Oxysterol-binding protein homolog 1 (P35845)

Identification
NameOxysterol-binding protein homolog 1
SynonymsNot Available
Gene NameOSH1
Enzyme ClassNot Available
Biological Properties
General FunctionInvolved in identical protein binding
Specific FunctionLipid-binding protein involved in maintenance of intracellular sterol distribution and homeostasis. Binds to phosphoinositides. May be involved in formation of PMN vesicles by altering the membrane lipid composition
Cellular LocationCytoplasm. Golgi apparatus membrane. Nucleus outer membrane.
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
GO Classification
Process
metabolic process
primary metabolic process
lipid metabolic process
steroid metabolic process
Gene Properties
Chromosome LocationNot Available
Locus
Gene Sequence>3567 bp ATGGAACAACCTGATCTATCGTCTGTGGCCATCAGTAAGCCGCTGCTGAAGTTGAAACTT CTCGACGCCCTTCGCCAGGGAAGTTTCCCCAACCTACAAGATCTCCTAAAGAAACAATTC CAGCCGCTAGACGACCCAAACGTCCAACAAGTGCTCCATCTCATGCTCCACTATGCCGTG CAAGTCGCCCCCATGGCTGTCATAAAGGAAATCGTCCATCATTGGGTCTCAACTACAAAC ACCACTTTTCTAAACATCCATCTTGATCTAAACGAACGGGACTCCAACGGCAACACCCCA TTGCACATCGCCGCCTACCAGTCCCGCGGTGATATCGTAGCCTTCCTCCTGGACCAACCA ACCATCAACGACTGCGTGCTCAACAACTCCCACTTGCAGGCCATCGAAATGTGCAAGAAC CTAAACATCGCGCAGATGATGCAGGTGAAACGCTCCACATACGTTGCAGAGACCGCCCAG GAATTCAGAACAGCTTTTAACAACAGGGACTTCGGCCACCTAGAATCTATCCTCTCCAGC CCTCGAAACGCAGAACTGCTCGACATCAACGGTATGGACCCGGAGACTGGCGATACCGTT CTGCACGAATTCGTCAAGAAAAGAGACGTCATCATGTGCCGTTGGTTGCTTGAACACGGT GCTGACCCCTTCAAGAGAGACCGCAAGGGCAAACTGCCCATCGAGCTCGTTAGGAAAGTC AATGAAAACGACACCGCCACCAACACCAAGATCGCCATCGACATCGAACTGAAAAAACTA TTGGAAAGGGCCACCAGGGAGCAAAGTGTCATCGACGTCACAAACAACAACTTGCACGAG GCCCCCACTTACAAAGGCTACCTGAAAAAATGGACCAACTTCGCTCAAGGCTACAAATTG CGTTGGTTCATCCTTAGTAGCGATGGGAAACTATCCTACTACATCGATCAGGCCGACACT AAGAATGCCTGCAGGGGCTCCCTAAACATGTCTTCGTGCTCTCTGCATTTGGATTCGTCT GAAAAGTTGAAATTCGAAATTATCGGCGGTAACAACGGTGTTATCAGGTGGCATTTAAAG GGGAACCACCCCATCGAGACAAATAGATGGGTTTGGGCCATCCAGGGCGCCATAAGATAC GCAAAGGACAGAGAAATTTTGCTGCACAATGGCCCCTATTCGCCTTCTCTGGCCTTAAGC CATGGCTTGTCATCCAAAGTGTCCAATAAAGAAAACTTGCATGCAACTTCAAAACGGTTG ACCAAGAGCCCGCATCTGTCCAAATCCACACTGACACAAAACGATCACGATAATGACGAT GACAGCACTAACAACAACAACAACAAAAGTAATAATGATTATGACGATAATAATAATAAT AATAATAATGACGATGATGATTATGATGATGATGATGAAAGTAGACCCCTCATAGAACCA TTACCGTTGATTTCATCCAGAAGCCAAAGCTTAAGCGAAATCACTCCCGGTCCACATTCT AGGAAGTCTACAGTCTCGTCTACAAGGGCAGCCGATATACCATCAGATGATGAGGGTTAC TCTGAGGACGATTCTGATGACGACGGTAACTCCTCTTACACAATGGAAAACGGCGGTGAA AACGATGGCGACGAAGATCTAAATGCCATTTATGGTCCCTATATTCAAAAACTACACATG CTACAAAGATCCATTTCCATCGAGTTGGCATCTTTGAACGAATTGCTGCAAGATAAACAA CAACACGATGAGTACTGGAACACCGTCAACACTTCTATTGAAACCGTCAGCGAATTTTTC GACAAATTAAATCGGTTGACCTCTCAAAGGGAAAAAAGAATGATTGCCCAAATGACCAAG CAACGGGATGTTAACAATGTTTGGATTCAATCGGTAAAAGATCTGGAAATGGAACTGGTT GATAAAGACGAAAAATTGGTTGCCTTGGATAAAGAACGGAAAAATCTGAAAAAAATGCTT CAAAAAAAATTGAACAATCAACCACAGGTTGAAACTGAGGCTAATGAAGAATCCGATGAT GCAAATTCAATGATAAAAGGATCCCAAGAATCAACAAATACCCTTGAGGAAATCGTAAAA TTTATCGAAGCAACAAAGGAAAGTGATGAGGATTCTGACGCCGACGAATTTTTCGACGCA GAAGAAGCTGCTTCCGACAAAAAAGCCAATGATTCGGAAGACTTAACCACAAACAAGGAG ACTCCAGCTAATGCGAAACCACAAGAAGAAGCTCCTGAAGACGAGAGCCTTATTGTGATC AGTTCTCCACAGGTGGAAAAGAAGAACCAACTATTAAAAGAGGGATCATTCGTCGGATAT GAAGACCCAGTGAGAACCAAACTGGCTTTAGACGAAGATAATCGTCCCAAGATTGGTCTC TGGTCTGTTTTAAAGTCTATGGTCGGTCAAGACTTAACCAAACTAACTCTACCGGTATCG TTCAATGAGCCAACATCCTTACTACAGAGAGTATCTGAAGATATTGAGTATTCTCATATT CTTGACCAAGCTGCCACTTTTGAAGACTCCTCTTTAAGAATGCTATATGTAGCTGCCTTT ACTGCATCAATGTACGCATCTACCACTAACAGAGTGTCTAAACCATTCAACCCCTTACTC GGTGAAACTTTTGAATATGCCAGAACTGATGGTCAGTACCGATTCTTCACCGAACAAGTC TCTCACCACCCACCTATCTCTGCTACTTGGACAGAATCGCCCAAATGGGATTTTTACGGT GAATGTAATGTTGATTCGTCATTCAATGGGCGCACGTTCGCCGTGCAACATTTAGGATTA TGGTACATTACTATCCGGCCTGATCATAATATTAGTGTTCCCGAGGAAACTTATTCCTGG AAAAAACCAAATAACACTGTTATCGGTATTTTAATGGGGAAACCACAAGTAGACAACAGT GGGGACGTCAAAGTCACAAACCATACCACAGGCGACTATTGTATGCTGCATTACAAAGCC CATGGCTGGACCTCAGCCGGTGCATATGAAGTCAGAGGTGAAGTATTCAACAAGGACGAT AAAAAATTATGGGTTCTTGGTGGGCATTGGAATGATTCCATTTACGGGAAAAAAGTAACT GCTAGAGGCGGAGAACTGACATTAGACAGAATAAAAACGGCAAATTCTGCCACGGGAGGA CCAAAACTAGATGGGTCTAAGTTTCTGATATGGAAAGCAAATGAAAGGCCTTCAGTGCCA TTTAATTTAACGTCGTTTGCATTGACTTTGAATGCTTTGCCACCCCACTTGATACCATAT TTAGCACCCACAGATAGTCGTTTAAGGCCCGATCAAAGGGCTATGGAAAATGGTGAATAC GATAAAGCTGCCGCGGAAAAGCATCGTGTTGAAGTAAAACAAAGGGCAGCAAAAAAAGAA AGGGAACAAAAAGGAGAAGAATACAGACCTAAGTGGTTTGTCCAGGAGGAGCACCCCGTT ACCAAAAGTCTATACTGGAAATTTAATGGAGAGTATTGGAACAAAAGAAAAAATCATGAC TTTAAAGATTGTGCTGATATTTTCTAA
Protein Properties
Pfam Domain Function
Protein Residues1188
Protein Molecular Weight135096.0
Protein Theoretical pI5.26
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Oxysterol-binding protein homolog 1 MEQPDLSSVAISKPLLKLKLLDALRQGSFPNLQDLLKKQFQPLDDPNVQQVLHLMLHYAV QVAPMAVIKEIVHHWVSTTNTTFLNIHLDLNERDSNGNTPLHIAAYQSRGDIVAFLLDQP TINDCVLNNSHLQAIEMCKNLNIAQMMQVKRSTYVAETAQEFRTAFNNRDFGHLESILSS PRNAELLDINGMDPETGDTVLHEFVKKRDVIMCRWLLEHGADPFKRDRKGKLPIELVRKV NENDTATNTKIAIDIELKKLLERATREQSVIDVTNNNLHEAPTYKGYLKKWTNFAQGYKL RWFILSSDGKLSYYIDQADTKNACRGSLNMSSCSLHLDSSEKLKFEIIGGNNGVIRWHLK GNHPIETNRWVWAIQGAIRYAKDREILLHNGPYSPSLALSHGLSSKVSNKENLHATSKRL TKSPHLSKSTLTQNDHDNDDDSTNNNNNKSNNDYDDNNNNNNNDDDDYDDDDESRPLIEP LPLISSRSQSLSEITPGPHSRKSTVSSTRAADIPSDDEGYSEDDSDDDGNSSYTMENGGE NDGDEDLNAIYGPYIQKLHMLQRSISIELASLNELLQDKQQHDEYWNTVNTSIETVSEFF DKLNRLTSQREKRMIAQMTKQRDVNNVWIQSVKDLEMELVDKDEKLVALDKERKNLKKML QKKLNNQPQVETEANEESDDANSMIKGSQESTNTLEEIVKFIEATKESDEDSDADEFFDA EEAASDKKANDSEDLTTNKETPANAKPQEEAPEDESLIVISSPQVEKKNQLLKEGSFVGY EDPVRTKLALDEDNRPKIGLWSVLKSMVGQDLTKLTLPVSFNEPTSLLQRVSEDIEYSHI LDQAATFEDSSLRMLYVAAFTASMYASTTNRVSKPFNPLLGETFEYARTDGQYRFFTEQV SHHPPISATWTESPKWDFYGECNVDSSFNGRTFAVQHLGLWYITIRPDHNISVPEETYSW KKPNNTVIGILMGKPQVDNSGDVKVTNHTTGDYCMLHYKAHGWTSAGAYEVRGEVFNKDD KKLWVLGGHWNDSIYGKKVTARGGELTLDRIKTANSATGGPKLDGSKFLIWKANERPSVP FNLTSFALTLNALPPHLIPYLAPTDSRLRPDQRAMENGEYDKAAAEKHRVEVKQRAAKKE REQKGEEYRPKWFVQEEHPVTKSLYWKFNGEYWNKRKNHDFKDCADIF
References
External Links
ResourceLink
Saccharomyces Genome Database OSH1
Uniprot IDP35845
Uniprot NameOSH1_YEAST
GenBank Gene IDAY241177
Genebank Protein ID30421058
General Reference
  • Schmalix, W. A., Bandlow, W. (1994). "SWH1 from yeast encodes a candidate nuclear factor containing ankyrin repeats and showing homology to mammalian oxysterol-binding protein." Biochim Biophys Acta 1219:205-210.8086466
  • Loewen, C. J., Roy, A., Levine, T. P. (2003). "A conserved ER targeting motif in three families of lipid binding proteins and in Opi1p binds VAP." EMBO J 22:2025-2035.12727870
  • Bussey, H., Kaback, D. B., Zhong, W., Vo, D. T., Clark, M. W., Fortin, N., Hall, J., Ouellette, B. F., Keng, T., Barton, A. B., et, a. l. .. (1995). "The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Proc Natl Acad Sci U S A 92:3809-3813.7731988
  • Brachat, S., Dietrich, F. S., Voegeli, S., Zhang, Z., Stuart, L., Lerch, A., Gates, K., Gaffney, T., Philippsen, P. (2003). "Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii." Genome Biol 4:R45.12844361
  • Jiang, B., Brown, J. L., Sheraton, J., Fortin, N., Bussey, H. (1994). "A new family of yeast genes implicated in ergosterol synthesis is related to the human oxysterol binding protein." Yeast 10:341-353.8017104
  • Levine, T. P., Munro, S. (2001). "Dual targeting of Osh1p, a yeast homologue of oxysterol-binding protein, to both the Golgi and the nucleus-vacuole junction." Mol Biol Cell 12:1633-1644.11408574
  • Beh, C. T., Cool, L., Phillips, J., Rine, J. (2001). "Overlapping functions of the yeast oxysterol-binding protein homologues." Genetics 157:1117-1140.11238399
  • Beh, C. T., Rine, J. (2004). "A role for yeast oxysterol-binding protein homologs in endocytosis and in the maintenance of intracellular sterol-lipid distribution." J Cell Sci 117:2983-2996.15173322
  • Kvam, E., Goldfarb, D. S. (2004). "Nvj1p is the outer-nuclear-membrane receptor for oxysterol-binding protein homolog Osh1p in Saccharomyces cerevisiae." J Cell Sci 117:4959-4968.15367582
  • Yu, J. W., Mendrola, J. M., Audhya, A., Singh, S., Keleti, D., DeWald, D. B., Murray, D., Emr, S. D., Lemmon, M. A. (2004). "Genome-wide analysis of membrane targeting by S. cerevisiae pleckstrin homology domains." Mol Cell 13:677-688.15023338
  • Gruhler, A., Olsen, J. V., Mohammed, S., Mortensen, P., Faergeman, N. J., Mann, M., Jensen, O. N. (2005). "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Mol Cell Proteomics 4:310-327.15665377
  • Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
  • Chi, A., Huttenhower, C., Geer, L. Y., Coon, J. J., Syka, J. E., Bai, D. L., Shabanowitz, J., Burke, D. J., Troyanskaya, O. G., Hunt, D. F. (2007). "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Proc Natl Acad Sci U S A 104:2193-2198.17287358
  • Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956