GPI ethanolamine phosphate transferase 1 (P36051)

Identification
NameGPI ethanolamine phosphate transferase 1
Synonyms
  • Morphogenesis checkpoint-dependent protein 4
  • Supersecretion of u-PA protein 21
Gene NameMCD4
Enzyme ClassNot Available
Biological Properties
General FunctionInvolved in mannose-ethanolamine phosphotransferase act
Specific FunctionEthanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the first alpha-1,4-linked mannose of the glycosylphosphatidylinositol precursor of GPI-anchor. Ethanolamine phosphate on the alpha-1,4-linked mannose is essential for further mannosylation by GPI10 and is necessary for an efficient recognition of GPI lipids and GPI proteins by the GPI transamidase, for the efficient transport of GPI anchored proteins from endoplasmic reticulum to Golgi and for the physiological incorporation of ceramides into GPI anchors by lipid remodeling. Also involved in non-mitochondrial ATP movements across membrane and participates in Golgi and endoplasmic reticulum function, Also required for the incorporation of BGL2 into the cell wall
Cellular LocationEndoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Vacuole membrane; Multi-pass membrane protein
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG Reactions
PE(14:0/16:1(9Z)) + 6-O-[alpha-D-mannosyl-(1->4)-alpha-D-glucosaminyl]-O-acyl-1-phosphatidyl-1D-myo-inositol ↔ 6-O-{2-O-[(2-aminoethyl)phosphoryl]-alpha-D-mannosyl-(1->4)-alpha-D-glucosaminyl}-O-acyl-1-phosphatidyl-1D-myo-inositol
Metabolites
YMDB IDNameView
GO Classification
Component
endoplasmic reticulum membrane
cell part
membrane
organelle membrane
Function
catalytic activity
transferase activity
Process
metabolic process
macromolecule metabolic process
macromolecule modification
protein modification process
protein amino acid lipidation
GPI anchor biosynthetic process
Gene Properties
Chromosome LocationNot Available
Locus
Gene Sequence>2760 bp ATGTGGAACAAAACCAGAACGACGCTTCTGGCTGTTGGTGTCTTATTTCATTTATTTTAC CTATGGTCTATTTTTGATATCTATTTCATTTCACCGCTCGTTCATGGTATGAGCCCATAT CAAAGTACTCCAACCCCTCCTGCAAAGAGATTGTTTTTGATTGTCGGTGATGGTTTACGT GCAGATACCACTTTTGATAAAGTCACTCATCCAGTATCCGGAAAAACAGAATTTCTGGCA CCTTTTATTAGATCTTTGGTAATGAATAATGCCACCTACGGTATATCACATACCAGAATG CCAACTGAATCCCGTCCTGGTCATGTTGCTATGATTGCTGGGTTTTACGAAGATGTTAGT GCCGTCACAAAAGGTTGGAAGTCAAACCCTGTCAATTTCGATAGTTTTTTCAACCAATCT ACTCATACTTATTCATTCGGTTCACCTGACATTTTACCTATGTTCAAAGATGGCGCTTCT GACCCAAATAAAGTTGACACTTGGATGTATGATCATACTTTCGAGGATTTTACGCAATCT TCCATCGAGCTGGATGCTTTTGTCTTTAGACACTTGGATCAATTATTCCACAATTCCACA CTGAACTCAACATTGGATTATGAAATTAGGCAAGACGGTAATGTATTCTTTCTACATCTA CTAGGTTGCGATACTGCCGGACATTCTTATAGACCATATTCTGCCGAGTATTATGACAAT GTCAAATATATTGATGATCAAATCCCTATCCTTATAGACAAAGTCAACAAGTTTTTTGCG GACGACAAAACCGCATTTATTTTTACAGCAGATCATGGTATGAGTGCATTTGGATCACAT GGTGACGGTCATCCTAACAACACAAGGACCCCTCTTGTTGCTTGGGGTGCGGGTTTGAAT AAACCAGTACATAATCCTTTTCCGGTATCCGACAACTATACTGAAAATTGGGAGCTTTCG AGCATTAAAAGAAATGATGTCAAGCAAGCAGATATTGCTTCTTTAATGTCATACTTGATT GGTGTGAACTATCCTAAAAATTCAGTTGGTGAGTTACCAATAGCATATATCGATGGAAAA GAAAGTGACAAGCTTGCCGCATTGTACAACAACGCAAGAAGCATTTTAGAGCAGTACTTA GTCAAGCAAGATGAGGTAATAGACTCTCAATTTTTTTATAAGGAATACTTCAAGTTTGTT GAAAAGTCTCATTCACATTACTTAGAAGAGATAGAAACCTTAATTCAGCGTATATCTGAA GGAGAAAACTATTTGGAACAAGAAGCAATCACCCTTACAGAGGAATTAATGCAGATAACA TTGGAAGGTTTACATTATTTGACAACCTATAATTGGAGATTCATTAGAACTATTGTTACA TTTGGGTTTGTTGGGTGGATCTTTTTTTCTTTTATAATATTTTTGAAATCATTCATATTA GAGAATGTAATTGATGACCAAAAAGCGTCACCATTAAGCCATGCAGTATTTGGTTCCATA GGAATTTTACTAAATTGGATTTTGTTCTACCAACATTCTCCGTTCAATTTTTACATGTAC CTTCTTTTCCCATTATACTTTTGGAGCTATATTTTTACAAATAGATCCGTACTACGTTCA GGTATCAAGGAATTCTTCAAAGGTACCTCTCCTTGGAAAAGAGTTTTAATAACAATCTCT ATTATATCAGTTTATGAGGGAATTGTATATGGATTTTTCCATAGATGGACGTTTACGCTA ATTACAAATATATTGGCGTTTTACCCGTTTATTTGTGGGGTGAGAGAGCTATCCGTGAAT ATATTGTGGATCATAACTAGTGTTCTTTTATCTACATTTACCTTATTTGACGCTGTTAAA ATTGAGGACTTGAACCAGATACATCTAGCAGGGTTATTAATCATTCTCAGTGCCTTTTAT GCTCTTTACAAAATACATTCCAGGATAAATTCCTACACGCGTGCTATATTTGCCATTCAA ATTTCCTTGGTGGCTGCCATGTTGGCGGTTACTCATCGTTCAGTTATCTCTTTACAGCTA AGACAAGGGTTACCAAGAGAGTCACAGGTCGCTGGATGGATAATTTTTTTTGTATCTCTT TTTGTAATGCCAATTTTACATTATAGGAAGCCCAACAATGATTACAAAGTGAGATTATTG ATCATTTATTTAACCTTCGCACCATCCTTTATCATTTTGACTATATCATTCGAATCCCTT TTCTACTTCTTGTTCACTAGTTACATGGTACAATGGATTGAAATTGAGAACAAAATCAAA GAAATGAAGACCCAAAAAGATGAAAATTGGTTACAAGTGCTAAGAGTTTCAGTAATCGGG TTCTTTTTACTTCAAGTCGCATTCTTTGGAACTGGTAACGTCGCTTCAATCTCTTCATTT TCATTGGAGTCTGTTTGTAGATTGTTGCCAATTTTTGATCCTTTCCTGATGGGCGCATTA TTGATGTTGAAATTGATAATTCCCTACGGGCTATTGTCCACATGCCTAGGTATACTGAAT TTAAAACTTAACTTCAAGGACTACACAATCTCATCATTAATTATTTCCATGAGTGATATT CTGTCGTTGAATTTTTTCTACCTTTTAAGAACGGAGGGGTCGTGGTTGGATATTGGCATA ACCATTTCCAACTATTGTTTGGCGATCCTATCATCTTTGTTCATGCTTATTTTGGAAGTA CTCGGTCATGTGTTGCTAAAAAATGTCATCATACAGGATAAAACCAAAAAAACACAATAG
Protein Properties
Pfam Domain Function
Protein Residues919
Protein Molecular Weight105692.0
Protein Theoretical pI6.71
Signalling Regions
  • None
Transmembrane Regions
  • 10-30
  • 458-478
  • 489-509
  • 513-533
  • 554-574
  • 577-597
  • 599-619
  • 627-647
  • 656-676
  • 688-708
  • 721-741
  • 777-797
  • 808-828
  • 849-869
  • 886-906
Protein Sequence>GPI ethanolamine phosphate transferase 1 MWNKTRTTLLAVGVLFHLFYLWSIFDIYFISPLVHGMSPYQSTPTPPAKRLFLIVGDGLR ADTTFDKVTHPVSGKTEFLAPFIRSLVMNNATYGISHTRMPTESRPGHVAMIAGFYEDVS AVTKGWKSNPVNFDSFFNQSTHTYSFGSPDILPMFKDGASDPNKVDTWMYDHTFEDFTQS SIELDAFVFRHLDQLFHNSTLNSTLDYEIRQDGNVFFLHLLGCDTAGHSYRPYSAEYYDN VKYIDDQIPILIDKVNKFFADDKTAFIFTADHGMSAFGSHGDGHPNNTRTPLVAWGAGLN KPVHNPFPVSDNYTENWELSSIKRNDVKQADIASLMSYLIGVNYPKNSVGELPIAYIDGK ESDKLAALYNNARSILEQYLVKQDEVIDSQFFYKEYFKFVEKSHSHYLEEIETLIQRISE GENYLEQEAITLTEELMQITLEGLHYLTTYNWRFIRTIVTFGFVGWIFFSFIIFLKSFIL ENVIDDQKASPLSHAVFGSIGILLNWILFYQHSPFNFYMYLLFPLYFWSYIFTNRSVLRS GIKEFFKGTSPWKRVLITISIISVYEGIVYGFFHRWTFTLITNILAFYPFICGVRELSVN ILWIITSVLLSTFTLFDAVKIEDLNQIHLAGLLIILSAFYALYKIHSRINSYTRAIFAIQ ISLVAAMLAVTHRSVISLQLRQGLPRESQVAGWIIFFVSLFVMPILHYRKPNNDYKVRLL IIYLTFAPSFIILTISFESLFYFLFTSYMVQWIEIENKIKEMKTQKDENWLQVLRVSVIG FFLLQVAFFGTGNVASISSFSLESVCRLLPIFDPFLMGALLMLKLIIPYGLLSTCLGILN LKLNFKDYTISSLIISMSDILSLNFFYLLRTEGSWLDIGITISNYCLAILSSLFMLILEV LGHVLLKNVIIQDKTKKTQ
References
External Links
ResourceLink
Saccharomyces Genome Database MCD4
Uniprot IDP36051
Uniprot NameMCD4_YEAST
GenBank Gene IDZ26877
Genebank Protein ID407483
General Reference
  • Vandenbol, M., Bolle, P. A., Dion, C., Portetelle, D., Hilger, F. (1994). "DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae." Yeast 10 Suppl A:S35-S40.8091859
  • Dujon, B., Alexandraki, D., Andre, B., Ansorge, W., Baladron, V., Ballesta, J. P., Banrevi, A., Bolle, P. A., Bolotin-Fukuhara, M., Bossier, P., et, a. l. .. (1994). "Complete DNA sequence of yeast chromosome XI." Nature 369:371-378.8196765
  • Gaynor, E. C., Mondesert, G., Grimme, S. J., Reed, S. I., Orlean, P., Emr, S. D. (1999). "MCD4 encodes a conserved endoplasmic reticulum membrane protein essential for glycosylphosphatidylinositol anchor synthesis in yeast." Mol Biol Cell 10:627-648.10069808
  • Packeiser, A. N., Urakov, V. N., Polyakova, Y. A., Shimanova, N. I., Shcherbukhin, V. D., Smirnov, V. N., Ter-Avanesyan, M. D. (1999). "A novel vacuolar protein encoded by SSU21 / MCD4 is involved in cell wall integrity in yeast." Yeast 15:1485-1501.10514566
  • Storey, M. K., Wu, W. I., Voelker, D. R. (2001). "A genetic screen for ethanolamine auxotrophs in Saccharomyces cerevisiae identifies a novel mutation in Mcd4p, a protein implicated in glycosylphosphatidylinositol anchor synthesis." Biochim Biophys Acta 1532:234-247.11470244
  • Kalebina, T. S., Laurinavichiute, D. K., Packeiser, A. N., Morenkov, O. S., Ter-Avanesyan, M. D., Kulaev, I. S. (2002). "Correct GPI-anchor synthesis is required for the incorporation of endoglucanase/glucanosyltransferase Bgl2p into the Saccharomyces cerevisiae cell wall." FEMS Microbiol Lett 210:81-85.12023081
  • Zhong, X., Malhotra, R., Guidotti, G. (2003). "ATP uptake in the Golgi and extracellular release require Mcd4 protein and the vacuolar H+-ATPase." J Biol Chem 278:33436-33444.12807869
  • Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
  • Maneesri, J., Azuma, M., Sakai, Y., Igarashi, K., Matsumoto, T., Fukuda, H., Kondo, A., Ooshima, H. (2005). "Deletion of MCD 4 involved in glycosylphosphatidylinositol (GPI) anchor synthesis leads to an increase in beta-1,6-glucan level and a decrease in GPI-anchored protein and mannan levels in the cell wall of Saccharomyces cerevisiae." J Biosci Bioeng 99:354-360.16233801
  • Zhu, Y., Vionnet, C., Conzelmann, A. (2006). "Ethanolaminephosphate side chain added to glycosylphosphatidylinositol (GPI) anchor by mcd4p is required for ceramide remodeling and forward transport of GPI proteins from endoplasmic reticulum to Golgi." J Biol Chem 281:19830-19839.16704983
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956