 20:7209-7219.11742997
  • Loewith, R., Jacinto, E., Wullschleger, S., Lorberg, A., Crespo, J. L., Bonenfant, D., Oppliger, W., Jenoe, P., Hall, M. N. (2002). "Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control." Mol Cell 10:457-468.12408816
  • Chen, E. J., Kaiser, C. A. (2003). "LST8 negatively regulates amino acid biosynthesis as a component of the TOR pathway." J Cell Biol 161:333-347.12719473
  • Wedaman, K. P., Reinke, A., Anderson, S., Yates, J. 3rd, McCaffery, J. M., Powers, T. (2003). "Tor kinases are in distinct membrane-associated protein complexes in Saccharomyces cerevisiae." Mol Biol Cell 14:1204-1220.12631735
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Wullschleger, S., Loewith, R., Oppliger, W., Hall, M. N. (2005). "Molecular organization of target of rapamycin complex 2." J Biol Chem 280:30697-30704.16002396