Inositol phosphorylceramide synthase (P36107)

Identification
NameInositol phosphorylceramide synthase
Synonyms
  • IPC synthase
  • Aureobasidin A resistance protein
  • Phosphatidylinositol:ceramide phosphoinositol transferase
Gene NameAUR1
Enzyme Class
Biological Properties
General FunctionInvolved in catalytic activity
Specific FunctionCatalyzes the addition of a phosphorylinositol group onto ceramide to form inositol phosphorylceramide, an essential step in sphingolipid biosynthesis
Cellular LocationGolgi apparatus, Golgi stack membrane; Multi-pass membrane protein
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG Reactions
1D-myo-inositol 1-phosphate + N-(24-hydroxytetracosanoyl)sphinganine ↔ inositol-P-ceramide C (C26)
1D-myo-inositol 1-phosphate + N-(26-hydroxyhexacosanoyl)sphinganine ↔ inositol-P-ceramide C (C26)
N-(24-Hydroxytetracosanoyl)phytosphingosine + 1D-myo-inositol 1-phosphate ↔ inositol-P-ceramide C (C26)
N-(26-Hydroxyhexacosanyl)phytosphingosine + 1D-myo-inositol 1-phosphate ↔ inositol-P-ceramide C (C26)
Metabolites
YMDB IDNameView
YMDB00620N-(24-hydroxytetracosanoyl)sphinganineShow
YMDB00622N-(26-hydroxyhexacosanoyl)sphinganineShow
YMDB00623N-(24-Hydroxytetracosanoyl)phytosphingosineShow
YMDB00624N-(26-Hydroxyhexacosanyl)phytosphingosineShow
YMDB00712inositol-P-ceramide C (C26)Show
YMDB007141D-myo-inositol 1-phosphateShow
GO Classification
Component
cell part
membrane
Function
catalytic activity
Process
Not Available
Gene Properties
Chromosome Locationchromosome 11
LocusYKL004W
Gene Sequence>1206 bp ATGGCAAACCCTTTTTCGAGATGGTTTCTATCAGAGAGACCTCCAAACTGCCATGTAGCC GATTTAGAAACAAGTTTAGATCCCCATCAAACGTTGTTGAAGGTGCAAAAATACAAACCC GCTTTAAGCGACTGGGTGCATTACATCTTCTTGGGATCCATCATGCTGTTTGTGTTCATT ACTAATCCCGCACCTTGGATCTTCAAGATCCTTTTTTATTGTTTCTTGGGCACTTTATTC ATCATTCCAGCTACGTCACAGTTTTTCTTCAATGCCTTGCCCATCCTAACATGGGTGGCG CTGTATTTCACTTCATCGTACTTTCCAGATGACCGCAGGCCTCCTATTACTGTCAAAGTG TTACCAGCGGTGGAAACAATTTTATACGGCGACAATTTAAGTGATATTCTTGCAACATCG ACGAATTCCTTTTTGGACATTTTAGCATGGTTACCGTACGGACTATTTCATTATGGGGCC CCATTTGTCGTTGCTGCCATCTTATTCGTATTTGGTCCACCAACTGTTTTGCAAGGTTAT GCTTTTGCATTTGGTTATATGAACCTGTTTGGTGTTATCATGCAAAATGTCTTTCCAGCC GCTCCCCCATGGTATAAAATTCTCTATGGATTGCAATCAGCCAACTATGATATGCATGGC TCGCCTGGTGGATTAGCTAGAATTGATAAGCTACTCGGTATTAATATGTATACTACAGCT TTTTCAAATTCCTCCGTCATTTTCGGTGCTTTTCCTTCACTGCATTCCGGGTGTGCTACT ATGGAAGCCCTGTTTTTCTGTTATTGTTTTCCAAAATTGAAGCCCTTGTTTATTGCTTAT GTTTGCTGGTTATGGTGGTCAACTATGTATCTGACACACCATTATTTTGTAGACCTTATG GCAGGTTCTGTGCTGTCATACGTTATTTTCCAGTACACAAAGTACACACATTTACCAATT GTAGATACATCTCTTTTTTGCAGATGGTCATACACTTCAATTGAGAAATACGATATATCA AAGAGTGATCCATTGGCTGCAGATTCAAACGATATCGAAAGTGTCCCTTTGTCCAACTTG GAACTTGACTTTGATCTTAATATGACTGATGAACCCAGTGTAAGCCCTTCGTTATTTGAT GGATCTACTTCTGTTTCTCGTTCGTCCGCCACGTCTATAACGTCACTAGGTGTAAAGAGG GCTTAA
Protein Properties
Pfam Domain Function
Protein Residues401
Protein Molecular Weight45192.80078
Protein Theoretical pI6.02
Signalling Regions
  • None
Transmembrane Regions
  • 42-62
  • 65-85
  • 88-108
  • 156-176
  • 179-199
  • 246-266
  • 269-289
  • 292-312
Protein Sequence>Inositol phosphorylceramide synthase MANPFSRWFLSERPPNCHVADLETSLDPHQTLLKVQKYKPALSDWVHYIFLGSIMLFVFI TNPAPWIFKILFYCFLGTLFIIPATSQFFFNALPILTWVALYFTSSYFPDDRRPPITVKV LPAVETILYGDNLSDILATSTNSFLDILAWLPYGLFHFGAPFVVAAILFVFGPPTVLQGY AFAFGYMNLFGVIMQNVFPAAPPWYKILYGLQSANYDMHGSPGGLARIDKLLGINMYTTA FSNSSVIFGAFPSLHSGCATMEALFFCYCFPKLKPLFIAYVCWLWWSTMYLTHHYFVDLM AGSVLSYVIFQYTKYTHLPIVDTSLFCRWSYTSIEKYDISKSDPLAADSNDIESVPLSNL ELDFDLNMTDEPSVSPSLFDGSTSVSRSSATSITSLGVKRA
References
External Links
ResourceLink
Saccharomyces Genome Database AUR1
Uniprot IDP36107
Uniprot NameAUR1_YEAST
GenBank Gene IDU49090
Genebank Protein ID1515352
General Reference
  • Hashida-Okado, T., Ogawa, A., Endo, M., Yasumoto, R., Takesako, K., Kato, I. (1996). "AUR1, a novel gene conferring aureobasidin resistance on Saccharomyces cerevisiae: a study of defective morphologies in Aur1p-depleted cells." Mol Gen Genet 251:236-244.8668135
  • Heidler, S. A., Radding, J. A. (1995). "The AUR1 gene in Saccharomyces cerevisiae encodes dominant resistance to the antifungal agent aureobasidin A (LY295337)." Antimicrob Agents Chemother 39:2765-2769.8593016
  • Dujon, B., Alexandraki, D., Andre, B., Ansorge, W., Baladron, V., Ballesta, J. P., Banrevi, A., Bolle, P. A., Bolotin-Fukuhara, M., Bossier, P., et, a. l. .. (1994). "Complete DNA sequence of yeast chromosome XI." Nature 369:371-378.8196765
  • Nagiec, M. M., Nagiec, E. E., Baltisberger, J. A., Wells, G. B., Lester, R. L., Dickson, R. C. (1997). "Sphingolipid synthesis as a target for antifungal drugs. Complementation of the inositol phosphorylceramide synthase defect in a mutant strain of Saccharomyces cerevisiae by the AUR1 gene." J Biol Chem 272:9809-9817.9092515
  • Levine, T. P., Wiggins, C. A., Munro, S. (2000). "Inositol phosphorylceramide synthase is located in the Golgi apparatus of Saccharomyces cerevisiae." Mol Biol Cell 11:2267-2281.10888667
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258
  • Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956
  • Aeed, P. A., Young, C. L., Nagiec, M. M., Elhammer, A. P. (2009). "Inhibition of inositol phosphorylceramide synthase by the cyclic peptide aureobasidin A." Antimicrob Agents Chemother 53:496-504.19047657