V-type proton ATPase subunit a, Golgi isoform (P37296)

Identification

Name

V-type proton ATPase subunit a, Golgi isoform

Synonyms

V-ATPase a 2 subunit
Similar to VPH1 protein 1
V-ATPase 101 kDa subunit
V-ATPase subunit AC115
Vacuolar proton translocating ATPase subunit a 2

Gene Name

STV1

Enzyme Class

Not Available

Biological Properties

General Function

Energy production and conversion

Specific Function

Subunit of the integral membrane V0 complex of vacuolar ATPase essential for assembly and catalytic activity. Is present only in Golgi- and endosome-residing V-ATPase complexes. Enzymes containing this subunit have a 4-fold lower ratio of proton transport to ATP hydrolysis than complexes containing the vacuolar isoform and do not dissociate V1 and V0 in response to glucose depletion. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells

Cellular Location

Endosome membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein

SMPDB Pathways

Not Available

KEGG Pathways

Not Available

SMPDB Reactions

Not Available

KEGG Reactions

Not Available

Metabolites

YMDB ID	Name	View

GO Classification

Component
macromolecular complex
protein complex
proton-transporting two-sector ATPase complex, proton-transporting domain
Function
transporter activity
transmembrane transporter activity
substrate-specific transmembrane transporter activity
ion transmembrane transporter activity
cation transmembrane transporter activity
inorganic cation transmembrane transporter activity
monovalent inorganic cation transmembrane transporter activity
hydrogen ion transmembrane transporter activity
Process
purine nucleoside triphosphate biosynthetic process
purine ribonucleoside triphosphate biosynthetic process
ATP biosynthetic process
ATP synthesis coupled proton transport
nitrogen compound metabolic process
cellular nitrogen compound metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleoside phosphate metabolic process
nucleotide metabolic process
purine nucleotide metabolic process
purine nucleotide biosynthetic process
metabolic process

Gene Properties

Chromosome Location

Not Available

Locus

Gene Sequence

>2673 bp ATGAATCAAGAAGAGGCTATATTCCGGTCAGCAGACATGACGTACGTCCAACTGTACATA CCGTTGGAAGTCATAAGAGAGGTAACTTTCTTATTAGGGAAAATGAGTGTCTTTATGGTG ATGGATCTCAATAAAGATTTAACTGCCTTTCAAAGAGGTTATGTTAACCAGTTGAGGCGT TTCGATGAAGTGGAAAGGATGGTAGGCTTCTTGAATGAGGTAGTTGAAAAGCACGCCGCA GAGACCTGGAAGTATATTTTACATATCGATGATGAAGGAAACGACATTGCCCAACCCGAT ATGGCGGATCTCATCAATACCATGGAACCACTATCGTTGGAAAATGTTAATGACATGGTG AAGGAAATTACTGATTGCGAATCCCGTGCAAGGCAATTAGATGAATCTTTAGATAGCCTT AGAAGTAAACTGAACGATCTTTTAGAGCAAAGGCAGGTAATATTCGAATGCTCAAAATTT ATTGAAGTCAATCCTGGGATTGCTGGAAGAGCTACAAACCCCGAGATTGAACAAGAAGAA AGGGATGTTGACGAGTTTAGAATGACCCCTGATGATATCAGTGAAACATTAAGCGATGCT TTTTCTTTTGACGACGAAACACCACAGGACCGGGGCGCTCTAGGGAATGACCTCACTAGA AACCAATCAGTCGAAGATCTAAGCTTTTTAGAGCAGGGATACCAGCATAGATACATGATA ACAGGCTCTATCAGAAGAACAAAAGTAGATATATTGAACAGAATCCTGTGGAGGTTATTG CGTGGTAATTTGATCTTTCAGAATTTTCCAATAGAGGAGCCATTGTTGGAAGGTAAAGAA AAGGTTGAAAAGGACTGCTTTATTATTTTTACTCATGGTGAAACATTGCTCAAGAAGGTT AAGCGTGTCATAGATTCCTTAAATGGTAAAATAGTCTCCCTGAATACTCGTTCTAGTGAA TTAGTTGACACCTTAAACCGTCAAATAGACGACTTGCAAAGAATCCTGGATACTACCGAA CAAACTTTACACACAGAACTGCTCGTTATACATGACCAGCTGCCAGTATGGTCCGCCATG ACGAAAAGGGAAAAATATGTTTATACCACATTGAACAAGTTCCAACAAGAATCACAGGGT CTAATAGCCGAAGGTTGGGTGCCTTCCACAGAATTGATCCATTTACAAGACTCATTGAAG GATTACATTGAAACGTTGGGTTCTGAATACAGTACCGTCTTTAATGTGATCCTAACCAAT AAATTACCACCTACATACCATAGAACCAACAAGTTCACTCAGGCCTTCCAATCGATTGTG GATGCATACGGTATCGCAACATATAAAGAAATCAATGCTGGTTTAGCCACGGTTGTCACC TTCCCATTTATGTTTGCCATTATGTTTGGTGACATGGGCCATGGTTTCATTTTATTTTTG ATGGCACTATTCTTGGTGCTAAATGAACGTAAGTTTGGTGCTATGCACAGGGACGAAATT TTTGACATGGCATTTACAGGTAGATATGTTTTATTGTTGATGGGTGCATTTTCTGTATAT ACTGGGCTGTTGTACAATGATATCTTTTCCAAATCTATGACAATATTCAAATCAGGCTGG CAATGGCCTTCCACTTTCAGAAAGGGCGAATCGATTGAGGCGAAGAAAACTGGGGTTTAT CCGTTTGGATTGGATTTTGCTTGGCATGGTACTGATAACGGACTACTATTTTCCAACTCT TATAAGATGAAGCTGTCCATTCTAATGGGATACGCACATATGACTTATTCCTTCATGTTT TCTTATATCAATTACAGAGCTAAAAACTCTAAAGTGGACATAATTGGTAACTTTATCCCC GGTTTAGTGTTTATGCAATCAATATTTGGTTATTTGTCGTGGGCTATCGTGTACAAGTGG TCGAAGGATTGGATTAAAGATGATAAGCCTGCTCCAGGGTTACTGAATATGCTAATAAAC ATGTTTTTAGCACCGGGTACTATTGATGATCAATTATATTCCGGACAAGCCAAGTTGCAA GTTGTACTACTGCTTGCGGCACTAGTTTGTGTCCCGTGGTTATTGCTGTATAAACCTTTA ACACTGAGAAGGTTAAATAAGAACGGTGGTGGTGGCAGGCCTCACGGATACCAAAGTGTG GGCAATATTGAGCATGAGGAACAAATAGCACAACAGAGACATTCAGCGGAAGGTTTCCAA GGGATGATCATCAGTGATGTTGCCAGTGTTGCAGACAGTATCAATGAAAGCGTTGGTGGA GGTGAACAGGGGCCATTTAATTTCGGCGATGTCATGATTCATCAGGTAATCCATACTATT GAATTCTGTTTAAATTGTATCTCTCATACAGCATCATATCTACGTCTTTGGGCACTATCG TTGGCGCATGCCGAATTATCTAGTGTTTTATGGGATATGACAATTTCAAACGCTTTCAGT TCTAAAAACTCAGGGTCGCCTTTGGCTGTCATGAAGGTGGTTTTCTTATTTGCTATGTGG TTTGTTTTAACCGTTTGTATCCTAGTTTTCATGGAAGGTACTTCCGCAATGTTGCATGCG CTACGTTTGCATTGGGTGGAGGCAATGTCCAAATTTTTTGAAGGTGAAGGATATGCGTAC GAGCCATTTTCATTTCGTGCAATAATAGAATAA

Protein Properties

Pfam Domain Function

V_ATPase_I (PF01496 )

Protein Residues

890

Protein Molecular Weight

101660.0

Protein Theoretical pI

5.04

Signalling Regions

None

Transmembrane Regions

467-487
509-529
585-605
618-638
646-666
681-701
796-816
833-853

Protein Sequence

>V-type proton ATPase subunit a, Golgi isoform MNQEEAIFRSADMTYVQLYIPLEVIREVTFLLGKMSVFMVMDLNKDLTAFQRGYVNQLRR FDEVERMVGFLNEVVEKHAAETWKYILHIDDEGNDIAQPDMADLINTMEPLSLENVNDMV KEITDCESRARQLDESLDSLRSKLNDLLEQRQVIFECSKFIEVNPGIAGRATNPEIEQEE RDVDEFRMTPDDISETLSDAFSFDDETPQDRGALGNDLTRNQSVEDLSFLEQGYQHRYMI TGSIRRTKVDILNRILWRLLRGNLIFQNFPIEEPLLEGKEKVEKDCFIIFTHGETLLKKV KRVIDSLNGKIVSLNTRSSELVDTLNRQIDDLQRILDTTEQTLHTELLVIHDQLPVWSAM TKREKYVYTTLNKFQQESQGLIAEGWVPSTELIHLQDSLKDYIETLGSEYSTVFNVILTN KLPPTYHRTNKFTQAFQSIVDAYGIATYKEINAGLATVVTFPFMFAIMFGDMGHGFILFL MALFLVLNERKFGAMHRDEIFDMAFTGRYVLLLMGAFSVYTGLLYNDIFSKSMTIFKSGW QWPSTFRKGESIEAKKTGVYPFGLDFAWHGTDNGLLFSNSYKMKLSILMGYAHMTYSFMF SYINYRAKNSKVDIIGNFIPGLVFMQSIFGYLSWAIVYKWSKDWIKDDKPAPGLLNMLIN MFLAPGTIDDQLYSGQAKLQVVLLLAALVCVPWLLLYKPLTLRRLNKNGGGGRPHGYQSV GNIEHEEQIAQQRHSAEGFQGMIISDVASVADSINESVGGGEQGPFNFGDVMIHQVIHTI EFCLNCISHTASYLRLWALSLAHAQLSSVLWDMTISNAFSSKNSGSPLAVMKVVFLFAMW FVLTVCILVFMEGTSAMLHALRLHWVEAMSKFFEGEGYAYEPFSFRAIIE

References

External Links

Resource	Link
Saccharomyces Genome Database	STV1
Uniprot ID	P37296
Uniprot Name	STV1_YEAST
GenBank Gene ID	U06465
Genebank Protein ID	460160

General Reference

Manolson, M. F., Wu, B., Proteau, D., Taillon, B. E., Roberts, B. T., Hoyt, M. A., Jones, E. W. (1994). "STV1 gene encodes functional homologue of 95-kDa yeast vacuolar H(+)-ATPase subunit Vph1p." J Biol Chem 269:14064-14074.7514599
Bowman, S., Churcher, C., Badcock, K., Brown, D., Chillingworth, T., Connor, R., Dedman, K., Devlin, K., Gentles, S., Hamlin, N., Hunt, S., Jagels, K., Lye, G., Moule, S., Odell, C., Pearson, D., Rajandream, M., Rice, P., Skelton, J., Walsh, S., Whitehead, S., Barrell, B. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Nature 387:90-93.9169872
Kawasaki-Nishi, S., Nishi, T., Forgac, M. (2001). "Yeast V-ATPase complexes containing different isoforms of the 100-kDa a-subunit differ in coupling efficiency and in vivo dissociation." J Biol Chem 276:17941-17948.11278748
Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
Gruhler, A., Olsen, J. V., Mohammed, S., Mortensen, P., Faergeman, N. J., Mann, M., Jensen, O. N. (2005). "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Mol Cell Proteomics 4:310-327.15665377
Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258
Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956