Vacuolar protein 8 (P39968)

Identification
NameVacuolar protein 8
SynonymsNot Available
Gene NameVAC8
Enzyme ClassNot Available
Biological Properties
General FunctionInvolved in protein anchor
Specific FunctionFunctions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole
Cellular LocationVacuole membrane; Lipid-anchor
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
GO Classification
Function
binding
Gene Properties
Chromosome LocationNot Available
Locus
Gene Sequence>1737 bp ATGGGTTCATGTTGTAGTTGCTTGAAAGATTCTTCAGACGAGGCCAGTGTCTCACCAATT GCCGATAATGAAAGAGAAGCGGTGACTTTATTACTAGGGTATTTGGAGGATAAAGACCAA TTAGACTTTTACTCTGGTGGTCCACTAAAGGCCCTAACTACTTTGGTGTATTCAGATAAT CTAAATTTGCAAAGGAGCGCCGCTCTGGCATTCGCAGAAATTACTGAAAAATACGTCCGT CAGGTCTCTAGAGAGGTACTGGAACCCATCTTAATATTATTACAAAGCCAAGATCCACAG ATTCAAGTGGCCGCTTGTGCAGCCTTGGGTAATTTGGCCGTTAATAATGAAAATAAATTA TTAATTGTGGAAATGGGAGGCTTAGAGCCATTAATAAACCAAATGATGGGTGACAATGTA GAAGTTCAATGTAATGCTGTTGGATGCATTACCAATTTAGCCACAAGGGATGACAATAAA CATAAGATTGCTACTTCTGGTGCTTTAATTCCATTGACTAAGCTAGCTAAATCCAAACAT ATAAGAGTTCAGAGAAATGCTACAGGTGCATTATTAAATATGACACATTCTGAGGAAAAT AGAAAAGAATTGGTTAATGCAGGTGCTGTGCCTGTATTGGTATCTTTACTGTCGTCCACC GATCCTGATGTTCAGTACTATTGTACTACTGCTTTATCAAATATAGCAGTGGATGAAGCC AATCGCAAGAAGTTGGCTCAAACTGAGCCAAGATTGGTTTCCAAATTGGTTAGTCTGATG GATTCTCCATCTTCAAGGGTAAAATGTCAAGCTACTTTGGCTTTACGAAACCTAGCGTCC GATACAAGCTACCAATTAGAAATTGTAAGGGCTGGTGGGTTGCCTCATTTGGTAAAGTTG ATTCAAAGTGATTCTATACCACTGGTTTTGGCTAGTGTGGCATGCATTAGAAACATTTCT ATTCACCCTCTAAATGAAGGTTTAATTGTTGATGCAGGTTTCTTAAAACCATTAGTAAGA TTGTTGGACTATAAGGATTCAGAAGAAATTCAATGTCACGCCGTATCTACTTTGAGAAAT TTGGCTGCATCATCTGAAAAAAACAGAAAAGAGTTCTTCGAAAGTGGTGCTGTCGAAAAA TGTAAGGAACTGGCTCTAGATTCACCTGTTAGTGTTCAAAGTGAAATTTCCGCATGTTTT GCTATTTTAGCCCTAGCAGATGTCTCCAAATTGGATCTACTGGAAGCAAATATCTTAGAT GCTTTAATCCCTATGACTTTTTCCCAGAACCAAGAAGTTTCTGGTAATGCTGCTGCTGCT TTGGCAAATCTATGTTCAAGAGTGAATAATTATACAAAAATTATTGAAGCATGGGATCGT CCGAATGAAGGCATTCGTGGATTTCTGATTAGATTTTTGAAAAGTGACTATGCTACCTTT GAACACATTGCGCTATGGACAATTTTACAATTGCTAGAAAGTCATAATGATAAAGTGGAA GATTTGGTTAAAAATGATGACGACATCATTAACGGAGTGAGAAAAATGGCAGATGTGACC TTTGAGCGTTTACAAAGATCAGGAATTGATGTTAAAAATCCGGGGAGCAACAACAATCCA AGCTCGAATGATAACAACAGTAATAACAATGACACAGGATCTGAACATCAACCTGTAGAG GATGCAAGTTTGGAATTGTATAATATTACTCAACAGATTTTACAATTTTTACATTGA
Protein Properties
Pfam Domain Function
Protein Residues578
Protein Molecular Weight63207.10156
Protein Theoretical pI4.76
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Vacuolar protein 8 MGSCCSCLKDSSDEASVSPIADNEREAVTLLLGYLEDKDQLDFYSGGPLKALTTLVYSDN LNLQRSAALAFAEITEKYVRQVSREVLEPILILLQSQDPQIQVAACAALGNLAVNNENKL LIVEMGGLEPLINQMMGDNVEVQCNAVGCITNLATRDDNKHKIATSGALIPLTKLAKSKH IRVQRNATGALLNMTHSEENRKELVNAGAVPVLVSLLSSTDPDVQYYCTTALSNIAVDEA NRKKLAQTEPRLVSKLVSLMDSPSSRVKCQATLALRNLASDTSYQLEIVRAGGLPHLVKL IQSDSIPLVLASVACIRNISIHPLNEGLIVDAGFLKPLVRLLDYKDSEEIQCHAVSTLRN LAASSEKNRKEFFESGAVEKCKELALDSPVSVQSEISACFAILALADVSKLDLLEANILD ALIPMTFSQNQEVSGNAAAALANLCSRVNNYTKIIEAWDRPNEGIRGFLIRFLKSDYATF EHIALWTILQLLESHNDKVEDLVKNDDDIINGVRKMADVTFERLQRSGIDVKNPGSNNNP SSNDNNSNNNDTGSEHQPVEDASLELYNITQQILQFLH
References
External Links
ResourceLink
Saccharomyces Genome Database VAC8
Uniprot IDP39968
Uniprot NameVAC8_YEAST
GenBank Gene IDAF005267
Genebank Protein ID33337489
General Reference
  • Dietrich, F. S., Mulligan, J., Hennessy, K., Yelton, M. A., Allen, E., Araujo, R., Aviles, E., Berno, A., Brennan, T., Carpenter, J., Chen, E., Cherry, J. M., Chung, E., Duncan, M., Guzman, E., Hartzell, G., Hunicke-Smith, S., Hyman, R. W., Kayser, A., Komp, C., Lashkari, D., Lew, H., Lin, D., Mosedale, D., Davis, R. W., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Nature 387:78-81.9169868
  • Wang, Y. X., Catlett, N. L., Weisman, L. S. (1998). "Vac8p, a vacuolar protein with armadillo repeats, functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole." J Cell Biol 140:1063-1074.9490720
  • Scott, S. V., Nice, D. C. 3rd, Nau, J. J., Weisman, L. S., Kamada, Y., Keizer-Gunnink, I., Funakoshi, T., Veenhuis, M., Ohsumi, Y., Klionsky, D. J. (2000). "Apg13p and Vac8p are part of a complex of phosphoproteins that are required for cytoplasm to vacuole targeting." J Biol Chem 275:25840-25849.10837477
  • Veit, M., Laage, R., Dietrich, L., Wang, L., Ungermann, C. (2001). "Vac8p release from the SNARE complex and its palmitoylation are coupled and essential for vacuole fusion." EMBO J 20:3145-3155.11406591
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Smotrys, J. E., Schoenfish, M. J., Stutz, M. A., Linder, M. E. (2005). "The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p." J Cell Biol 170:1091-1099.16186255
  • Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956