Peroxiredoxin DOT5 (P40553)

Identification
NamePeroxiredoxin DOT5
Synonyms
  • Disrupter of telomere silencing protein 5
  • Nuclear thiol peroxidase
  • nTPx
  • Thioredoxin reductase
Gene NameDOT5
Enzyme Class
Biological Properties
General FunctionPosttranslational modification, protein turnover, chaperones
Specific FunctionHas a role in telomere silencing, which is the repression of chromatin structure which leads to a stop in the transcription of nearby genes. Also has a role in the regulation of telomere length. Acts as an alkyl-hydroperoxide reductase in the nucleus during post-diauxic growth. Preferentially reduces alkyl-hydroperoxides rather than hydrogen preoxide. Acts as an antioxidant necessary for stationary phase survival
Cellular LocationNucleus. Chromosome, telomere (Potential)
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
GO Classification
Function
catalytic activity
oxidoreductase activity
antioxidant activity
Process
cellular process
cellular homeostasis
cell redox homeostasis
Gene Properties
Chromosome LocationNot Available
Locus
Gene Sequence>648 bp ATGGGTGAAGCACTACGTAGATCAACCAGGATTGCAATATCCAAAAGAATGTTGGAAGAG GAAGAGTCCAAACTGGCCCCTATTTCGACACCGGAAGTGCCTAAGAAGAAAATCAAGACG GGTCCTAAACATAACGCTAATCAAGCAGTAGTTCAAGAGGCAAATAGGTCATCTGATGTT AACGAATTAGAGATAGGCGATCCTATTCCTGATTTGAGTCTTTTAAATGAAGATAATGAC TCTATCTCCTTGAAGAAAATCACCGAAAATAACAGAGTTGTGGTGTTTTTTGTGTATCCC AGGGCAAGCACGCCTGGTTGTACTAGACAGGCCTGTGGATTTCGTGACAATTACCAGGAA CTCAAGAAATATGCTGCTGTCTTTGGACTGAGTGCAGATTCTGTGACATCCCAGAAAAAG TTTCAGAGTAAACAAAATTTGCCATATCATTTACTAAGCGATCCAAAGAGAGAGTTTATT GGGTTGCTAGGAGCCAAAAAAACGCCACTTTCTGGTTCTATTAGATCGCATTTCATTTTT GTTGATGGGAAGTTAAAATTCAAAAGAGTTAAGATATCACCAGAAGTTAGTGTAAATGAC GCCAAAAAGGAGGTTTTAGAAGTCGCTGAAAAGTTTAAAGAAGAATGA
Protein Properties
Pfam Domain Function
Protein Residues215
Protein Molecular Weight24119.40039
Protein Theoretical pI9.99
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Peroxiredoxin DOT5 MGEALRRSTRIAISKRMLEEEESKLAPISTPEVPKKKIKTGPKHNANQAVVQEANRSSDV NELEIGDPIPDLSLLNEDNDSISLKKITENNRVVVFFVYPRASTPGCTRQACGFRDNYQE LKKYAAVFGLSADSVTSQKKFQSKQNLPYHLLSDPKREFIGLLGAKKTPLSGSIRSHFIF VDGKLKFKRVKISPEVSVNDAKKEVLEVAEKFKEE
References
External Links
ResourceLink
Saccharomyces Genome Database DOT5
Uniprot IDP40553
Uniprot NameDOT5_YEAST
GenBank Gene IDAY558298
Genebank Protein ID45270486
General Reference
  • Singer, M. S., Kahana, A., Wolf, A. J., Meisinger, L. L., Peterson, S. E., Goggin, C., Mahowald, M., Gottschling, D. E. (1998). "Identification of high-copy disruptors of telomeric silencing in Saccharomyces cerevisiae." Genetics 150:613-632.9755194
  • Churcher, C., Bowman, S., Badcock, K., Bankier, A., Brown, D., Chillingworth, T., Connor, R., Devlin, K., Gentles, S., Hamlin, N., Harris, D., Horsnell, T., Hunt, S., Jagels, K., Jones, M., Lye, G., Moule, S., Odell, C., Pearson, D., Rajandream, M., Rice, P., Rowley, N., Skelton, J., Smith, V., Barrell, B., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Nature 387:84-87.9169870
  • Hu, Y., Rolfs, A., Bhullar, B., Murthy, T. V., Zhu, C., Berger, M. F., Camargo, A. A., Kelley, F., McCarron, S., Jepson, D., Richardson, A., Raphael, J., Moreira, D., Taycher, E., Zuo, D., Mohr, S., Kane, M. F., Williamson, J., Simpson, A., Bulyk, M. L., Harlow, E., Marsischky, G., Kolodner, R. D., LaBaer, J. (2007). "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Genome Res 17:536-543.17322287
  • Park, S. G., Cha, M. K., Jeong, W., Kim, I. H. (2000). "Distinct physiological functions of thiol peroxidase isoenzymes in Saccharomyces cerevisiae." J Biol Chem 275:5723-5732.10681558
  • Cha, M. K., Choi, Y. S., Hong, S. K., Kim, W. C., No, K. T., Kim, I. H. (2003). "Nuclear thiol peroxidase as a functional alkyl-hydroperoxide reductase necessary for stationary phase growth of Saccharomyces cerevisiae." J Biol Chem 278:24636-24643.12730197
  • Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Izawa, S., Kuroki, N., Inoue, Y. (2004). "Nuclear thioredoxin peroxidase Dot5 in Saccharomyces cerevisiae: roles in oxidative stress response and disruption of telomeric silencing." Appl Microbiol Biotechnol 64:120-124.12925864
  • Choi, J., Choi, S., Choi, J., Cha, M. K., Kim, I. H., Shin, W. (2005). "Crystallization and preliminary X-ray analysis of a truncated mutant of yeast nuclear thiol peroxidase, a novel atypical 2-Cys peroxiredoxin." Acta Crystallogr Sect F Struct Biol Cryst Commun 61:659-662.16511121
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956
  • Choi, J., Choi, S., Chon, J. K., Choi, J., Cha, M. K., Kim, I. H., Shin, W. (2005). "Crystal structure of the C107S/C112S mutant of yeast nuclear 2-Cys peroxiredoxin." Proteins 61:1146-1149.16245326